Kif16b (NM_001081133) Mouse Untagged Clone

CAT#: MC224215

Kif16b (untagged) - Mouse kinesin family member 16B (Kif16b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kif16b
• Synonyms: 8430434E15Rik; AA623607; AI789011; C80253; C80902; mKIAA1590
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224215 representing NM_001081133
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATCGGTCAAGGTGGCCGTGAGAGTCCGGCCCATGAATCGCAGGGAAAAGGACCTGGAGGCCAAGT
  TCATCATTCAAATGGAGAAAAGCAAAACGACAATCACAAACTTAAAGATACCAGAAGGAGGAACTGGGGA
  TTCAGGCAGAGAACGGACCAAGACCTTCACCTATGACTTCTCTTTTTATTCTGCTGACACCAAGAGTCCA
  GATTATGTCTCACAAGAAATGGTTTTCAAAACTCTGGGTACAGATGTTGTGAAATCTGCATTTGAAGGTT
  ACAATGCTTGTGTCTTTGCATATGGGCAAACTGGATCTGGAAAGTCCTACACCATGATGGGAAATTCTGG
  CGATTCTGGCTTAATACCTCGGATCTGTGAAGCTCTCTTCAGTCGGATCAATGAAACCACTAGATGGGAT
  GAAGCCTCTTTCAGGACAGAAGTCAGCTACTTAGAAATTTATAATGAACGTGTGAGAGACCTACTTAGGC
  GGAAGTCATCTAAGACCTTCAATTTAAGAGTCCGTGAGCATCCCAAAGAAGGACCGTACGTTGAGGATTT
  ATCCAAGCATTTAGTCCAGAACTACAGTGATGTGGAGGAACTCATGGACGCAGGAAATATCAATCGCACC
  ACCGCAGCGACGGGGATGAATGATGTCAGTAGCAGGTCCCACGCCATCTTCACCATCAAGTTCACTCAGG
  CAAAATTCGACGCTGAAATGCCATGTGAAACTGTGAGCAAGATCCACCTCGTCGATCTTGCTGGAAGTGA
  GCGGGCAGATGCCACCGGTGCCACTGGGGTCAGACTCAAGGAAGGGGGCAATATAAACAAGTCCCTTGTG
  ACTCTGGGAAATGTCATTTCTGCCTTAGCTGATTTATCTCAGGATGCAGCCAACCCTCTTGTGAAGAAGA
  AGCAAGTTTTTGTGCCTTACAGGGATTCAGTTTTGACTTGGCTGTTGAAAGACAGCCTTGGAGGAAACTC
  TAAAACCATCATGATTGCCACCATTTCACCTGCTGATGTCAATTATGGAGAAACCCTAAGTACCCTTCGC
  TATGCAAATAGAGCCAAAAACATCATCAACAAGCCTACCATTAATGAGGATGCCAACGTCAAACTCATCC
  GTGAGCTGAGAGCTGAAATAGCCAGGCTGAAAACCCTGCTCGCTCAAGGGAACCAGATTGCCCTCTTGGA
  CTCCCCTACAGCTTTAAGTATGGAGGAAAAACTTCACCAGAATGAAGCAAGAGTCCAAGAATTGACCAAG
  GAATGGACAAACAAGTGGAATGAAACCCAGAATATTTTGAAAGAGCAAACTCTAGCCCTCAGGAAAGAGG
  GCATTGGTGTGGTGCTGGACTCGGAGCTGCCGCACCTAATTGGCATTGATGATGATCTTCTGAGCACTGG
  AATCATCTTGTATCACTTGAAGGAGGGTCAGACTTACGTCGGCAGAGAAGATGCTTCAACAGAGCAAGAT
  ATTGTCCTTCATGGCCTTGACTTGGAGAGTGAGCACTGTGTCTTTGAGAACGCCGGGGGAACAGTGACTC
  TGATACCCCTGAGAGGGTCCCAATGCTCTGTGAATGGTGTTCAGATTGTGGATGCCACGCAGCTAAATCA
  AGGTGCTGTGATACTCTTGGGAAGAACCAACATGTTCCGCTTTAACCATCCGAAGGAAGCCGCCAAGCTT
  AGGGAGAAGAGGAAGAGTGGCCTCCTGTCCTCCTTCAGCTTGTCTATGACCGACCTCTCAAAGTCCTGTG
  AGAACCTGTCTGCAGTCATGTTGTATAACCCTGGACTTGAGTTTGAGAGACAACAGCGTGAAGAACTTGA
  AAAACTCGAAAGTAAAAGGAAACTCATTGAGGAGATGGAGGAAAAGCAGAAGTCGGACAAGGCAGAACTA
  GAGCGAATGCAGCAGGAGGTAGAGACCAGGCGCAAGGAAACAGAGATTGTGCAGCGGCAAATCCGCAAGC
  AGGAGGAAAGCCTCAAACGCCGCAGCTTCCACATTGAAAACAAGCTCAAGGACCTGCTGGCTGAGAAGGA
  AAGGTTTGAGGAAGAGAGGCTTCGGGAGCAGCAGGGACTCGAGCAGCAGAGGAGGCAGGAGGAAGAGAGC
  TTATTCCGCATCAGAGAAGAGCTTCGCAAACTCCAAGAGCTCAACAGCCACGAACAAGCTGAGAAGGTCC
