Gigyf2 (NM_001110212) Mouse Untagged Clone

CAT#: MC224153

Gigyf2 (untagged) - Mouse GRB10 interacting GYF protein 2 (Gigyf2), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gigyf2
• Synonyms: 2610016F01Rik; A830080H02Rik; AI852361; AW259676; BC006835; mKIAA0642; Tnrc15
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224153 representing NM_001110212
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCAGAAACACAGACACTAAACTTTGGGCCTGAATGGCTCCGTGCTCTGTCCAGTGGTGGGAGTA
  TCACGTCCCCTCCTCTTTCTCCGGCCCTGCCAAAGTATAAATTAGCAGATTATCGATATGGCCGAGAAGA
  AATGTTAGCACTTTTCCTTAAAGACTACAAGATACCCTTTGATCTTCTGGAGAAAGAATTCCTGCCTATC
  CTACAGGAGGAGCCCTTGCCACCATTGGCTCTAGTACCCTTTACAGAAGAAGAGCAGAGAAACTTTTCCA
  TGTCTGTAAATAGTGCCGCTGTCCTGCGATTGACAGGACGAGGAGGAGGAGGAGGAACAGTGGTGGGGGC
  TCCTAGAGGTCGAAGTTCTTCAAGAGGGCGAGGCAGAGGTAGAGGTGAATGTGGTTTCTACCAAAGAAGT
  TTTGATGAAGTAGAGGGTGTGTTTGGGCGAGGAGGTGGCAGGGAAATGCATAGATCACAGAGCTGGGAAG
  AAAGGGGTGACAGGCGCTTTGAAAAACCAGGAAGAAAAGATGTAGGGAGACCAAATTTTGAGGAAAGTGG
  ACCAACGTCTGTGGGGAGAAAGCATGAATTTATACGGTCAGAAAGTGAAAACTGGCGAATCTTTAGAGAG
  GAACAGAATGGAGAAGATGAAGATGGAGGTTGGCGGTTAGCTGGATCAAGAAGGGATGGAGAGAGATGGC
  GGCCTCACAGTCCTGGCTGGCGGGAACACATGGAACGCCGTCGGAGGTTTGAGTTTGATTTTCGAGACCG
  AGATGATGAGCGTGGTTACCGAAGGGTGCGCTCTGGCAGTGGGAGCATAGATGACGACAGGGATAGCTTG
  CCGGAGTGGTGCTTAGAGGATGCTGAGGAAGAAATGGGCACGTTTGACTCTTCTGGGGCATTCCTCTCTC
  TAAAAAAAGTGCAGAAAGAACCTATTCCTGAAGAGCAGGAAATGGACTTTAGGCCTGTGGAGGAGGGAGA
  GGAGCGCTCTGACTCTGACAGCAGCCATAACGAAGAGGCCAAAGAACCGGATAAGACAAACCGAAGAGAG
  GGGGAGAAAACAGACAGAGCAGGGGCTGAAGCTAGTGAGGAAGTACCTCAGACGTCATTGTCATCTGCTA
  GACCAGGTACCCCTTCAGATCATCAGCCTCAAGAAGCCACACAGTTTGAGAGGAAAGATGAACCCAAAGC
  TGAACAAGTGGAAAAGGCTGAAGAAGAGAATCGGTCAGAAAATAGTCTCTCAGCCAAGGTCCCCAGCAGA
  GGGGATGAAACGGTGCCTGCCTCCCAGCAACCCTCCACACCGCTTCCTCCAGACACAGCCTCTCCTCTCC
  TCATCCTGTCGCCTCCTGTTCCTACTCCTAGTTCTGCCTCTCGGCCAGTTGAGACAGCAGCCGTCGAAGC
  TCCTGGTATGAGCAGTGTTTCCACAGAGCCAGATGATGAAGAGGGCCTCAAACACTTGGAACAGCAAGCT
  GAGAAGATGGTGGCTTATCTCCAAGACAGTGCACTAGACGACGAAAGATTGACATCAAAACTGCAAGAGC
  ACCGAGCTAAAGGAGTGTCAATCCCACTGATGCATGAAGCAATGCAGAAGTGGTATTACAAAGACCCTCA
  GGGGGAGATTCAAGGTCCCTTTAACAATCAGGAGATGGCCGAGTGGTTTCAGGCGGGCTATTTTACTATG
  TCTTTGCTGGTGAAGAGAGCATGTGATGAAAGCTTCCAGCCCCTTGGTGATATCATGAAAATGTGGGGAA
  GGGTTCCTTTCTCTCCAGGCCCAGCTCCCCCTCCTCATATGGGAGAATTGGACCAGGAACGCCTGACCAG
  ACAGCAGGAACTCACTGCCTTATACCAGATGCAGCACCTCCAGTACCAGCAGTTCTTAATCCAACAACAG
  TATGCACAGGTTTTGGCTCAACAGCAGAAAGCAGCTTTGTCATCTCAGCAGCAACAACAATTGGCTCTTC
  TTCTGCAGCAGTTCCAGGCTCTGAAGATGAGAATGTCTGATCAGAACATCATTCCCTCAGTAACTAGGTC
  TGTGTCAGTGCCAGATACTGGCTCTATCTGGGAGCTTCAGCCAGCAGCCTCACAGCCTGCAGTTTGGGAA
  GGTGGTAGTGTATGGGATCTTCCCCTGGACACCACAGCACCAGGTCCTTCCCTGGAACAGCTTCAGCAGC
