Ehmt1 (NM_001109687) Mouse Untagged Clone

CAT#: MC224068

Ehmt1 (untagged) - Mouse euchromatic histone methyltransferase 1 (Ehmt1), transcript variant 4, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ehmt1
• Synonyms: 9230102N17Rik; D330003E03; Eu-HMTase1; GLP; GLP1; KMT1D; mKIAA1876
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224068 representing NM_001109687
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCCGCTGATGCTGAGCAGGCAGTTCTGGCCAAGCAAGAGACCAAGCAGGATTGCTGCATGAAAA
  CTGAGCTGCTAAGGGAAGATACACCTATGGCTGCTGATGAAGGTTCCACAGAGAAACAAGAAGGAGAGAC
  TCCCATGGCTGCAGATGGAGAAACAAATGGGTCTTGTGAAAAGAGTGGGGATCCCAGCCATCTAAATGCA
  CCCAAACACACTCAGGAGAACACAAGAGCTAGCCCACAGGAAGGCACCAACAGAGTGTCTCGGGTGGCAG
  AAAATGGGGTTTCAGAAAGAGACACAGAAGTGGGGAAGCAAAACCATGTCACAGCTGACGACTTCATGCA
  GACATCTGTCATTGGCAGCAATGGATATTTCTTAAATAAACCAGCCCTGCAGGGGCAGCCGTTGAGGACT
  CCCAACATTCTAACCTCCTCGCTTCCTGGTCATGCTGCAAAAACTCTTCCTGGAGGAGCCAGTAAATGCA
  GGACTCTGAGTGCACTTCCTCAGACACCAACCACAGCACCCACTGTGCCTGGGGAAGGGAGTGCAGACAC
  AGAGGACAGAAAGCCTACAGCCTCGGGCACTGATGTCAGGGTTCACAGGGCACGCAAGACCATGCCGAAG
  TCCATCTTGGGCCTGCATGCAGCCAGCAAAGACCATAGAGAAGTTCAAGACCATAAGGAACCAAAAGAGG
  ATATCAACAGAAACATTTCTGAATGTGGACGACAGCAGCTTTTACCAACCTTCCCAGCCCTCCACCAGTC
  GCTACCTCAGAATCAGTGCTACATGGCCACCACAAAGTCCCAGACAGCTTGCTTGCCTTTTGTTTTAGCA
  GCTGCAGTATCTCGGAAGAAAAAACGAAGAATGGGAACCTATAGTTTAGTTCCCAAGAAAAAGACCAAAG
  TATTAAAACAGAGGACGGTGATTGAGATGTTTAAAAGCATAACCCATTCCACTGTGGGCGCCAAGGGCGA
  GAAAGCCTTAGATGATAGTGCCCTGCATGTAAATGGCGAGAGCTTGGAGATGGACTCAGAAGATGAAGAC
  TCCGATGAGCTGGAGGATGACGAGGACCATGGAGCTGAGCAAGCGGCTGCATTTCCCACCGAGGATAGTA
  GGACTTCTAAAGAGAGCATGTCTGAGACTGACCGGGCTGCAAAGATGGATGGAGATTCAGAGGAGGAACA
  GGAGTCTCCCGACACAGGGGAGGATGAAGATGGTGGAGACGAGTCTGACCTGAGTTCTGAATCCAGTATC
  AAGAAGAAATTTCTCAAGAGGAGAGGGAAGACTGACAGCCCCTGGATCAAACCTGCTCGGAAAAGGAGGC
  GGAGAAGTAGAAAGAAGCCGAGCAGCATGCTTGGTTCCGAGGCTTGTAAGTCATCTCCAGGAAGCATGGA
  GCAGGCAGCTCTGGGAGACAGCGCTGGCTATATGGAAGTTTCCCTGGATTCCCTGGATCTCCGTGTCAGA
  GGAATTCTGTCTTCCCAGACAGAAAATGAAGGGCTGGCCAGTGGTCCGGATGTGCTGGGGACGGATGGCC
  TCCAGGAAGTGCCTCTCTGCAGCTGCCGAATGGAAACCCCCAAGAGCCGCGAGATCAGCACCCTGGCCAA
  CAACCAGTGCATGGCCACTGAGAGCGTGGATCACGAAGGTAACTTCATGGAATGCCAGCCTGAGAGCAGC
  ATCTCTCATCGTTTCCATAAGGACTGTGCCTCTCGAGTCAACAATGCCAGCTACTGTCCCCATTGTGGGG
  AAGAAGCTTCCAAGGCCAAAGAGGTGACCATAGCAAAAGCAGACACAACTTCCACAGTGACCCTAGCCCC
