Sin3a (NM_001110351) Mouse Untagged Clone

CAT#: MC224129

Sin3a (untagged) - Mouse transcriptional regulator, SIN3A (yeast) (Sin3a), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sin3a
• Synonyms: AW553200; mKIAA4126; mSin3A; Sin3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224129 representing NM_001110351
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCGACGTTTGGATGACCAGGAATCACCAGTGTATGCAGCCCAGCAGCGAAGGATTCCTGGGAGCA
  CAGAGGCTTTTTCTCACCAGCACCGGGTCCTTGCCCCGGCCCCTCCTGTGTATGAAGCAGTGTCTGAGAC
  CATGCAGTCAGCTACAGGCATTCAGTACTCAGTGGCACCCAACTACCAGGTTTCAGCTGTGCCACAAAGT
  TCTGGCAGTCATGGGCCCGCCATAGCAGCAGTTCATAGCAGCCATCATCACCCAACAGCTGTCCAGCCTC
  ATGGAGGCCAGGTGGTCCAGAGCCATGCCCACCCAGCACCACCAGTTGCACCAGTACAGGGACAGCAGCA
  GTTTCAGAGGCTCAAGGTGGAAGACGCCCTGTCCTATCTTGACCAGGTGAAACTGCAGTTCGGTAGTCAG
  CCTCAGGTCTACAATGATTTCCTTGACATCATGAAGGAATTTAAATCTCAGAGCATTGATACTCCAGGAG
  TGATTAGCCGAGTGTCCCAGCTATTTAAAGGCCACCCTGATCTGATCATGGGCTTTAACACCTTCTTGCC
  TCCTGGCTACAAAATTGAGGTGCAGACTAATGACATGGTGAACGTGACAACACCTGGCCAAGTTCATCAG
  ATTCCCACCCATGGCATCCAGCCCCAGCCTCAGCCACCACCTCAGCATCCTTCCCAGCCTTCATCCCAGT
  CAGCTCCCACTCCTGCTCAGCCAGCTCCTCAGCCCACAGCTGCCAAAGTCAGCAAGCCTTCCCAACTACA
  AGCACATACTCCAGCCAGTCAGCAGACTCCCCCACTCCCACCATATGCATCCCCACGTTCTCCACCAGTC
  CAGCCTCACACACCAGTGACAATCTCCTTGGGGACAGCTCCATCTTTGCAAAACAATCAGCCTGTGGAGT
  TTAATCATGCCATCAACTATGTTAATAAGATCAAGAACAGATTCCAGGGCCAACCAGACATCTACAAAGC
  ATTCTTGGAGATTTTGCACACATACCAGAAAGAACAGCGGAATGCCAAGGAAGCTGGAGGAAACTACACT
  CCAGCTTTGACTGAGCAAGAGGTGTATGCCCAGGTGGCTCGACTCTTCAAAAACCAGGAAGATTTGTTGT
  CTGAATTTGGACAGTTCCTGCCAGATGCCAACAGCTCAGTGCTTTTAAGCAAAACAACTGCTGAGAAGGT
  TGATTCTGTGAGAAATGACCATGGAGGCACTGTGAAGAAGCCCCAACTGAATAACAAGCCACAGAGGCCC
  AGTCAGAATGGCTGCCAGATCCGCAGGCACTCTGGAACAGGAGCCACACCTCCAGTGAAGAAAAAACCCA
  AACTGATGAGTCTAAAAGAGTCTTCAATGGCAGATGCCAGCAAGCATGGTGTTGGAACGGAATCATTATT
  TTTTGATAAGGTTCGAAAGGCTCTTCGGAGTGCAGAGGCCTATGAAAACTTCCTTCGTTGCCTTGTTATC
  TTTAATCAGGAGGTGATCTCTCGGGCCGAGCTTGTACAGCTAGTCTCTCCTTTTCTGGGGAAATTCCCTG
  AATTGTTTAATTGGTTTAAAAACTTTTTGGGCTATAAGGAGTCTGTACATCTGGAAAGCTTTCCAAAGGA
  ACGAGCTACAGAAGGCATTGCCATGGAGATAGACTATGCCTCTTGTAAACGACTGGGCTCTAGCTACCGA
  GCCCTACCGAAAAGTTACCAGCAGCCCAAGTGCACGGGACGGACTCCTCTGTGTAAAGAGGTTTTAAATG
  ATACCTGGGTTTCCTTCCCATCTTGGTCTGAAGACTCCACTTTTGTTAGTTCCAAGAAGACTCAGTATGA
  AGAACATATTTACCGTTGTGAAGATGAACGATTTGAGCTTGATGTGGTTCTTGAGACCAATCTTGCAACA
  ATCCGGGTTTTAGAAGCAATACAGAAAAAACTTTCTCGCTTGTCTGCTGAGGAACAAGCCAAATTTCGCT
  TGGATAACACCCTTGGAGGCACGTCCGAAGTCATCCATCGAAAAGCACTCCAGAGGATATATGCTGACAA
  AGCAGCTGATATCATCGATGGCCTGAGGAAGAACCCCTCCATTGCTGTTCCGATTGTCCTTAAAAGGTTG
  AAGATGAAAGAAGAAGAGTGGCGAGAAGCTCAGAGAGGCTTCAACAAGGTCTGGCGAGAGCAAAATGAGA
  AGTACTACTTGAAGTCTCTGGATCACCAAGGCATCAACTTCAAGCAGAACGACACTAAGGTCTTGAGGTC
