Cilp (NM_173385) Mouse Untagged Clone

CAT#: MC224053

Cilp (untagged) - Mouse cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (Cilp), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cilp
• Synonyms: 9830114D16; C130036G17Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224053 representing NM_173385
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCAATCAAGACTTGGGTATTCTCTTTCCTGGTCCTTGAGGTCACCACCGTGCTGGGGAGACAGA
  CGATGCTTGCCCAGTCAGTGAGAAGAGTCCAGCCTGTGAAGAGGACCCCCAAGACCCTTGCCAAGCCTGC
  CGACTCCCAGGAGAGTCCCGGAGAGTGGACAACATGGTTTAACATCGACCACCCAGGTGGGCAGGGTGAC
  TATGAGCGGCTGGATGCCATTCGCTTCTACTATGGGGAGCGTGTGTGTGCCCGTCCCCTGCGGCTAGAGG
  CCCGGACCACTGACTGGATGCCTGCGGGCAGCACTGGCCAGGTGGTCCACGGCAGTCCCCGTGAGGGCTT
  CTGGTGCCTCAACAGGGAGCAGAGACCAGGCCAGAACTGTTCCAATTATACCGTGCGCTTCCTCTGCCCA
  CCAGGGTCCTTGCGAGGAGATGCAGAGCACATCTGGAGTTCCTGGTCTCCCTGGAGCAAGTGCTCAGCTG
  CCTGCGGTCACACTGGGGTGCAGACCCGTACCCGCACCTGCTTGGCACAGACAGTGTCACTGTGCAGTGA
  GGCGACCGAGGAGGGCCAGCTCTGCATGAGCCAGGCCTGCACAGCTTGTGACCTGACCTGCCCCATGGGC
  CAGGTAAATGCTGACTGTGATGCCTGCATGTGCCAGGACTTCATGCTTCACGGGGCCATCTCCCTCCCTG
  GAGGTGGCCCAGCTCCAGGAGCCGCTGTCTACCTGCTGGCCAAGGCACCAAAGATGCTGACCCGAACAGA
  CAGCAGCGGGAGGTTCCGAGTTCCTGGCTTGTGTCCTGATGGCAAAACCATCCTGAAAATCACCAAGACC
  AAGTTTGCCCCAATTATGATCACGATGCCCAAGACTAGCCTGAAGTCAGCTACCATCAATGCGGAGTTTG
  TGAGGGCAGAGACCCCATACATTGTAATGAACCCTGAGATGAAAGCACGTCGGGCTGGGCAGAGTGTGTC
  TCTGTGCTGCAAAGCCACGGGGAAACCCAGTCCGGACAAGTACTTCTGGTACCACAACAACACACTGCTG
  GATCCCTCCCTCTATAAGCACGAGAGCAAGCTGGTGCTGAGGAACCTGCAGCAGGACCAAGCAGGGGAGT
  ACTTCTGTAAGGCTCAGAGTGATGCTGGGGCTGTGAAGTCCAAGGTCACCCAGCTCACTGTCATTGCACA
  TGATGAGACTCCTTGCAACCCAACCCCAGAGAGCTACCTTATCCGGCTGCCCCATGACTGCTTTCAGAAC
  GCCAGCAATTCCTTCTACTACGACGTAGGCCGCTGCCCGATCAAGACCTGTGCAGGGCAGCAGGACAATG
  GGATCCGGTGCCGAGATGCTGTGGAGAACTGCTGCGGCATTTCCAGAACAGAAGAGAGGGAGATCCAGTG
  CAGTGGGTACACACTGCCCACCAAGGTGGCTGTGGAGTGCAGCTGCCAACGATGCGCAGAGACACGGAGC
  ATCGTCCGGGGCCGAGTCACTGCTGCTGACAACGGGGAGCCCATGCGCTTTGGCCATGTGTACATGGGGA
  ATAACCGCGTGAGCATGACTGGCTACAAAGGCACCTTCACACTCCACATCCCCCAGGACACGGAGAGGCT
  GGTGCTCACATTTGTGGACAGGCTGCAGAAGTTTGTCAACACAACCAAAGTGCTGCCCTTCAACAAGAAG
  GGAAGCGCAGTGTTCCACGAGATCAAGATGCTTCGGCAGAAAGAGCCCATCACGTTGGAAGCCATGGAGA
  CCAACATTATCCCCCTGGGAGAGGTGATTGGTGAAGACCCTGTGGCTGAGCTGGAGATCCCATCCAAAAG
  TTTCTATAGGCAGAACGGGGAGCCCTTCACAGGAAAGGTAAAGGCCAGCGTGACCTTCCTGGATCCTCGG
  AACATCTCGACGGCCACAGCTGCCCAAAGTGACCTCAACTTCATCAATGATGAAGGAGACACTTTTCCCC
  TTAGAACATATGGTATGTTTTCAGTGGACTTTAGAGATGAGGCCACTTCCGAGTCACTTAATGCTGGCAA
  GGTGAAGGTCCACCTTGACTCGACCCAGGTCAAAATGCCAGAGCATGTGCCAGCCATGAAACTCTGGTCG
