Atp8b3 (NM_026094) Mouse Untagged Clone

CAT#: MC224261

Atp8b3 (untagged) - Mouse ATPase, class I, type 8B, member 3 (Atp8b3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Atp8b3
• Synonyms: 1700042F02Rik; 1700056N23Rik; ATPIK; SAPLT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224261 representing NM_026094
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGGAGTTCATCTCGGGGAGAACCTTGAGGATAAAGACACAGAGTTCACCTGGGAGGTGAAGGCCA
  ATGACAGAACCTATCACAAGCAGTTCAAGAAGAAGGGCTTTCTATGCTGGAGGCAGAAGAAGTACAAGAG
  CAACGCCATCCACACTGCCAAATACAACATCTTCTCCTTCCTGCCTCTGAACCTGTACGAGCAGTTCCAT
  CGAATGTCCAATCTCTACTTCCTTTTTATCATCATCCTGCAGGGCATCCCCGAGATCTCCACACTGCCCT
  GGTTCACGCTCTTTGCACCTCTGGTCTGCCTGTTTGTGATCCGGGCCACTCGTGATCTTGTGGATGACAT
  TGGTCGACACAGGAGCGATAAGATCATCAACAACAGGCCTTGCCAGATCCTGAGGGGGAAGAGTTTCCTG
  TGGAAGAAATGGAAGAATCTATGTGTGGGGGACGTGGTGTGTCTCAGCAAAGACAGCATTGTCCCGGCCG
  ACTTGCTCCTGCTGGCCAGCACAGAGCCCAGTAGCCTGTGCTACGTGGAGACCGCAGACATCGACGGGGA
  GACCAACTTGAAGTTCAGGCAGGCTCTGACGGTCACGCACCATGAACTTACCAGCCCGAAGAAGATGGCT
  TCCTTCCAGGGTACAGTGACTTGCGAGGAACCCAACAGCCGAATGCATCATTTTGTGGGGAGTCTGGAGT
  GGAACAGCAGGAAGTACCCTTTGGACATCGGGAATCTGCTTCTGCGTGGCTGCAAGATCCGCAACACAGA
  CACCTGTTACGGCCTCGTCATCTATGCTGGTTTAGACACAAAGATCATGAAGAATTGTGGGAAGATTCAT
  CTGAAGAGGACCAAGCTGGACCTGATGATGAACAAGCTGGTGGCCCTGATCTTCCTGTCCCTGGTAATCG
  CGTCCCTGTTGCTGACGGTGGGCTTCACCTTCATGGTGAAACAGTTCAAAGCCAAGCATTACTACATGTC
  ACCCACCCACGGGCGCTCGGACGCGATGGAGTCCTTCTTCATCTTCTGGGGCTTCCTCATCCTGCTCAGC
  GTCATGGTGCCCATGGCCATGTTTATCATTGCGGAGTTCATCTACCTAGGCAACAGCATTTTCATTAACT
  GGGACCTCAATATGTACTACGAGCCACTGGACATGCCAGCCAAGGCCCGCAGCACCAGTCTCAACGACCA
  GCTGGGCCAGGTGCAGTACATCTTCTCAGACAAGACCGGGACGCTGACGCAGAACATCATGACCTTCAAG
  AAGTGCTGTATCAATGGCTGCATCTATGATTCAGACGATGAACATGGGACGCTTCGAAAGCGGAACCCAT
  ATGCCTGGAACCCATTCGCTGATGGCAAACTCCAGTTCTACAATAAGGAGCTGGAATCCCTGGTGCAAGG
  CCGGCAGGACCGAGCGGTTCAGGAGTTTTGGCGTCTGCTGGCCATCTGCCACACCGTGATGGTACAGGAG
  AAAGACAACCAGCTGCTGTACCAGGCTGCCTCCCCTGATGAGGAAGCACTGGTCACCGCTGCCCGGAACT
  TTGGCTACGTATTTCTGTCTCGGACCCAGGACACCATCACACTGGTGGAGCTGGGGGAGGAACGGGTCTA
  CCAGGTTCTGGCCATGATGGATTTTAACAGCGTTCGCAAACGGATGTCAGTGTTGGTCCGAAACCCAGAG
  GGCTCCATCTGCCTCTACACCAAGGGTGCCGACACTGTCATCCTGGAGCGTCTCCGCAGTAAGGGTGTCA
  TGGAGGCCACCACCGAGGAAGTCTTGGCTGCCTTTGCAGAGCAGACCCTGCGCACCCTGTGCCTGGCCTA
  CAAGGATGTGGAGGAGGATGCCTACAAAGAATGGGAGCCGGAGCACCAGGAGGCCGCCCTCCTGCTACAG
  AACCGTGCGCAGGCCCTGCACCAGGTGTATAATAAGATGGAACAGAACCTGCAGCTGCTGGGAGCCACCG
  CCATTGAAGACAAACTGCAAGATGGCGTTCCTGAGACCATCAAATGCCTCAAGAAGGGGAACATTAAAAT
  ATGGGTGCTGACCGGGGACAAGCCAGAGACCGCAGTGAACATCGGCTTTGCGTGCCAGCTGCTGTCGGAG
