Aox1 (NM_009676) Mouse Untagged Clone

CAT#: MC224256

Aox1 (untagged) - Mouse aldehyde oxidase 1 (Aox1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Aox1
• Synonyms: AI196512; AI255253; Ao; Aox-1; Aox-2; Aox2; Moro; Ro
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224256 representing NM_009676
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCCCATTCAGCTGCTCTTCTACGTGAATGGCCAGAAGGTGGTAGAAAAAAATGTCGATCCTGAAA
  TGATGCTTTTACCATACCTGAGGAAGAATCTCCGACTCACAGGAACTAAGTATGGCTGTGGAGGCGGGGG
  CTGTGGGGCCTGCACAGTGATGATCTCGCGGTACAACCCCAGCACCAAGGCGATCAGGCATCATCCTGTC
  AATGCCTGTCTGACCCCCATCTGCTCTCTACATGGTACAGCAGTCACCACGGTAGAAGGCTTAGGCAACA
  CCAGGACCAGGCTTCATCCTATTCAGGAGAGAATTGCCAAGTGTCACGGCACCCAGTGTGGATTCTGTAC
  TCCTGGGATGGTGATGTCCATGTACGCACTGCTCAGGAACCATCCAGAGCCCACTCTAGATCAGTTAACT
  GATGCCCTTGGTGGGAATCTGTGCCGCTGCACTGGATATAGGCCCATAATTGATGCCTGCAAGACTTTCT
  GTAAAGCCTCTGGCTGCTGTCAAAGTAAAGAAAATGGGGTGTGCTGTTTGGATCAAGAAATAAATGGATT
  GGCAGAATCCCAGGAAGAAGATAAGACAAGTCCAGAACTGTTCTCAGAAGAGGAATTTCTGCCACTGGAC
  CCGACCCAAGAGCTGATATTTCCTCCCGAGCTAATGAGAATAGCTGAGAAACAGCCACCAAAGACCAGAG
  TGTTTTATGGTGAGAGGGTGACATGGATTTCCCCCGTGACTCTGAAGGAACTTGTGGAAGCTAAATTCAA
  GTATCCCCAGGCCCCTATTGTCATGGGGTACACTTCTGTGGGACCTGAAGTAAAGTTTAAAGGTGTCTTC
  CACCCCATCATAATTTCTCCTGACAGAATTGAAGAGCTGGGTGTCATAAGCCAGGCCAGGGATGGGCTGA
  CCCTGGGTGCTGGCCTCAGCCTGGATCAGGTGAAGGACATTCTGGCTGATATAGTCCAGAAGCTTCCAGA
  AGAGAAGACACAGACATACCGTGCTCTCCTGAAGCACCTGAGAACTCTGGCTGGCTCCCAGATCAGGAAC
  ATGGCTTCTCTAGGGGGCCACATTGTGAGCAGACATCTGGACTCAGATCTGAATCCCCTTCTGGCTGTGG
  GTAACTGTACCCTCAACTTACTGTCCAAAGATGGAGAACGGCGGATCCCTTTAAGTGAAGAGTTTCTCCG
  AAAGTGTCCTGAAGCAGATCTTAAGCCTCAGGAAGTCTTGGTCTCAGTGAACATCCCCTGGTCCAGGAAG
  TGGGAGTTTGTGTCAGCCTTCCGTCAAGCGCAAAGACAACAGAATGCACTAGCAATAGTCAACTCCGGAA
  TGAGAGTCCTTTTTAGAGAAGGAGGTGGCGTCATTGAAGAGTTATCCATTTTGTATGGAGGTGTCGGTTC
  AACTATCATCAGTGCCAAGAACTCCTGTCAGAGACTCATTGGGAGGCCCTGGAATGAAGGGATGCTGGAC
  ACAGCCTGTAGGCTGGTTTTGGATGAAGTCACCCTTGCAGCCTCAGCTCCTGGTGGGAAGGTGGAGTTCA
  AGAGGACCCTCATCATCAGCTTCCTTTTCAAGTTCTACCTGGAGGTGTCACAGGGTTTGAAGAGGGAGGA
  CCCAGGTCACTCTCCTAGCCTGGCAGGCAACCATGAGAGTGCTTTAGATGATCTTCATTCAAAACATCCC
  TGGAGAACATTAACCCACCAGAATGTAGATCCAGCACAGCTGCCTCAGGACCCCATTGGACGTCCCATCA
  TGCACCTTTCTGGGATTAAACATGCCACGGGCGAGGCCATCTACTGTGACGACATGCCTGCAGTAGACCG
  GGAGCTTTTCCTCACTTTTGTAACAAGTTCAAGAGCACACGCTAAGATTGTGTCCATTGATCTGTCGGAA
  GCTCTCAGCCTGCCTGGTGTGGTGGACATCATTACTGCAGATCATCTTCAGGAAGCAAACACCTTCGGCA
  CAGAGACATTTCTGGCCACAGATGAGGTACACTGCGTGGGCCATCTTGTCTGTGCTGTGATTGCAGATTC
  TGAGACACGGGCAAAGCAAGCGGCGAAGCAAGTGAAGGTGGTCTACCAAGACTTGGCGCCTCTGATCCTA
  ACGATTGAGGAAGCTATACAACACAAGTCCTTCTTCAAGTCAGAACGGAAGCTGGAGTGTGGGAATGTTG
  ACGAAGCATTTAAAATCGTTGATCAAATTCTTGAAGGTGAAATACACATAGGCGGCCAGGAACATTTTTA
  TATGGAAACCCAAAGCATGCTTGTTGTTCCCAAAGGAGAGGATGGAGAGATTGACATCTATGTGTCTACA
  CAGTTTCCCAAATATATACAGGATATAGTCGCTGCAACCTTGAAGCTCTCAGCCAACAAGGTCATGTGTC
  ATGTAAGGCGTGTTGGCGGGGCATTTGGAGGGAAGGTGGGCAAGACCAGCATCTTGGCAGCCATCACTGC
  ATTTGCTGCTAGCAAACACGGTCGCGCAGTCCGCTGCATTCTGGAACGAGGAGAAGACATGTTAATAACT