  CTCAACCCAGACACAGGACTGTGGGAAGAGGAAGGGGATTTCAAGTTTGAAAGCCAAAGGAGGAACAAGA
  GGGAAGAGAGAACCTTCCTAGTGGGCAATATGGAGATCCGGGAGAGAAGGCTCTTTAACCTGGACGTCCC
  TGAAAGCCGAAGGTGCTTCATTAAGGTGAGGACTTACCGGAGTGAGAGGTTCCTGCCCAGTGAGCAAATT
  CAGGGAGTTGTGGTCTCAGTGATCAATCTGGAGCCCAGAACTGGCTTCTCATCTAACCCCAGGGCCTGGG
  GCCGATTTGACAGTGTCATCACAGGGCCCAATGGGGCCTGCCTGCCTGCCTTCTGTGATGATCAGTCCCC
  CGATGCTTACTCTGTCTATGTCTTGGCAAGCCTTTCTGGAGAAGAACTAGAGGCAGTTGAGTCTTCTCCT
  AAATTCAACCCAAATGCAATCGGTGTCCCTCAGCCTTACCTCAACAAACTTAAATACCGTCGGACAGACC
  ATGAGGATCCACGGGTTAAAAAGACGGCTTTCCAAATCAGTATGGCCAAACCAAGGCCCAACTCAGCTGA
  GGAGAGCAATGGGCCTATCTATGCCTTTGAGAACCTTCGGGCGTGTGAGGAGGCACCCCCCAGTGCAGCT
  CACTTCCGGTTCTATCAGATCGAGGGGGATCGCTATGACTATAACACAGTTCCTTTCAATGAAGATGACC
  CTATGAGCTGGACTGAAGACTACCTGGCATGGTGGCCCAAGCCAATGGAGTTCAGGGCCTGCTATATCAA
  GGTTAAGATTGTGGGGCCACTGGAGGTGAATGTACGATCCCGTAACATGGGAGGCACCCACCGGCAGACT
  GTGGGGAAGCTCTATGGGATCCGGGATGTGAAGAGTACTCGGGACAGAGACCAACCTAATGTCTCATCTG
  CCTGCCTGGAGTTCAAGTGCAGTGGAATGCTGTATGACCAAGACCGTGTAGACCGCACATTAGTAAAGGT
  TATCCCCCAGGGCAGCTGCCATCGAGCTAGTGTTAACTCTATGTTGCACGAGTACCTGGTCAACCACCTT
  CCCCTGGCAGTCAACAATGACACCAGCGAGTATACTATGCTGGCACCCCTGGACCCTCTGGGCCACAATT
  ACGGTATCTATACTGTTACTGATCAGGACCCTCGTACAGCTAAGGAGATTGCGCTCGGCCGGTGCTTTGA
  TGGCACTTCCGATGGCTCCTCCAGAATCATGAAGAGCAATGTGGGAGTTGCCCTGACCTTTAACTGTGCA
  GAAAGGCAGGTAGGCCGCCAGAGTGCCTTCCAGTACCTCCAAAGCACACCGGCCAGGTCCCCAGCTACAG
  GCACTGTCCAAGGAAGAGTACCAGCCATGAGGCAACAACGGGCAAGCAGGGGTGGCCTACGCCGGCGTGG
  AAGCATGGGCCCCTCTGAGATTTTCTGGTGTTGCTCAACAACCTCTGAGCAACTAAGTCTTGTGGTTCTT
  AGTCCTTTCTGTCCATCTCATGTGACAGCTGTCGTGAGACTGATGCACAGACTGTCACTTGTTAATTTAA
  ACATATCTGGTTTGGTGCAGTTTGTTTCTTTGTGCCTTTACTTACTGTCTCTGCCTCATGATGCTGATTG
  CCACAGGGCCCCCACGATGACAAAACAAAGATTCTCCTCTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_173385
• Insert Size: 3753 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173385.2, NP_775561.1
• RefSeq Size: 4153 bp
• RefSeq ORF: 3753 bp
• Locus ID: 214425
• UniProt ID: Q66K08
• Cytogenetics: 9 C
• Gene Summary: Probably plays a role in cartilage scaffolding. May act by antagonizing TGF-beta1 (TGFB1) and IGF1 functions. Has the ability to suppress IGF1-induced proliferation and sulfated proteoglycan synthesis, and inhibits ligand-induced IGF1R autophosphorylation. May inhibit TGFB1-mediated induction of cartilage matrix genes via its interaction with TGFB1. Overexpression may lead to impair chondrocyte growth and matrix repair and indirectly promote inorganic pyrophosphate (PPi) supersaturation in aging and osteoarthritis cartilage (By similarity).[UniProtKB/Swiss-Prot Function]