Ttc21a (NM_028735) Mouse Untagged Clone

CAT#: MC224221

Ttc21a (untagged) - Mouse tetratricopeptide repeat domain 21A (Ttc21a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ttc21a
• Synonyms: 4921538N17Rik; AA387572; Thm2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224221 representing NM_028735
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAGCGATGACTCCTCTCTCATGGCTTCGATCATTTACTACAGCCAGGAAAAGTACTTCCACCATG
  TGCTGCAGGCTGCGAATGTGGGCCTAGAGAGGTACAGCAGCGACCCAGCCCTGCAGTTCTTCAGAGCGTA
  TGGAATCCTTGGGGAAGACCACATCCACGATGCCATCAGCGAGCTGGAAGGCATCCAAAGCCACCCAGAC
  ACGTCCCTGTGCGCGGTCATCGCCCTGCTTTCTGCTCACAAATCCTGCGACACCATCGACATAGAAGCAG
  TTCAGGAACTGGAGAGCAGCTTGAAGGAAATTCGCAGGTCAGCCAGCAGCACTGCTCTGTACTACGCCAG
  CCTCTTCCTCTGGTTCATGGGTCACCACGACAAGGCCAGGGAGTATGTTGACCACATGTTGAAGGTCTCC
  AGCGGCTCCAAAGAGGGCTATGTGCTCAAAGGCTGGGTGGACCTTACCTCCAGTAAACCCCACGTTGTGA
  AGAAATCCATCAAGTACCTGGAACAAGGAACTCAAGACACCAAAGATGTGCTAGGGCTCATGGGCAAGGC
  AACGTACTTCATGACACAACAGAACTTCTCTGGGGCCCTGGAGGTGGCGAACCAGGTCACCTTGGCCTGC
  AGCAACTTCCTGCCCGCTTTAGTCCTGAAGATGAAGCTGTTCCTGGCTCGGCAGGATTGGGATCAGACGA
  TAGAAACCGGACAAAGAATCCTTGAAAAAGACGAAAACAATATTGATGCCTGGCAAATTCTAGCTGTGCA
  CGAGCTGGTGAAAGAAGGGAACACGGACAGAGCTGCCGACCGGGTCAGAAACCTCATCAAGGCGCTAGAG
  ACTGGAGAGCCCCACAATCCAAACCTACATCTTAAGAAGATCCTTGTAATCAGCAGGCTGTGTGGGAGGC
  ACCAGGTGGTCCATCGGCTTGTGAGCGGCTTCCTGGAGCGCATCTTTATGGCCACACCTTCCTGTGCCCT
  TGTGGCCACAGAGCTGGGCTACCTCTTCATCCTACACCAACAAGTGAAGGAAGCCTGTCTGTGGTACAAG
  GAGGCCATGAAGCTGGAGGAGAACAGGCTGGCTGCCTTGGCAGGGAGCATCTGGTGCCAGATCCTACAGG
  GCCAGCTGGAGGAGGCTGCTCACCAGCTGGAGTTCCTCAAGGAGGTGCAGCAGTCCCTTGGGAAGTCTGA
  GTTGCTGGTTTTCCTGCAAGCCCTCGTAGCAGCTAAGAAGCAAAGGCTGGAGCAGGAGGCCACGGCGCTC
  CTGAAGGAGGCGGTGGAAGTGCACTTCTCCAGCATGCAGGGTCTGGCCCTGAGCCCCGAGTACTTTGAAA
  AGCTGGACCCCCTCTTTCTGGTCTGCATCGCCAAGGAGTATCTGCATTTCTGCCCCAAGCAGCCCCGGTC
  ACCCGGCCAGCTCGTATCTCCACTTCTTAAACAAGTCGCCATGATCTTGAGTCCTGTGGTGAAGGTGGCC
  CCGGCCATGATGGAGCCACTGTATGTAACGGCTCAGGTCAAGTTCCTCTCAGGAGAGCTGGAAAATGCGC
  AGAGCACCCTGCAGCGCTGCCTGGAGCTGGACCCCACATTTGTGGATGCTCACCTCCTCATGTCCCAGAT
  CTACCTGGCCCAGGGCAACTTCGCCATGTGCTCCCATTGCCTAGAGCTGGGTGTCAGCCACAACTTCCAG
  GTCCGAGACCATCCCCTCTACCACTTCATCAAAGCCAGGGCCCTCAATAAGACGGGCGACTATGCCGAGG
  CCATAAAGACCTTAAAAATGATCATAAAGGTTCCCACCTCGAAGGCAGAAGAGGGCAGGAAGTCCCAAAG
  CCCCTCCGTTCGGCCCAGCGAGCGGGCATCCATCCTGCTAGAGCTAGTAGAGGCTCTCCGACTGAATGGG
  GAACTGCACGAGGCCACCAAGATCATGCAGGACGCCATCAATGAGTTCAGCGGCACGCCAGAGGAGATGC
  GCATCACGGTCGCCAACGTGGACTTGGCTCTGAGCAAGGGCAACGTGGACCTGGCGCTGAACATGTTAAG
