Mtr (NM_001081128) Mouse Untagged Clone

CAT#: MC224074

Mtr (untagged) - Mouse 5-methyltetrahydrofolate-homocysteine methyltransferase (Mtr), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mtr
• Synonyms: AI894170; D830038K18Rik; MS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224074 representing NM_001081128
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAAAACCCTGCAGGATGAGATTGAAGCCATTCTTCGGAAGAGGATCATGGTGCTAGACGGTGGGA
  TGGGGACCATGATCCAGCGGTACAAACTAAGTGAAGAACATTTCCAAGGTCAAGAGTTTAAAGATCACAG
  CAGGCCACTGAAAGGCAACAATGACATCTTAAGTATAACTCAGCCTGATATTATTTACCAGATTCATAAG
  GAATACTTGCTGGCTGGAGCAGATATCATCGAAACAAACACTTTCAGCAGTACTAGTATCGCCCAGGCTG
  ACTATGGCCTTGAACACTTGGCCTACCGGATGAACAAGTGCTCTGCAGATGTGGCCAGAAAAGCCGCTGA
  GGAGATAACTCTGCAGACAGGAGTCAAGAGGTTTGTGGCTGGAGCTCTGGGTCCGACTAATAAGACACTT
  TCTGTCTCCCCATCTGTGGAAAGGCCAGATTATAGGAACATCACATTTGATGAGCTTGTTGACGCATACC
  AGGAGCAGGCCAAGGGACTGCTGGATGGCAGGGTCGACATCTTACTCATTGAAACAATTTTTGATACAGC
  TAATGCCAAAGCAGCCTTGTTTGCGATCCAGAACCTCTTTGAAGAGAATTATGCTCCTCCTCGGCCTATC
  TTTATTTCTGGGACCATTGTTGATAAAAGTGGAAGGACTCTTTCTGGGCAGACGGGAGAGGCTTTTGTCA
  CCAGCGTGTCTCATTCGGACCCCCTGTGCATTGGATTAAATTGTTCTCTGGGTGCAGCTGAAATGAGGCC
  TTTCATTGAAACAATTGGAAAATGTACAACAGCCTATGTCCTCTGTTACCCAAATGCAGGTCTTCCCAAC
  ACTTTTGGCGACTATGATGAAACACCATCCACGATGGCCACGCACCTGAAGGACTTTGCTGTGGATGGCT
  TGGTGAATATAGTTGGTGGATGCTGTGGTTCAACACCTGATCACATCAGGGAAATTGCGGAAGCTGTGAA
  AAAGTGTAAGCCAAGAGTCCCCCCTGCTAGTGTTTTTGAAGGACATATGTTACTGTCTGGTCTAGAGCCC
  TTCAGGATTGGACCATACACCAACTTTGTTAACATTGGAGAGCGCTGTAATGTGGCAGGATCCAGGAAAT
  TTGCTAAACTCATCATGGCAGGAAACTATGAAGAAGCCCTGAGCATTGCCAAAGCACAGGTGGAAATGGG
  AGCTCAGGTGCTCGATATCAACATGGACGACGGCATGCTGGATGGTCCCAGTGCAATGACCAGATTTTGC
  AACTCCATTGCTTCTGAGCCTGACATTGCCAAGGTGCCCCTGTGCATTGACTCTTCTAACTTTGCCGTGA
  TTGAAGCTGGGTTGAAGTGCTGCCAAGGGAAGTGCATAGTCAACAGCATCAGCCTCAAGGAGGGGGAGGG
  GGACTTCCTGGAGAAGGCCCGGAAGATTAAGAAGTTTGGAGCTGCTGTGGTGGTTATGGCTTTTGATGAG
  GAAGGACAAGCAACAGAAACAGATGTTAAAGTCAATGTGTGCACCAGAGCTTACCATCTACTTGTGGACA
  AAGTGGGCTTCAATCCAAATGACATCATCTTTGACCCCAACATCCTTACCATTGGTACTGGAATGGAGGA
  ACATAACTTGTATGCTATCAATTTTATCCATGCAACAAGAGTCATTAAGGAAACCTTACCAGGAGTCAGA
  ATAAGTGGAGGTCTTTCCAACTTATCCTTCTCCTTCCGAGGGATGGAAGCCATTCGAGAAGCAATGCATG
  GTGTGTTCCTTTATCACGCGATCAAGTTTGGTATGGACATGGGGATAGTCAATGCCGGCAACCTCCCTGT
  GTATGATGCTATCCACAAGGACCTTCTTCAGCTCTGTGAAGACCTCATCTGGAACAAAGACTCTGAGGCT
