Tbc1d32 (NM_001033385) Mouse Untagged Clone

CAT#: MC224194

Tbc1d32 (untagged) - Mouse RIKEN cDNA D630037F22 gene (D630037F22Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tbc1d32
• Synonyms: b2b2284Clo; Bromi; C6orf170; D630037F22Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224194 representing NM_001033385
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCATTTCTCCAGCGAGGATGAGGTGATGCTGCAGGCGATGCTCCGGCAGCTGTTCCAGAGCGTGA
  AGGAGAAAATCACCGGAGCTCCCTCCTTGGAGTGCGCAGAGGAGATCCTCTTGCGGCTGGAGGAGACTGA
  CGAAAATTTTCACAACTATGAATTTGTGAAATACCTCAGACAGCACATATGTAACATGTTGGGGTCTATG
  ATTGAAGAAGAAATGGAAAAATGCACATCTGATCAGAATCAGGGGGAGGATTCTGGCTATGACACGGTTG
  TTCAGCATGTTACCAAAAGAACGCAGGAATCTAAAGAGTATAAGGAAATGATGCATTCCCTGAAGAACAT
  TATGATGGTGGTGGTCGAGGCGATGATCAACAAGTTTGAGGAAGACGAGACACGGAGTGAGGACAGGCAG
  AGGAAAATGCAGAGTGGCAGCTGCTGCACAGACAACTGCTCCGACAGTGACTCCTCCTTCAATCAGAGTT
  ACAAATTCTGTCAAGGGAAACTACGGTTGATTTTAGACCAGTTGGATCCTGGCCAACCTAAAGAGGTGAG
  ATATGAAGCTTTACAAACATTGTGCTCAGCTCCCCCATCCGACGTCCTGAGCTGTGAGAACTGGACCACT
  CTCTGTGAAAAGCTGACCACATCTCTGTCTGATCCAGATCCTATGTTCACTGACCGGATTTTAAAGTTCT
  ACGCACAGACTTTTACCCTCTCACCGTTACATATGACCAAGGAGATTTATACAAGCTTAGCTAAATATCT
  GGAGGTATATTTTCTTTCTAGAGAAAATCACCTTCCTACTCTTTCAACTGGAGTAGACATAACCAGTCCC
  AATGTGACCCGCTTACTGAAAAAGGTTCGCCTTCTGAATGAGTACCAGAAAGAGGCTCCATCTTTTTGGA
  TTCGTCACCCAGAGAAGTATATGGAGGAAATTGTGGAGAGTACTCTGTCCTTGCTCTCAGTCAAACACGA
  GCAAAGCCACCTGGTGCCACAGAAGATTTTGGATCCCATCTATTTTTTCGCATTAGTTGATACTAAGGCT
  GTGTGGTTCAAAAAGTGGATGCATGCATATTACAGCAGAACTGCAGTACTGAGACTTCTTGAAAAGAAAT
  ATAAATGTCTGATAACTACAGCAGTTCAGCAATGTGTTCAGTACTTGGAGTTATGTGAAGCTATGAAAGC
  TGATGAAATTCTGAGACATCCAAAACATTGTGGAACCAAGCAAAAGAGTTTCTACTATTCAGGACAAGAA
  TTGCAATATATTTATTTCATTCACTCACTGTGCCTTTTAGGAAGATTGTTGATCTATACACAGGGCAGGA
  AATTATTCCCCATAAAGCTGAAGAATAGAAAAGATTCAGTATCCCTGACAAATCTGCTGGTTTTGTTTAC
  TCAACTTATCTATTATTCACCAAGTTGTCCAAAGATGACCTCCATCATGTGTTCAGAGAACTATTCTCCT
  GCAAGTATGGTGACTGACGTTCTGCGGATGCTCTGCGACCAGAAAGAATGTGCAGTCGAGTGCTTATACA
  ACAGCACAGTGACAGAGGCGCTTCTCCTGCCCATTCATAACCTGACGAAAGGAACTGCGGCTGCTCCAGA
  TTGCTCTGAGACAGCTCTAATTCATATAGCTGATATTTTGGCAAGAATCGCTTCTGTCGAGGAAGGACTC
  ATCTTACTTCTGTATGGAGAAAATATGAACTCTTCTGAAGAAGAAAGTCTTACAGGTGCTCATATAATAG
  CCAAGTTTTCAAAGAAACTTCTTGAAGAAGATATTTCTATTTTTTCTGGATCGGAAATGTTGCCTGTAGT
  TAAAGGAGCTTTTATTTCTGTGTGTCGTCAGATCTACGGTACCTGCGAAGGCTTACAGGTGTTGCTTCCT
  TATGGTTTACATGAATCCATAGCAAAGGCATGGAAGAAGACAAGCTTGCTATCAGAAAGGATCCCCACTC
  CAGTAGAGGGCTCTGATTCTGTTTCCTCAGTGAGCCAGGTGTCTCCGAACAGTGTGGCTTGGGAAGACAA
