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Nrcam (NM_176930) Mouse Untagged Clone

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SKU
MC224099
Nrcam (untagged) - Mouse neuron-glia-CAM-related cell adhesion molecule (Nrcam), transcript variant 1, (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $1,203.00
5 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Nrcam
Synonyms Bravo; C030017F07Rik; C130076O07Rik; mKIAA0343
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC224099 representing NM_176930
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGCTTAAAATAATGCCGAAGAAGAAGCACTTATCTGCAGGCGGAGTGCCCCTGATTCTCTTCCTGT
GCCAGATGATCAGCGCGCTGGATGTTCCTCTCGATCTGGTACAACCTCCAACCATCACTCAACAGTCACC
AAAAGACTACATCATTGACCCTCGGGAGAATATTGTAATCCAGTGTGAGGCCAAAGGGAAACCTCCCCCA
AGCTTTTCCTGGACTCGTAATGGAACTCACTTTGACATAGACAAGGACCCTCTGGTCACCATGAAGCCTG
GCTCAGGAACCCTTGTCATCAACATCATGAGTGAGGGGAAGGCAGAGACCTATGAAGGAGTCTACCAGTG
CACTGCAAGGAACGAGCGTGGAGCTGCCGTCTCCAATAACATTGTTGTCCGCCCATCTAGGTCACCCTTG
TGGACCAAGGAAAGACTTGAACCTATAGTACTCCAGAATGGGCAGTCATTAGTACTTCCATGTAGGCCTC
CGATTGGATTACCTCCGGCCATAATATTTTGGATGGATAATTCCTTTCAGAGACTTCCACAGAGTGAGCG
GGTTTCCCAAGGCCTAAATGGAGACCTTTACTTCTCCAATGTCCTCCCAGAGGACACCCGTGAGGACTAT
ATCTGCTATGCCAGATTTAATCACACTCAAACCATCCAGCAGAAGCAGCCTATTTCTTTGAAGGTGATTT
CAGTGGATGAATTGAATGACACTATAGCTGCTAATTTGAGTGACACTGAGTTTTATGGTGCTAAATCTAG
TAAAGAGAGGCCCCCAACGTTTCTAACTCCAGAGGGCAATGAAAGCCACAAAGAGGAATTAAGAGGAAAC
GTGCTTTCGCTGGAGTGCATTGCGGAAGGCCTACCTACTCCAATTATTTACTGGATCAAAGAAGACGGAA
TGCTTCCCGCCAACCGGACATTTTATCGGAACTTTAAGAAAACCCTGCAGATCACTCATGTTTCCGAAGC
CGACTCTGGAAATTATCAGTGCATAGCAAAAAACGCATTAGGAGCCGTCCATCACACCATTTCTGTCACT
GTTAAAGCGGCTCCCTACTGGATCGTGGCACCTCAAAACCTCGTGCTTTCCCCGGGAGAGAATGGGACCC
TCATCTGCAGAGCTAATGGCAACCCGAAACCCAGAATTAGCTGGTTAACAAATGGAGTCCCAATAGAAAT
TGCTCTCGATGACCCCAGCAGAAAAATAGATGGCGATACCATTATATTTTCAAATGTTCAAGAAAGCTCA
AGTGCGGTTTATCAGTGCAATGCCTCTAACAAATATGGATATTTACTAGCAAATGCATTTGTAAATGTTC
TCGCTGAACCACCTCGGATTCTCACATCAGCAAACACACTGTACCAGGTCATTGCAAACAGGCCTGCTTT
GCTAGATTGTGCCTTCTTTGGATCTCCTATGCCTACCATTGAGTGGTTTAAAGGCACTAAAGGAAGCGCT
CTTCATGAAGACATTTATGTTTTGCATGATAATGGAACATTAGAAATTCCCGTGGCCCAAAAGGATAGTA
CAGGGACGTATACTTGTGTCGCACGGAATAAACTAGGGATGGCAAAGAATGAAGTTCACTTGGAAATCAA
AGATCCAACCAGGATCATTAAACAACCTGAGTATGCAGTCGTCCAGAGGGGGAGCAAGGTGTCCTTTGAA
TGCAGAGTGAAACATGACCACACCTTAATCCCCACCATTATGTGGCTGAAGGACAATGGAGAGCTGCCCA
ATGATGAAAGGTTCTCCACTGACAAGGATCATCTGGTGGTATCTGATGTAAAGGATGACGATGGCGGAAC
CTACACGTGTACGGCCAACACAACGCTGGACAGTGCTTCGGCCAGCGCTGTGCTCAGGGTTGTCGCTCCT
ACTCCAACTCCAGCCCCCATTTACGATGTCCCGAATCCTCCCTTTGATTTAGAATTGACCAATCAACTTG
ACAAAAGTGTTCAGCTGACATGGACCCCAGGCGACGACAACAATAGCCCCATTACAAAATTCATCATTGA
GTATGAAGATGCAATGCATGATGCAGGGCTGTGGCGCCACCAGGCTGAAGTTTCTGGAACACAGACCACA
GCCCAACTGAAGCTGTCTCCCTATGTGAACTACTCCTTCCGTGTCATGGCAGAGAACAGCATTGGGAGAA
GTATGCCGAGCGAGGCATCCGAGCAGTATCTTACAAAAGCCGCAGAACCAGATCAGAATCCCATGGCTGT
GGAAGGACTAGGGACAGAGCCGGACAACTTGGTGATTACATGGAAGCCCCTGAATGGTTTTCAATCGAAT
GGGCCTGGCCTCCAGTACAAAGTGAGCTGGCGCCAGAAAGATGGTGACGATGAGTGGACGTCTGTGGTTG
TGGCCAATGTATCCAAATACATTGTTTCTGGCACACCAACCTTTGTCCCATACCTGATAAAAGTTCAAGC
TCTGAATGATGTGGGGTTTGCACCAGAGCCAGCTGCAGTCATGGGGCATTCTGGAGAAGACCTTCCAATG
GTGGCTCCTGGAAATGTTCGCGTCAGCGTGGTGAACAGTACGCTGGCAGAGGTGCACTGGGACCCAGTTC
