Eif3a (NM_010123) Mouse Untagged Clone

CAT#: MC224280

Eif3a (untagged) - Mouse eukaryotic translation initiation factor 3, subunit A (Eif3a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Eif3a
• Synonyms: A830012B05Rik; Csma; Eif3; Eif3s10; mKIAA0139
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224280 representing NM_010123
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGCCTATTTCCAGAGGCCGGAAAATGCCCTCAAGCGCGCCAACGAATTTCTTGAGGTTGGCAAAA
  AGCAGCCTGCCCTGGATGTTCTTTATGATGTAATGAAAAGTAAGAAGCACAGAACATGGCAGAAGATACA
  TGAGCCAATTATGCTGAAATACTTGGAACTGTGTGTGGATCTTCGTAAGAGCCACTTGGCTAAGGAAGGG
  TTATATCAATATAAGAACATATGCCAACAGGTAAACATTAAATCTTTAGAAGATGTTGTTAGGGCATATT
  TGAAATTAGCAGAGGAAAAAACAGAAGCTGCTAAAGAAGAGTCCCAACAAATGGTGTTAGATATAGAAGA
  TCTGGATAATATTCAGACTCCTGAGAGTGTTCTCTTAAGTGCAGTAAGTGGGGAAGATACTCAGGATCGT
  ACTGACAGATTGCTACTGACTCCCTGGGTCAAGTTCCTGTGGGAATCATACAGGCAGTGTTTGGACCTTC
  TTCGAAACAATTCTAGAGTAGAGCGCCTTTACCATGATATCGCCCAACAAGCTTTCAAATTCTGCCTCCA
  GTATACTCGGAAGGCTGAGTTCCGCAAGCTATGTGACAACTTGCGAATGCACTTATCCCAGATTCAGCGC
  CACCATAACCAAAGCACAGCAATTAATCTTAATAATCCAGAGAGCCAGTCTATGCATTTGGAAACCAGAC
  TTGTTCAGTTGGACAGTGCTATCAGCATGGAATTATGGCAGGAAGCCTTCAAAGCTGTGGAAGATATTCA
  TGGACTATTTTCCTTGTCTAAGAAACCACCTAAGCCTCAGTTGATGGCAAATTACTATAACAAAGTTTCA
  ACAGTGTTTTGGAAATCTGGAAATGCTTTGTTCCATGCATCTACACTTCATCGTCTTTATCATCTGTCTA
  GAGAAATGAGAAAGAATCTTACACAAGAAGAGATGCAAAGAATGTCTACTAGAGTCCTTTTGGCGACTCT
  TTCCATTCCTATTACTCCTGAGCGAACTGATATTGCTCGACTTTTGGATATGGATGGTATTATAGTTGAG
  AAGCAGCGACGCCTTGCCACATTGCTAGGTCTTCAAGCCCCACCCACACGAATCGGCCTAATTAATGATA
  TGGTCAGGTTCAGTGTGCTACAGTATGTTGTCCCAGAAGTGAAAGACCTTTACAACTGGTTGGAGGTGGA
  ATTCAACCCACTAAAACTCTGTGAGAGAGTTACAAAGGTATTAAATTGGGTTAGGGAACAACCTGAAAAA
  GAACCAGAATTGCAACAATATGTACCACAACTCCAGAACAATACCATACTCCGCCTTCTGCAACAGGTGG
  CACAGATTTATCAGAGCATTGAGTTTTCTCGTTTGACTTCTCTGGTTCCTTTTGTTGATGCTTTCCAACT
  GGAACGGGCCATAGTAGATGCAGCCAGGCACTGTGACCTGCAGGTACGTATTGACCATACTTCCCGGACT
  CTGAGTTTTGGATCAGATTTGAATTATGCAACTCGAGAAGATGCCCCAGTTGGCCCTCATCTGCAGAGCA
  TGCCTTCAGAGCAGATAAGAAACCAGCTCACGGCCATGTCCTCAGTGCTTGCCAAAGCACTTGAAGTCAT
  CAGACCTGCTCACATTCTGCAAGAGAAAGAAGAACAGCATCAATTGGCAGTTAATGCATATCTTAAAAAT
  TCAAGAAAAGAGCACCAGAGGATCCTGGCTCGGAGACAGACAATTGAGGAAAGAAAAGAGCGTCTTGAGA
  GTCTGAATATTCAACGTGAGAAGGAAGAACTCGAGCAGAGGGAAGCTGAACTCCAGAAAGTACGAAAGGC
  TGAAGAAGAAAGGCTGCGCCAAGAGGCAAAGGAGCGAGAGAAGGAACGAATCCTTCAAGAACACGAGCAA
  ATCAAGAAGAAAACTGTTCGGGAGCGGTTAGAGCAGATCAAGAAGACAGAGCTGGGCGCCAAAGCATTTA
  AAGATATTGACATTGAAGACCTTGAAGAACTGGATCCAGATTTTATTATGGCCAAACAGGTTGAACAACT
