Cobl (NM_172496) Mouse Untagged Clone

CAT#: MC224279

Cobl (untagged) - Mouse cordon-bleu (Cobl), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cobl
• Synonyms: 4732460E13; C530045F18Rik; mKIAA0633
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224279 representing NM_172496
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGCGCCGCGTGCACTGGCGGCCAAGCCCCCCACAGGAAGGAAGATGAAGGCCCGTGCTCCGCCAC
  CTCCTGGAAAGCCTGCTGCCCAGAATGTCCACAGCGAACAGAAACTGCCCCATGATGCCACCCTGGGATC
  CCAGCAAAGCCTGGTCTACATGAAGGAGGCACTGCAGAACAGCACCTTGGACATCACCGTGGTTCTGCCC
  AGTGGACTGGAGAAGCAGAGTGTGGTTAGTGGGAGCCACGCAATGATGGACCTACTGGTTGAACTCTGCC
  TTCAGAACCACCTGAATCCTTCTCACCACGTCCTAGAGATCTGGTCTTCTGAGACCCAACAGCCTTTGAG
  TTTTAAGCCAAATACGTTGATTGGGTCCTTGAATGTGCATACTGTACTTCTGAAAGAAAAAGTCCCTGAA
  GAAAGAGTTAAACCTGGCCTAACCAAGGCTCCTGAGAAATCTGTACGACTGGTAGTGAACTACCTGAGAA
  CGCAGAAGGCTGTCGTGCGGGTGAGCCCTGAGGTGCCACTACAGAATATTCTGCCAGTCATCTGTGCGAA
  GTGTGAGGTCAACCCAGAACACGTGATTCTGCTCAGAGACAATGTTGCCGGGGAGGAGCTGGAGCTGTCC
  AAGTCCCTGAATGAACTAGGGATCAAGGAGCTGTATGCGTGGGACAACAGAAGAGAAATGTTTAGAAAAT
  CATCACTTGGCAATGATGAGACAGACAAAGAGAAGAAAAAGTTTCTGGGATTTTTCAAAGCTAATAAAAG
  GAGCAACAGTAAGGCTGAGCATCTTGGGCTGTCAGGTGCAGATAGTGATGAGGACCCGGCGAAGTCAGCA
  TCTGGAGGGGACTTGAACGGCTGCGTAACAACACCCAACTCACCATCCTTGCATTCTCGGTCCCTAACAC
  TGGGTCCCTCCCTCTCCCTGGGCAACATCTCTGGAGTGTCTATGAAGTCAGACATGAAGAAGCGCAGAGC
  CCCTCCTCCTCCAAGTCCCAAGCTGCTGGGTCAAGACAAGGTATCAGAAAAGGCCTCCCTCAGCTCACAG
  GCTGATCTCCAGAAGAAGAAGAGGCGGGCGCCAGCTCCTCCTCCACCACAGCAGCCACCACCAAGTCCTG
  TGGTCCCCAACCGCAAGGAAGATAAGGAAGAGAACAGGAAGAGCACAGTGGGTGTTGGACGTCAGGTGCC
  ACAAAAGCCTCCCAGAGGCACAGCTCGGGGCCCACCCCAGTTAGTGCTCCCCCCGCCCCCACCCTACCCT
  CCTCCTGACACAGATGTGACAGAGCCTGTCACCTTTCCTGGGGAAGGCGCTGGTTCCGAGACCTCAGAGC
  TGAGACCCAAACTGAGTCTGCCTCTGGGGCCTGGCAGCCACTGCAGCATGGGTGGAGTCTCTCAGGTGCC
  AGCGGAGAGTGAGGAGACAGCATCTGAGGACACAACCGAGGACTCGGGGGTCATGAGCTCTCCCTCAGAT
  GCCATCTCCCTGGACTCACAACAAGACAGCATGAGATCCAAGGACAAATGGTCCACAGACCAGGAAGACG
  GCAGTGACCAGGACCTGGCTGGAACTCCAGAACTGGGTCCCCAAAAGAGCCCATCATGGGGGAAAAGTGG
  TTCAGGAAGCTCAATTCTAAGGACTGAGAAAGCCACCATGCCTACCAATGATGATGAAGACCTATTCATC
  ACTGGCCACTTACATCAGACCCTGGCAGAACTTGATGAAGACCTGGAAGGAATGGAAGAAAATTATGAAA
  CAGATACTAGCTCTCTAACCAACTCTGTCAATGGTGTATCCAACCACAGTCTGCAAGAAGCCATAATCCC
  TGATAGTGGCGTGGATGACATCCCAGTCACTTTTATCGGGGAAGTTTCTGATGAGCCTTTTGACTCAGGG
  CTGTTCTCCAGTAGATGTAACAATGCCACTACTTTTAACACGGGGAGCATTGCTAGCCAAAGATCTCATC
  TGTCCCCATCTCAGACTGAGCATAGCCAGCCATTCGTAAGGACAAGTAGAAAAGAGCCTGATCCCTCCCC
  TCCTTCCCAAGATAACAGGAAGAGAAATCAGCCAACCTTAGCCAACACATCTGAAAATGAGAATCCAGTT
  GAAACAGACCCCACAGTTACATCACTTGTTTCAAAGCTTTTAATAGATGACCCAAAAGCAAAGGATAAAG
  GCAAAGTGCATGGTTCTAGTCACAGTGAGAAGACACAGGCAGGTCATGGAATAAACTCACTGCGAGTGAA