  GGAGGCCGCCATCCTTACCTTGGAAAGTATAAAGCTGGATTCATGAATGACGGCAGAATCTTGGCCCTGG
  ACGTGGAGCACTACTGCAATGGAGGGTGCTCCCTGGATGAGTCACTATGGGTGATAGAAATGGGGCTTCT
  GAAGCTGGACAACGCTTACAAGTTTCCCAACCTACGCTGCCGGGGCTGGGCCTGCAGAACCAACCTTCCA
  TCCAACACTGCTCTGCGTGGGTTTGGCTTTCCTCAGGCAGGGCTGGTCACCGAAGCCTGTATCACAGAAG
  TGGCAATCAAATGTGGCCTGTCCCCTGAGCAGGTTCGAACCATAAATATGTACAAGCACGTTGATACTAC
  CCATTACAAGCAAGAGTTCAGCGCCAAGGCCCTCTCTGAGTGCTGGAGAGAGTGCATGGCCAAGTGTTCC
  TACTTTGAGAGGAAAGCAGCCATAGGAAAATTCAACGCAGAGAATTCCTGGAAGAAGAGAGGAATGGCTG
  TGATTCCCCTGAAGTTTCCTGTGGGTATTGGATCAGTAGCCATGGGACAGGCAGCTGCCTTGGTTCATAT
  TTATTTGGATGGCTCTGCACTGGTCTCTCATGGTGGAATTGAGATGGGGCAGGGTGTGCACACTAAAATG
  ATTCAGGTGGTCAGCCGGGAACTAAGGATGCCGATGTCCAGTGTCCACCTGCGTGGGACAAGCACAGAAA
  CCGTCCCCAACACAAATGCCTCTGGAGGCTCTGTGGTGGCAGATCTCAATGGACTGGCAGTAAAGGATGC
  CTGTCAGACCCTTCTAAAACGCCTTGAACCCATCATCAGCAAGAATCCTCAGGGAACTTGGAAGGATTGG
  GCCCAGACTGCTTTTGACCAAAGCATCAGTCTCTCGGCTGTTGGATATTTCAGGGGTTATGAGTCGAATA
  TAGACTGGGAGAAAGGGGAAGGTCATCCCTTCGAATACTTTGTGTTTGGAGCTGCCTGCTCAGAGGTTGA
  AATAGACTGCCTGACTGGGGACCATAAGAATATCAGAACAAACATCGTGATGGATGTTGGCCACAGCATA
  AACCCAGCCCTTGACATAGGTCAGGTTGAAGGTGCATTTATTCAAGGAATGGGACTTTACACAATAGAGG
  AGCTGAGTTACTCTCCTCAGGGCACTCTATACAGTCGTGGTCCAAACCAATACAAGATTCCTGCCATCTG
  TGACATCCCCACGGAAATGCACATTTCTTTTTTGCCCCCATCTGAACACTCAAACACCCTGTATTCATCT
  AAGGGCCTGGGAGAGTCTGGGGTGTTTCTGGGATGTTCGGTATTTTTTGCCATCCATGATGCAGTGAAGG
  CAGCGCGGCAGGAGAGAGGCATCTCTGGACCATGGAAACTCAACAGTCCTCTGACTCCAGAGAAAATCAG
  AATGGCCTGTGAAGATAAGTTCACCAAAATGATCCCAAGAGATGAGCCTGGATCCTATGTTCCCTGGAAC
  ATACCTGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009676
• Insert Size: 4002 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009676.2, NP_033806.2
• RefSeq Size: 4382 bp
• RefSeq ORF: 4002 bp
• Locus ID: 11761
• UniProt ID: O54754
• Cytogenetics: 1 28.86 cM
• Gene Summary: Oxidase with broad substrate specificity, oxidizing aromatic azaheterocycles, such as N1-methylnicotinamide, N-methylphthalazinium and phthalazine, as well as aldehydes, such as benzaldehyde, retinal, pyridoxal, and vanillin. Plays a role in the metabolism of xenobiotics and drugs containing aromatic azaheterocyclic substituents. Participates in the bioactivation of prodrugs such as famciclovir, catalyzing the oxidation step from 6-deoxypenciclovir to penciclovir, which is a potent antiviral agent. Also plays a role in the reductive metabolism of the xenobiotic imidacloprid (IMI) via its nitroreduction to nitrosoguanidine (IMI-NNO) and aminoguanidine (IMI-NNH(2)). Is probably involved in the regulation of reactive oxygen species homeostasis. May be a prominent source of superoxide generation via the one-electron reduction of molecular oxygen. Also may catalyze nitric oxide (NO) production via the reduction of nitrite to NO with NADH or aldehyde as electron donor. May play a role in adipogenesis. Cannot use xanthine and hypoxanthine as substrate.[UniProtKB/Swiss-Prot Function]