Mapk8ip3 (NM_001163451) Mouse Untagged Clone

CAT#: MC224198

Mapk8ip3 (untagged) - Mouse mitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3), transcript variant 6, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mapk8ip3
• Synonyms: BB120594; D17Wsu15e; JIP-3; Jip3; JSAP1; JSAP1a; JSAP1b; JSAP1c; JSAP1d; mKIAA1066; Syd2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224198 representing NM_001163451
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGAGATCCAGATGGACGAGGGAGGAGGAGTGGTGGTGTACCAAGACGACTACTGCTCGGGCTCGG
  TCATGTCGGAGCGTGTGTCGGGCCTGGCGGGCTCCATCTACCGCGAGTTCGAGCGCCTCATTCACTGCTA
  TGACGAGGAGGTGGTCAAGGAGCTCATGCCGCTGGTGGTGAACGTGCTGGAGAACCTTGACTCGGTGCTG
  AGCGAGAACCAGGAGCACGAGGTGGAGCTGGAGCTCCTACGCGAGGACAACGAGCAGCTGCTCACGCAAT
  ACGAGCGCGAGAAGGCGCTGCGCAAACAGGCCGAGGAGAAATTCATCGAATTTGAAGATGCCTTGGAACA
  AGAGAAGAAAGAACTCCAGATCCAGGTAGAACATTATGAGTTTCAGACACGCCAGCTGGAGCTAAAGGCC
  AAAAACTATGCAGATCAGATTTCCCGACTGGAGGAACGAGAATCGGAGATGAAGAAGGAATACAATGCCC
  TGCACCAGCGGCACACAGAGATGATCCAGACCTATGTGGAACACATTGAAAGATCCAAGATGCAGCAAGT
  TGGGGGTAGCGGCCAAACAGAAAGCAGCCTGCCCGGGCGGAGGAAGGAGCGTCCCACCTCTCTGAATGTC
  TTCCCCCTGGCTGATGGCATGTGTCCAAATGATGAGATGTCTGAGTCAGGCCAGTCCTCAGCAGCTGCAA
  CACCCAGTACCACAGGTACCAAGTCCAACACACCCACGTCCTCCGTGCCCTCAGCAGCAGTCACGCCACT
  CAACGAGAGCCTACAGCCCCTGGGGGACTATGTCAGTGTCACAAAGAACAACAAGCAGGCCCGAGAGAAG
  CGCAATAGCCGTAACATGGAGGTCCAGGTCACCCAAGAGATGCGGAACGTCAGTATCGGCATGGGCAGCA
  GTGACGAGTGGTCCGATGTTCAGGACATTATCGACTCCACCCCAGAGCTGGATGTGTGTCCTGAAACCCG
  TCTGGAGCGCACAGGAAGCAGCCCAACCCAGGGAATTGTAAACAAAGCTTTTGGAATCAACACTGACTCC
  TTGTATCACGAACTCTCCACGGCGGGATCTGAGGTCATCGGGGATGTGGACGAGGGAGCTGATCTCCTAG
  GGGAGTTTTCAGTGCGCGATGATTTTTTTGGAATGGGCAAAGAAGTGGGGAACCTGCTGCTGGAGAACTC
  ACAGCTTCTAGAGACAAAAAATGCTTTAAATGTAGTGAAGAATGACCTCATTGCTAAGGTTGACCAACTG
  TCAGGAGAACAGGAGGTCCTGAAGGGTGAGCTGGAAGCAGCCAAGCAAGCCAAAGTCAAGCTGGAGAACC
  GAATCAAAGAGCTTGAAGAAGAACTGAAGAGAGTCAAGTCAGAGGCAGTAACTGCCCGCCGTGAGCCCAG
  AGAAGAGGTGGAGGATGTAAGCAGCTATCTCTGTACAGAATTGGACAAAATCCCCATGGCCCAGCGCCGA
  CGCTTCACACGGGTGGAGATGGCCCGAGTGCTCATGGAACGCAACCAGTACAAGGAACGCCTCATGGAGC
  TGCAGGAGGCTGTGAGGTGGACTGAAATGATCAGAGCATCAAGGGAACACCCATCTGTCCAGGAGAAGAA
  GAAGTCCACCATCTGGCAGTTCTTTAGTCGCCTCTTCAGCTCCTCATCTAGCCCCCCTCCGGCCAAACGA
  TCCTACCCATCTGTGAACATTCACTACAAGTCACCCACTGCAGCTGGCTTTAGCCAGCGTCGCAGCCATG
  CTTTGTGCCAGATCTCAGCCGGCAGCAGGCCCCTGGAGTTCTTCCCTGATGATGACTGCACCTCTTCTGC
  CCGGCGGGAGCAGAAGCGGGAGCAGTACCGCCAGGTTCGTGAACACGTGCGCAATGATGACGGGAGGCTG
  CAGGCCTGTGGGTGGAGCCTGCCTGCCAAGTACAAGCAGCTGAGCCCCAATGGAGGCCAGGAAGACACCC
  GGATGAAAAATGTGCCTGTCCCTGTGTACTGCCGCCCTCTGGTGGAGAAGGACCCTTCGACAAAGCTGTG
  GTGTGCTGCTGGTGTCAACCTGAGTGGGTGGAAGCCACATGAAGAGGACTCTAGCAATGGACCCAAGCCT
  GTACCAGGTCGAGACCCTCTGACCTGTGACCGGGAAGGAGAAGGCGAACCCAAGAGCACACACCCATCAC
  CTGAGAAGAAGAAGGCAAAGGAAACCCCTGAGGCAGATGCTACCTCCAGTCGGGTATGGATCCTCACCAG
  CACCCTGACAACCAGCAAGGTGGTGATCATTGATGCCAACCAGCCAGGCACAATTGTGGATCAGTTCACA
  GTCTGCAATGCCCACGTCCTGTGTATCTCCAGCATTCCTGCGGCCAGTGACAGTGACTATCCCCCTGGGG
  AGATGTTCCTAGACAGTGATGTGAACCCTGAAGATTCAGGTGCTGATGGTGTGCTGGCTGGCATCACCCT
  GGTGGGGTGTGCTACCCGCTGCAATGTTCCACGTAGCAACTGTTCCTCACGAGGAGACACCCCAGTACTG
