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Atp8b3 (NM_026094) Mouse Untagged Clone

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SKU
MC224261
Atp8b3 (untagged) - Mouse ATPase, class I, type 8B, member 3 (Atp8b3), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $1,828.00
7 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Atp8b3
Synonyms 1700042F02Rik; 1700056N23Rik; ATPIK; SAPLT
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC224261 representing NM_026094
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATGGAGTTCATCTCGGGGAGAACCTTGAGGATAAAGACACAGAGTTCACCTGGGAGGTGAAGGCCA
ATGACAGAACCTATCACAAGCAGTTCAAGAAGAAGGGCTTTCTATGCTGGAGGCAGAAGAAGTACAAGAG
CAACGCCATCCACACTGCCAAATACAACATCTTCTCCTTCCTGCCTCTGAACCTGTACGAGCAGTTCCAT
CGAATGTCCAATCTCTACTTCCTTTTTATCATCATCCTGCAGGGCATCCCCGAGATCTCCACACTGCCCT
GGTTCACGCTCTTTGCACCTCTGGTCTGCCTGTTTGTGATCCGGGCCACTCGTGATCTTGTGGATGACAT
TGGTCGACACAGGAGCGATAAGATCATCAACAACAGGCCTTGCCAGATCCTGAGGGGGAAGAGTTTCCTG
TGGAAGAAATGGAAGAATCTATGTGTGGGGGACGTGGTGTGTCTCAGCAAAGACAGCATTGTCCCGGCCG
ACTTGCTCCTGCTGGCCAGCACAGAGCCCAGTAGCCTGTGCTACGTGGAGACCGCAGACATCGACGGGGA
GACCAACTTGAAGTTCAGGCAGGCTCTGACGGTCACGCACCATGAACTTACCAGCCCGAAGAAGATGGCT
TCCTTCCAGGGTACAGTGACTTGCGAGGAACCCAACAGCCGAATGCATCATTTTGTGGGGAGTCTGGAGT
GGAACAGCAGGAAGTACCCTTTGGACATCGGGAATCTGCTTCTGCGTGGCTGCAAGATCCGCAACACAGA
CACCTGTTACGGCCTCGTCATCTATGCTGGTTTAGACACAAAGATCATGAAGAATTGTGGGAAGATTCAT
CTGAAGAGGACCAAGCTGGACCTGATGATGAACAAGCTGGTGGCCCTGATCTTCCTGTCCCTGGTAATCG
CGTCCCTGTTGCTGACGGTGGGCTTCACCTTCATGGTGAAACAGTTCAAAGCCAAGCATTACTACATGTC
ACCCACCCACGGGCGCTCGGACGCGATGGAGTCCTTCTTCATCTTCTGGGGCTTCCTCATCCTGCTCAGC
GTCATGGTGCCCATGGCCATGTTTATCATTGCGGAGTTCATCTACCTAGGCAACAGCATTTTCATTAACT
GGGACCTCAATATGTACTACGAGCCACTGGACATGCCAGCCAAGGCCCGCAGCACCAGTCTCAACGACCA
GCTGGGCCAGGTGCAGTACATCTTCTCAGACAAGACCGGGACGCTGACGCAGAACATCATGACCTTCAAG
AAGTGCTGTATCAATGGCTGCATCTATGATTCAGACGATGAACATGGGACGCTTCGAAAGCGGAACCCAT
ATGCCTGGAACCCATTCGCTGATGGCAAACTCCAGTTCTACAATAAGGAGCTGGAATCCCTGGTGCAAGG
CCGGCAGGACCGAGCGGTTCAGGAGTTTTGGCGTCTGCTGGCCATCTGCCACACCGTGATGGTACAGGAG
AAAGACAACCAGCTGCTGTACCAGGCTGCCTCCCCTGATGAGGAAGCACTGGTCACCGCTGCCCGGAACT
TTGGCTACGTATTTCTGTCTCGGACCCAGGACACCATCACACTGGTGGAGCTGGGGGAGGAACGGGTCTA
CCAGGTTCTGGCCATGATGGATTTTAACAGCGTTCGCAAACGGATGTCAGTGTTGGTCCGAAACCCAGAG
GGCTCCATCTGCCTCTACACCAAGGGTGCCGACACTGTCATCCTGGAGCGTCTCCGCAGTAAGGGTGTCA
TGGAGGCCACCACCGAGGAAGTCTTGGCTGCCTTTGCAGAGCAGACCCTGCGCACCCTGTGCCTGGCCTA
CAAGGATGTGGAGGAGGATGCCTACAAAGAATGGGAGCCGGAGCACCAGGAGGCCGCCCTCCTGCTACAG
AACCGTGCGCAGGCCCTGCACCAGGTGTATAATAAGATGGAACAGAACCTGCAGCTGCTGGGAGCCACCG
CCATTGAAGACAAACTGCAAGATGGCGTTCCTGAGACCATCAAATGCCTCAAGAAGGGGAACATTAAAAT
ATGGGTGCTGACCGGGGACAAGCCAGAGACCGCAGTGAACATCGGCTTTGCGTGCCAGCTGCTGTCGGAG
AATATGATAATTCTGGAAGATAAGGACATCAATCAAGTACTGGAAAGGTATTGGGAAGACAACGTACACC
AGAAGGCGTTCAAAATGATGACCCACCACAACATGGCCTTGGTTATCAATGGAGAATTCTTGGATCAGCT
CCTCCTGTCCTTGCGCAAGGAGCCCCGAGCCCTAGTCCAGAATGCAGTGGTGGATGAGGTCGCCCAGGAG
