Jcad (NM_001081963) Mouse Untagged Clone

CAT#: MC224250

9430020K01Rik (untagged) - Mouse RIKEN cDNA 9430020K01 gene (9430020K01Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Jcad
• Synonyms: 1110056P05Rik; Gm328; Jcad; mKIAA1462
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224250 representing NM_001081963
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATAGTGTGGAAGATCTCCTGATTTCTCATGGATACAAACCAGCCCGAGATGCCGCAGCACCATGTG
  AGGACAAATCAGAGAGATGCAGGTCGACAAGGACAGGACCCCGAGCTGGCCAAGGCCTATTGAATGGGTA
  CAAGGATGGCGCTACAGCCCATACGCACAGTAGGACATCCCTGGGGACAGGACACGTGAGCAACTCTGAA
  AACCGCATCAGCAGGCCAAGAGGCCACAGGGAGCACCAAAGCACTTCTAGAACTCCTGAGGCACGGTTTT
  TAAATCAACCCTCCTTAGCATGGTCCTCTCAGCCCCAAAGTGGTAGAGACGACATCTACTGGAGCAGAGG
  AAGACAGGAGGGCAGTGGCTCCCTGTGTCCCAGGGACTGGAAAGAACTGGAAAGCAGAGGAATGGCTCAG
  GCTTACAGTCTGCCCGTCCATGTGAGAGAGAATCTGTGGGAAGTTGCAGGAAGGACAGAGCATGTGATGA
  AGAATGCCATCTGGGAAGAAGAGCTGAGAATGCAGGACATGAGCTTGGAAAGCTGGAAGAAGCCAAGGGA
  GTTAGGGAGGCAGGCATCCGATGGGGATGGACGGAAAAGGCCCCAGGAAAAGTTCGAGGGTCTGTACCCA
  TTTGTTCATGGGGAGCACACGTCTCAGAACAGAAAAAAATCCCAGTCATTGCCCCGAGCTCTTTCTCCTA
  AGAGCCTGAATTTCACAGAAATTCCTGTTCCACTACATGATGGTCACATAACAGGTGTCCCAAAAGTGCC
  ACCATATCCTCCTAGCTTCCCATCCCCTTCGGAACCCATGAGGAACCTTGAGAAGGCTAGCTCCTCAGGC
  CCTTTTCCCAGGCCTAAGTTTGGGAAACCCCTTAAGACTCCGTGTTATAGCTCTCACTCACAGCCCAGGG
  GAGAAGGTGGATTTCAGGACCACCAGCACAGGGACCCACGTGGCTCCTACCCAACAAGAAGTAAGGATCC
  CAGCCATGAGTTGGGTATGCTGGATCCTGGCTTGGAGCCCCCAGTGTATGTGCCTCCACCTTCATATAGG
  TCACCCCCTCAGCACATTCCAAACCCATACCTTGAAGATCCTGTGCCCAGGCATGTGAGCAGCAACCAGA
  GTCAGCAGCAAGTACCTGAGAAACCTGAGACCAGCTGTCCACTTCCTTCTGGCTCTCTTGCAGCTAGGGA
  TCTCTATGATGCAATGCCTGGCTCTCCTCCGCAAGGTCTTCCTCCACAACCCTATCCTATTGCCACCCAT
  GGGGGTTCTATTCAGTACATTCCATTTGATGATCCACGGATCCGACATATCAAACTAGCTCAGCCCCCAG
  AATTCTATGAAGAGGCAAAGCTTGATGATACATCCTATAACCCTGGTTTACTCACTACCCAAGAGCCAGC
  CATTGGGAAAAGACAGTACGATGATGCCCCTTCGGTACCACGGGGCCCAACGCCTTCACCAGTCAATGAG
  CAGAGCTCTGCCTTTGTCCATTCCAGTCCCCGGTGGCTGCAGGGCCAGCTCCCCCTGGGCATTGGACCTG
  GAGGCTTCCATGGCCAAACAGAGCATCATGTCATGGGCGGATTAACAACTAATGTGACAGACATCAAGGC
  AGAAGGCCATGCCTCTTCACCACAGCCACAGAGTGAGGGTACCTGCAAAACCTACACTAAGCTCAGAAAG
  TTTGAAACTGGGGTTCAGAGCAAGAAAAGTTCAAAGAAGAAAAGTAACGCAACTATATTTTGTTTGGTCT
  CCATCCCAGTTAAATCTGAGTCACTTGTGCTAGCTACGGATACAAACAACAATGACTTTAAGCTGGTTGC
  TGATAAGACCCGAGGGCTGTGCCAGGGTTCAGCCCTGCAGGAGCAGAGTCTGCTGAGTATGTCTTCCACT
  GACCTGGAGCTGCAAGCCCTCATGGGAAGCATGGCTTGGAGAAGAACATCTCCAAGGCAAGGTCTGAGGG
