Camsap3 (NM_027171) Mouse Untagged Clone

CAT#: MC224050

Camsap3 (untagged) - Mouse RIKEN cDNA 2310057J16 gene (2310057J16Rik), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Camsap3
• Synonyms: 2310057J16Rik; Kiaa1543; Nezha
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224050 representing NM_027171
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGAAGCGGCGCCCGCAGGGTCCGGGCCACTGCGGAGGACTTTCCTGGTCCCCGAGATCAAGTCTT
  TGGACCAGTACGATTTCTCTCGAGCCAAGGCGGCGGCGAGTCTGGCGTGGGTGCTGCGGGCCGCATTCGG
  GGGAGCAGAGCATGTGCCTCCGGAGCTGTGGGAACCCTTCTACACCGATCAGTACGCACAGGAGCACGTG
  AAGCCCCCAGTGACGCGGCTGCTGCTCTCTGCGGAACTATACTGTCGGGCCTGGCGCCAGGCACTGCCAC
  AGCTCGAGCCATCCCCCAGTCCCTCTGCTCTGTTGGCCTTGCTGGCGAGGAGGGGCACGGTGCCTTCACT
  GCCTGAGCACCCAGTGCGCGAGGCTGACCTGAAACACCAGCCAATTCTCATGGGAGCCCACCTAGCTGTC
  ATCGATGCTCTCATGGTTGCCTTCTCATTTGAGTGGACAAAGACACTACCTGGTCCCTTGGCTCTGAGCA
  GCTTGGAGCACAAACTCCTTTTCTGGGTAGACACAACTGTTCGGCGGCTGCAGGAGAAGACAGAACAAGA
  AGCAGCCCAGCGTGCATCTCCTGCAGCCCCTCTGGATGGGGCCTCTCCTGCCCAGCCCTCGATTCGATAT
  CGCAAGGACCGTGCCATTGCCCGAAGGGCTCCCTGCTTTCCAAATGTGACTACCCTTCAGGACCTGGCCA
  GTGGGGCAGCACTGGCTGCCACCATCCACTGCTATTGTCCCCAGCTATTACGACTTGAGGAGGTGTGCCT
  CAAGGACCCCATGTCTGTGGCAGACAGTCTATACAACCTCCAGCTGGTGCAAGACTTCTGTGCCTCCCAT
  CTTCCTCGAGGCTGCCCCCTGTCCCTTGAAGACTTGCTGTATGTCCCACCACCCCTCAAGGTCAACCTGG
  TGGTGCTGTTGGCTGAGATGTATATGTGCTTTGAGGTTCTGAAGCCTGATTTTGTGCAGGCCAAAGACTT
  GCCTGATGGACATGCTGTCTCCCCCCGGAATACAGAGACTGTTCCATCTCAGAACAACAGTGGCAGCAGT
  TCTCCTGTCTTCAACTTCCGTCACCCACTTCTGTCACCTGGTGGTCCCCAGTCTCCACTCCGAGGATCCA
  CAGGCTCCCTGAAGTCCTCTCCATCAATGTCTCACATGGAGGCTCTTGGCAAAGCCTGGAACCGTCAGCT
  TAGCCGTCCCCTCTCCCAGGCTGTGTCGTTCAGCACTCCCTTTGGCCTGGACAGCGATGTGGATGTCGTC
  ATGGGAGATCCTGTCCTGCTCCGCTCCGTCAGCTCAGACAGTCTGGGTCCTCCACGTCCTGTGTCAACAT
  CATCCCGGAATTCTGCTCAGCCAGCCCCAGAATCTGGAGACCTACCCACGATTGAAGAGGCCCTGCAGAT
  CATTCACAGTGCTGAGCCCCGACTGCTCCCTGATGGGGCTGCTGATGGCAGTTTCTACCTCCATTCTCCT
  GAGGGTCTCTCCAAACCACCACTCTCCCCCTACCCTCCCGAAGGAGCCTCAAAGCCGCTGTCTGATAGGC
  TCAACAAGGCGCCTATCTATATATCACACCCTGAGAACCCTTCAAAATCATCTCCCTGCTCAACAGGAGA
  GATACTGAAACCACCACCCCCATCCGAGGGTTCCCCCAAAGCTGTGGCTTCATCCCCAGCAGCCAACAAC
  TCCGAAGTGAAGATGACCAGTTTTGCTGAACGCAAGAAACAGCTGGTGAAGGCTGAGGCTGAGTCAGGAT
  TGGGGTCTCCAACATCCACCCCCGTAGCACCTGAGGCCTTGAGCTCAGAGATGAGTGAGCTGGGAGCCAG
  GCTGGAGGAGAAGCGCCGAGCCATAGAGGCACAGAAACGACGCATTGAGGCAATCTTTGCCAAGCATAGG
