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Crb2 (NM_001163566) Mouse Untagged Clone

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SKU
MC224155
Crb2 (untagged) - Mouse crumbs homolog 2 (Drosophila) (Crb2), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $1,786.00
7 Weeks*
Bulk Requests & Clone Modifications

Frequently Bought Together (2)

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Other products for "Crb2 or NM_001163566"

Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Crb2
Synonyms 5930402A21; BC043114
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC224155 representing NM_001163566
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCTGGTGGGGCCTAGGATTTGGGGCCCTAGACGAGATATTTACCCCCTGTTGCTGCTACTGCTAC
TGCTGCTGCTGCTGCTGCTGCCTTGGGTCCCGGCTGGGCTGGTTCCTCCAGAGACTCCAAGTGTCTGTGC
CTCAGACCCATGTGCTCCAGGGACCAAGTGCCAGGCTACAGAAAGTGGTGGCTATACCTGTGAGCCCTCA
GAGCTTGGAGGCTGTGCTACTCAGCCATGCCACCACGGGGCACTGTGTGTGCCCCAGGGCCCAGATCCTA
ACAGCTTCCGTTGCTACTGTGTGCCTGGATTCCAGGGACCCCACTGTGAACTGGACATCGATGAGTGTGC
GTCCCGGCCTTGCCAGCATGGGGGCACCTGCCAAAATCTGGCAGATCACTACGAGTGCCACTGTCCCCTG
GGATATGCAGGCGTGACCTGTGAGGCCGAGGTGGACGAGTGCTCGTCAGCGCCCTGCCTGCACGGAGGCT
CGTGCCTGGACGGTGTGGGTTCCTACCGCTGCGTGTGCGCGCCGGGATACGCCGGTGCGAACTGTCAGCT
GGACGTGGACGAGTGCCAGAGCCAGCCGTGCGCGCACGGTGGCGTGTGTCACGACCTGGTCAACGGTTTC
CGGTGCGACTGCGCGGACACGGGTTACGAAGGCGCGCGCTGCGAGCAGGAGGTGCTGGAGTGCGCCTCTG
CGCCCTGCGCGCACAACGCGTCCTGCCTCGACGGCTTCCGGAGCTTCCGCTGCCTCTGCTGGCCAGGCTT
CAGTGGAGAGCGGTGCGAAGTGGATGAGGACGAATGTGCATCGGGCCCCTGTCAAAATGGGGGCCAGTGC
TTGCAGCGCTCTGACCCGACCTTGTACGGGGGTGTTCAGGCCATCTTCCCGGGAGCCTTCAGCTTCAGCC
ACGCCGCTGGCTTCCTTTGCAGCTGTCCTCTGGGCTTTGCTGGGAATGACTGCAGCATGGATGTGGATGA
GTGTGCCTCAGGGCCGTGCCTCAATGGAGGTAGCTGCCAGGACCTGCCCAATGGTTTCCAGTGCTACTGC
CAGGATGGATACACAGGCCTGACATGTCAGGAAGATATGGATGAATGCCAGTCGGAACCATGCCTGCATG
GTGGAACCTGCAGCGACACTGTAGCAGGCTACATCTGCCAGTGCCCTGAGGCCTGGGGTGGACATGACTG
TTCTGTGCAGCTCACAGGCTGCCAGGGCCATACCTGCCCACTGGCTGCCACCTGCATCCCCACCTTCAAG
TCTGGCCTCCACGGTTACTTCTGCCGCTGCCCACCGGGGACCTATGGGCCTTTCTGTGGCCAGAATACGA
CCTTCTCGGTTGTGTCTGGGAGTTCTGTGTGGGGATTGGTGCCAGCTGCTGCCTCCCTGGGTCTGGCACT
GAGATTTCGTACCACCCTGCTTGCCGGAACCCTGGCTACTCTCAAAGACACTCGGGACAGCTTGGAGCTG
GTTCTGGTGGGGGCCGTGCTCCAAGCCACACTCTCGAGACATGGCACTGCGGTGCTCATCCTTACACTGC
CTGACCTAGCCTTAAATGATGGGCATTGGCACCAGGTGGAAGTGACGCTCCACCTGGGAACCCTGGAGCT
GCGGCTCTGGCACGAGGGCTGCCCTGGCCAGCTCTGTGTGGCCTCTGGTCCGGTGGCTACAGGCCCTACA
GCCTCGGTGGCCTCTGGGCCTCCGGGATCCTACTCCATCTATCTGGGCGGCGGGGTCTTTGCCGGCTGCT
TCCAGGATGTGCGTGTGGAAGGGCACCTTCTGCTGCCTGAGGAGCTCAAGGGAACTGTGCTTCTGGGTTG
TGAGCGCAGGGAACCTTGCCAGCCTCTGCCTTGTGCCCATGGAGGGGCCTGCGTGGACCTGTGGACTCAC
TTCCGCTGCGACTGCCCGAGACCTTACCGTGGAGCCACATGCACTGATGAGGTTCCTGCTGCCACTTTTG
GCTTGGGTGGAGCCACGAGCTCAGCCTCTTTTCTGCTCCACCAACTAGGCCCAAACCTCACCGTGTCATT
TTTCCTCCGCACCCGCGAACCTGCCGGCCTGTTGCTCCAGTTTGCCAATGATTCAGTTGCGAGCCTGACT
GTGTTCCTGAGTGAGGGCCAGATCCGGGCTGAGGGGCTGGGTCACCCTGCTGTGGTCCTCCCTGGGCGCT
GGGATGATGGACTCCCCCACTTGGTGATGCTCAGCTTTGGGCCTGACCAGCTGCAGGACCTGGGCCAGCG
GCTGTATGTGGGTGGGAGGTTCTACCCTGATGACACCCAGCTCTGGGGTGGGCCCTTCCGAGGCTGTCTC
CAGGACCTACAACTCAACAGCATCCACCTCCCCTTCTTCTCTTCCCCGATGGAGAACTCAAGTTGGCCCA
GTGAACTGGAAGCTGGCCAGTCCTCCAACCTCACCCAGGGTTGTGTCTCTGAGGACACGTGCAATCCCAA
TCCCTGTTTCAATGGTGGCACGTGCCACGTCACCTGGAATGACTTCTACTGCACCTGCTCCGAGAACTTC
ACGGGGCCCACCTGTGCCCAGCAGCGATGGTGCCCCAGGCAGCCATGCCTGCCTCCTGCCACCTGTGAGG
AGGTTCCAGATGGCTTTGTGTGTGTGGCCGAGGCCACGTTCCGCGAGGGCCCTCCTGCTGTGTTCACAGG
CCACAACGTGTCCTCATCGCTCAGCGGGCTCACCCTGGCCTTCCGCACGCGCGACTCCGAGGCTGGGCTA
CTGCGCGCCGTCTCCGCCGCAGGTGCCCACTCCAATATCTGGTTGGCGGTGCGCAACGGCTCGCTGGCAG
GAGATGTGGCGGGTTCGGTGCTGCCCGCGCCCGGGCCGCGCGTGGCCGACGGCGCCTGGCATCGCGTGCG
CCTAGCCCGGGAGTTCCCACAGGCCGCTGCCTCGCGCTGGCTGCTGTGGCTGGACGGCGCGGCGACACCC
GTGGCCTTGCACGGCTTGGGCGGCGACCTGGGCTTTCTGCAGGGTCCGGGTGCAGTGCCTCTGCTACTGG
CTGAGAACTTCACGGGCTGCCTGGGCCGCGTGGCACTTGGCGACTTCCCGTTACCCTTGGCGCCACCGCG
GTCTGGCACGGTGTCTGGGGCGCGCGAGCACTTCGTGGCCTGGCCCGGGTCTCCGGCCGTGAGTCTCGGC
TGCCGTGGCGGACCCGTATGTTCACCCTCGCCCTGCCTGCACGGTGGCGCCTGCCGCGACCTCTTCGACG
CCTTTGCTTGCTCCTGCGGCCCCGCCTGGGAAGGACCCCGCTGCGAGATCCGCGCTGACCCATGTCGCTC
CACTCCCTGTGTCCGGGGCCAATGCCATGCGCGCCCCGACGGCCGCTTCGAGTGTCGCTGCCCTCCGGGC
TTCTCCGGCCCGCGCTGCAGGTTGCCTGTCCTGCCTCAGGGATGCAACCTCAACTCCACATGCAAGGATG
GCGCCCCTTGTGAAGGCGGCCCCTTAGGCACCAACTGCAGTTGCCAGGAAGGTCTTGCTGGCCTCAGATG
TCAGAGTCTCGACAAACCCTGTGAAGCCAGCCCTTGCTTGAATGGGGGCACTTGCCGGGTGGCCAGTGGC
ATATTTGAATGTACTTGCAGTGCAGGATTCTCTGGCCAGTTCTGTGAAGTGGTGAAAACCCTACCTCTGC
CTCTGCCGTTCCCACTGCTGGAGGTAGCAGTGCCTGCAGCCTGTGCCTGCCTCCTCCTTCTCCTCCTGGG
CCTCCTCTCAGGAATCCTGGCTGCCAGAAAGCGCCGCCAGTCTGAGGGCACTTACAGCCCAAGTCAGCAG
GAGGTGGCTGGGGCTCGTCTGGAGATGGACAGTGTCCTCAAGGTGCCACCAGAGGAGAGACTTATTTAG

