Trim66 (NM_001170912) Mouse Untagged Clone

CAT#: MC224294

Trim66 (untagged) - Mouse tripartite motif-containing 66 (Trim66), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trim66
• Synonyms: Kiaa0298-hp; Tif1d
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224294 representing NM_001170912
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCCAGGCCTGCCTGTGAGCATTCCAAGTCAGCCACACTGCTCTACAGATGAACGCGTGGAGGCTC
  TGGCACCGACCTGCTCTATGTGTGGACGGGACCTACAAGCTGAGGGCTCCCGGCTTTTACCGTGCCAACA
  TCTGCTCTGTAAGGATTGCTACCAGGGCTTCATGCAGGAGCTAGGGCATGCTACCAGGGCTTACCCAGGT
  AAGCTCATCTCCTGTCCTGGATGTCAGCGTGTCTATCTTACCAGAGATGTAACTGAACATATTTTCCTTC
  AGTGCTTTTCTCCTGTGAAACCCACTATGGCCAGGAACTGCTCTGAGTGCAAGGAGAAGCGGGCAGCACA
  CATCCTCTGCACATACTGCAACCGCTGGCTGTGCAGCTCCTGCACAGAGGAGCACCGGCATGTCCCAGCC
  CCGGGGGGCCCACTCTTTGCACGGGCACAGAAGGGATCCTCAGGGGTGAATGGTGGCTCTGGGGACTTTG
  CCTTGTACTGCCCTCTGCACACACAGGAAGTGCTCAAGCTTTTCTGCGAGACGTGCGATGTACTCACCTG
  CCACAGCTGCCTGATGGTGGAGCACAAAGAGCACAGATGCAGGCACGTGGAAGAAGTTCTACAAAACCAG
  AGGATGCTCCTGGAGAGTGTGACTTCCCAGGTGGCACATAAGAAATCCAGCCTACAGACATCTGCAAAGC
  AAATCGAGGACAGAATTTTTGAAGTGAAGCATCAGCACCGGAAGGTGGAGAACCAGATTAAAATGGCCAA
  GATGGTCCTCATGAACGAGTTGAACAAGCAAGCCAACGGGCTCATAGAGGAGCTTGAGGGCATCACTAAT
  GAGAGAAAGCGAAAGCTGGAGCAGCAGCTGCAGAGCATCATGGTTCTCAACCGGCAGTTTGAACATGTGC
  AGAATTTCATCAACTGGGCTGTCTGCAGCAAAAGCAGCGTCCCTTTCCTTTTCAGCAAAGAGCTGATTGT
  GTTTCAGATGCAGCGGTTGCTGGAGACGAGATGTAACACAGATCCTGGCTCTCCTTGGAGTATCAGATTC
  ACCTGGGAGCCCAACTTCTGGACCAAGCAGCTGGCTTCCCTTGGCTGCATAACCACTGAAGGTGGACAGC
  TGACCCGGGCAGATGCTGCAGCTGCCTCTTATGGGAGTTTACAGGGGCAGCCATCTTTTTATCAGAGCCA
  CCAGGCCCCCATGGCTCAGCAGGAGGCACTCAGCCACCCCTCACACAAGTTCCAGTCTCCAGCACTGTGT
  TCCTCGTCTGTCTGCTGCTCCCACTGCTCTCCAGTCTCGCCTTCCCTCAAAGGTCAAGTCCCTCCACCCA
  GCATCCACCCGGCTCACAGTTTCCGACAGCCTTCTGAAATGGTGCCCCACCAGCTGGGGTCACTGCAGTG
  TTCTACCCTGCTGCCCAGGGAGAAAGAGCTGGCCTGCAGTCCTCATCCACCCAAACTGATGCAGCCCTGG
  TTGGAACCACAACCTCCTGCAGAACAGGAGAGTACATCCCAGCGGCCAGGACCGCAACTGGTTTCCCAGC
  CTGTGTGCATCGTCCCTCCGCAAGATGTGCAGCCAGGAGCCCATGCCCAGCCCACCATACAGACACCCTC
  CATCCAAGTTCAGCTGGGCCACCATCAGAAGCTAAAGCTCAGCCACTTTCAGCAGCAGCCGCAGCAGCAG
  CCACCCCCACCACCGCCACCTCCCCCACCCCCCCAGCATGCACCTCCACCCTTGCCTCCATCCCAGCACC
  TGGCTTCCAGTCAGCACGAGAGCCCTCCTGGGCCTGCCTGTTCGCAGAATGTGGACATTATGCACCACAA
  GTTTGAGCTGGAGGAGATGCAGAAGGACTTGGAGCTTCTCCTGCAGGCCCAGCAGCCTAGCCTGCAGTTG
  AGTCAGACCAAGTCTCCTCAGCATCTTCAGCAAACCATCGTGGGGCAGATCAACTACATCGTGAGGCAGC
  CAGCACCCGTGCAGTCCCAGAGCCAGGAGGAGACCCTGCAGGTTACAGAGGAGCCACCAGCACCTGAGGG
  CCCAAAGCCAGCTCTACCTGTTGACAAGAACACTGCTGCTCCCTTGCCCCAGACATCTGGGGAGGAAACG