CTCCCAAGAGTGTCCGAGGACACTTACAAGGCTACCGGATTTACTACTGGAAGACCCAGAGCTCCTCTAA
AAGAAACAGGCGCCACATTGAGAAGAAGATCCTCACCTTCCAGGGCACCAAGACTCACGGCATGCTGCCA
GGGCTGCAGCCATACAGTCACTATGCCCTCAACGTCCGAGTGGTCAACGGGAAAGGGGAGGGCCCAGCCA
GCACGGACAGAGGCTTCCATACACCGGAGGGAGTCCCTAGTGCTCCCTCATCTTTGAAAATTGTTAATCC
CACACTGGACTCCCTCACTTTGGAATGGGACCCCCCAAGCCACCCAAATGGCATCCTGACTGAGTACATC
TTACAATATCAGCCAATTAACAGCACACATGAGCTAGGCCCTCTGGTAGATTTAAAAATTCCTGCCAACA
AGACCCGCTGGACTTTGAAAAATTTAAATTTCAGCACTCGGTACAAGTTCTATTTCTATGCACAGACATC
AGTGGGGCCAGGCAGTCAGATCACAGAGGAAGCGATAACGACTGTGGACGAAGCTGGTATTCCTCCACCT
GATGTAGGTGCAGGCAAAGGCAAAGAAGAATGGAGGAAAGAAATTGTAAATGGTTCTCGAAGCTTCTTTG
GGTTAAAGGGTCTAATGCCAGGAACAGCATACAAAGTTCGAGTTGGTGCTGAGGGGGACTCTGGTTTTGT
GAGTTCAGAGGATGTGTTTGAGACAGGACCAGCAATGGCAAGCCGGCAAGTGGATATCGCGACCCAAGGC
TGGTTCATAGGTCTAATGTGCGCTGTTGCTCTCCTCATCTTAATTTTGCTGATTGTTTGCTTCATCAGAA
GAAACAAAGGTGGTAAATACCCAGTTAAAGAAAAGGAGGATGCTCATGCAGACCCTGAAATCCAGCCCAT
GAAGGAAGATGATGGGACGTTTGGAGAATACAGTGATGCAGAAGATCACAAGCCTTTGAAAAAAGGAAGT
CGAACACCTTCAGACAGGACTGTGAAAAAAGAAGATAGCGATGATAGTCTGGTTGACTATGGAGAGGGGG
TGAACGGCCAATTCAACGAGGATGGCTCCTTTATTGGCCAATACAGTGGTAAGAAAGAGAAAGAGCCAGC
AGAGGGAAATGAAAGCTCAGAGGCCCCTTCTCCTGTCAACGCAATGAACTCCTTTGTTTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_176930
Insert Size 3771 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_176930.4, NP_795904.3
RefSeq Size 7572 bp
RefSeq ORF 3771 bp
Locus ID 319504
UniProt ID Q810U4
Cytogenetics 12 20.71 cM
Summary Cell adhesion protein that is required for normal responses to cell-cell contacts in brain and in the peripheral nervous system. Plays a role in neurite outgrowth in response to contactin binding (PubMed:11564762). Plays a role in mediating cell-cell contacts between Schwann cells and axons (PubMed:20188654). Plays a role in the formation and maintenance of the nodes of Ranvier on myelinated axons. Nodes of Ranvier contain clustered sodium channels that are crucial for the saltatory propagation of action potentials along myelinated axons. During development, nodes of Ranvier are formed by the fusion of two heminodes. Required for normal clustering of sodium channels at heminodes; not required for the formation of mature nodes with normal sodium channel clusters (PubMed:14602817, PubMed:20188654). Required, together with GLDN, for maintaining NFASC and sodium channel clusters at mature nodes of Ranvier (PubMed:24719088).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_176930" in other vectors (4)
SKU Description Size Price
MG219511 Nrcam (tGFP-tagged) - Mouse neuron-glia-CAM-related cell adhesion molecule (Nrcam) transcript variant 1, (10ug) 10 ug
$1,293.00
MR219511 Nrcam (Myc-DDK-tagged) - Mouse neuron-glia-CAM-related cell adhesion molecule (Nrcam), transcript variant 1 10 ug
$589.00 $1,093.00
MR219511L3 Lenti ORF clone of Nrcam (Myc-DDK-tagged) - Mouse neuron-glia-CAM-related cell adhesion molecule (Nrcam), transcript variant 1 10 ug
$1,393.00
MR219511L4 Lenti ORF clone of Nrcam (mGFP-tagged) - Mouse neuron-glia-CAM-related cell adhesion molecule (Nrcam), transcript variant 1 10 ug
$1,393.00

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