Mapk8ip3 (NM_013931) Mouse Untagged Clone

CAT#: MC224282

Mapk8ip3 (untagged) - Mouse mitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mapk8ip3
• Synonyms: BB120594; D17Wsu15e; JIP-3; Jip3; JSAP1; JSAP1a; JSAP1b; JSAP1c; JSAP1d; mKIAA1066; Syd2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224282 representing NM_013931
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGAGATCCAGATGGACGAGGGAGGAGGAGTGGTGGTGTACCAAGACGACTACTGCTCGGGCTCGG
  TCATGTCGGAGCGTGTGTCGGGCCTGGCGGGCTCCATCTACCGCGAGTTCGAGCGCCTCATTCACTGCTA
  TGACGAGGAGGTGGTCAAGGAGCTCATGCCGCTGGTGGTGAACGTGCTGGAGAACCTTGACTCGGTGCTG
  AGCGAGAACCAGGAGCACGAGGTGGAGCTGGAGCTCCTACGCGAGGACAACGAGCAGCTGCTCACGCAAT
  ACGAGCGCGAGAAGGCGCTGCGCAAACAGGCCGAGGAGAAATTCATCGAATTTGAAGATGCCTTGGAACA
  AGAGAAGAAAGAACTCCAGATCCAGGTAGAACATTATGAGTTTCAGACACGCCAGCTGGAGCTAAAGGCC
  AAAAACTATGCAGATCAGATTTCCCGACTGGAGGAACGAGAATCGGAGATGAAGAAGGAATACAATGCCC
  TGCACCAGCGGCACACAGAGATGATCCAGACCTATGTGGAACACATTGAAAGATCCAAGATGCAGCAAGT
  TGGGGGTAGCGGCCAAACAGAAAGCAGCCTGCCCGGGCGGAGCAGGAAGGAGCGTCCCACCTCTCTGAAT
  GTCTTCCCCCTGGCTGATGGCATGGTACGTGCACAGATGGGGGGCAAGCTCGTGCCTGCGGGGGACCACT
  GGCACCTGAGTGACCTCGGCCAGCTACAGTCCAGCTCCAGCTACCAGTGTCCAAATGATGAGATGTCTGA
  GTCAGGCCAGTCCTCAGCAGCTGCAACACCCAGTACCACAGGTACCAAGTCCAACACACCCACGTCCTCC
  GTGCCCTCAGCAGCAGTCACGCCACTCAACGAGAGCCTACAGCCCCTGGGGGACTATGTCAGTGTCACAA
  AGAACAACAAGCAGGCCCGAGAGAAGCGCAATAGCCGTAACATGGAGGTCCAGGTCACCCAAGAGATGCG
  GAACGTCAGTATCGGCATGGGCAGCAGTGACGAGTGGTCCGATGTTCAGGACATTATCGACTCCACCCCA
  GAGCTGGATGTGTGTCCTGAAACCCGTCTGGAGCGCACAGGAAGCAGCCCAACCCAGGGAATTGTAAACA
  AAGCTTTTGGAATCAACACTGACTCCTTGTATCACGAACTCTCCACGGCGGGATCTGAGGTCATCGGGGA
  TGTGGACGAGGGAGCTGATCTCCTAGGGGAGTTTTCAGTGCGCGATGATTTTTTTGGAATGGGCAAAGAA
  GTGGGGAACCTGCTGCTGGAGAACTCACAGCTTCTAGAGACAAAAAATGCTTTAAATGTAGTGAAGAATG
  ACCTCATTGCTAAGGTTGACCAACTGTCAGGAGAACAGGAGGTCCTGAAGGGTGAGCTGGAAGCAGCCAA
  GCAAGCCAAAGTCAAGCTGGAGAACCGAATCAAAGAGCTTGAAGAAGAACTGAAGAGAGTCAAGTCAGAG
  GCAGTAACTGCCCGCCGTGAGCCCAGAGAAGAGGTGGAGGATGTAAGCAGCTATCTCTGTACAGAATTGG
  ACAAAATCCCCATGGCCCAGCGCCGACGCTTCACACGGGTGGAGATGGCCCGAGTGCTCATGGAACGCAA
  CCAGTACAAGGAACGCCTCATGGAGCTGCAGGAGGCTGTGAGGTGGACTGAAATGATCAGAGCATCAAGG
  GAACACCCATCTGTCCAGGAGAAGAAGAAGTCCACCATCTGGCAGTTCTTTAGTCGCCTCTTCAGCTCCT
  CATCTAGCCCCCCTCCGGCCAAACGATCCTACCCATCTGTGAACATTCACTACAAGTCACCCACTGCAGC
  TGGCTTTAGCCAGCGTCGCAGCCATGCTTTGTGCCAGATCTCAGCCGGCAGCAGGCCCCTGGAGTTCTTC
  CCTGATGATGACTGCACCTCTTCTGCCCGGCGGGAGCAGAAGCGGGAGCAGTACCGCCAGGTTCGTGAAC
  ACGTGCGCAATGATGACGGGAGGCTGCAGGCCTGTGGGTGGAGCCTGCCTGCCAAGTACAAGCAGCTGAG
  CCCCAATGGAGGCCAGGAAGACACCCGGATGAAAAATGTGCCTGTCCCTGTGTACTGCCGCCCTCTGGTG
  GAGAAGGACCCTTCGACAAAGCTGTGGTGTGCTGCTGGTGTCAACCTGAGTGGGTGGAAGCCACATGAAG
  AGGACTCTAGCAATGGACCCAAGCCTGTACCAGGTCGAGACCCTCTGACCTGTGACCGGGAAGGAGAAGG
  CGAACCCAAGAGCACACACCCATCACCTGAGAAGAAGAAGGCAAAGGAAACCCCTGAGGCAGATGCTACC
  TCCAGTCGGGTATGGATCCTCACCAGCACCCTGACAACCAGCAAGGTGGTGATCATTGATGCCAACCAGC
  CAGGCACAATTGTGGATCAGTTCACAGTCTGCAATGCCCACGTCCTGTGTATCTCCAGCATTCCTGCGGC
  CAGTGACAGTGACTATCCCCCTGGGGAGATGTTCCTAGACAGTGATGTGAACCCTGAAGATTCAGGTGCT