  GGAGAAAGAAAAGAAGGAACTTCAGGAACGCCTGAAGAATCAAGAAAAGAAGATTGACTATTTTGAGAGA
  GCTAAGCGTTTGGAAGAAATTCCTTTAATAAAGAGTGCTTATGAGGAACAAAGGGTTAAAGACATGGACC
  TGTGGGAACAGCAAGAAGAAGAAAGAATCACTACAATGCAGCTAGAACGAGAAAAAGCTCTGGAGCATAA
  GAATAGGATGTCACGAATGTTGGAAGACAGAGATCTATTTGTGATGCGCCTCAAAGCTGCCCGGCAGTCT
  GTCTACGAGGAAAAACTGAAACAGTTTGAAGAGCGCTTAGCAGAAGAAAGGCATAGTCGCCTAGAAGATC
  GGAAAAGGCAGCGGAAAGAAGAACGCAAAATAACTTATTACAGAGAAAAGGAAGAAGAAGAGCAGAGGAG
  GGCAGAGGAGCAGATGCTCAAAGAGCGAGAAGAAAGAGAACGTGCTGAGAGAGCAAAACGGGAGGAAGAA
  CTTAGAGAGTATCAGGAGCGAGTCAAGAAACTAGAAGAAGTAGAAAGGAAAAAGCGGCAAAGAGAGCTGG
  AAATTGAAGAAAGGGAAAGGCGCAGAGAGGAAGAAAGGAGACTTGGTGATGATCCACTTTCTAGGAAGGA
  CTCTCGGTGGGGAGATAGAGATTCAGAAGGCACCTGGAGGAAAGGACCAGAAGCTGACTCTGAGTGGAGA
  AGAGGCCCACCAGAAAAGGAGTGGAGACGAGAAACTCGGGATGATGAGAGGCCTCACAGGAGAGATGAGG
  ATCGGCTGAGGCGCTTGGGGGGTGACGATGAAGAGAGAGAGTCTTCTCTTAGACCGGATGATGATCGTAT
  CCCCAGGCGTGGCCTGGATGATGACAGAGGGCCTAGACGTGGTCCCGATGAAGATAGATTTTCTCGTAGA
  GGAACAGATGATGACCGTCCTTCCTGGCGTAATGCAGATGATGATAGGCCTCCCAGGCGAATTGGTGATG
  ATGACAGGGGAAGCTGGCGTCACACAGATGACGACAGACCACCCAGACGGGGACTGGATGATGAGAGAGG
  AAGCTGGCGTACAGCAGATGAGGACAGAGGACCTAGACGAGGGATGGATGATGACCGGGGGCCAAGACGA
  GGAGGTGCTGATGATGAACGGTCATCCTGGCGGAATGCTGATGATGATAGGGGTCCCAGGAGAGGCATGG
  ATGATGATAGGGGTCCCAGGCGAGGGCTGGATGATGACCGAGGACCTTGGAGGAATGCTGCTGAAGATAG
  GATTTCCAGGAGAGGTGCAGATGATGACCGAGGGCCTTGGAGAAATATGGATGATGATAGGGTTCCTAGA
  AGGGGTGATGATGCAAGACCTGGTCCTTGGAGACCATTTGTCAAGCCAGGTGGATGGAGAGAGAAAGAAA
  AGGCTAGAGAAGAGAGTTGGGGTCCACCTCGAGAATCAAGACCATCAGAAGAACGTGAATGGGATAGAGA
  CAAAGAGAAGGACAGAGATAATCAAGATCGAGAGGAGAATGACAAAGACCTTGAACGAGATAGGGACAGA
  GAGAGAGATGGGGACCGGGAGGATCGATTCAGAAGACCCAGGGATGAAGGTGGCTGGAGAAGAGGACCAG
  CAGAAGAATCTTCAAGCTGGAGAGATTCAAGTCGCCGTGATGATAGGGACAGGGAAGACCGTCGACGGGA
  TAGAGATGATCGTCGTGATCTGAGAGACCTGAGAGACAGAAGGGATTTAAGAGATGATAGAGATCGGAGA
  GGACCTCCCCTCAGATCAGAGCGAGAAGAAGCAAGCTCTTGGAGACGCACTGATGACAGGAAAGATGACC
  GGACTGAAGAGAGGGATCCACCTCGTCGTGTTCCTCCCCCAGCTCTTTCAAGAGATCGAGAAAGAGAGCG
  AGAACGAGAAGGTGAGAAAGAGAAAGCATCCTGGAGAGCTGAGAAAGATAGGGAGTCCCTTCGTCGTACT
  AAGAATGAAACTGATGAAGATGGATGGACCACAGTACGACGTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010123
• Insert Size: 4035 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010123.3, NP_034253.3
• RefSeq Size: 5176 bp
• RefSeq ORF: 4035 bp
• Locus ID: 13669
• UniProt ID: P23116
• Cytogenetics: 19 D3
• Gene Summary: RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.[UniProtKB/Swiss-Prot Function]