  ACTGAGAAACTCTTGCGATATGCCCAGACTCATGGCACAGGAGGGAAGAAAGTCATCCAGACAGATGAGT
  GGAGGAATGGCTCCATTGAAGAGCGCCTGGAGTATGCTCTTGTGAAGGGCATTGAAAAGCACATTGTTGA
  AGATACTGAAGAAGCCAGGTTAAACGGGGAAAAATACCCTCGGCCTCTGAATATAATTGAAGGGCCCCTA
  ATGAATGGCATGAAAGTGGTTGGTGATCTTTTTGGAGCTGGAAAGATGTTTCTACCTCAGGTTATAAAGT
  CAGCCCGTGTCATGAAGAAGGCTGTTGGCCACCTTATCCCTTTCATGGAAAAAGAAAGAGAAGAAGCCAG
  ATTGATTAATGGTTCGGTTGAAGAAGAGGACCCTTACCAAGGCACCATTGTGCTGGCCACTGTTAAAGGG
  GACGTGCATGACATCGGCAAGAACATAGTTGGCGTGGTGCTGGCCTGTAACAATTTCAGAGTTATTGATT
  TAGGAGTCATGACTCCCTGTGATAAGATACTTCAAGCTGCTCTGGACCACAAAGCAGATATAATTGGCTT
  GTCAGGACTCATCACTCCATCCTTGGATGAGATGATTTTTGTTGCCAAGGAAATGGAGAGGTTGGCTATA
  AAGATTCCATTGTTGATAGGAGGAGCAACCACTTCAAGAACCCACACAGCAGTTAAAATTGCACCACGGT
  ACAGCGCGCCTGTGATCCACGTCCTAGATGCATCCAAGAGTGTGGTGGTGTGTTCTCAGCTGTTAGATGA
  AAATCTGAGAGATGACTACTTTGAAGAAATACTGGAAGAGTATGAAGATATTAGACAGGACCACTATGAG
  TCTCTCAAAGAGAGAAAATACGTACCCCTAAGTCAAGCCAGAAAACACGGTTTCCACATTGATTGGCTCT
  CTGAACCTCATCCAGTGAAGCCCACGTTTATTGGGACCCAGGTCTTTGAGGACTACAACCTGCAAAAGCT
  GGTGGACTACATTGACTGGAAGCCTTTCTTTGACGTCTGGCAGCTCCGGGGCAAGTACCCGAACCGAGGA
  TTTCCCAAGATATTTAATGACAAAGCAGTAGGTGAAGAAGCCAGAAAGGTATACAATGATGCTCAGAATA
  TGCTGAACATACTGATTAGTCAAAAGAAACTGCAGGCCAGGGGTGTGGTTGGATTCTGGCCAGCACAGAG
  TGTCCAGGATGACATCCACCTGTATGCAGAGGGGGTGGTGCCCCAGGCCGCTGAGCCCATAGCCACCTTC
  TATGGACTGAGGCAGCAGGCTGAGAAGGACTCTTCTAGTACAGACCCCTACCACTGCCTCTCAGACTTCA
  TTGCTCCTTTGCATTCTGGTGTCTGTGACTACTTGGGTCTGTTTGCTGTTGCCTGCTTTGGGGTTGAAGA
  GCTGAGTAAGACCTATGAGGATGATGGCGATGACTACAGCAGCATCATGGTGAAGGCGCTGGGCGACAGG
  CTGGCAGAGGCCTTTGCAGAGGAGCTCCATGAGAGAGTTCGCCGAGAACTGTGGGCCTACTCTAGGAGTG
  AGCAGTTGGGCGTCCCAGACTTGCGCAGACTCCGGTATGAGGGCATCCGGCCAGCTCCTGGTTACCCCAG
  CCAGCCTGACCATACTGAGAAGCTTACCATGTGGAGACTGGCCAGCATCGAGCAGGCCACAGGCATCAGG
  CTGACAGAATCCTTGGCAATGGCACCCGCTTCAGCAGTGTCAGGGCTGTACTTCTCCAACGTAAAGGCCA
  AATATTTTGCTGTGGGGAAAATTTCCAAAGATCAGACTGAGGATTATGCTTTGAGGAAGAACATGCCAGT
  GGCCGAGGTGGAGAAATGGCTTGGTCCCATTCTGGGCTATGACACAGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081128
• Insert Size: 3762 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081128.3, NP_001074597.1
• RefSeq Size: 4487 bp
• RefSeq ORF: 3762 bp
• Locus ID: 238505
• UniProt ID: A6H5Y3
• Cytogenetics: 13 4.52 cM
• Gene Summary: Catalyzes the transfer of a methyl group from methyl-cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate (By similarity).[UniProtKB/Swiss-Prot Function]