  AGGTATCACACCCAAGCAACCCTGCTACACAGAAGCCAAGGAGAAGATGGCCAGTATCTACCTGCACACT
  CGCAAGGACGTGCGCCTCTACATTGGCTGCTACGTGGAGCTTTGTGAACATCTGCCCGGCCCCCACAGCA
  GCCTGCTACTGGGTGACGCTTTCATGAACATTCAGGAGCCTGAGAAGGCCCTGGAGGTGTATGACGAGGC
  CTATAGAAAGAACCCTCACGATGCCTCCTTGGTCAGCAGGATCGGACAGGCCTACGTGAAGACCCACCAG
  TACGCCAAGGCCATTAATTACTACGAGGCTGCCCAGAAGATCAGCGGGCAGGACTTCCTGTGCTGCGAGC
  TGGCCGAGCTCCTCCTCAAGCTGAAGAAATACCACAAGGCAGAGAAGGTGCTGAAGCAGGCCTTGGAGCG
  AGACTCAGGGGTCAAGGACATCCCATCCATGATAAATGAGGTCAAGTGCTTGCTTTTGTTGGCAAAGGTT
  TACAAGAGCCACAAGAAGGAAGAGGTGATGGAAACCCTGAATCTGGCCTTGGACCTCCAGTCCCGGATAC
  TGAAGCGGGTTCCTTTGGAGCAATCAGAAATGATCCCCTTCCAGAATCAGCTGGCAGCCTCCATCTGCAT
  CCAGATTGGGGAACATCACCTGGCGGAGAAAGATTACGACAGCGCCCTGAAATCCTATAAGGACGCCCTC
  GCCTACTCGCCCACAGACAGTAAGGTGGTGCTGGAGCTGGCCCATCTCTACCTGCTTCTGGGTCAGCTGG
  ACCTGTGTGAACAGCGCTGTGCCCCCCTCTTAGAGATGGAGCAGACCCACGAGAGAGCCGCTGTGATGCT
  GGCCGACCTGATGTTTAGAAGACAAAACTATGAAACTGCCATCAATCTCTACCACCAAGTGCTGGAGAAA
  GCTCCAGACAATTTTTTGGTATTGAATAAACTGGTCGATCTGCTACGACGGAGCGGCAAGCTTGAAGAGG
  CCCCTGCTTTCTTTGAACTGGCCAAGAAGGTGTCTAGCCGGGTGCCTTTGGAGCCAGGGTTTAACTACTG
  CCAAGGCATTTACTTCTGGCACATTGGACAGCCCAATGAAGCCTTGAGGTTTCTGAACAAAGCCCGAAAG
  GACAGCACTTGGGGCCAGCTTGCCACCTGTTACATGGTACAGATCTGTCTGAACCCTGACAATGAGATTG
  TCGGTGGAGAGGCTTTTGAGAGCCTGGTGGGTGACAGCAACTCTGCCAGTAGAAAGGAGTCTCAGCAGCA
  CGGGGTGCGCACTGCAGAGAAGCTACTTCGGGAATTTTACCCACACTCGGAATCCGGGCAGACCCAGCTG
  CGGCTGCTGCAGAATCTCTGCCTGCTGGCCACCAGGGAGAAGGCGAATGTAGAGGTGGCTCTGGGAGCCT
  TTATCGAGATGGCCCAGGCTGAGAAGGACAGCATCCCTGCACTGCTGGCCATGGCGCAGGCCTACATACT
  CCTGAAGCAAGTCCCCAAGGCCCGAACGCAGCTGAAGCGCCTGGCCAAAGTCCCATGGACGGTCGACGAG
  GCCGAAGACCTGGAAAAGAGCTGGCTTCTGTTGGCAGATATCTATTGCCAAGGCGGCAAGTTCGACCTGG
  CCTTGGAGCTACTGCGCCGATGCCTGCAGTATAACAAGTCTTGCTGCCGGGCCTATGAGTACATGGGCTT
  CATCATGGAGAAAGAGCAGTCATACAAGGATGCAGCAACCAACTACGAGCTGGCCTGGAAGTACAGTCAT
  CAGGCCAACCCCGCCATTGGTTTCAAACTGGCTTTCAACTACCTGAAAGACAAGAAGTTCGTAGATGCCA
  TTGAAGTCTGTCACAGCGTCCTGACGGAGCACCCCAAGTACCCCAAGATCAGGGAGGAGATTTTGGAGAA
  AGCCCAAGGGTCCTTGAGGCCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_028735
• Insert Size: 3945 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028735.3, NP_083011.2
• RefSeq Size: 4182 bp
• RefSeq ORF: 3945 bp
• Locus ID: 74052
• UniProt ID: Q8C0S4
• Cytogenetics: 9 F4
• Gene Summary: Intraflagellar transport (IFT)-associated protein required for spermatogenesis (PubMed:30929735). Required for sperm flagellar formation and intraflagellar transport (PubMed:30929735).[UniProtKB/Swiss-Prot Function]