Ptprj (NM_001135657) Mouse Untagged Clone

CAT#: MC224183

Ptprj (untagged) - Mouse protein tyrosine phosphatase, receptor type, J (Ptprj), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ptprj
• Synonyms: AI450271; BET; Byp; CD148; DEP-1; Ptpb2; PTPbeta2; RPTPJ; Scc-1; Scc1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224183 representing NM_001135657
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTATGGATAATGTCTTGGGAGGTTTTGAAATGCCACAAGCCTTGCTGGCCCAGGGTGCAGCTGCCT
  CTGCTGTTCAGACCTCAGATTATAAGGACAGAACACAGCACGGAAGTGGGGGGATCAGAACCATGGGAGA
  TCCAGGACGAAGGGTCCTGTGCGCAGGTGCGGCACCGAATCCTATATTTGACATTGAAGCTGTCGTCAGC
  CCAACTAGTGTGTTATTAACATGGAAGCACAATGACTCAGGCGCTTCAGAATGTAGAATAGAGAATAAGA
  TGGAGAGCAATCTGACGTTTCCTGTTAAAAACCAGACATCATGTAACATTACAGGCTTAAGCCCAGGCAC
  TTCGTATACATTCTCCATCATCTCTGTAACAACCAATGAGACCTTGAACAAAACTATCACAACAGAGCCC
  TGGCCAGTGTCTGATCTCCATGTCACCTCTGTGGGTGTGACACAGGCTCGTCTCACCTGGAGCAATGCAA
  ATGGCACTGCCTCCTACCGGATGCTGATTGAAGAGTTGACCACACATTCCTCAGTCAATATTTCAGGTCT
  GAAGCCGGGGACCAATAATAGCTTCGCTTTCCCAGAATCAAATGAGACACAGGCTGACTTTGCAGTTGCA
  GAGGAGGTCCCGGATGCCAATGGTACCAAGAGAATCCCAGTGACCAACCTATCCCAACTACACAAGAATT
  CTCTTGTCTCTGTGGACCCACCCTCTGGCCAGGATCCCTCCCTCACAGAGATCTTGCTTACTGACCTAAA
  GCCTGATACTCAGTACAATGCCACCATCTATTCTCAAGCAGCAAATGGCACTGAAGGACAGCCCAGGAAC
  AAAGTGTTTAAAACAAATTCCACCCAGGTTTCTGACGTCCGAGCTATGAACATCAGTGCCTCAAGCATGA
  CCCTGACCTGGAAAAGCAATTACGATGGGTCCCGTACTTCAATTGTCTACAAAATACACGTGGCTGGGGG
  GACCCACTCCGTCAACCAAACTGTCAATAAGACTGAGGCCATCATCCTCGGACTCAGCTCCAGCACCTTG
  TACAACATCACAGTTCATCCTTTCCTGGGTCAGACGGAGGGCACACCAGGCTTCCTCCAAGTGTACACTT
  CCCCCGATCAGGTCTCTGACTTCCGAGTGACAAATGTCAGCACAAGGGCAATTGGTTTGGCTTGGAGGAG
  CAATGACTCCAAGTCCTTCGAGATTTTCATCAAGCAGGACGGAGGTGAGAAGCATCGAAATGCTTCGACG
  GGAAACCAGAGCTATATGGTTGAAGATTTAAAGCCTGGAACCAGTTACCATTTTGAGATAATTCCACGAG
  GACCAGACGGGACAGAAGGGCTGTCCAGTACAGTGAATGGGAGCACTGACCCCAGTGCTGTGACTGACAT
  CCGGGTGGTCAACATTAGCACCACTGAAATGCAGTTGGAGTGGCAGAATACGGACGATGCCTCTGGATAC
  ACTTACCATTTAGTTCTAGAGTCTAAAAGTGGCTCCATCATCAGGACCAACAGTTCTCAGAAGTGGATCA
  CAGTAGGGAGCCTCACCCCAGGCACCTTATACAATGTCACAATCTTTCCAGAAGTGGACCAGATCCAGGG
  AATCTCCAACTCCATTACCCAGTACACACGGCCCAGCAGTGTGTCCCACATTGAAGTAAACACCACCACC
  ACCACGGCAGCCATCCGATGGAAGAACGAGGACGCAGCCTCTGCTTCCTATGCCTACTCCGTCCTTATCT
  TGAAGACTGGAGATGGCAGCAATGTAACCAGCAACTTCACAAAAGACCCTTCTATTCTAATCCCTGAGTT
  AATCCCTGGTGTCTCTTACACAGTGAAGATCCTTACACAAGTTGGGGATGGTACAACATCACTGGTACCT
  GGTTGGAATCTGTTCTGTACGGAACCTGAACCAGTGACCTCCTTCCACTGTGAAGTGGTCCCTAAGGAGC
  CAGCATTGGTTCTCAAGTGGGCCTGCCCCTTTGGCATGTACACAGGCTTCGAGCTGGGGGTCAGGAGTGA
  TTCCTGGGACAATATGACACGCCTAGAGAACTGCACTTCGGATGATGACACAGAGTGCAGGACGGAAGTC
  GCCTATTTGAATTTTTCTACCTCGTACAACATCAGCATCGCCACCTTGTCATGTGGGAAGATGGCGCTTC
  CCGCCCAGAACATCTGCACCACTGGCATCACAGACCCACCTACTCCGGATGGATCCCCTAATATTACATC
  GGTCAGTCACAATTCAGTAAAGGTTAAGTTCAGCGGGTTTGAAGCCAGCCACGGACCTATCAAAGCCTAT
  GCTGTCATCCTCACCACCGGGGAAGCTGCCCAACCTTCTGCAGATGTTTTGAAGTACACGTATGAGGATT
  TCAAAAGGGGAGCCTCGGATACTTATGTCACATACCTCATAAGAATAGAAGAGAAGGGACAGTCTCAGGG
  CTTGTCTGAAGTCTTGAACTATGAAATTGATGTGGGGAACCAATCCACTACCCTCGGCTACTACAACGGG