  CAGAGGCTGGGCAAGAGCGCTTTCCTGCAGGTGCAGCCTCGGGAGGCTGCAGGGGAGGCCGAGGAGGAAG
  CTGAGCTGGGCTCAGTTCCTGGTGGGGAACGGCCAGCAGGTGAGGGCCAGGGTGAGCCGTCCTTACGGCA
  CAAGTCAGTTACCTTCTCTCCAGACCTGGGCCCAGTGCCCCCAGAGGGACTTGGGGATTACAATAGAGCA
  GTCAGTAAGCTGAGTGCCGCTCTGAGCTCTCTGCAGCGGGACATGCAGAGGCTCACAGACCAGCAACAGC
  GGCTTCTAGCCCCTCCAGAAGCTCCTGGACCTGCCCCACCACCTGCAGCCTGGGTCATTCCTGGACCCGC
  CACTGGGCCTAAAGCAGCATCCCCCAGCCCTGCCCGTCGTGCCCCAGCTGCCCGACGCAGCCCTGGGCCA
  GGCCCCAGCCCAACACCCCGTAGTCCAAAACATGCAAGGCCGGCAGAGCTGAAGCTTGCACCTTTGACAA
  GGGTACTCACACCACCCCATGATGTAGACAGCCTCCCTCACCTACGCAAGTTTTCACCCAGCCAGGTGCC
  TGTACAGACTCGCTCCTCTATCCTCCTGTCGGAGGGGACACCTCCCGAGGAGCCCACCACCAAGCCTGCC
  CTCATTGAGATCCCTCTAGCCAGCCTGGGGGAGCCTGCTGCTGATGAGGAAGGAGATGGGAGCCCCCCTG
  GGGCTGAGGATTCCTTAGAGGAAGAGGCATCTTCTGAGGGAGAGCCCCGATCAGGGCTTGGATTCTTTTA
  TAAGGACGAAGACAAGCCTGAGGATGAGATGGCTCAGAAGCGAGCTAGCCTGCTGGAGCGTCAGCAGAGG
  CGGGTAGAGGAAGCCCGGCGGCGCAAACAGTGGCAGGAGGCAGAGAAGGAGCAGAAACGGGAGGAGGCCG
  CCAGGCTGGCTCAGGAGGCTCCAGGCTTGGCCTTTACAACTCCTGTTGTAGCCTCTGCGGCTCCAGTGGC
  CACCTTGGCTCCTACTACCAGAGCCATGGCCCCAGCTGAGGAGGAGGTGGGCCCCCGGCGGGGGGACTTC
  ACAAGACTTGAGTATGAACGCCGGGCACAACTGAAACTGATGGATGACCTTGATAAGGTGCTACGGCCCC
  GGGCCTCAGGGACCGGGGGACCAGGGCGGGGCGGGCGCAGGGCCACCCGGCCACGCTCTGGTTGCTGTGA
  TGACTCGGCCTTGGCACGAAGCCCAGCCCGCGGCCTGCTGGGTTCACGGCTCAGCAAGGTCTATTCCCAG
  TCCACGCTGTCCCTATCTACGGTGGCCAACGAGGCTCCCAATAACCTTGGTGTGAAGAGGCCCACCTCTC
  GGGCCCCTTCTCCATCAGGCCTCATGTCTCCAAGCCGCCTGCCTGGCAGTCGAGAACGTGACTGGGAGAA
  TGGAAGCAATGCATCCTCCCCAGCATCAGTGCCTGAGTACACAGGTCCCCGGCTATACAAGGAACCCAGC
  GCCAAGTCTAACAAGTTTATCATCCACAATGCCTTGTCACACTGCTGCCTGGCAGGCAAGGTGAATGAGC
  CCCAGAAGAACAGAATTCTAGAGGAAATCGAGAAAAGCAAGGCCAACCACTTCCTGATTCTCTTTCGGGA
  CTCAAGCTGCCAGTTCCGGGCCCTCTACACTCTGTCTGGGGAGACAGAGGAGCTATCGAGGCTGGCAGGC
  TATGGGCCTCGGACCGTCACTCCTGCCATGGTCGAAGGCATCTATAAGTACAACTCGGATCGCAAACGGT
  TCACCCAGATCCCTGCTAAAACCATGTCTATGAGTGTAGACGCCTTCACTATCCAGGGACACCTTTGGCA
  AAGCAAGAAGCCCACCACGCCCAAGAAGGGTGGCGGTACCCCCAAATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027171
• Insert Size: 3759 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027171.3, NP_081447.2
• RefSeq Size: 4303 bp
• RefSeq ORF: 3759 bp
• Locus ID: 69697
• UniProt ID: Q80VC9
• Cytogenetics: 8 A1.1