  GACAAGGGGCAGGGGGATGTGGCGACCACTGCCAATGGGAAGGTCAACCCGTCCCAATCCACAGAAGAAG
  CCACAGAAGCCACGGAGGTGCCAGACCCTGGTCCCAGCGAGTCAGAAGCAACGACAGTCCGGCCCGGGCC
  TCTCACAGAGCATGTCTTTACTGACCCAGCACCCACCCCATCCTCCAGCACCCAGCCTGCCAGTGAGAAT
  GGGTCAGAGTCCAATGGCACCATTGTACAGCCTCAGGTGGAGCCCAGTGGGGAACTCTCAACAACAACCA
  GTAGCGCTGCACCCACTATGTGGCTAGGAGCCCAGAATGGCTGGCTCTATGTGCATTCAGCGGTAGCCAA
  CTGGAAGAAGTGTCTGCACTCCATCAAGCTAAAAGACTCTGTGCTGAGCCTGGTGCATGTCAAAGGCCGA
  GTGCTGGTAGCTCTTGCAGATGGGACCCTGGCTATCTTCCATCGTGGAGAGGATGGCCAGTGGGACCTGA
  GCAACTACCACCTAATGGACCTGGGCCACCCACACCACTCCATCCGCTGCATGGCTGTTGTGAATGACCG
  AGTTTGGTGTGGCTACAAGAACAAGGTGCATGTTATCCAGCCCAAGACAATGCAGATTGAGAAATCATTT
  GATGCCCACCCAAGGCGGGAAAGCCAGGTACGTCAGCTGGCCTGGATCGGTGATGGAGTGTGGGTCTCTA
  TTCGCTTGGATTCTACCCTTCGGCTCTACCATGCTCACACCCACCAGCACCTGCAGGATGTGGACATTGA
  GCCCTATGTTAGCAAGATGCTAGGAACCGGCAAGCTGGGCTTCTCCTTCGTGCGCATCACAGCCTTACTC
  ATTGCAGGCAACCGTCTGTGGGTGGGCACTGGCAATGGGGTTGTCATCTCCATCCCCTTGACTGAGACTG
  TGGTCCTGCATCGAGGCCAGCTCCTAGGGCTCCGAGCCAACAAGACATCCCCAACATCTGGGGAGGGGAC
  CCGCCCAGGGGGCATCATCCATGTGTATGGGGACGACAGCAGTGACAAGGCCGCCAGTAGTTTCATCCCC
  TACTGCTCCATGGCACAGGCTCAGCTTTGCTTCCATGGGCACCGTGATGCTGTCAAATTCTTTGTCTCTG
  TGCCAGGAAATGTGCTGGCCACTCTCAATGGCAGTGTGCTAGACAGCCCATCAGAGGGCCCTGGGCCTGC
  TGCACCCGCTGCAGATGCTGAGGGCCAGAAGTTGAAGAATGCACTGGTGCTGAGTGGTGGTGAAGGTTAC
  ATTGACTTCCGTATCGGAGACGGAGAGGATGATGAAACTGAGGAATGTGCCGGGGACGTGAACCAGACAA
  AGCCCTCGTTGTCCAAGGCTGAGCGCAGCCACATCATCGTGTGGCAGGTGTCCTACACCCCTGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001163451
• Insert Size: 3918 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163451.1, NP_001156923.1
• RefSeq Size: 5483 bp
• RefSeq ORF: 3918 bp
• Locus ID: 30957
• UniProt ID: Q9ESN9
• Cytogenetics: 17 12.53 cM
• Gene Summary: The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins (PubMed:10523642, PubMed:10629060). Promotes neuronal axon elongation in a kinesin- and JNK-dependent manner (PubMed:23576431, PubMed:25944905, PubMed:28259553). Activates cofilin at axon tips via local activation of JNK, thereby regulating filopodial dynamics and enhancing axon elongation (PubMed:23576431, PubMed:25944905, PubMed:28259553). Its binding to kinesin heavy chains (KHC), promotes kinesin-1 motility along microtubules and is essential for axon elongation and regeneration (PubMed:23576431, PubMed:25944905, PubMed:28259553). Regulates cortical neuronal migration by mediating NTRK2/TRKB anterograde axonal transport during brain development (PubMed:23576431, PubMed:25944905, PubMed:28259553). Acts as an adapter that bridges the interaction between NTRK2/TRKB and KLC1 and drives NTRK2/TRKB axonal but not dendritic anterograde transport, which is essential for subsequent BDNF-triggered signaling and filopodia formation (PubMed:23576431, PubMed:25944905, PubMed:28259553).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (6) uses two alternate in-frame splice sites in the 5' coding region compared to variant 1. This results in a shorter protein (isoform f) compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.