  AGTCAGAAGATGGCCAAATTGATGACCCCAGAATCCTCCATCTCATCAAACCCAAAGAACTTCAGGCTTC
  CAGCCCTTGGCCAGGACACCAATACAGAGATCAACAGACCCAAACCAGTTTCCATGAAGACTCCAAAAGC
  TCACAGCTCCTCCCTGCCACAAAGCCAGGAGAGGCCAGCAATGTAGCTCCGACCCCAACATGCCCAGATA
  CCACTGCCTCCGAAGTATGCCTCCATACAGCCTTAGCATTCAGTGATCAAAATCAGAAGCCCAGTGTACC
  TCACCTCCAAGGACAAACGTCCCTTAGCCCGTCTCGAAACAGTGCTTTCTCAAGGACTTCCTCAGCCATA
  AACCAGGCATCTATGTCCAAAGGGACCTCTGACCAGCTCCCTGGTGCCAACCCTGTTCCCAAGCCAGAGG
  TGGTGAAGGGGGAGTCCACAACAGGCCAGTGCAATAGCACACAACTCTTCGGTCAGTTTCTCTTGAAACC
  AGTTAGCCGGCGGCCCTGGGATTTGATAAGTCAGTTAGAAAGTTTTAACAAGGAACTTCAGGAAGAGGAA
  GAAAGCCATGGTGGTAGTGGTAGTGAGGACAGTGAGGCAGAACAGCCAGAGGACTGTGCAGATTCCAGAA
  CCAAGTCCTGGGCTCTCCAGGGAACTAGAACAGCACAGCAGCCTGCAGGGCTGGCGCTGGAGAATGTAGC
  TTCCCCAGATAGAAGACTTAATGACTCACAGAGCTGGAATGAAGAACCAAAGCCTGGCCACTCAAGTGTC
  CATCCACAGTCCCTGGGCCCATCACAGGAGGAAGGCAGCAGAGGTGTTCCAGTCCAATGGGCAGATGGAA
  GCCTGACTGCAGAGCAGAAAAGCCAGGAGGATTTGAATGGGATGTGTGAGCGAGACTTTAGCCCAAGGCC
  TGTGAGCAGGATTGCACCCATTGACACAAAAGCAGCCCCTTTATACTGTCTGTCAGAACCAAGAGGAAGT
  CAAGAACTCACCAAATTCGGCGATGCTGTAGGGTCTGTGCAGCTGGGCAGAGAGACTCCCACACAGGTGG
  GTAATGGTGGGGACACAGAGGTCCTACCCTGTGTCCTTCTGCCACTGGCTGACAAATATCGAGGCCACTC
  AACACCAGACTTTCGGTCTTTAGAGCTCACACTGGGACAAGAACAGAATGCCTATAAATTAGAGTGTCTG
  GATTTAGAGAACACCGTGGAAGTCCTTCCAAGTGAGTCTCTGCAGGAAAGGGCAGAGAGGATCCTGGGCA
  TAGAGGTGGCCGTGGAGTCCCTTCTGCCAAGTGCCAGGAGAACAGAACAAAGCCAGCTTCCTGAGCCTGA
  TGCAAGTGCCTGCAACCCAAGTTCATCCAGAGAGGACTCATCACACAGCTTGGCACTACCAGTGGGGCCC
  AAAGTGGCCACTGACGCCTTCTATGGCAGGAGGAAGTGTGGCTGGACTGAGAGTCCTCTTTTTGTAGGAG
  AAAGGGCCCCCCAGGCTTCTATATGCTCAGATGTGGATGGCTTCCCTACAAGCCAGGCCACCAGTCCTGA
  GCCTGGGAAAAAGGATGAGGAGGCGAAAGCACCCTTCAAGTCCACTCTGTTCCATTTCATGGAAAAGTCC
  ACAAATGTGGTGGGTCCTGAAAAGAGGCTCAGAAACCCTTCCAAAGTGGTTGAGAACTTACAAGAAAAAC
  TGGTATCACCCCCAAAAAAGGCAGACTCTGTTCACTTGATAAGAATGAGGGAGGTCAACTCCTTGTCTCA
  GATGAGGTGTCTGAGCTCCAAGAGCGCTGACTCCGTGGAGGAGCCTGACCCCTTAAAGGTCATCAAAAGT
  TCAGCCTGGCTTTCAGAAGGCCTTACTTCCCTGGGTGGTAAAGACGAAGCCTGGCAAGCAGGGCACCTGC
  CCTCTGTCTCTCAGAATGAAAACGGACACCCCGAAGTGCCGAGGGACAAGATGTCAGACCAAGACTTGTG
  GTGTGCAGATTCCTATGATCCAAGCCGAGTGGAGAGGGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081963
• Insert Size: 3963 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081963.1, NP_001075432.1
• RefSeq Size: 6748 bp
• RefSeq ORF: 3963 bp
• Locus ID: 240185
• UniProt ID: Q5DTX6
• Cytogenetics: 18 A1