Ube2o (NM_173755) Mouse Untagged Clone

CAT#: MC224173

Ube2o (untagged) - Mouse ubiquitin-conjugating enzyme E2O (Ube2o), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ube2o
• Synonyms: 9630022H21; B230113M03Rik; E2-230K; mKIAA1734
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224173 representing NM_173755
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGATCCCGCAGCGCCCGCACCCGCCCAGGCCCAGGCCGCCGCCGCCCCGACCCCGGCCGCAGCCC
  CCGCCGCCGCCGCCCCGCCGCCCGCGCCCGCTACGGACTCGGCCTCAGGGCCGTCCTCCGACTCCGGCCC
  CGAAGCAGGCTCACAGCGCCTGCTGTTCTCCCACGACCTGGTGTCCGGCCGTTACCGCGGCTCGGTGCAC
  TTCGGGCTGGTACGCCTCATCCACGGCGAGGACTCGGACTCGGAGGGCGACGATGACGGCCGCGGCAGCT
  CGGGCTGCTCCGAGGCCGGGGGCGCGGGCCACGAGGAGGGCCGGGCCAGCCCTCTGCGGCGCGGCTACGT
  GCGCGTCCAGTGGTACCCGGAGGGCGTCAAGCAGCATGTGAAGGAGACCAAGCTGAAACTGGAGGACCGC
  TCTGTGGTGCCCCGGGATGTGGTTCGACACATGCGCTCAACTGACAGCCAGTGTGGCACGGTGATAGATG
  TCAACATTGACTGTGCCGTCAAGCTCATCGGCACCAACTGCATCATCTACCCTGTCAACAGCAAGGACCT
  CCAGCACATCTGGCCCTTCATGTATGGAGACTATATCGCCTATGACTGCTGGCTGGGGAAGGTCTATGAC
  TTGAAGAACCAGATCATCCTCAAGTTGTCTAATGGTGCCAGGTGCTCCATGAACACAGAAGATGGTGCCA
  AACTCTACGATGTCTGCCCTCACGTCAGCGACTCGGGTCTCTTCTTTGATGACTCTTATGGCTTCTACCC
  AGGCCAAGTCCTTATTGGCCCAGCCAAGATCTTCTCCAGTGTCCAGTGGCTCTCTGGGGTCAAGCCTGTA
  CTCAGCACCAAGAGCAAGTTCCGTGTGGTGGTGGAGGAGGTGCAGGTTGTAGAACTGAAAGTCACGTGGA
  TTACCAAGAGCTTCTGCCCCGGCGGCACGGACAGCGTTAGCCCCCCACCTTCCATCATCACTCAGGAAAA
  CCTAGGCAGGGTGAAGCGTCTCGGATGCTTTGACCATGCTCAGCGGCAGCTTGGGGAACGCTGTCTGTAT
  GTCTTCCCAGCCAAGGTAGAGCCGGCCAAGATTGCCTGGGAATGTCCAGAAAAAAACTGCGCCCAGGGGG
  AGGGCTCTATGGCCAAGAAGGTGAAGCGTCTGTTGAAGAAGCAAGTTGTGAGAATCATGTCCTGCACCCC
  AGATACCCAGTGTCCTAGGGACCATTCCATGGAAGACCCAGACAAGAAAGGGGAAGCCAGAGCCGGGAGT
  GAAATAGGGTCTGCTAGCCCGGAGGAGCAACCTGATGGATCAGCCAGCCCCGTGGAGATGCAGGATGAGG
  GGTCGGAGGAGCTTCAGGAGACATGCGAGCCACTGCCCCCCTTCCTCTTGAAGGAGGGTGGAGATGACGG
  GCTGCACTCGGCAGAGCAGGACGCAGATGATGAGGCGGCTGACGATACAGATGACACCAGCTCTGTGACC
  TCCTCCGCCAGCTCTACTACCTCCTCCCAGAGTGGCAGTGGCACTGGTCGTAAAAAGAGCATTCCCTTGT
  CCATCAAAAACTTGAAGCGAAAACACAAGAGGAAGAAGAATAAGGTCACCCGTGACTTCAAGCCTGGAGA
  CAGGGTGGCAGTGGAGGTTGTGACCACGATGACCTCAGCTGATGTGATGTGGCAAGATGGCTCTGTGGAA
  TGCAACATCCGCTCCAACGACCTTTTCCCTGTGCACCACCTGGACAACAATGAGTTCTGCCCTGGGGACT
  TCGTGGTGGACAAGCGAGTCCAGAGCTGCCCAGACCCTGCTGTCTATGGTGTGGTGCAGTCTGGGGATCA
  TGTTGGCCGTACCTGCATGGTGAAGTGGTTCAAGCTGCGACCAAGTGGGGATGACGTGGAGCTCATTGGA
  GAAGAGGAGGACGTGAGCGTCTATGACATTGCTGACCACCCTGACTTTCGCTTCCGCACAACTGACATTG
  TCATCCGCATTGGTAACACGGAGGATGGGGCTCTACCCAAGGAGGATGAGCCCTCAGTGGGCCAGGTGGC
