Abcb4 (NM_008830) Mouse Untagged Clone

CAT#: MC224125

Abcb4 (untagged) - Mouse ATP-binding cassette, sub-family B (MDR/TAP), member 4 (Abcb4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Abcb4
• Synonyms: mdr-2; Mdr2; Pgy-2; Pgy2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224125 representing NM_008830
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCTTGAGGCAGCGAGAAACGGAACAGCACGGCGCCTGGACGGCGACTTTGAACTAGGCAGCATCA
  GCAACCAAGGCAGAGAAAAGAAGAAGAAAGTGAATTTAATTGGCCTGTTGACACTGTTCCGATACTCTGA
  CTGGCAGGATAAATTGTTTATGTTCCTGGGCACCCTCATGGCCATAGCTCATGGATCAGGTCTTCCCCTC
  ATGATGATAGTCTTTGGAGAAATGACAGATAAGTTTGTAGATAATACTGGGAACTTTTCCTTGCCAGTGA
  ATTTTTCATTGTCAATGCTAAATCCAGGAAGAATTTTGGAAGAAGAAATGACTAGATATGCATACTACTA
  TTCGGGACTAGGTGGTGGAGTTCTTGTGGCTGCCTATATCCAAGTCTCATTCTGGACTTTGGCAGCTGGC
  CGACAAATAAAGAAAATCAGGCAAAAATTTTTTCATGCCATCCTCCGACAAGAAATGGGCTGGTTTGACA
  TCAAGGGCACCACTGAACTCAACACACGTCTAACAGATGACGTCTCCAAAATCAGTGAAGGAATTGGTGA
  CAAGGTTGGAATGTTCTTTCAAGCAATAGCCACGTTTTTTGCAGGATTCATAGTGGGGTTCATCAGAGGA
  TGGAAGCTCACCCTCGTGATCATGGCCATCAGCCCCATCCTGGGGCTCTCTACAGCTGTTTGGGCAAAGA
  TACTCTCAACATTTAGTGACAAAGAGCTAGCTGCATATGCAAAAGCAGGTGCCGTGGCTGAAGAGGCTCT
  GGGAGCCATCAGGACCGTGATAGCTTTCGGGGGCCAGAACAAAGAGCTAGAAAGGTATCAGAAACATTTA
  GAAAATGCCAAAAAGATTGGAATTAAAAAGGCTATCTCAGCCAACATCTCCATGGGTATTGCTTTCTTGT
  TAATATATGCATCCTATGCACTGGCCTTCTGGTATGGATCCACTCTGGTTATATCAAAAGAATATACAAT
  TGGAAATGCAATGACAGTCTTCTTCTCAATCCTCATCGGGGCTTTCAGTGTGGGGCAGGCTGCCCCCTGT
  ATTGATGCTTTCGCTAATGCAAGAGGAGCAGCCTATGTGATCTTTGACATTATTGATAATAATCCTAAAA
  TTGACAGTTTTTCAGAGAGAGGACACAAACCAGACAACATCAAAGGAAATTTGGAGTTCAGTGATGTTCA
  TTTTTCCTATCCATCTCGGGCTAATATCAAGATCTTGAAGGGCCTCAACCTGAAGGTGAAGAGTGGACAG
  ACAGTGGCTCTGGTTGGCAACAGCGGCTGTGGAAAAAGCACAACTGTCCAGCTGCTGCAGAGGCTCTACG
  ACCCCACAGAGGGTAAGATTAGCATCGATGGGCAGGATATCAGGAACTTTAACGTCAGGTGTCTAAGGGA
  AATCATTGGTGTGGTAAGTCAAGAGCCCGTGCTGTTCTCTACTACGATCGCTGAAAATATCCGCTATGGC
  CGTGGGAATGTAACGATGGATGAGATTGAGAAAGCCGTCAAAGAGGCCAATGCCTATGACTTCATCATGA
  AACTGCCCCAGAAATTTGACACCCTGGTTGGTGATAGAGGGGCGCAGCTGAGTGGGGGACAGAAACAGAG
  AATCGCCATTGCCCGGGCCCTGGTCCGCAACCCCAAGATCCTCCTGCTGGACGAGGCCACCTCAGCCCTG
  GACACTGAAAGTGAAGCTGAGGTGCAGGCCGCACTGGATAAGGCCAGAGAAGGCCGAACCACCATTGTGA
  TAGCTCACCGATTGTCTACCATCCGGAACGCAGATGTCATCGCTGGGTTTGAGGATGGAGTCATTGTGGA
  ACAAGGAAGTCACAGTGAGCTGATGAAGAAGGAAGGGATCTACTTCAGACTCGTTAACATGCAGACAGCA
  GGAAGCCAGATCCTGTCAGAAGAATTTGAAGTTGAGCTAAGTGACGAAAAGGCTGCTGGAGATGTGGCCC
  CAAATGGCTGGAAAGCACGCATATTTAGGAATTCTACAAAGAAAAGTCTTAAAAGTCCACATCAGAATAG
  GCTGGATGAAGAAACCAATGAACTTGATGCAAACGTGCCACCAGTGTCTTTTCTGAAGGTCTTAAAACTG
  AATAAAACAGAGTGGCCCTACTTTGTGGTGGGAACAGTCTGTGCCATTGCCAATGGAGCCCTCCAGCCGG
  CTTTCTCCATCATCCTGTCTGAGATGATAGCTATCTTTGGCCCTGGGGATGACGCAGTGAAGCAGCAAAA
  GTGTAACATGTTCTCCCTGGTCTTCTTGGGCCTAGGAGTCCTCTCCTTCTTTACTTTCTTCCTTCAGGGC
  TTCACGTTTGGGAAAGCTGGAGAGATCCTCACCACAAGGCTCCGGTCCATGGCCTTTAAAGCGATGCTAA
  GGCAGGACATGAGCTGGTTTGATGATCATAAAAACAGTACTGGAGCACTTTCTACAAGACTCGCCACAGA
  TGCTGCGCAAGTCCAAGGAGCCACGGGAACCAGGTTGGCTTTAATTGCACAGAACACAGCCAACCTTGGA
  ACCGGTATTATTATATCATTTATTTACGGTTGGCAACTGACACTTCTGCTGTTATCGGTTGTTCCATTCA