• Gene Summary: Key microtubule-organizing protein that specifically binds the minus-end of non-centrosomal microtubules and regulates their dynamics and organization (PubMed:23169647, PubMed:24706919, PubMed:26715742). Specifically recognizes growing microtubule minus-ends and autonomously decorates and stabilizes microtubule lattice formed by microtubule minus-end polymerization (PubMed:24706919). Acts on free microtubule minus-ends that are not capped by microtubule-nucleating proteins or other factors and protects microtubule minus-ends from depolymerization (PubMed:24706919). In addition, it also reduces the velocity of microtubule polymerization (PubMed:24706919). Required for the biogenesis and the maintenance of zonula adherens by anchoring the minus-end of microtubules to zonula adherens and by recruiting the kinesin KIFC3 to those junctional sites (By similarity). Required for orienting the apical-to-basal polarity of microtubules in epithelial cells: acts by tethering non-centrosomal microtubules to the apical cortex, leading to their longitudinal orientation (PubMed:26715742). Plays a key role in early embryos, which lack centrosomes: accumulates at the microtubule bridges that connect pairs of cells and enables the formation of a non-centrosomal microtubule-organizing center that directs intracellular transport in the early embryo (PubMed:28860385). Couples non-centrosomal microtubules with actin: interaction with MACF1 at the minus ends of non-centrosomal microtubules, tethers the microtubules to actin filaments, regulating focal adhesion size and cell migration (By similarity). Plays a key role in the generation of non-centrosomal microtubules by accumulating in the pericentrosomal region and cooperating with KATNA1 to release non-centrosomal microtubules from the centrosome (By similarity). Through the microtubule cytoskeleton, also regulates the organization of cellular organelles including the Golgi and the early endosomes (By similarity). Through the microtubule cytoskeleton, also regulates the organization of cellular organelles including the Golgi and the early endosomes (By similarity). Through interaction with AKAP9, involved in translocation of Golgi vesicles in epithelial cells, where microtubules are mainly non-centrosomal (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks two exons in the 5' coding region compared to variant 3. The encoded isoform (2) is shorter than isoform 3.