  TAAGAGCTTACTCAATGAGATCGAGAGCATCTATGACGAGAGGCAAGAGCAGGCTACAGAAGAGAACGCT
  GGTGTACCTGTTGGCCCGCACCTCTCTCTTGCCTATGAAGACAAACAGATACTAGAAGATGCTGCTGCTC
  TGATTATCCACCATGTGAAGAGGCAAACAGGCATTCAGAAAGAGGACAAATACAAAATCAAGCAAATCAT
  GCACCATTTCATTCCTGACCTGCTGTTTGCTCAGAGAGGCGATCTCTCAGATGTGGAAGAAGAGGAGGAG
  GAAGAAATGGATGTGGATGAAGCAACAGGAGCACCTAAGAAGCACAATGGTGTTGGGGGCAGCCCCCCTA
  AGTCCAAGTTGCTATTTAGTAACACAGCAGCTCAAAAGTTAAGAGGGATGGATGAAGTATATAACCTTTT
  CTATGTCAATAACAATTGGTATATCTTTATGCGACTGCATCAAATTCTCTGCTTGAGGCTGCTACGGATT
  TGTTCCCAAGCTGAACGGCAAATTGAAGAAGAAAACCGAGAGAGAGAATGGGAACGGGAGGTGCTAGGCA
  TAAAGCGAGACAAGAGTGATAGTCCTGCCATACAACTACGTCTCAAGGAACCTATGGATGTTGATGTAGA
  AGATTATTACCCAGCTTTCCTGGACATGGTGCGGAGCCTGCTTGATGGCAACATAGACTCATCACAGTAT
  GAAGATTCACTGAGAGAGATGTTCACCATTCATGCCTACATTGCCTTTACTATGGACAAATTAATCCAGA
  GCATCGTCAGACAGCTACAGCACATCGTCAGCGACGAGGTCTGTGTGCAGGTTACTGATCTTTACTTGGC
  AGAAAACAATAACGGAGCCACGGGAGGCCAGCTCAACAGTCAGACTTCAAGGAGCCTTCTGGAGTCAGCA
  TACCAGCGGAAGGCAGAGCAGCTTATGTCAGATGAGAACTGCTTCAAGCTAATGTTCATTCAAAGTCAAG
  GTCAAGTTCAGCTGACTGTTGAGCTCCTGGACACAGAAGAGGAGAACTCAGATGACCCCGTGGAAGCAGA
  GCGTTGGTCAGACTACGTGGAGCGATATATGAGTTCTGATACTACTTCTCCTGAACTTCGAGAACATCTG
  GCACAGAAACCAGTATTTCTCCCAAGGAATTTGCGGCGTATCCGGAAGTGTCAACGTGGTCGAGAGCAAC
  AGGAAAAAGAAGGGAAAGAAGGAAACAGCAAGAAGACCATGGAAAATGTAGAGAGCCTGGATAAGCTGGA
  GTGTAGGTTCAAGCTGAACTCCTATAAGATGGTATATGTGATCAAATCGGAGGACTACATGTACCGGAGA
  ACTGCTCTACTCAGAGCTCATCAGTCCCATGAGCGTGTAAGCAAGCGTCTGCATCAGCGGTTCCAGGCCT
  GGGTGGATAAATGGACCAAGGAGCATGTGCCTCGGGAAATGGCAGCAGAGACCAGCAAATGGCTCATGGG
  TGAGGGGCTCGAGGGCCTGGTACCCTGCACCACCACCTGTGATACAGAGACTCTGCACTTTGTGAGCATT
  AACAAATATCGTGTCAAATACGGCACAGTATTCAAAGCCCCTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001110351
• Insert Size: 3825 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001110351.1, NP_001103821.1
• RefSeq Size: 4998 bp
• RefSeq ORF: 3825 bp
• Locus ID: 20466
• UniProt ID: Q60520
• Cytogenetics: 9 30.89 cM
• Gene Summary: Acts as a transcriptional repressor. Corepressor for REST. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MXD1-MAX heterodimers to repress transcription by tethering SIN3A to DNA. Acts cooperatively with OGT to repress transcription in parallel with histone deacetylation. Involved in the control of the circadian rhythms. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex through histone deacetylation. Cooperates with FOXK1 to regulate cell cycle progression probably by repressing cell cycle inhibitor genes expression (PubMed:22476904). Required for cortical neuron differentiation and callosal axon elongation (PubMed:27399968).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 3, and 4 all encode the same isoform (1).