Abcb1b (NM_011075) Mouse Untagged Clone

CAT#: MC224124

Abcb1b (untagged) - Mouse ATP-binding cassette, sub-family B (MDR/TAP), member 1B (Abcb1b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Abcb1b
• Synonyms: Abcb1; mdr; Mdr1; Mdr1b; Pgy-1; Pgy1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224124 representing NM_011075
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTTTGAAGAGAACCTTAAGGGAAGAGCAGACAAGAACTTCTCGAAGATGGGCAAAAAGAGTAAAA
  AGGAGAAGAAAGAAAAGAAACCTGCTGTTGGCGTATTTGGGATGTTTCGCTATGCAGATTGGCTGGACAA
  GCTGTGCATGATTCTGGGAACTCTCGCTGCTATTATCCATGGAACATTACTTCCCCTCTTGATGCTGGTG
  TTTGGAAACATGACAGATAGTTTTACAAAAGCAGAAGCCAGTATTCTGCCAAGCATTACTAATCAAAGTG
  GACCCAACAGTACTCTGATCATCAGCAACAGCAGTCTGGAGGAAGAGATGGCCATATACGCCTACTATTA
  CACCGGGATTGGTGCTGGTGTGCTCATAGTTGCCTACATCCAGGTTTCACTTTGGTGCCTGGCAGCTGGA
  AGACAGATACACAAGATTAGGCAGAAGTTTTTCCATGCTATAATGAATCAGGAGATAGGCTGGTTTGATG
  TGCATGATGTTGGGGAGCTCAACACCCGGCTCACAGATGATGTCTCCAAAATTAATGACGGAATTGGTGA
  CAAAATTGGGATGTTTTTTCAGTCCATAACCACATTTTTAGCCGGTTTTATCATAGGATTTATAAGTGGT
  TGGAAGCTAACCCTTGTCATTTTGGCTGTCAGCCCTCTTATTGGATTGTCATCTGCTTTGTGGGCAAAGG
  TATTGACTTCATTTACTAATAAGGAACTCCAGGCTTATGCAAAAGCTGGAGCAGTTGCTGAAGAAGTCTT
  AGCAGCCATCAGAACTGTGATTGCCTTTGGAGGACAACAGAAGGAACTTGAAAGGTACAATAAAAATTTA
  GAAGAAGCTAAAAATGTTGGCATAAAGAAAGCTATCACAGCCAGCATTTCGATAGGCATTGCCTACCTGT
  TGGTCTATGCATCATATGCACTGGCATTCTGGTATGGGACATCCTTGGTCCTCTCAAATGAATATTCTAT
  TGGAGAAGTGCTTACTGTCTTCTTCTCTATTTTGTTGGGGACTTTTAGTATTGGACACTTGGCCCCAAAC
  ATAGAAGCCTTTGCAAACGCACGAGGGGCAGCCTTTGAAATCTTCAAGATAATTGATAACGAGCCAAGCA
  TTGACAGCTTCTCAACAAAGGGCTACAAACCAGACAGTATAATGGGAAACTTAGAGTTTAAAAATGTTCA
  CTTCAACTACCCATCGAGAAGCGAAGTTCAGATCTTGAAGGGCCTCAATCTGAAGGTGAAGAGCGGACAG
  ACGGTGGCCCTGGTTGGCAACAGTGGCTGTGGAAAAAGCACAACTGTCCAGCTGATGCAGAGGCTCTACG
  ACCCCCTGGAGGGCGTGGTCAGTATCGACGGACAAGACATCAGAACCATCAATGTGAGGTATCTGAGGGA
  GATCATTGGTGTGGTGAGTCAGGAACCTGTGCTGTTTGCCACCACGATCGCCGAGAACATTCGCTATGGC
  CGAGAAGATGTCACCATGGATGAGATTGAGAAAGCTGTCAAGGAAGCCAATGCCTATGACTTCATCATGA
  AACTGCCCCACCAATTTGACACCCTGGTTGGTGAGAGAGGGGCGCAGCTGAGTGGGGGACAGAAACAGAG
  AATCGCCATTGCCCGGGCCCTGGTCCGCAATCCCAAGATCCTTTTGTTGGACGAGGCCACCTCAGCCCTG
  GATACAGAAAGTGAAGCTGTGGTGCAGGCCGCACTGGATAAGGCTAGAGAAGGCCGGACCACCATTGTGA
  TAGCTCATCGCTTGTCTACAGTTCGTAATGCTGACGTCATTGCTGGTTTTGATGGTGGTGTCATTGTGGA
  GCAAGGAAATCATGATGAGCTCATGAGAGAAAAGGGCATTTACTTCAAACTTGTCATGACACAGACTAGA
  GGAAATGAAATTGAACCAGGAAATAATGCTTATGGATCCCAGAGTGACACTGATGCTTCTGAACTGACTT
  CAGAAGAATCCAAATCACCTTTAATAAGGAGATCAATTTACAGAAGTGTCCACAGAAAGCAAGACCAAGA
  GAGAAGACTTAGTATGAAAGAGGCTGTGGATGAAGATGTGCCTCTGGTTTCCTTTTGGCGGATCCTAAAT