  TGGACAGGAGAAGAGCCTGGCTGCTGAAGGCAGGGCTGACACGACCACGGGCAGCATTGCTGGAGCCCCA
  GAGGATGAAAGATCGCAGAGCACAGCCCCCCAGGCACCAGAGTGCTTCGACCCTGCCGGACCGGCTGGGC
  TCGTGAGGCCGACATCTGGCCTTTCCCAGGGCCCAGGAAAGGAAACCTTGGAAAGTGCTCTAATCGCTCT
  AGACTCTGAAAAACCCAAGAAACTTCGCTTCCACCCAAAGCAGCTGTACTTCTCTGCCAGGCAGGGTGAG
  CTGCAGAAGGTGCTTCTCATGCTGGTTGATGGAATTGATCCCAACTTCAAAATGGAGCACCAAAGTAAGC
  GTTCCCCATTACATGCTGCTGCGGAGGCTGGCCACGTGGACATCTGCCACATGCTGGTTCAGGCGGGTGC
  CAATATTGACACTTGCTCAGAGGACCAGCGGACCCCACTGATGGAGGCTGCAGAGAACAACCACTTGGAT
  GCAGTGAAGTACCTCATCAAGGCTGGAGCACAGGTGGATCCGAAGGACGCAGAGGGCTCCACATGTTTGC
  ATTTGGCTGCCAAGAAAGGCCACTATGATGTGGTTCAGTATCTGCTTTCAAATGGACAGATGGATGTCAA
  CTGCCAGGATGACGGTGGATGGACACCTATGATCTGGGCCACTGAGTACAAGCACGTGGAGCTGGTGAAG
  CTGCTGCTGTCTAAGGGCTCTGACATCAACATCCGGGACAACGAGGAGAACATTTGTCTGCACTGGGCAG
  CATTTTCAGGCTGTGTGGACATAGCTGAAATACTTCTGGCTGCCAAGTGTGACCTGCATGCTGTGAATAT
  CCATGGAGACTCACCCCTGCACATCGCAGCCAGGGAGAATCGCTACGACTGTGTTGTCCTCTTTCTTTCT
  CGGGATTCAGATGTTACTCTGAAAAACAAGGAAGGAGAGACTCCCTTGCAGTGTGCAAGTCTCAGTTCGC
  AGGTGTGGAGTGCATTGCAGATGAGCAAAGCACTTCGGGACTCAGCCCCTGACAAGCCCGTTGCTGTTGA
  GAAGACGGTGAGCAGGGATATCGCTCGAGGGTATGAGCGCATTCCCATTCCCTGTGTCAATGCTGTGGAC
  AGTGAGCTGTGTCCTACCAACTATAAGTATGTCTCCCAGAACTGTGTGACATCCCCCATGAACATTGACA
  GGAACATCACTCATTTGCAGTACTGCGTGTGTGTAGATGACTGCTCCTCTAGCACCTGCATGTGTGGCCA
  GCTGAGCATGCGCTGCTGGTATGATAAGGATGGCCGACTTCTGCCTGAGTTTAACATGGCAGAACCACCC
  TTGATCTTCGAGTGCAATCATGCCTGCTCATGCTGGAGGAACTGCCGCAATCGTGTGGTGCAAAATGGTC
  TCAGGGCAAGGCTGCAGCTTTATCGGACACAGGACATGGGCTGGGGTGTGCGGTCCCTCCAGGATATCCC
  ACTGGGCACCTTTGTCTGCGAATACGTAGGGGAGCTGATTTCGGACTCTGAAGCTGATGTTCGGGAAGAG
  GACTCTTACCTCTTTGATCTTGACAATAAGGATGGAGAGGTATACTGCATTGACGCTCGGTTCTATGGGA
  ATGTCAGCCGGTTCATAAACCACCACTGCGAACCCAACCTTGTGCCTGTGCGAGTGTTCATGTCACACCA
  GGACCTGCGGTTTCCCAGGATTGCCTTCTTCAGTACCCGCCTGATTCAGGCTGGGGAGCAGCTCGGGTTC
  GACTACGGGGAGCGCTTTTGGGACGTCAAGGGCAAGCTCTTCAGTTGCCGGTGTGGGTCTTCCAAGTGTC
  GGCACTCAAGCGCAGCCCTGGCCCAGAGGCAAGCCAGTGCAGCCCAGGAGCCTCAGGAGAATGGCCTTCC
  AGATACCAGCTCTGCAGCCGCTGCTGACCCCCTATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001109687
• Insert Size: 3747 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001109687.2, NP_001103157.1
• RefSeq Size: 4963 bp
• RefSeq ORF: 3747 bp
• Locus ID: 77683
• UniProt ID: Q5DW34
• Cytogenetics: 2 A3
• Gene Summary: Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (4) is shorter than isoform 1.