Rpgrip1l (NM_173431) Mouse Untagged Clone

CAT#: MC224118

Rpgrip1l (untagged) - Mouse Rpgrip1-like (Rpgrip1l), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rpgrip1l
• Synonyms: 1700047E16Rik; 4931437C01; Ftm; Nphp8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224118 representing NM_173431
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGGCCCAAGTGACGAGACAGCAGGAGACCTGCCGGTGAAAGATACAGGTCTAAACCTCTTTGGAG
  TTGGAGGGTTACAAGAAACCTCAACTGCACGCACAGTGAAGACTCGCCAGGCAGTGTCACGTGTCAGTCG
  TGAAGAACTGGAAGACAGATTTTTGCGTTTGCATGATGAGAACATTTTACTTAAACAGCATGCCCGCAAG
  CAAGAGGATAAAATTAAAAGAATGGCCACCAAGTTAATACGGCTAGTTAATGACAAGAAAAGATATGAGC
  GGGTTGGTGGCGGCCCCAAGCGGCTGGGACGAGATGTGGAAATGGAAGAAATGATTGAGCAGCTGCAAGA
  AAAAGTTCATGAGCTTGAAAGACAAAATGAAGTTCTCAAAAACAGGCTGATATCAGCCAAACAGCAGCTC
  CAAGTCCAGGGACACAGGCAGACGTCGTACAGTCGAGTGCAGGCTCGAGTTAACACCGGCCGCCGGAGAG
  CAAGTGCCAGTGCAGGCTCGCAGGAATGCCCTGGGAAAGGTCTAAGATTTCAAAATGTGGATGAAGCAGA
  AACTGTACAACCCACGCTCACAAAATACAGCAATAGTTTACTTGAAGAAGCCAGAGGAGAAATAAGAAAT
  TTGGAAAATGTTATTCAGTCACAGAGAGGCCAGATAGAAGAGTTAGAGCACTTGGCAGAGATCCTGAAAA
  CTCAGTTGAAGAGAAAAGAAAATGAAATCGAGCTATCTCTTCTTCAGCTTCGAGAACAGCAGGCTACAGA
  TCAGAGGTCAAATATTCGGGACAATGTAGAAACGATTAAGCTTCATAAACAACTAGTGGAGAAAAGCAAT
  GCTCTTTCAGTCATAGAAGGAAAATTTATTCAGCTTCAAGAGAAGCAAAGAACACTCAGGATCAGCCATG
  ATGCCTTGATGGCCAATGGAGATGAGCTGAACAAGCAGCTTAAGGAGCAGCGTCTGAAGTGCTGCAGCCT
  TGAGAAGCAGCTGCACTCGGTGAGGTTTTCAGAAAGGAGAGTAGAGGAGCTGCAGGATAGAATTAATGAT
  TTAGAAAAGGAGCGGGAACTATTAAAGGAAAACTATGATAAACTTTATAACAGCGCCTTCAGTGCTGCCC
  ATGAAGAGCAGTGGAAGTTAAAGGAGCAGCAGATGAAAGTGCAGATTGCTCAGCTCGAAACTGCCTTAAA
  ATCTGATCTTACAGACAAAACCGAAGTCCTTGACAAACTAAAGACTGAAAGAGACCAAAATGAGAAACTG
  GTTCAAGAGAATAGAGATCTACAGCTGCAGTGTCTGCAGCAAAAGCAGCGGCTGCATGAGCTTCAGAGCC
  GCCTGAAGTTCTTCAACCAGGAGAGTGACATCAATGCTGATGACCTCAGTGAAGCACTCCTGCTTATAAA
  GGCTCAAAAAGAACAAAAAAATGGAGATCTTTCATTTTTAGAAAAAGTGGACAGCAAAATCAATAAAGAT
  CTAGACCGATCCATGAAAGAGCTTCAAGCAACTCATGCAGAAACAGTACAAGAACTTGAAAAGACAAGAA
  ATATGTTAATTATGCAACATAAAATAAATAAAGACTATCAGATGGAAGTTGAAACAGTGACTCAGAAAAT
  GGAAAATTTGCAACAAGATTATGAACTCAAAGTGGAACAATATGTTCATCTTCTCGATATCAGGGCTGCA
  CGCATCCAAAAGCTTGAAGCCCAGTTAAAGGATATTGCCTATGGCACTAAGCAGTACAAATTTAAACCAG
  AAATCATGCCAGATGACTCAGTTGATGAATTTGATGAAACCATCCACCTAGAACGAGGAGAAAATCTCTT
  TGAAATCCACATCAACAAAGTCACCTTTTCTTCTGAAGTCCTTCGGGCATCTGGTGATAAAGAACTTGTC
  ACTTTCTGTACCTACGCTTTCTATGACTTTGAACTGCAGACTACTCCCATAGTGCGAGGCCTTTACCCGG
  AGTATAACTTCACTTCTCAGTATCTGGTCCACGTCAATGACTTATTTTTGCAGTACATTCAGAAGAACAC
  TGTCACGCTGGAGCTCCACCAAGCCCACAGCACAGATTACGAAACAATTGCAGCGTGTCAGTTGAGATTC
  CATGAGATTCTAGAAAAAAGTGGTAGAATTTTTTGCACAACAAGCTTAGTTGGAACTAAAGGAGACATCC
  CAAATTTTGGCACAGTAGAATACTGGTTCCGATTAAGAGTTCCCATGGATCAAGCTATTCGACTTTATCG