  AGGCTGGAGCCTCTGGGCTCCTACCGGGCTTGTGTTGCTGGCTTTACCAATATTACCTACAACCTTCAGA
  ATGACGGCCTCATCAATGGGGATGAGAGCTATGTGTCTTTCAGTCCATATTCAGAGGCCGTGTTCTTGCC
  CCAGGACCCAGGTGTCATCTGCGGAGCAGTGTTTGGATGTATCTTTGGTGCCCTGGCCATCACAGCTGTG
  GGAGGCTTCATCTTCTGGAGAAAGAAAAGGACAGATGCCAAGAATAATGAAGTGTCCTTTTCTCAAATTA
  AACCTAAAAAATCCAAGTTAATCCGAGTGGAGAATTTTGAGGCCTACTTTAAGAAGCAGCAAGCTGACTC
  TAACTGTGGGTTTGCAGAGGAATATGAGGACCTGAAGCTGATTGGGATAAGTTTACCTAAATACACAGCT
  GAGATAGCCGAGAACAGAGGGAAGAACCGCTACAACAATGTTCTGCCCTATGATATTTCTCGAGTCAAAC
  TTTCAGTCCAGACCCATTCGACAGATGACTACATCAATGCCAACTATATGCCTGGCTACCATTCCAAGAA
  AGATTTCATTGCCACACAAGGACCTTTACCCAACACTTTGAAAGATTTCTGGCGTATGGTTTGGGAGAAA
  AACGTATATGCCATTGTTATGTTGACCAAATGCGTGGAGCAGGGAAGGACCAAATGTGAGGAGTACTGGC
  CTTCCAAGCAGGCTCAGGACTACGGGGACATAACTGTGGCGATGACATCAGAAGTCGTTCTTCCAGAATG
  GACCATCAGAGATTTTGTGGTGAAAAATATGCAGAATAGCGAGAGCCATCCTCTGCGGCAGTTCCATTTC
  ACCTCCTGGCCTGACCACGGTGTTCCTGACACCACTGACCTGCTCATCAACTTTCGGTACCTGGTCCGGG
  ATTACATGAAGCAGATACCCCCCGAGTCACCAATTCTGGTGCATTGCAGTGCTGGGGTTGGAAGGACGGG
  CACTTTCATCGCCATCGATCGCCTGATCTATCAGATAGAGAATGAGAACACCGTGGACGTGTATGGGATT
  GTCTATGACCTTCGGATGCACAGGCCTCTGATGGTGCAGACAGAGGACCAGTATGTTTTCCTCAATCAGT
  GTGTTTTGGATATTATCAGAGCCCAGAAAGACTCAAAAGTTGATCTCATCTATCAGAACACAACGGCAAT
  GACAATCTATGAAAACCTCGAGCCAAGTTCCTTGAATGTGACTATGTTGCTTCATCCACAGCTGAACGAT
  TTTGGATGTTGGGTTCTAGGTCCTGGCTGTTGCTGGTCTGCTAGGATCCAGGGCCTTGTTGACATCTGGG
  AAGATGTAAATTGTCCCGCTGAAAGCCTCAGTTTTAGATGTGGCCACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001135657
• Insert Size: 3900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001135657.1, NP_001129129.1
• RefSeq Size: 4201 bp
• RefSeq ORF: 3900 bp
• Locus ID: 19271
• Cytogenetics: 2 50.19 cM
• Gene Summary: Tyrosine phosphatase which dephosphorylates or contributes to the dephosphorylation of CTNND1, FLT3, PDGFRB, MET, RET, KDR, LYN, SRC, MAPK1, MAPK3, EGFR, TJP1, OCLN, PIK3R1 and PIK3R2. Plays a role in cell adhesion, migration, proliferation and differentiation. Involved in vascular development. May be involved in the mechanism of contact inhibition of cell growth. Regulator of macrophage adhesion and spreading. Positively affects cell-matrix adhesion. Positive regulator of platelet activation and thrombosis. Negative regulator of cell proliferation. Negative regulator of PDGF-stimulated cell migration; through dephosphorylation of PDGFR. Positive regulator of endothelial cell survival, as well as of VEGF-induced SRC and AKT activation; through KDR dephosphorylation. Negative regulator of EGFR signaling pathway; through EGFR dephosphorylation. Enhances the barrier function of epithelial junctions during reassembly. Negatively regulates T-cell receptor (TCR) signaling. Upon T-cell TCR activation, it is up-regulated and excluded from the immunological synapses, while upon T-cell-antigen presenting cells (APC) disengagement, it is no longer excluded and can dephosphorylate PLCG1 and LAT to down-regulate prolongation of signaling.[UniProtKB/Swiss-Prot Function]