  TTTGTTGGATGATTTACTGAATTTTGCAGCCACCCCCAAAGGACTGCTGCTTCTTCAGAGAACAGGCGCC
  ATCAATGAATGTGTGACGTTTATGCTCAGCCAGTATGCAAAAAAGCCACAGGTCAACAGGCAGAAGAAGT
  TTGGCTATGAAGTCTTGGTTGTACAGGTGGCATCGACAGCAGCAGGTGCAGTGGCTCTACAGAATTCAGG
  CTTTATTAGTGCACTTATAACTGAGTTATGGTCCAACCTTGAATGTGGAAGAGATGATGTAAGGCTAACT
  CATCCCAGAGCTACCCCAGTGGACCCAATTGACCGAAGCTGTCAGAAGTCCTTCCTTGCACTGGTGAACC
  TGCTGTCCTACCCTGCTGTGTATGAGCTAACAGCCAATCAGGAGCTTCCTAATAAGGCAGAGTATTCTCT
  TCGTGAAGTTCCCACATGCATCATCGATATAATGGATAGGCTTATTGTTTTGAACTCTGAAGCCAAGATC
  CGGTCTTTACTGAATTATGAACAGTCGCACACCTTTGGTCTAAGGTTACTAAGTGTGGTGTGCTGTGATC
  TGGATGCGCTTCTCTTACTGGAGGCTCAGTATCAAGTATCTAACATGTTATTACATGCTCAAGAAGAAAA
  TACCTTTGAGATCTCTGAGAACCACAGGAACTTTATAATTGATGGATTATCAGTAGAGAGAAATCATGTT
  CTCGTAAGGATTAATCTTATTGGTGGTCCTTCAGAGCGGATATTGCCTCCAAGGATGCTTGAGAAGGGGG
  ATGACCCATATCCTTGGCCAATGTTTTCTTCATATCCATTGCCACACTGCTATCAGTCAGAAGGTCCACG
  AAGTGCTGATCTAAAACAAGACAATGATATTGGAAACCTGTTATCATGCTTCAAAATGTCCGATAAACAA
  ACAGAGTGGATAGAGAACTGCCGAAGACAGTTTTGTAAAACAATGAAGTCCAAGCCTGATGCAGTCCATG
  GAAGTGCCTTAGGAGAACTACTGGAAAAATTTGTGCTGCTTCTCACCGAAAACCCATCTGAATGTTACTT
  CCCTTCAGTGGAATACACAGCTACTGATGCAAATGTGAAGAATGAAAGTCTCTCATCTGTGCAGCAGCTT
  GGCATGAAAATGACTGTCAGGTATGGGAGGTTTCTCAATTTGTTAAAAGATGGTGCAGAAAATGAGCTTG
  CTTTGGTCTTAAAGCATTGTGAGAAATTCCTGAAACAACAGCAGTCACCGGTCACTTCTTCTCTCCTGTG
  CCTGCAAGGGAACTACGCAGGCCATGACTGGTTTGTGTCTTCTCTGTTCATGATAATGCTGGGAGACAAA
  GGGAAGACCTTCCACTTTCTCCAGCATTTCTCCAGGCTGCTGACCTCTGCCTTCCTTTGGGTGCCACGGC
  TGCATAATTCTAGGTACCTTCCTGTCGACACCCTAGGGACCGGCATCCATCCCATCTACTTTTGCAGTGC
  TCACTACATTGAAATGCTGCTGAAGGCTGAGGTGCCGCTTGTGTTTTCGGCTTTCCACATGTCTGGTTTT
  GCTCCATCCCAGATTTGCCTGCAGTGGATAACTCAGTGTTTTTGGAATTACTTAGATTGGATAGAAATAT
  GCCACTATATTGCTACTTGTGTTGTCCTTGGTCCTGATTATCAAGTGTATGTCTGCATAGCCGTTCTCAA
  ACATCTGCAGCGAGATATTCTCCAGCATACCCAGACTCAAGATCTGCAAGTCTTTCTCAAAGAAGAAGCA
  CTGCATGGGTTTCGAGTGAGCAACTATTTTGAATACATGGAGAATTTGGAGCAAAACTACCGACCAGTGC
  TGCTGCGAGACATGCGCAGCATCAGAGTGCAGAACACATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001033385
• Insert Size: 3891 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033385.3, NP_001028557.2
• RefSeq Size: 7269 bp
• RefSeq ORF: 3891 bp
• Locus ID: 544696
• UniProt ID: Q3URV1
• Cytogenetics: 10 B3-B4
• Gene Summary: Required for high-level Shh responses in the developing neural tube. Together with CDK20, controls the structure of the primary cilium by coordinating assembly of the ciliary membrane and axoneme, allowing GLI2 to be properly activated in response to Shh signaling.[UniProtKB/Swiss-Prot Function]