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-RsrII |
ACCN NM_001163566
Insert Size 3849 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001163566.1, NP_001157038.1
RefSeq Size 6393 bp
RefSeq ORF 3849 bp
Locus ID 241324
UniProt ID Q80YA8
Cytogenetics 2 B
Summary Apical polarity protein that plays a central role during the epithelial-to-mesenchymal transition (EMT) at gastrulation, when newly specified mesodermal cells move inside the embryo (PubMed:26496195, PubMed:27870829). Acts by promoting cell ingression, the process by which cells leave the epithelial epiblast and move inside the embryo to form a new tissue layer (PubMed:27870829). The anisotropic distribution of CRB2 and MYH10/myosin-IIB at cell edges define which cells will ingress: cells with high apical CRB2 are probably extruded from the epiblast by neighboring cells with high levels of apical MYH10/myosin-IIB (PubMed:27870829). Also required for maintenance of the apical polarity complex during development of the cortex.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001163566" in other vectors (4)
SKU Description Size Price
MG222655 Crb2 (tGFP-tagged) - Mouse crumbs homolog 2 (Drosophila) (Crb2), (10ug) 10 ug
$1,874.00
MR222655 Crb2 (Myc-DDK-tagged) - Mouse crumbs homolog 2 (Drosophila) (Crb2) 10 ug
$1,674.00
MR222655L3 Lenti ORF clone of Crb2 (Myc-DDK-tagged) - Mouse crumbs homolog 2 (Drosophila) (Crb2) 10 ug
$1,974.00
MR222655L4 Lenti ORF clone of Crb2 (mGFP-tagged) - Mouse crumbs homolog 2 (Drosophila) (Crb2) 10 ug
$1,974.00

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