Ttbk2 (NM_080788) Mouse Untagged Clone

CAT#: MC224225

Ttbk2 (untagged) - Mouse tau tubulin kinase 2 (Ttbk2), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ttbk2
• Synonyms: 2610507N02Rik; AI326283; B930008N24Rik; mKIAA0847; Ttbk; Ttbk1; TTK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224225 representing NM_080788
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACTAGCCAACCGACGCCACAGGCTGGGAACGTGCTCTCCAATCAGCACCCGCGGCGGAGAGAATT
  GTAAAACCGGTAAGATCAAGTGCCTATTGAAAGAGACTCACTCTTGGCTAAGAAGAGAAATAGGAAATAG
  GTTAAATGGAATCCACCCTTGGGAGCTAGATGCCTGTGTAGCTGTTTTACCTCATCAGGGTTTTGCAATG
  AGTGGAGGAGGAGAGCAGCCAGATATCCTCAGTGTTGGAATCCTGGTCAAAGAAAGATGGAAAGTGTTAA
  GAAAGATTGGAGGTGGGGGCTTTGGAGAAATTTACGATGCCTTGGACATGCTCACCAGGGAGAATGTGGC
  GCTGAAGGTGGAGTCAGCTCAGCAGCCAAAGCAGGTTCTGAAGATGGAGGTTGCTGTGTTGAAGAAACTG
  CAAGGGAAAGACCATGTTTGTAGATTTATTGGCTGTGGGAGAAATGATCGTTTCAACTACGTGGTCATGC
  AATTGCAGGGACGGAATCTGGCAGATCTCCGCCGTAGCCAATCCCGGGGCACATTCACTATTAGCACTAC
  CCTTCGTCTTGGGAAACAGATTCTGGAGTCTATTGAAAGCATACATTCTGTGGGATTCCTTCACAGAGAC
  ATCAAACCGTCAAACTTCGCCATGGGACGTTTCCCCAGTACATGTAGGAAATGTTTCATGCTTGATTTTG
  GCTTGGCTCGACAATTTACTAATTCCTGTGGTGACGTCAGACCACCTCGTGCTGTGGCAGGCTTTCGAGG
  GACAGTTCGTTATGCATCAATCAATGCTCATCGGAACAGGGAAATGGGAAGACATGATGACCTTTGGTCT
  TTATTCTACATGTTGGTGGAGTTTGTGGTTGGCCAACTGCCTTGGAGAAAAATAAAGGACAAGGAGCAAG
  TAGGCTCCATTAAGGAGAGATATGACCACAGGCTCATGTTAAAACACCTCCCTCCAGAATTCAGCACCTT
  TCTTGACCATATTTCCTCTTTGGATTATTTTACAAAACCGGACTACCAGCTTCTAACATCCGTGTTTGAC
  AATAGCATCAAGACCTTTGGAGTAATTGAGAGTGACCCGTTTGACTGGGAGAAGAGTGGAACTGATGGCT
  CCCTGACAACCACCACCACCTCTGCCACCCCTCAGCTGCACACCCGCCTGACCCCTGCTGCTATCGGAAT
  TGCAAATGCCACCCCCATCCCAGGAGACTTGCTTCGAGAAAATACAGATGAAGTGTTTCCAGATGAACAG
  CTTAGTGATGGGGAGAACGGAATCCCTGTTGGTGTATCACCAGATAAATTGCCTGGATCTCTGGGGCACC
  CACGCCCTCAGGAAAAGGATGTCTGGGAAGAGATGGATATCAACAAGAACAAGATAAAGCTGGGAATTTG
  CAAAGCAGCTACTGAAGAAGAAAATAGCCATGGTCAAGTAAATGGCATACTCAATGCTCCAAGCCTTGGT
  TCACCAATTCGTGTCCGATCAGAGATTACTCAGCCAGACAGAGATGTTCCGTTAGTAAGGAAGCTACGTT
  CTATTCACAGCTTTGAGCTGGAAAAACGTTTGACACTTGAGCCAAAGCCAGATACTGACAAGTTTCTGGA
  GACCTGCATGGAGAAAATGCAGAAAGATTCCAGTGCAGGAAAAGAACCTGTCCCCCCTGCTCTGCCTCAT
  AAGCCGTGTGTCCCCGTCGTGACCCACACTGACCACATCTGGCACTATGATGACGAATATCTTCCTGATG
  CCTCCAAGCCTGCCTCTGCCAACACCCCGGAGCAGGCAGATGGTGGTGGCAGCAACGGATTTATAGCTGT
  TAACTTAAGCTCTTGCAAACAGGAGGTTGATTCCAAAGAATGGGTGATTGTGGACAAGGAGCAAGACCTT
  CAGGACTTTAGGACAAATGAGGTGTTAGGCCATAAGACAACTGGAAGCCCTTCAGATGAGGAGCCTGAAG
  TGCTTCAGGTCCTTGAGGGATCACCTCAAGATGAAAAGATCCAAGTAGGTCCTTGGACTGACAACCATCA
  CTTAAAGAAGGAAAGCTCAGGTGTGGTTTTAGCACTTTCTGCTGAATGCCCTGCTACTGCTGCTTCAGAA
  CTGTACACAGATAGGCTAGACCTCCAGGCTGGAGCTGCAAGTCAGTTCATCACAGTGACTCCCACAAGTC