  TGAGCGGGCAAAGGCATTAGGATACATAACATCAAATTTTAAGAAGCCAGAGAAAATGCAATTGTCGAGT
  CAGCAAGCAGCCACAACTGCTCAGATCAGCCCAGCAGAGTCCACAGACGGCAACTTGAATGAACTTCATG
  TTACAGTAAAATGCTGCACCGGCCTCCAGTCCCGAGCGAGCTACCTGCAGCCACACGCATATGTGGTATA
  CAAGTTTTTTGATTTTCCAGACCATGATACCGCCATCGTTCCCAGTAGCAATGACCCACAGTTTGATGAC
  CACATGTGTTTCCCAGTGCCGATGAATATGGATTTGGATCGCTACCTTAAGTCAGAGTCTCTGAGTTTCT
  ATGTTTTTGATGATAGTGATACCCAGGAGAATATTTACATGGGAAAAGTCAATGTGCCTCTGATTTCCCT
  GGCACATGACAAGTGTATCTCAGGAATATTTGAGTTGATGGATAAGGAAAAGCATGCTGCAGGAACTATC
  CAAGTTATATTGAAATGGAAATTCACCTACCTTCCACCAAGTGGATCAATAACTACTGAAGACCTTGGAA
  AGTTCGTTTGCAGAGAAGAACCAGAGGCTGTTCAGAGACTACCTCCAAAGTCCTCAGATGTTACATCAGT
  TGTAGCACCAAAACCTAAACCAAGACAACGTTTAACATTTGTGGACAAAAAGGTCTCTTTTGCAGATACC
  ATATCACACCCGAGTCCCGAGACTTCTCCTCCCCCTAAAGATATAAAAGACAGTTCTCCAGAAGTGGGAC
  CAAAACCAGAAAATGGTCTGTCAGCTGTTGCGTATCCTTCCAAGGAAAGCGGTGTGGCTAAGGTAGAAGA
  AAATGTGGGGGAAATGCAACAAGGGAAAGAAGACGACATCTCTTTCTTATCTGAGGGCCAGCTTGCCTCA
  GGAAGTGTGGCTTCCTCTGAAGATGAAACAGAAATAACTGAAGAATTGGAACCAGAAGATGAAGACAGAT
  CAGCTTCAGACAGTGATGACTGTATCATTCCAAGTTCTGTCTCCACAAATACCAAACAGCCATCAGAAGA
  AATTCGGATTGAGATCATAGCTCTGAACCTTAATGATTCTCAAATAACCAGGGAGGATACTATTCAGCGA
  CTCTTCATTGAGTGCCGGTTCTACAGTCTTCCCGCTGAGGAGACTCCTATGTCCCTGCCCAAACCCCAGA
  GTGGACAGTGGGTCTACTATAACTATTCCAATGTGATCTACCTGGATAAAGAAAACAACCCAGCGGTGAG
  AGACATCTTAAAAGCCATACTGCAAAGGCGAGAACTGCCTCACAGAAGTGTGCGCTTCACCGTGGTCAGT
  GATCCTCCCGAGGACGAGCAGGACCTAGAATGTGAGGACATTGGGGTTGCCCATGTTGACCTTGCTGACC
  TGTTTCAGAAAGGAAGAGACATCATAGAGCAAGATATTGATGTTCTCGATGCCCGAACAGACGGTGGCAC
  CATCGGCAAACTGAAGGTGACTGTGGAAGCACTTCATGCCCTACGGTCTGTCTACGAGCAAAATAGAAAG
  GACTTGGAGGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_173431
• Insert Size: 3795 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173431.2, NP_775607.2
• RefSeq Size: 6969 bp
• RefSeq ORF: 3795 bp
• Locus ID: 244585
• UniProt ID: Q8CG73
• Cytogenetics: 8 44.29 cM
• Gene Summary: Negatively regulates signaling through the G-protein coupled thromboxane A2 receptor (TBXA2R) (By similarity). May be involved in mechanisms like programmed cell death, craniofacial development, patterning of the limbs, and formation of the left-right axis. Involved in the organization of apical junctions; the function is proposed to implicate a NPHP1-4-8 module. Does not seem to be strictly required for ciliogenesis (By similarity). Involved in establishment of planar cell polarity such as in cochlear sensory epithelium and is proposed to implicate stabilization of disheveled proteins (PubMed:22927466). Involved in regulation of proteasomal activity at the primary cilium probably implicating association with PSDM2 (PubMed:26150391).[UniProtKB/Swiss-Prot Function]