  TAGAGAAGGCCAAAGCTGCAAAGTTAGAACAGGAGAGAAGAGAGGCAGAAATGAGGGCAAAAAGGGAAGA
  GGAGGAGCGAAAGAGGCAAGAAGAACTCCGAAGACAACAGGAGGAAATCCTTCGGAGGCAGCAGGAGGAA
  GAAAGGAAAAGACGGGAAGAAGAGGAACTTGCTCGAAGGAAGCAGGAGGAGGCTCTGCGGCGCCAACGGG
  AGCAAGAGATTGCGCTAAGGCGGCAGCGAGAAGAGGAGGAAAGACAGCAGCAGGAAGAAGCTCTTAGACG
  ATTGGAGGAGCGGAGGAGAGAAGAGGAGGAAAGGCGGAAGCAGGAAGAACTGCTGCGCAAGCAGGAAGAG
  GAGGCTGCCAAATGGGCCCGGGAAGAGGAAGAAGCCCAGCGCCGACTGGAAGAGAACCGGCTGCGCATGG
  AGGAGGAGGCTGCCAGACTTCGGCATGAAGAAGAAGAAAGAAAACGGAAGGAATTGGAGCTGCAGAGGCA
  GAAGGATCTGATGCGTCAGAGACAGCAGCAGCAAGAGGCTCTCCGAAGGCTGCAGCAGCAACAGCAGCAG
  CAACAGCTTGCACAAATGAAGCTTCCCTCTTCGTCAACGTGGGGTCAGCAGTCTAACACAGCAACATGTC
  AGTCCCAGGCCACGCTGTCATTGGCTGAGATCCAGAAGCTGGAAGAAGAACGAGAACGCCAGCTTCGTGA
  AGAGCAAAGACGTCAGCAGAGGGAATTGATGAAAGCTCTCCAGCAGCAGCAGCAGCAGCAGCAACAACAA
  AAACTCTCAGGTTGGGGGAATGTCAGCAAACCTGCAGGTACCACCAAGTCTCTACTAGAGATCCAGCAGG
  AAGAGGCCAGGCAGATGCAGAAGCAGCAGCAGCAGCAACAACAACAACAACAGCAACACCAGCAATCAAA
  CAGAGCCCGGAACAGCACACATTCCAACCTGCATACCAGCCTTGGGAATTCTGTATGGGGCTCTATAAAC
  ACAGGTCCTTCTAACCAGTGGGCGTCTGAGCTAGTCAGTAGTATCTGGAGTAATGCAGACACCAAAAACT
  CCAACATGGGGTTTTGGGATGATGCAGTGAAAGAGGTGGGACCTAGAAATTCAACAAATAAAAATAAAAA
  CAACGCCAGTCTCAGTAAATCTGTAGGTGTGTCTAACCGGCAGAATAAGAAAGTAGAAGAAGAAGAAAAG
  TTGCTGAAGCTCTTTCAGGGAGTAAATAAAGCCCAAGATGGATTTACCCAGTGGTGTGAACAGATGCTTC
  ATGCCCTTAATACGGCAAATAACTTGGATGTTCCCACGTTTGTTTCTTTCTTGAAAGAAGTGGAGTCTCC
  TTATGAAGTCCATGACTATACCAGGGCCTATTTAGGAGATACTTCCGAGGCCAAGGAGTTTGCCAAGCAG
  TTCCTTGAACGCCGTGCCAAACAGAAAGTCAACCAGCAGCGTCAGCAGCAGCAGCAGCAGCAGCAGCAGC
  AGGACTCTGTCTGGGGGATGAACCATAGTACACTCCATTCGGTATTTCAGACCAATCAAAGCAACAACCA
  ACAATCAAATTTTGAGGCTGTGCAAAGTGGCAAGAAGAAGAAAAAGCAGAAGATGGTTCGAGCAGATCCC
  AGTTTATTAGGGTTTTCAGTCAACGCCTCGTCAGAGCGACTGAACATGGGTGAGATTGAGACTCTGGATG
  ACTACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001110212
• Insert Size: 3858 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001110212.2, NP_001103682.1
• RefSeq Size: 5725 bp
• RefSeq ORF: 3858 bp
• Locus ID: 227331
• UniProt ID: Q6Y7W8
• Cytogenetics: 1 C5- D
• Gene Summary: Key component of the 4EHP-GYF2 complex, a multiprotein complex that acts as a repressor of translation initiation (PubMed:26763119). In 4EHP-GYF2 the complex, acts as a factor that bridges EIF4E2 to ZFP36/TTP, linking translation repression with mRNA decay (PubMed:26763119). May act cooperatively with GRB10 to regulate tyrosine kinase receptor signaling, including IGF1 and insulin receptors (PubMed:12771153).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate splice site in the coding region compared to variant 1. The resulting protein (isoform b) is shorter but has the same N- and C- termini compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.