  CCTCACAGTGTCCCTCCAGTGGATGGCACCTCCCAGCACTCTTCTCCAAATGTGGTGAGAAAGCACGCCA
  CCTCAGTGAGCATCATGGGCTTTTCCAACACTGTGGAGATGGAGTTGTCATCTACCAGGCTGGCGAGGAC
  CATAGAGCCACAGATACACAGGGTGAGCAGCCTGACAGCTGCCCCCACCCACACAATTCCGAGCTTGCTG
  AGTGGCCCACCACAAACTGTGTCCAGCCTGATGAGTGTTTCAAACCATGCCATGCCAAGCCTGACAGCCA
  GTCACCTGCAGCCCGTGCCAAACCTTGTGCGTGGCACATTCCAGTCCACGTCGAACCTGAGGGGTGATTC
  CTCTCAAGCCATCACAGGCCTGGCAAGTAATCACTCACAAGCTGGGCCCAGCCTAATGTCTGGGCACACC
  CAGGCTGCGCCGAGTCTGGCCACTTGTCCTCTGCAGGGAATGCCACCGGTTTCTGATGTGCATGTGGAGC
  CGAGATCCGTATCCAGTCCTGGGTCTGGCCCAGCTGCAGAAAGCCTGGGTACCAGGGATGGGGCTGAGTC
  CTCCCTGGGGAATGCCTTGTGTAAGATGGAAAGTGAGGATTGTACCCGTTTCTCAGACTCAGTAGGACAA
  GGCCCTACAGCCTCCAGTCTGGATGGTCCCAAGGATTTGGCTATTCCCTCAGAACTGGAGGAACCAATTA
  ACCTCTCTGTGAAAAAACCTTTCCTGGCACCAGTGATCAACACATCCACTGCTCTGCAACAGTACCGGAA
  TCCAAAAGAATATGAGAATTTTGAACAAGGAGCCCTAGAGCTGGATACAAAGGAGAATTCAGACATCAGA
  GCTATCAGTAGTGAGCCCAAGATCCCCTACGTGCGACTGGAGCGGCTCAAGATCTGTGCTGCCTCATCAG
  GAGAGATGCCTGTGTTTAAGCTGAAGCCCCAGAAAAATAGCCAGGACGGAAACTTCCTCCTGGTGATTGA
  GTGTGGCACTGAGTCCTCCAGCATGTCCATTAAGGTCAGCCAGAACAGCCTGCCTGATGCCAGCCAGGGC
  CCAGGTCTTGGGGGAAGAAAGGTCACTGTCACATCTCTGACTGGGCAGCAGCCACAAGAGGTGGAGAGCA
  CATCTGAGGAACACAGACTCATCCCCCGAGCTCCAGGAGCCAAGAAGAACACCCCGGCCCCCATAGAGAA
  TGAGGACTTCTGTGCGGTGTGCATCAACGGTGGGGAGCTGCTATGCTGTGACCGCTGCCCCAAAGTGTAT
  CACCTTTCCTGCCACGTGCCAGCCTTGCTCAGCTTCCCAGGGGGAGAATGGGTGTGCACCCTGTGCCGCA
  GCCTGACGCAGCCTGAGATGGAGTACGACTGTGAGAACGCGCGCTACGGTCACCCTGGAGTGCGGGTACT
  TCCCGGCCTGAGCATGTATGACCAGAAGAAGTGTGAGAAGCTGGTCCTGTCCCTGTGCTGCAACAGCCTC
  AGCCTGCCCTTCCATGAACCAGTCAGCCCTCTGGCCAGGCATTATTACCAGATTATCAAGAGGCCCATGG
  ACCTGTCAATCATCCGGAGGAAACTGCAAAAGAAGGACCCAGCTCATTACACTACTCCAGAGGAAGTGGT
  GTCAGATGTACGCCTCATGTTCTGGAACTGTGCTAAGTTCAATTATCCTGACTCGGAGGTTGCAGAGGCT
  GGCCGCTGTCTGGAAGTGTTCTTTGAAGGCTGGCTAAAGGAGATCTACCCAGACAAATGCTTTGCCCAGC
  CCCAGCAAGAGGATTCAGACTCTGAGGACGTATCTGGCGAGAGTGGCTGTTCCACTCCTCAGGGCTTCCC
  ATGGCCTCCCTACATGCAGGAAGGCATCCAGCCCAAGAGGCGACGGCGGCATATGGAGAATGAGAAGACA
  AAGAGAGTGTCGTTTCGTCTGGCCAACAGCATCTCGCAGGTGTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001170912
• Insert Size: 4035 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001170912.1, NP_001164383.1
• RefSeq Size: 9192 bp
• RefSeq ORF: 4035 bp
• Locus ID: 330627
• Cytogenetics: 7 57.21 cM
• Gene Summary: May function as transcription repressor; The repressive effects are mediated, at least in part, by recruitment of deacetylase activity. May play a role as negative regulator of postmeiotic genes acting through CBX3 complex formation and centromere association.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.