  AGATATTCCAGGAGCTGGACCGATTGCACCAAGAGCAAAATGCCCAGAGTGCCAAGCTGCGGTTGGAGAA
  AAGGAGGCTGGAGGAGGAGGAGAAGGAGCAGGTGCAGCGGGTGGCACACCTGGAGGAGCAGCTTCGGAAG
  AGGCAGGACACTGCTCCTCTGCTGTGTCCTGGAGAGGCCCAGCGGGCCCAAGAGGAAAAGAGGGAACTGG
  AAAGCATCCGGGAAGCCCTTTTGCAGGCCAAAGAGATGAGAGCTGGAGGGGACCATACCTGTAGGGATGA
  ACTGGAAAGGGCTCAGCAGTACTTCTTAGAGTTCAAGAGAAGGCAGCTTGTCAAGCTAGCAAGCCTGGAG
  AAGGACCTGGTCCAACAGAAAGACCTCCTAAGCAAAGAAGTCCAAGAAGAGAAAGTAGCTCTGGAGCATG
  TAAAATGTGACGCAGGGGGTGACCCGAGCTTCTTGGCAACAGATGATGGCAACATTCTCGGTGGGCCTCC
  AGATCTAGACAAAATAAAGACTGCAGAGACCAGGCTACAGAGCAGAGAGCACCAGCTACAGGACCTTCTG
  CAAAATCATTTGCCAGCTCTGCTGGAAGAAAAGCAGAGAGTGCTTGATGCCCTTGACAGTGGTGTCCTAG
  GCCTAGACACCACTCTCTGCCAAGTAGAGAAAGAAGTGGGAGAGAAAGAAGAGCAGATCGCACAGTACCA
  GGCTAATGCTAGCCAGCTGCAACAGCTCCGGGCCACCTTCGAGTTCACAGCTAACGTGGCCAGACAGGAA
  GAGAAGGTGAGAAGAAAGGAAAAGGAAATACTGGAGTCCCAGGAGAAACAGCAAAGAGAGGCATTGGAGC
  AGGCTGTGGCTAAGCTAGAGCAGAGGCGCTCTGCCCTGCAGCGGTGCTCCACCCTGGACCTGGAGATCCA
  GGAGCAGAGGCAGAAACTTGGCTCTCTCCACACCAGTGAATGGTCAGGGTGGCAGGCCAGTCTGGAGACT
  GATGGAGAAGCACTTGAGATGGACCCTGCAAGGTTAGAACATGAAATCCATCAGCTGAAGCAGAAGATCT
  GTGAAGTAGATGGTGTTCAGAGACCTCATCATGGGATTTTAGAGGGGCAGGCCGTTCTCTCCAGCTTGCC
  ACCCAGTGGGGGAAACTCTCACCTGGCTCCACTGATGGATGCCAGGATCAGTGCTTACATTGAAGAAGAA
  GTCCAGAGACGCCTTCATGATTTGCACAGGGCAATTGGTGATGCCAACCATACACCTGCTGATGTGATGA
  AGAGTAATGAGGAACTTCACAATGGCACCACTCAACGTAAGCTAAAGTATGAGCGGATGTATTCTCGCTC
  TTTGGGAACAAATCGAGACGACCTGAAGGACCCCATTAAGATTAGTATTCCTCGCTATGTCCTGTGTGGC
  CAAGGGAAGGACGAGCACTTCGAGTTTGAAGTGAAGATTTCTGTCCTAGATGAGACATGGACTGTATTCA
  GGCGCTACAGTCGTTTTCGAGAAATGCATAAAACATTGAAGCTAAAGTATGCAGAGCTCGCTGCTCTTGA
  ATTCCCCCCAAAGAAGCTGTTTGGAAACAAAGACGAGCGGGTAGTTGCTGAGCGGCGGACACACTTAGAG
  AAATACCTCAGGGAGTTTTTCAGTGTGATGCTCCAGTCCGAGACATCTCCCCTTCACATCAACAAAGTGG
  GACTGACCCTGTCCAAGCACACCATTTGTGAATTCTCACCATTCTTCAAGAAAGGAGTTTTTGACTACAG
  CAGCCATGGGACAGGGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081133
• Insert Size: 3939 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081133.2, NP_001074602.1
• RefSeq Size: 5446 bp
• RefSeq ORF: 3939 bp
• Locus ID: 16558
• UniProt ID: B1AVY7
• Cytogenetics: 2 G1
• Gene Summary: Plus end-directed microtubule-dependent motor protein involved in endosome transport and receptor recycling and degradation. Regulates the plus end motility of early endosomes and the balance between recycling and degradation of receptors such as EGF receptor (EGFR) and FGF receptor (FGFR). Regulates the Golgi to endosome transport of FGFR-containing vesicles during early development, a key process for developing basement membrane and epiblast and primitive endoderm lineages during early postimplantation development.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data.