  CCATGGAGGCACAAGCAGAAGGACCCCTGACTGCGATTACAATTCCTAGACCTTCTGTGGCATCAACACA
  GTCAACTTCAGGAAGCTTTCACTATGGCCCACAACCAGAGAAGAAAGATCTTCAGCCCTTGGAGCCCACT
  GTAGAACTGTACTCTCCAAGGGAGAACTTCTCCGGCTTGGTTGTGACAGAGGGTGAACTAGCTAGCGGAG
  GAAGCAGAGTGGATTTGGGACTTCAGATAGATCACACTGGTCATGACATGTTACCCAACATGAGAGATGG
  TGACACATCTCAAGACTTGGGACCAAAAGACCCTCCTGACCATAATAGATTAGCTGTGAAAGAATTTGAA
  CATCTCCCTGGAGAAACAGAAGAGAGAAGCCTTCTTCTGGGCTCAGAGAATGAAGATGAGAGGTTAAGTA
  AAGGGCAGCACTGTATTGAAGTCTCTTCCCCAGGAGAGTTAGTGACTGCAGAGAGGGCTCAGTTAGCTGC
  CACAGAACCTCTGCATGTATCAGAGACACAGAACTGTAGTGTGCTACCAAATCAGGACAAAACCCATGAG
  ATAATGAAGCTTTTGGCAGTTGGAACTTCAGAAATTTCTCCTCAAGCCATTGACCCACATGCTGAAGGGC
  AGATAGGCCAGATGGCAGCAATGCAGAAAAATAAGCTATTTAAAGATGATGGTATTCAGAGTGAAAGCTT
  GCCAAGGCAGCAGGGAGACCTCTCTGCTTTTTTGCACCAAGAGGGTAAGAGAGAGAAAGTTGTCCCTAGA
  AATGGAGAGCTCTATCATTGTGTCTCAGAGAATGAGCATGGTCCTCCTACTCGGAAGGACATGCTCCGGT
  CATCCTTTGTGACCAGGCACAGCCGGATCCCTGTTTTAGCACAAGAAATAGACTCAACTTTTGAATCATC
  CTCTGCTATTTCTGCAAAAGAAAAGCTTCTACAGAAGAAAGCCTATCAGCCAGAAATAGTCAAACTTCTT
  GTAGAAAAAAGGCAGTTCAAGTCTTTCCTGGGAGACCTCTCAAGTGCCTCTGATAAGCTGATAGAGGAGA
  AACTAGCCGCTGTTCCAGTCCCTTTTTCTGAGGAGGAAGTCTTCGCTCCCTTTTCTAGACTGGCAGCAGA
  TTCCCACCTGAGTAGATCAGTTGAAGATAGCTTTCTGTCACCCATCATCTCCCAGGCTAGAAAGAGCAAG
  ATTCCAAGGCCAGTATCCTGGGTCAGCACAGATCAAATTAATGGCTCCGCTTCACCTCAGTTCTTGCCTC
  GGCCACCACCAGGAAAGCCACCAGTCAGGCCTGGAGTAGAAGCCAGGCTACGCAGATATAAAGTTCTAGG
  GAGTAGTAACTCTGACTCAGACCTTTTCTCTCGCCTGGCCCAAATTCTTCAAAATGGATCTCAGAAATCC
  CGGAGTACTACCCAATGCAAGAGCCCAGGATCTCCTCACAATCCAAAAACACCACCCAAGAGTCCAGTTG
  TACCTCGAAGGAGTCCCAGTGCCTCTCCTCGAAGCTCATCCTTGCCTCGAACATCTAGTTCCTCACCATC
  TAGGGCTGGACGGCCCCACCATGACCAGAGGAGTTCTTCCCCACATCTGGGGAGAAGCAAGTCACCCCCT
  AGCCACTCAGGATCATCGTCCTCCAGGAGGTCCTGCCAACAGGAGCATTGCAAACCCAGCAAGAATGGCC
  CAAAAGGATCTGGCAGCCTCCACCACCACTCAACCAGCTCTAAAACTCCCCCAGGGAAGAGTAAGCCAGC
  CAGTAAACTCAGCAGATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_080788
• Insert Size: 3939 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080788.3, NP_542966.2
• RefSeq Size: 10906 bp
• RefSeq ORF: 3939 bp
• Locus ID: 140810
• UniProt ID: Q3UVR3
• Cytogenetics: 2 E5
• Gene Summary: Serine/threonine kinase that acts as a key regulator of ciliogenesis: controls the initiation of ciliogenesis by binding to the distal end of the basal body and promoting the removal of CCP110, which caps the mother centriole, leading to the recruitment of IFT proteins, which build the ciliary axoneme. Has some substrate preference for proteins that are already phosphorylated on a Tyr residue at the +2 position relative to the phosphorylation site. Able to phosphorylate tau on serines in vitro.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1).