Myo3b (NM_177376) Mouse Untagged Clone

CAT#: MC224276

Myo3b (untagged) - Mouse myosin IIIB (Myo3b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Myo3b
• Synonyms: A430065P19Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224276 representing NM_177376
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCAGCCTCGGAGGTGAGCGGTGAGCACACAGGAGACAAAACCGGGAAGCACCTGTATGGATTAT
  TTCACTATAACCCCATGATGCTTGGCCTGGAATCACTCCCCGATCCCATGGAGACCTGGGAGATTATAGA
  GACGATTGGCAAAGGCACTTATGGCAAGGTCTACAAGGTAGCCAACAAGAGAGATGGGAGCCTGGCTGCC
  GTGAAGGTTCTGGACCCCGTCAGTGATATGGATGAGGAGATTGAGGCTGAATACAACATTCTGCAGTTTC
  TCCCCAGCCATCCCAACGTTGTAAAGTTTTATGGGATGTTTTACAAAGCCGATCGCTGTGTGGGAGGACA
  GCTATGGCTGGTTCTGGAGCTGTGTAATGGGGGCTCTGTCACTGAGCTTGTCAAGGGCCTTCTGAGGTGC
  GGCAAGAGGCTGGACGAAGCTGTGATTTCCTACATTCTGTATGGAGCCCTCTTGGGCCTTCAGCATTTGC
  ACTGCCACCGAATCATCCACCGAGATGTGAAGGGGAATAACATTCTTCTGACGACAGAAGGAGGAGTTAA
  GCTCGTTGACTTTGGTGTCTCTGCTCAACTTACAAGCACACGGCTGCGGAGAAACACATCAGTTGGGACC
  CCATTCTGGATGGCTCCTGAGGTCATTGCTTGCGAGCAGCAGTATGACTCGTCCTATGACGCTCGTTGTG
  ACGTCTGGTCCTTGGGCATCACAGCCATTGAGCTGGGAGATGGAGACCCTCCCCTCTTTGAAATGCATCC
  TGTGAAAATGCTCTTTAAGATACCAAGAAACCCTCCACCCACTTTGCTGCACCCTGACAGCTGGTGTGAG
  GAATTTAACCACTTCATTTCACAGTGTCTTATTAAAGATTTTGAAAAGCGGCCTTCGGTCACCCATCTCT
  TGGACCACCCATTCATTAAAGGAACTCAAGGCAAGGTTTTGTGTCTGCAAAAACAGCTGGCCAAGGTGCT
  CCAGGACCAGAAGCATCGAAACCCTGTTGCTAAAACCAGGCATGAAAGGATGCATACTGGAAGACCCCAT
  CGAGTAGAGGATGCCGGCAAGTGCTGCCTTGAGGATGACCTGGTCAATCTCGAGGTCCTGGACGAGGACA
  CGATTATCTACTGGCTGCAGAAGCGGTATGCAGATGCACTGATCTACACCTATGTTGGAGACATTCTAAT
  TGCCTTAAACCCCTTCCAGAATCTAAGTATATACTCTCCACAGTTTTCCAGGCTTTATCATGGGGTGAAA
  CGTTCCTCGAATCCCCCCCACATATTTGCATCAGCAGACAACGCTTATCAGTGTTTGGTTACTTTCAGCA
  AAGACCAGTGTATTGTCATCAGTGGAGAAAGCGGATCGGGGAAGACGGAAAGCGCCCATCTGATTGTTCA
  GCATCTTACTTTCTTAGGAAAGGCTGACAATCAGACCCTACGCCAGAAAATCCTCCAAGTGAACTCCCTG
  GTGGAAGCCTTCGGGAACGCCCGCACAGCCATCAATGATAACTCCAGCCGCTTCGGGAAGTATCTAGAAA
  TGATGTTCACACCAACTGGAGCTGTCATGGGGGCAAGAATCTCCGAGTATCTTCTTGAGAAATCCAGAGT
  TATACAGCAGGCAGCGGGAGAGAAAAATTTTCATATATTTTACTATATTTATGCTGGTCTTTATCACCAA
  AAGAAACTCGCTGAGTTCAGACTTCCCGAGGAAAAGCCTCCTAGATATATAGCTGGGGAAACTGAAAGAG
  TCATGCAGGACATCACCTCCAAAGAGTCTTACCGGACACAGTTTGAGGCCATTCAGCACTGCTTCAAGAT
  TATTGGATTCGCTGACAAGGAGGTACACTCTGTGTACAGAATTCTGGCGGGGATTTTGAATATTGGCAGC
  ATTGAATTCGCAGCTATTTCCTCTCAACACCAAACTGATAAAAGTGAGGTACCTAATCCAGAAGCTCTGG
  AAAACGCTGCCTGCGTGCTCTGCATTAGCTCCGAAGAGCTTCAGGAGGCCCTCACTTCCCACTGTGTGGT
  CACACGAGGGGAGACCATTGTCAGAGCCAACACAGTGGACAGGGCTGAAGACGTCAGGGACGCCATGTCT
  AAGGCCCTCTATGGGAGGCTGTTCAGCTGGATTGTGAATCGAATCAACACACTCCTGCAGCCAGATAAAA
  ACATATGTAGTGCAGAGGACAGGATGAATGTGGGCATCTTGGACATTTTTGGCTTTGAGGATTTCCAGAG
  GAATTCCTTTGAACAGCTCTGCATAAATATTGCCAATGAACAGATCCAGTACTACTTCAATCAGCATGTG