  TTGCTGTAGCAGGAATTGTTGAAATGAAAATGTTGGCTGGCAATGCCAAGAGAGATAAAAAGGAAATGGA
  AGCTGCTGGAAAGATTGCAACAGAGGCAATAGAAAATATTCGAACTGTTGTATCCTTGACCCAAGAAAGA
  AAATTTGAGTCAATGTATGTTGAAAAATTGCATGGACCTTACAGGAATTCGGTGCGGAAGGCACACATCT
  ACGGCATCACTTTTAGCATCTCCCAAGCATTCATGTATTTTTCTTATGCTGGCTGTTTTCGATTTGGTTC
  TTACCTAATTGTGAATGGACATATGCGCTTCAAAGATGTCATTCTGGTCTTTTCTGCAATTGTGCTTGGC
  GCGGTGGCTCTAGGACACGCCAGCTCATTTGCTCCGGACTATGCAAAAGCCAAGCTGTCTGCAGCATACT
  TGTTCAGCCTGTTTGAAAGACAACCTCTGATTGACAGCTACAGTGGAGAAGGGCTGTGGCCTGATAAGTT
  TGAAGGAAGCGTGACATTTAATGAAGTCGTGTTCAACTATCCCACCCGGGCCAACGTGCCAGTGCTTCAG
  GGGCTGAGCCTTGAGGTGAAGAAGGGCCAGACGCTGGCCCTGGTGGGCAGCAGTGGCTGCGGGAAGAGCA
  CAGTGGTCCAGCTGCTCGAGCGCTTCTATGACCCCATGGCTGGATCAGTGCTCTTAGATGGTCAAGAAGC
  AAAGAAACTCAATGTCCAGTGGCTCCGAGCTCAACTGGGCATTGTGTCCCAGGAACCCATTCTCTTTGAC
  TGCAGCATCGCAGAGAACATCGCCTATGGAGACAACAGCCGGGTCGTGCCTCATGATGAGATTGTGAGGG
  CAGCCAAGGAGGCCAACATCCACCCCTTCATCGAGACGCTGCCCCAAAAATATAACACAAGAGTAGGAGA
  CAAGGGGACGCAGCTCTCTGGGGGCCAGAAGCAGAGGATTGCCATCGCCCGAGCCCTCATCAGACAGCCT
  CGGGTCCTACTGCTGGATGAAGCCACGTCAGCTCTGGATACTGAGAGTGAAAAGGTTGTCCAGGAAGCAC
  TGGACAAAGCCAGGGAAGGCCGCACCTGCATTGTGATCGCTCACCGCCTGTCCACCATCCAGAACGCGGA
  CTTGATCGTGGTGATTGAGAACGGCAAGGTCAAGGAGCACGGCACCCACCAGCAGCTGCTGGCGCAGAAG
  GGCATCTATTTCTCAATGGTCAACATCCAGGCCGGCACACAGAACTTATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008830
• Insert Size: 3831 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008830.2, NP_032856.2
• RefSeq Size: 4083 bp
• RefSeq ORF: 3831 bp
• Locus ID: 18670
• UniProt ID: P21440
• Cytogenetics: 5 3.43 cM
• Gene Summary: Energy-dependent phospholipid efflux translocator that acts as a positive regulator of biliary lipid secretion. Functions as a floppase that translocates specifically phosphatidylcholine (PC) from the inner to the outer leaflet of the canalicular membrane bilayer into the canaliculi between hepatocytes. Translocation of PC makes the biliary phospholipids available for extraction into the canaliculi lumen by bile salt mixed micelles and therefore protects the biliary tree from the detergent activity of bile salts (PubMed:8106172, PubMed:7912658, PubMed:7592705, PubMed:7814632, PubMed:8725158, PubMed:9366571). Plays a role in the recruitment of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and sphingomyelin (SM) molecules to nonraft membranes and to further enrichment of SM and cholesterol in raft membranes in hepatocytes (By similarity). Required for proper phospholipid bile formation (PubMed:8106172). Indirectly involved in cholesterol efflux activity from hepatocytes into the canalicular lumen in the presence of bile salts in an ATP-dependent manner (PubMed:7814632, PubMed:8725158). May promote biliary phospholipid secretion as canaliculi-containing vesicles from the canalicular plasma membrane (PubMed:9366571). In cooperation with ATP8B1, functions to protect hepatocytes from the deleterious detergent activity of bile salts (PubMed:21820390). Does not confer multidrug resistance (PubMed:1990275).[UniProtKB/Swiss-Prot Function]