Adgra2 (NM_054044) Mouse Untagged Clone

CAT#: MC224269

Gpr124 (untagged) - Mouse G protein-coupled receptor 124 (Gpr124), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Adgra2
• Synonyms: 8430414O08Rik; 9530074E10Rik; Gpr124; mKIAA1531; Tem5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224269 representing NM_054044
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGCGGGGGGACGCAGGATGCCGGTGCCTCCCGCGCGTTTGCTGCTGCTGCCGCTGCTGCCTTGTC
  TTCTGCTCCTGGCTCCTGGAACTCGGGGTGCGCCTGGCTGCCCGGTCCCTATCCGCGGTTGCAAGTGCTC
  TGGGGAGCGGCCCAAGGGACTAAGTGGCGGCGCCCACAACCCGGCTCGAAGGAGGGTGGTGTGCGGCGGT
  GGGGATCTCCCCGAACCTCCAGATCCCGGCCTTCTGCCAAACGGCACCATCACCTTGCTCTTGAGCAACA
  ACAAGATTACTGGGCTCCGCAATGGATCCTTCTTGGGACTGTCCCTGTTGGAGAAGTTGGACCTGAGGAG
  CAATGTCATCAGCACTGTGCAGCCTGGAGCCTTCCTAGGTCTGGGAGAGCTAAAACGCTTAGATCTCTCC
  AACAATCGGATTGGCTGTCTCACCTCTGAGACATTTCAAGGGCTCCCTAGACTTCTCAGACTAAACATAT
  CTGGAAACATCTACTCTAGTCTGCAACCTGGGGTCTTTGATGAGCTGCCAGCCCTTAAGATTGTGGACTT
  TGGTACTGAGTTTCTGACCTGTGACTGCCGCCTGCGCTGGCTGCTGCCCTGGGCCCGGAATCACTCCCTG
  CAGCTGTCTGAGCGCACACTCTGTGCCTACCCCAGTGCCCTGCACGCCCATGCCCTGAGCAGCCTCCAGG
  AGTCCCAGCTTCGCTGTGAAGGGGCCCTGGAACTGCACACCCACTACCTCATCCCATCCCTTCGCCAAGT
  GGTGTTCCAGGGTGACCGCCTGCCCTTCCAGTGCTCAGCCAGCTACTTGGGCAACGATACCCGGATCCAC
  TGGTACCACAATGGGGCTCCTATGGAGAGCGATGAGCAGGCCGGCATCGTCCTTGCTGAAAACCTTATCC
  ATGATTGCACCTTCATCACCAGTGAGCTGACCCTGTCTCACATTGGTGTGTGGGCCTCTGGTGAATGGGA
  GTGCTCCGTGTCCACAGTCCAAGGCAACACCAGCAAGAAGGTGGAGATAGTAGTGCTGGAGACCTCTGCC
  TCCTACTGCCCTGCAGAGCGGGTGACCAACAACCGTGGGGACTTCAGGTGGCCCCGAACCTTGGCTGGCA
  TCACGGCTTACCAGTCCTGTTTACAGTACCCCTTCACCTCTGTGCCCTTGAGTGGGGGAGCCCCGGGTAC
  CCGAGCCTCACGCAGGTGTGACCGAGCTGGCCGCTGGGAGCCAGGGGACTACTCGCACTGTCTGTACACC
  AATGACATCACTCGGGTGCTCTACACCTTTGTGCTGATGCCCATCAACGCCTCCAATGCATTGACGTTGG
  CCCACCAGCTGCGAGTGTATACCGCAGAGGCCGCCAGCTTCTCAGACATGATGGACGTGGTCTATGTGGC
  TCAGATGATCCAGAAGTTTTTGGGTTACGTTGACCAGATCAAGGAGCTGGTGGAGGTGATGGTGGACATG
  GCCAGCAACCTGATGCTGGTGGATGAGCACCTTCTGTGGCTGGCCCAGAGAGAAGACAAAGCCTGCAGTG
  GCATTGTGGGTGCCCTGGAGCGAATCGGAGGAGCTGCTCTTAGCCCCCATGCCCAGCACATCTCTGTGAA
  TTCAAGAAATGTGGCACTGGAGGCCTACCTCATCAAGCCTCACAGCTACGTGGGTCTGACTTGCACGGCT
  TTCCAGAGGAGGGAGGTAGGAGTGTCGGGTGCACAGCCAAGCAGCGTCGGCCAGGATGCCCCAGTGGAGC
  CCGAACCCCTAGCTGATCAGCAGCTTAGGTTCCGCTGCACCACTGGGAGGCCCAACATTTCTCTGTCATC
  CTTCCACATCAAGAATAGCGTGGCCCTGGCCTCCATCCAGCTGCCCCCCAGCCTGTTCTCAACCCTTCCG
  GCTGCCCTGGCTCCCCCAGTCCCTCCAGATTGCACCCTGCAACTGCTGGTCTTCAGAAATGGCCGTCTTT
  TCCGCAGCCACGGCAACAACACTTCCCGTCCTGGAGCAGCTGGGCCTGGCAAGAGGCGTGGTGTGGCCAC
  CCCAGTCATATTTGCAGGAACCAGTGGCTGTGGTGTGGGAAACTTGACGGAGCCCGTGGCTGTGTCACTG
  AGGCACTGGGCTGAAGGAGCTGACCCCATGGCAGCTTGGTGGAACCAGGACGGGCCTGGAGGCTGGAGTT
  CTGAAGGCTGCAGGCTCCGCTACAGCCAGCCCAACGTCAGCTCCCTGTACTGCCAGCACTTGGGCAATGT
  GGCCGTGCTTATGGAGCTGAATGCATTCCCGCGGGAGGCAGGAGGCTCTGGGGCTGGGTTGCATCCCGTC
  GTGTACCCCTGCACGGCTTTGCTGCTACTCTGTCTCTTCTCCACCATCATCACGTACATCCTCAACCACA
  GCTCCATCCACGTGTCCCGGAAGGGTTGGCACATGCTGCTGAACCTTTGTTTCCATATGGCCATGACCTC