  CTAAATCTAAGTGAATGGCCTTATTTACTTGTTGGCGTACTTTGCGCTGTTATAAATGGGTGCATACAAC
  CAGTGTTTGCCATAGTATTTTCAAGGATTGTAGGGGTTTTTTCAAGAGATGATGACCATGAAACTAAACG
  ACAGAATTGTAATTTGTTTTCCCTGTTCTTTCTGGTTATGGGGCTGATTTCTTTTGTTACATATTTCTTT
  CAGGGCTTCACATTTGGCAAAGCCGGAGAGATCCTCACCAAGCGAGTCCGATACATGGTTTTCAAATCCA
  TGCTGAGACAGGATATAAGCTGGTTCGATGACCATAAGAACAGCACTGGCTCACTGACCACCAGGCTCGC
  CAGTGATGCTTCTAGTGTTAAAGGGGCGATGGGCGCCAGGCTTGCTGTAGTTACCCAGAATGTAGCAAAC
  CTCGGGACAGGAGTCATCCTCTCCTTAGTCTATGGCTGGCAGCTGACACTTCTACTTGTAGTAATTATAC
  CGCTCATTGTATTGGGCGGAATTATTGAAATGAAGCTGTTGTCTGGCCAAGCCTTGAAGGACAAGAAACA
  GCTTGAGATCTCTGGGAAGATTGCTACAGAAGCAATTGAAAACTTCCGCACTATTGTCTCTTTGACTCGG
  GAGCAGAAGTTTGAAACCATGTATGCCCAGAGCTTGCAGGTACCATACAGAAATGCGATGAAGAAAGCAC
  ACGTGTTTGGGATCACGTTCTCCTTCACCCAGGCCATGATGTATTTTTCTTATGCTGCTTGTTTCCGGTT
  CGGTGCCTACTTGGTGGCACAACAACTCATGACTTTTGAAAATGTTATGTTGGTATTTTCTGCTGTTGTC
  TTTGGTGCCATGGCAGCTGGGAATACTAGTTCATTTGCTCCTGACTATGCGAAAGCCAAAGTATCAGCAT
  CTCATATCATCAGGATCATTGAGAAAACCCCTGAGATTGACAGCTACAGCACAGAGGGCTTGAAGCCTAC
  TCTGTTAGAAGGAAATGTAAAATTTAATGGAGTCCAGTTTAACTATCCCACCCGACCCAACATCCCAGTG
  CTTCAGGGGCTGAGCCTCGAGGTGAAGAAGGGCCAGACGTTGGCCCTGGTGGGCAGCAGTGGCTGTGGGA
  AGAGCACAGTGGTCCAGCTGCTCGAGCGCTTCTACGACCCCATGGCTGGATCAGTGTTTCTAGATGGCAA
  AGAAATAAAGCAACTGAATGTCCAGTGGCTCCGAGCTCACCTTGGCATTGTGTCCCAGGAGCCCATTCTC
  TTTGACTGCAGCATTGCAGAGAACATCGCCTATGGAGACAACAGCCGGGCCGTGTCTCATGAGGAGATTG
  TGAGGGCAGCCAAGGAGGCCAACATCCACCAGTTCATCGACTCACTGCCTGATAAATACAACACCAGAGT
  AGGAGACAAAGGCACTCAGCTGTCGGGTGGGCAGAAGCAGCGCATCGCCATCGCACGTGCCCTCGTCAGA
  CAGCCTCACATTTTACTTCTGGACGAAGCAACATCAGCTCTGGATACAGAAAGTGAAAAGGTTGTCCAGG
  AAGCGCTGGACAAAGCCAGGGAAGGCCGCACCTGCATTGTGATCGCTCACCGCCTGTCCACCATCCAGAA
  CGCGGACTTGATCGTGGTGATTGAGAACGGCAAAGTCAAGGAGCACGGCACCCACCAGCAGCTGCTGGCG
  CAGAAGGGCATCTACTTCTCAATGGTCCAGGCTGGAGCAAAGCGCTCATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011075
• Insert Size: 3831 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011075.2, NP_035205.1
• RefSeq Size: 4344 bp
• RefSeq ORF: 3831 bp
• Locus ID: 18669
• UniProt ID: P06795
• Cytogenetics: 5 3.43 cM
• Gene Summary: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. This gene encodes a membrane glycoprotein which confers a multidrug-resistance phenotype. The protein encoded by the human gene is an ATP-dependent drug efflux pump for xenobiotic compounds which is responsible for decreased drug accumulation in multidrug-resistant cells and mediates the development of resistance to anticancer drugs. [provided by RefSeq, Jul 2008]