  TTTGCTCTTGAGCAGATGGAGTACAAGAATGAAGGTGTCGATGCTGTCCTCGTGCAGTATGAAGACAATC
  GCCCACTCCTGGACATGTTCCTCCAAAAACCCTTGGGCCTTCTGGCACTTCTGGATGAAGAAAGTCGATT
  TCCCCAAGGGACAGACCAGACCTTGGTTGATAAATTTGAAGACAACCTACGGTGTAAGTTCTTCTGGAGG
  CCCAAAGGTGTGGAACTCTGTTTTGGCATTCAGCACTATGCTGGACCGGTGTTATACGATGCTTCTGGGG
  TTCTTGAGAAAAACAGAGACACCCTCCCTGCTGACGTGGTGGTAGTCCTGAGGACGTCTGAGAACAAACT
  CCTTCAGCAACTGTTCTCAATTCCTTTGACCAAAACAGGTAATTTGGCACAAACCAGAGCTAAGATCACA
  GCATCCTCGAGATCTTTGCCTCCACATTTCAGTGCTGGGAGGGCCAAGGTAGACACCCTGGAGGTGATTC
  GACATCCAGAAGAAACCACCAACATGAAGAGACAAACCATGGCTTCTTACTTCCGGTATTCTCTGATGGA
  CCTGCTCTCCAAAATGGTGGTCGGACAGCCTCACTTTATTCGTTGCATTAAGCCTAATGATGACCGCAAG
  GCCCTGCAGTTCTCCCAAGACAGAGTCTTGGCCCAGCTTCGCTCCACGGGCATCCTGGAGACCGTCAGCA
  TCCGCAGGCAGGGCTACTCCCATCGCATCTTCTTTGAAGAGTTTGTTAAAAGGTATTATTACTTGGCATT
  CCGAGCACATCAAACACCTCCTGCTAACAAAGAGAGCTGTGTTGCTATCTTGGAAAAGTCCAGATTAGAT
  CACTGGGTCCTGGGGAAAACAAAGGTTTTCCTCAAATATTACCATGTCGAGCAATTAAATTTGCTTCTGC
  GAGAAGTCATGGGACGAGTGGTCATGCTGCAGGCATATACCAAGGGCTGGCTCGGAGCAAGGAGATACAA
  GAGAGCCAAAGAGAAGAGAGAGAAGGGCGCCATCACCATCCAGTCAGCCTGGAGAGGCTATGATGCTCGG
  AGGAAATTGAAGCAAAGAAGCCGGAGGAGGAGTGAGTCCGAGGCTCACATTCACACAGTTCTCCAAACCA
  CTCCTGATCAGAAATACTGTCCAGATTCAGGAGGAGAGTCCAACAGAGGTCATGAAGAGACGTCAAGGAA
  CTGCCCCGCTGAAGCCGACACAGATGGGCACCCACAAGCCCAGAGTCCCCCAACAGGCTGTGATGTCACC
  TCAGGACATGCAGATACAGCAGCTGGGTATACTGTTGCTGAGCTCTCAGTTGCTGGAACCGATGTGTCAC
  CTTCTCTGGTGTACCACACTGCCTCAGCTCACCAAAGACTGAGCCCCTGTGAAGACTCTCTCAAACCTGG
  TTCCGAAGAAGGTCTCTCCCAGAAGCAGCGAGCACCTCGCAGACGGTGTCAGCAGCCCAAAATGTTGAGT
  AGCCCCGAGGACACCATGTACTATAACCAGTTAAATGGAACTCTAGAATATCAAGGGAGCCAGAGGAAGC
  CAAGAAAACTTGGACAAATCAAAGTGCTGGATGGGGAGGACCAGTATTACAAATGCCTGTCCCCTGGGGC
  CTGTGCCCCTGAGGAAACCCACTCAGTCCACCCTTTCTTCTTCTCCTCGTCACCCAGAGAAGACCCCTTT
  GCTCAACACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177376
• Insert Size: 4002 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177376.4, NP_796350.2
• RefSeq Size: 6256 bp
• RefSeq ORF: 4002 bp
• Locus ID: 329421
• UniProt ID: Q1EG27
• Cytogenetics: 2 C2
• Gene Summary: Probable actin-based motor with a protein kinase activity (By similarity). Required for normal cochlear hair bundle development and hearing. Plays an important role in the early steps of cochlear hair bundle morphogenesis. Influences the number and lengths of stereocilia to be produced and limits the growth of microvilli within the forming auditory hair bundles thereby contributing to the architecture of the hair bundle, including its staircase pattern (PubMed:26754646). Involved in the elongation of actin in stereocilia tips by transporting the actin regulatory factor ESPN to the plus ends of actin filaments (PubMed:22264607).[UniProtKB/Swiss-Prot Function]