CCGGTTGTGTCCGCGTTAGACTTCCTCCAGAAGCGGAGGATCTCCCAGATGTGGAGAAACGCTGGCCCCT
CTCTGGGAACGTCCCACTCAGCCGACTCCAAGATCCGGGAGAGCCCAGAGGTGCAGCGGGAGAGGGCCTT
TGTAGACCTGGCCTCCAAATGCCAGGCTGTCATCTGCTGCAGGGTGACCCCCAAGCAAAAGGCCCTTGTG
GTGGCATTGGTGAAGAAATACCAGCAGGTGGTGACGCTGGCCATCGGAGATGGAGCCAACGATGTGAACA
TGATCAAGACTGCGGACATTGGTGTAGGACTGGCAGGCCAGGAGGGCATGCAGGCAGTTCAGAACAGCGA
CTACGTGCTGGCCCAGTTCTGCTACCTGCAGCGGCTGCTCCTTGTGCATGGGCGCTGGTCCTACATGCGA
GTCTGCAAGTTCCTGCGTTACTTCTTCTACAAGACAGTGGCCAGCATGATGGCCCAGATATGGTTCTCTC
TAGTCAACGGCTTCTCTGCACAGCCCCTGTATGAAGGCTGGTTCCTGGCTCTCTTTAACCTGCTGTATTC
CACGCTTCCAGTCCTGTATATTGGCCTGTTTGAGCAGGATGTGACCGCAGAGAAGAGCCTAAAGATGCCG
GAGTTGTACATGGCCGGCCAGAAAGGCGAGCTGTTCAACTACAGCATTTTCATGCAAGCCATCACCCACG
GCACCATCACATCCATGATCAACTTCTTTGTAACGGTTATGGTCAGCTCTGACATGTCCAAGGCTGGCAG
CTCCCACGACTACCAGTCCTTAGGGGTATTGGTGGCCATATCTAGCCTACTGTCAGTCACCCTGGAGGTC
ATGCTTGTGGTAAAGTACTGGACACTCCTGTTTGTGGGCGCAGTCGTGCTCAGCCTCAGCTCCTATGTCC
TCATGACCAGCCTCACTCAGAGTCTATGGATGTACAGGATCTCGCCAAAGACCTTCCCATTTCTGTTTGC
TGACTACAACGTGCTCTTCGAGCCTTGCAGTCTGCTGTTGATTGTACTCAATGTGGCCCTGAACGTTCTG
CCCATGCTGGCTTTGCGCACAATCCATAGAACTGTCTTGAAACAGCGCCCTAAGGGGGAAGAGGAGGCCC
CGAGTGAAGAAGTAGCCGTGGAGCCGGCCATGAGGCATCTTCGCCGTGGCATCCCAGCTCGCCGTTCCAG
CTATGCCTTCTCCCACCGTGAGGGCTACGCAAACCTCATCACCCAGGGCACCATCCTGCGCAGGCAAACG
CACGTGGATGATTCCGATGGGGGCACAGTCTGTGAATCCCTGAACCCACCCGAAGAAGACATTCCCTTGC
AAAACAAAGATTCAGTATTTAATCCGCGGAAGATCTCCATCCTGGCCAAGAAGAGACGTCATTTCTTTGG
AAAGGGGTCCCAGGAAGAGGTCCACCCCAACACTAGCTCCCAGACCATGGAGAAACAGCCCACCATTCAC
AGAGACTCTGAGACCCAGAAGCTACCCACCACCACAAGTGCCACGTCGGGGAAGCTGCTGCCGTCCGCAT
CCGAGGATGAGGCTTTCTACAGTGTGGCTTCACAGTACACTCTGGCAAGCCAGCCAAAACACACGGATGT
CCACTCCTCATTCTGGAAGAGCCCCCTCTGGAGGGATTCAGCCTCTTCTAGTCCGAGCCAGCTGGAAGTC
CCCAGAAAGCAAAGTTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_026094
Insert Size 4008 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026094.3, NP_080370.2
RefSeq Size 4411 bp
RefSeq ORF 4008 bp
Locus ID 67331
UniProt ID Q6UQ17
Cytogenetics 10 C1
Summary P4-ATPase flippase which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. May be responsible for the maintenance of asymmetric distribution of phosphatidylserine (PS) in spermatozoa membranes. Involved in acrosome reactions and binding of spermatozoa to zona pellucida.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_026094" in other vectors (4)
SKU Description Size Price
MG221416 Atp8b3 (tGFP-tagged) - Mouse ATPase class I type 8B member 3 (Atp8b3), (10ug) 10 ug
$1,914.00
MR221416 Atp8b3 (Myc-DDK-tagged) - Mouse ATPase, class I, type 8B, member 3 (Atp8b3) 10 ug
$1,714.00
MR221416L3 Lenti ORF clone of Atp8b3 (Myc-DDK-tagged) - Mouse ATPase, class I, type 8B, member 3 (Atp8b3) 10 ug
$2,014.00
MR221416L4 Lenti ORF clone of Atp8b3 (mGFP-tagged) - Mouse ATPase, class I, type 8B, member 3 (Atp8b3) 10 ug
$2,014.00

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