  AATATGATAATTCTGGAAGATAAGGACATCAATCAAGTACTGGAAAGGTATTGGGAAGACAACGTACACC
  AGAAGGCGTTCAAAATGATGACCCACCACAACATGGCCTTGGTTATCAATGGAGAATTCTTGGATCAGCT
  CCTCCTGTCCTTGCGCAAGGAGCCCCGAGCCCTAGTCCAGAATGCAGTGGTGGATGAGGTCGCCCAGGAG
  CCGGTTGTGTCCGCGTTAGACTTCCTCCAGAAGCGGAGGATCTCCCAGATGTGGAGAAACGCTGGCCCCT
  CTCTGGGAACGTCCCACTCAGCCGACTCCAAGATCCGGGAGAGCCCAGAGGTGCAGCGGGAGAGGGCCTT
  TGTAGACCTGGCCTCCAAATGCCAGGCTGTCATCTGCTGCAGGGTGACCCCCAAGCAAAAGGCCCTTGTG
  GTGGCATTGGTGAAGAAATACCAGCAGGTGGTGACGCTGGCCATCGGAGATGGAGCCAACGATGTGAACA
  TGATCAAGACTGCGGACATTGGTGTAGGACTGGCAGGCCAGGAGGGCATGCAGGCAGTTCAGAACAGCGA
  CTACGTGCTGGCCCAGTTCTGCTACCTGCAGCGGCTGCTCCTTGTGCATGGGCGCTGGTCCTACATGCGA
  GTCTGCAAGTTCCTGCGTTACTTCTTCTACAAGACAGTGGCCAGCATGATGGCCCAGATATGGTTCTCTC
  TAGTCAACGGCTTCTCTGCACAGCCCCTGTATGAAGGCTGGTTCCTGGCTCTCTTTAACCTGCTGTATTC
  CACGCTTCCAGTCCTGTATATTGGCCTGTTTGAGCAGGATGTGACCGCAGAGAAGAGCCTAAAGATGCCG
  GAGTTGTACATGGCCGGCCAGAAAGGCGAGCTGTTCAACTACAGCATTTTCATGCAAGCCATCACCCACG
  GCACCATCACATCCATGATCAACTTCTTTGTAACGGTTATGGTCAGCTCTGACATGTCCAAGGCTGGCAG
  CTCCCACGACTACCAGTCCTTAGGGGTATTGGTGGCCATATCTAGCCTACTGTCAGTCACCCTGGAGGTC
  ATGCTTGTGGTAAAGTACTGGACACTCCTGTTTGTGGGCGCAGTCGTGCTCAGCCTCAGCTCCTATGTCC
  TCATGACCAGCCTCACTCAGAGTCTATGGATGTACAGGATCTCGCCAAAGACCTTCCCATTTCTGTTTGC
  TGACTACAACGTGCTCTTCGAGCCTTGCAGTCTGCTGTTGATTGTACTCAATGTGGCCCTGAACGTTCTG
  CCCATGCTGGCTTTGCGCACAATCCATAGAACTGTCTTGAAACAGCGCCCTAAGGGGGAAGAGGAGGCCC
  CGAGTGAAGAAGTAGCCGTGGAGCCGGCCATGAGGCATCTTCGCCGTGGCATCCCAGCTCGCCGTTCCAG
  CTATGCCTTCTCCCACCGTGAGGGCTACGCAAACCTCATCACCCAGGGCACCATCCTGCGCAGGCAAACG
  CACGTGGATGATTCCGATGGGGGCACAGTCTGTGAATCCCTGAACCCACCCGAAGAAGACATTCCCTTGC
  AAAACAAAGATTCAGTATTTAATCCGCGGAAGATCTCCATCCTGGCCAAGAAGAGACGTCATTTCTTTGG
  AAAGGGGTCCCAGGAAGAGGTCCACCCCAACACTAGCTCCCAGACCATGGAGAAACAGCCCACCATTCAC
  AGAGACTCTGAGACCCAGAAGCTACCCACCACCACAAGTGCCACGTCGGGGAAGCTGCTGCCGTCCGCAT
  CCGAGGATGAGGCTTTCTACAGTGTGGCTTCACAGTACACTCTGGCAAGCCAGCCAAAACACACGGATGT
  CCACTCCTCATTCTGGAAGAGCCCCCTCTGGAGGGATTCAGCCTCTTCTAGTCCGAGCCAGCTGGAAGTC
  CCCAGAAAGCAAAGTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_026094
• Insert Size: 4008 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026094.3, NP_080370.2
• RefSeq Size: 4411 bp
• RefSeq ORF: 4008 bp
• Locus ID: 67331
• UniProt ID: Q6UQ17
• Cytogenetics: 10 C1
• Gene Summary: P4-ATPase flippase which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. May be responsible for the maintenance of asymmetric distribution of phosphatidylserine (PS) in spermatozoa membranes. Involved in acrosome reactions and binding of spermatozoa to zona pellucida.[UniProtKB/Swiss-Prot Function]