  TGCTGTCTTCGTGGGGGGCGTCACGCTCACCAACTACCAAATGGTTTGTCAAGCGGTGGGCATCACTCTG
  CACTACTCTTCCTTGTCGTCACTGCTCTGGATGGGGGTCAAGGCTCGAGTCCTCCACAAGGAGCTTAGTT
  GGAGGGCACCCCCTCTGGAAGAAGGGGAAGCAGCTCCGCCTGGTCCTCGCCCCATGCTCCGGTTCTACTT
  GATTGCTGGAGGGATCCCCCTCATAATCTGCGGCATCACCGCTGCGGTCAACATCCACAACTACCGGGAC
  CACAGTCCCTATTGTTGGCTGGTGTGGCGTCCAAGCCTTGGTGCCTTCTACATACCGGTGGCGTTGATTC
  TGCCTATCACCTGGATCTACTTCTTGTGTGCAGGCCTTCACTTACGGAGCCATGTGGCCCAGAATCCAAA
  GCAGGGTAACAGGATCTCTCTGGAGCCAGGGGAAGAGCTGAGGGGTTCCACCAGGCTCAGGAGTAGTGGC
  GTCCTCCTGAATGACTCTGGTTCTCTTTTGGCTACAGTTAGCGCAGGAGTAGGGACACCTGCGCCCCCAG
  AGGATGGTGATGGCGTATATTCTCCTGGAGTCCAGCTGGGGGCGCTGATGACCACGCATTTCCTGTACCT
  GGCTATGTGGGCTTGTGGTGCCTTGGCGGTGTCTCAGCGCTGGCTGCCCCGAGTGGTGTGTAGCTGTCTG
  TACGGCGTGGCAGCTTCAGCTCTTGGTCTGTTTGTCTTCACTCACCACTGTGCCAGACGTAGAGATGTCC
  GGGCTTCCTGGCGCGCCTGCTGCCCTCCTGCTTCGCCCTCGGCCTCCCATGTCCCAGCCCGGGCCCTGCC
  GACTGCTACAGAGGATGGATCCCCAGTGTTGGGGGAGGGACCAGCCTCTCTCAAGTCCTCCCCAAGTGGC
  AGCAGTGGCCGCGCGCCGCCACCCCCCTGCAAACTCACCAATCTGCAGGTGGCCCAGAGTCAGGTGTGCG
  AGGCAAGCGTGGCCGCCCGCGGAGATGGAGAGCCAGAGCCCACCGGCTCCCGTGGCAGCCTAGCTCCCCG
  GCACCATAACAACCTGCATCATGGGCGCCGAGTACACAAGAGTCGGGCCAAGGGGCACCGAGCCGGAGAG
  ACTGGTGGTAAGAGCCGGCTCAAGGCGTTGCGCGCGGGCACGTCCCCAGGAGCTCCCGAGCTGTTGTCCA
  GTGAGAGCGGCAGTTTGCACAACAGCCCGTCTGACAGCTACCCGGGCAGCAGCCGCAACAGTCCAGGCGA
  CGGTCTTCCACTCGAGGGTGAGCCCATGCTTACGCCGTCGGAGGGCAGTGACACAAGCGCCGCGCCAATC
  GCTGAGACGGGGCGCCCCGGGCAGCGCCGCAGCGCCAGCCGTGACAACCTCAAGGGCAGCGGCAGCGCGC
  TGGAGAGGGAGAGCAAGCGCCGCTCCTATCCGCTCAACACTACCAGTCTTAACGGTGCCCCCAAAGGGGG
  CAAGTATGAGGATGCCAGTGTAACTGGTGCAGAGGCCATAGCGGGAGGCTCCATGAAGACTGGCCTCTGG
  AAAAGCGAGACCACCGTCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_054044
• Insert Size: 4011 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_054044.2, NP_473385.2
• RefSeq Size: 5520 bp
• RefSeq ORF: 4011 bp
• Locus ID: 78560
• UniProt ID: Q91ZV8
• Cytogenetics: 8 A2
• Gene Summary: Endothelial receptor which functions together with RECK to enable brain endothelial cells to selectively respond to Wnt7 signals (WNT7A or WNT7B) (PubMed:25373781, PubMed:25558062, PubMed:28803732). Plays a key role in Wnt7-specific responses, such as endothelial cell sprouting and migration in the forebrain and neural tube, and establishment of the blood-brain barrier (PubMed:21071672, PubMed:21282641, PubMed:21421844, PubMed:25373781, PubMed:28288111). Acts as a Wnt7-specific coactivator of canonical Wnt signaling: required to deliver RECK-bound Wnt7 to frizzled by assembling a higher-order RECK-ADGRA2-Fzd-LRP5-LRP6 complex (By similarity). ADGRA2-tethering function does not rely on its G-protein coupled receptor (GPCR) structure but instead on its combined capacity to interact with RECK extracellularly and recruit the Dishevelled scaffolding protein intracellularly (By similarity). Binds to the glycosaminoglycans heparin, heparin sulfate, chondroitin sulfate and dermatan sulfate (By similarity).[UniProtKB/Swiss-Prot Function]