  TCCAAGAGATGGTAAGGATGAGAGCTCCAATTCAGCACCCCCACCATGGTCTCATCATGGCCAGGCCTTA
  GGGGGAAGCTATGGACTCAAGTATGGCCTCACGACATATAAAATTGTTCCTCCCAAATCAGAGATGAGGT
  GTTATGACAGGGATGTGTCCCTCTCCACTGGTGCCATTAAGATTGATGAACTAGGGAATCTGGTGAGTCC
  TCACATGAACGGAAGCAGGACCATATCCCCACCATCAGCTGTTGTCGAAACAGACACTCCACCTATTGGA
  AAAGTCAAAGAGTTCTGGAGGCGCAACTCAATGGAAAAGTACCTTAATGGGCCGGCTGAGTGCACTATCA
  AAAGGGCCCCCTCCACCACTATCACTGCCACACCAGAAAAACCACAGCAAGATAATGGGATGAAGGCAGC
  CTTCACAGTGACCACTCCCCAGCAGCAGCCTGCCTCACAGGAATATGGGGCACACCTGGAGGAAGAGAGA
  AGCCGGCCACAGTCAGCAGTCTCTTGTTCTGTGAAAGTGCCAGCATCTAATCCCACAGACATCACATTCC
  TCAAGCCTCAAAGAAGAACATCCAGCCAGTATGTGGCCTCTGCTATTGCCAAGAAGATGGGGCCTCCAAA
  AGTCCATGCTGATGTGGTGAGACCACATAAGGCAACCACTGAACAGTGTCATGAAGAAGCAAAGCTGGCT
  AGATCTCCTCCCACCAGGAAAGATGATGCAGCTCCCAACCTACACTCAGAGGCAAGGCAGCATGAACATG
  GGACAAACCAGAGCTCTGTCTGCCTTCCCAGCAACCCTGGTGTGCAATTGCCAGCAGGAGGCCATCCCAA
  AGTAGAGGTCAACAGCACATATGGGAAGTCATCGACTCAAGACTATCCTGCTGCTGTCCACAGAAACTCC
  TATTTCCTCCCAGGCAGATCTTCCCATAGGGATCGTGTTTCTGTGGGACAGAGCTGTGGTTTCAATGAAA
  AGCAAACTACAAGTAACCAAAAGGCAAATTCAACATCTAACTTCTCGCAGGCACTGGACAAGGCGCATCC
  ACCCCCTCTGCTCTTGGCAGAGGCCCGTGACTCTGGGAGGATACTGATGAATGGCTCTGCACGAACCCCA
  GGAAATTGTGAGCCCCCCCACTCTCCAAAAGAATCCACCCTGACTAGCTATATTATCTTACAGACAGAGG
  AAAAGCCCAGTTCGTTATCTACAGATGGTCAAGACGCAGATGATACTCTACCGTCCAGCATTTTTGGGCC
  AAAGAAAAAGTTCAAGCCTGTCATTCAAAGGCCACTCCCAAAAGATGTATCCCTGCACAGTGCCCTGATG
  GAAGCTATCCACTCATCAGGAGGGAGAGAAAAGCTCCGGAAGACTGCAGAACAGACGTCAGAAGGAAGGC
  CAAAGAAACCATCCTACGTGGAGGCAGAGAGTGAGCGATCTGCCCTTCTGGCAGCCATTCGGGGTCACAG
  TGGGACTCTCAGCCTCAGGAAGGTGTCATCCCTTGCCTCTGAGGAGCTCCAGAGCTTCCGCAATGCTGCA
  CTCGGGGCTCCAGGTTTGGATAAACCTCAGCAAGAAGACCTTGGCCTTCCACCCCCACCTGCCCTGCCAC
  CACCACCCGCTCCAGCCCCCCAGGCTCCTTCAGCCTCCGTAACAGTTTCTAGGTTCAGCACCGGCACCCC
  CAGCAACTCAGTGAATGCTAGACAAGCCTTGATGGATGCCATCCGCTCGGGCACAGGAGCTGCAAGACTA
  AGAAAGGTTCCCTTGCTCGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172496
• Insert Size: 4014 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172496.3, NP_766084.3
• RefSeq Size: 5633 bp
• RefSeq ORF: 4014 bp
• Locus ID: 12808
• UniProt ID: Q5NBX1
• Cytogenetics: 11 7.3 cM
• Gene Summary: Plays an important role in the reorganization of the actin cytoskeleton. Binds to and sequesters actin monomers (G actin). Nucleates actin polymerization by assembling three actin monomers in cross-filament orientation and thereby promotes growth of actin filaments at the barbed end. Can also mediate actin depolymerization at barbed ends and severing of actin filaments. Promotes formation of cell ruffles. Regulates neuron morphogenesis and increases branching of axons and dendrites. Regulates dendrite branching in Purkinje cells.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).