  GATGGTGTGCTGGCTGGCATCACCCTGGTGGGGTGTGCTACCCGCTGCAATGTTCCACGTAGCAACTGTT
  CCTCACGAGGAGACACCCCAGTACTGGACAAGGGGCAGGGGGATGTGGCGACCACTGCCAATGGGAAGGT
  CAACCCGTCCCAATCCACAGAAGAAGCCACAGAAGCCACGGAGGTGCCAGACCCTGGTCCCAGCGAGTCA
  GAAGCAACGACAGTCCGGCCCGGGCCTCTCACAGAGCATGTCTTTACTGACCCAGCACCCACCCCATCCT
  CCAGCACCCAGCCTGCCAGTGAGAATGGGTCAGAGTCCAATGGCACCATTGTACAGCCTCAGGTGGAGCC
  CAGTGGGGAACTCTCAACAACAACCAGTAGCGCTGCACCCACTATGTGGCTAGGAGCCCAGAATGGCTGG
  CTCTATGTGCATTCAGCGGTAGCCAACTGGAAGAAGTGTCTGCACTCCATCAAGCTAAAAGACTCTGTGC
  TGAGCCTGGTGCATGTCAAAGGCCGAGTGCTGGTAGCTCTTGCAGATGGGACCCTGGCTATCTTCCATCG
  TGGAGAGGATGGCCAGTGGGACCTGAGCAACTACCACCTAATGGACCTGGGCCACCCACACCACTCCATC
  CGCTGCATGGCTGTTGTGAATGACCGAGTTTGGTGTGGCTACAAGAACAAGGTGCATGTTATCCAGCCCA
  AGACAATGCAGATTGAGAAATCATTTGATGCCCACCCAAGGCGGGAAAGCCAGGTACGTCAGCTGGCCTG
  GATCGGTGATGGAGTGTGGGTCTCTATTCGCTTGGATTCTACCCTTCGGCTCTACCATGCTCACACCCAC
  CAGCACCTGCAGGATGTGGACATTGAGCCCTATGTTAGCAAGATGCTAGGAACCGGCAAGCTGGGCTTCT
  CCTTCGTGCGCATCACAGCCTTACTCATTGCAGGCAACCGTCTGTGGGTGGGCACTGGCAATGGGGTTGT
  CATCTCCATCCCCTTGACTGAGACTGTGGTCCTGCATCGAGGCCAGCTCCTAGGGCTCCGAGCCAACAAG
  ACATCCCCAACATCTGGGGAGGGGACCCGCCCAGGGGGCATCATCCATGTGTATGGGGACGACAGCAGTG
  ACAAGGCCGCCAGTAGTTTCATCCCCTACTGCTCCATGGCACAGGCTCAGCTTTGCTTCCATGGGCACCG
  TGATGCTGTCAAATTCTTTGTCTCTGTGCCAGGAAATGTGCTGGCCACTCTCAATGGCAGTGTGCTAGAC
  AGCCCATCAGAGGGCCCTGGGCCTGCTGCACCCGCTGCAGATGCTGAGGGCCAGAAGTTGAAGAATGCAC
  TGGTGCTGAGTGGTGGTGAAGGTTACATTGACTTCCGTATCGGAGACGGAGAGGATGATGAAACTGAGGA
  ATGTGCCGGGGACGTGAACCAGACAAAGCCCTCGTTGTCCAAGGCTGAGCGCAGCCACATCATCGTGTGG
  CAGGTGTCCTACACCCCTGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013931
• Insert Size: 4014 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013931.4, NP_038959.2
• RefSeq Size: 5596 bp
• RefSeq ORF: 4014 bp
• Locus ID: 30957
• UniProt ID: Q9ESN9
• Cytogenetics: 17 12.53 cM
• Gene Summary: The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins (PubMed:10523642, PubMed:10629060). Promotes neuronal axon elongation in a kinesin- and JNK-dependent manner (PubMed:23576431, PubMed:25944905, PubMed:28259553). Activates cofilin at axon tips via local activation of JNK, thereby regulating filopodial dynamics and enhancing axon elongation (PubMed:23576431, PubMed:25944905, PubMed:28259553). Its binding to kinesin heavy chains (KHC), promotes kinesin-1 motility along microtubules and is essential for axon elongation and regeneration (PubMed:23576431, PubMed:25944905, PubMed:28259553). Regulates cortical neuronal migration by mediating NTRK2/TRKB anterograde axonal transport during brain development (PubMed:23576431, PubMed:25944905, PubMed:28259553). Acts as an adapter that bridges the interaction between NTRK2/TRKB and KLC1 and drives NTRK2/TRKB axonal but not dendritic anterograde transport, which is essential for subsequent BDNF-triggered signaling and filopodia formation (PubMed:23576431, PubMed:25944905, PubMed:28259553).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.