  CCGAGTGGATGTCAGCAGCAAAGTGGAAGTGGTGTGGGCTGACAACTCGAAAACCATCATCCTGCCACAG
  CACTTATACAACATCGAGTCTGAGATTGAGGAGTCTGACTACGACTCCGTGGAAGGCAGCTCCAGTGGGG
  CCTCCTCGGATGAGTGGGAGGACGACAGCGACAGCTGGGAGACCGACAACGGGCTCGTGGACGATGAGCA
  CCCCAAGATAGAGGAGCTGGCCGCCATCTTGCCTGCCGAGCAGCCCACTGCCCCCGAGGAGGACAAGGGC
  GTGGTCATCAGTGAGGAGGCGGCCACGGCTGCCATCCAGGGCGCCGTGGCCATGGCTGCCCCCGTGGCAG
  GGCTGATGGAGAAAGCTGGGAAGGACGGGCCTCCCAAGAGTTTCCGGGAGCTGAAGGAGGCCATCAAGAT
  CCTGGAGAGCCTCAAGAACATGACTGTGGAGCAGCTGCTCACAGGCTCGCCCACCTCGCCCACCGTGGAG
  CCTGAGAAGCCAACCCGAGAGAAGAAGTTTTTGGACGATATCAAGAAGCTACAGGAGAACCTCAAGAAGA
  CCCTGGACAATGTGGCCATTGCTGAGGAGGAGAAGATGGAGGCAGTACCCGACACAGAGCGCAAGGAGGA
  GAAGCCTGAGGTGCAGTCCCCAGTGAAAGCCGAGTGGCCCAGTGAGACACCTGTGCTCTGTCAGCAGTGC
  GGTGGCAGGCCTGGCGTCACCTTCACCAGCGCCAAGGGCGAGGTCTTCTCTGTGCTGGAGTTTGCACCCT
  CAAACCACTCTTTCAAGAAGATTGAGTTCCAGCCCCCGGAAGCCAAGAAGTTCTTCAGCACAGTGCGGAA
  GGAGATGGCTCTGCTGGCCACCTCGCTGCCCGATGGCATCATGGTCAAGACTTTTGAGGACAGAATGGAC
  CTCTTCTCTGCACTGATCAAGGGCCCCACTCGGACTCCCTATGAAGACGGCCTCTACCTATTTGACATCC
  AGCTTCCCAACATCTACCCTGCGGTGCCCCCCCACTTTTGCTACCTCTCCCAGTGCAGTGGCCGCCTGAA
  CCCCAACCTGTATGACAATGGGAAAGTGTGTGTCAGCCTCCTAGGTACCTGGATTGGAAAGGGGACAGAG
  AGGTGGACAAGCAAGTCTAGCCTTCTCCAGGTGCTCATCTCCATCCAAGGTCTGATCCTGGTGAATGAAC
  CATACTACAATGAAGCTGGCTTTGACAGTGACCGGGGCCTGCAGGAAGGCTATGAGAACAGTCGCTGCTA
  CAACGAGATGGCATTAATCCGTGTGGTGCAGTCCATGACCCAGTTGGTGCGACGACCTCCTGAGGTCTTC
  GAACAGGAGATTCGGCAGCACTTCAGTGTGGGTGGCTGGCGGCTGGTGAACCGAATTGAGTCCTGGCTGG
  AAACCCATGCCATGCAGGAGAGGGCCCAGGTGATGCCCAATGGGGCCCTTAAAGACAGCAGCTCTCTGGA
  GCCCATGGCTGCAGCTGAACTCTCAGACTCCGGCCGTGAAGAGCCTGAAGATGTTGGGATGGCCCCTGGG
  GAGGCGTCCCAGGGCTCAGACTCAGAGGGTGGAGCCCAGGGCCCTGCCTCAGCCAGCAGAGACCACACAG
  AGCAGACTGAGACAGCCCCTGATGCATCCGCACCTCCCAGCGTTAGACCAAAGAGGAGGAGGAAGAGCTA
  CCGCAGCTTCCTGCCTGAGAAGAGCGGTTACCCTGACATCGGCTTCCCCCTCTTCCCACTCTCCAAGGGT
  TTCATCAAGAGCATCCGGGGGGTCCTGACACAGTTCCGTGCTGCCCTGCTGGAGGCGGGCATGCCCGAAA
  GCACAGAGGACAAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_173755
• Insert Size: 3867 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173755.3, NP_776116.2
• RefSeq Size: 5192 bp
• RefSeq ORF: 3867 bp
• Locus ID: 217342
• UniProt ID: Q6ZPJ3
• Cytogenetics: 11 E2
• Gene Summary: E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins. Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity. Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis. Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location. Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly.[UniProtKB/Swiss-Prot Function]