Spata13 (NM_001033272) Mouse Untagged Clone

CAT#: MC224039

Spata13 (untagged) - Mouse spermatogenesis associated 13 (Spata13), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Spata13
• Synonyms: C79050; ESTM11; mFLJ00298; X83327
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224039 representing NM_001033272
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAACAGCCCAGGATGGCCATGGGCAAGGTGCCACAGCCACTTGTTCAGCCTCCAACCCAAAGGCCC
  CGAAAATGGTGACATCCTCAGCATGTCAGAATGGAGGCTGCAAAAGCAGCCCCAGCAGTGATCCCGAAGC
  CGGGGAAACCAGGCTCAGCCCATCGAAGCTGGTACGTCTCTTCTCTGGTAGTCGGAAGAGGATGAATTCG
  AACTCTGAGAGACCTCGCTCAGTCGTCCTTGTGGGGAATTCCTCCACGTGGAATGCCCTGGCCTCCTTCC
  GTAAAATGGGCTCCTTTAAAAGACTAAAATCTTCTGTCCTTCAAGGAATTCAGAACCGGGAGGGGGCAGA
  AGTTTCCAAGGAGGAGCCTACAGGAGACCCAGGAAGGGCTGCTCCCAATGGCACCGTTGCCATCCTGGGA
  AAGGGCCTGGGCAGGTGTACATCCTTGGGCCCTGTAGGAGATGGCGCCGGGTCTGACTGCTCAGACCCCG
  AGGACACGGAGGACGCCTTCCAGAGGAGCACACACCGTTCTCGCAGCATCCGCAGGGCCTATGGCCTGGG
  CCGCATCAGCCTGCTGGACCTGGGGCGGCAGCCAGTGCCGGAGCCCGCCGTGTGTGAGATCCAAGTTCGA
  GACCCTGAACCCAGCAGGGCTGTGCCTCTGCCACGCAGAAGCAAGAGCATCGACAGCTTGAGCGTCCTGA
  AGAGCTCCTTCAAGCGCAAGTCTGCATCCAACCTCACGGAACTGCAGGGTGACAGGCAGGTTCCCTCCAG
  AACCTTGAGCAGCTTCTTCACAGACCCGGAGAAGCCGGGTGGCTCAGAGAGAAGAACCAGACGCTGGAGG
  AGCCCCATCCGGGCCAAGGACTTCGACAGAGTCCTGAGACTGGTGAGCAATGTCAAGGATGCGGCTTGGA
  AGAGGGAGGCCCCCAGGAGTGCAGCCCCTGGCCCTGGTCCCGGTCTCGGGGATGAGAACCCACTACTTGC
  GCCACGAAGCAAGCTACACGATGACTACTCGCGTCGTACTAGCAGCAGCGTGGAGCCAGATGCCAGGCGT
  GGCGGCTCACCCTGTATCCCTGCACCATGCACCACTGCGCCCTACGCAGCTCCAGAGCCCCACTTAGATG
  TGGATACTGCAGTATTCCCCCTGGAAACTAAAAGTGCTCAGCCACTGGGAAATGATGGACCTCGTGCCAG
  CAGTCCCTCACCAATCCCCACGGACCCAGAAGGGTTGAGCCAAGGCTCCAGTGAGGCACATACTGGCAGC
  CAACATCCTTTTAATCCCGTGCAGCTTCCCACTCCTCTGAGACCTACCACACCCAAGCCCCAAAGCCCTC
  AGAGCCCAGGGAGTACTAAGTGCCCCAGTAGTCTGAGTGTGCTGTCCCTCAGTTCAGCGGACAGTGAAGA
  GCGGACTGAGGATGCTTCGCACAGGCAACCGGGACCCGTGTCCCTCCAGGATACTGTCCTTAACGCTGCT
  GGTGATGTGGGGGGAGAAAGCGGTGACAGCTATTTTCCCGAGAATCTCGATGGAGCCACCCATCCAGAAG
  AGAAGAAGGAAGAGGTCCTGGTGACTGAGGATGACTGGCCGCAGGGCTCAGCCCTGGAGGAGGAGCAGAA
  TGAGGCGCCTCGAATCTCCAGGAGGAGGTGGGGCTCGGGACGCCGCACCCGGCCACGACCACTGTCTGAC
  TATGGCCAGCTGGCCGGCAGAAGCTTGTCCATTCCTGAAGATGCCATTGCTGCAGACCCTCCAGATGAGG
  ACCACGTGGACAGGATGCACCCAGCAAGTGTGACTACCACCAGCCAGGATCCATGCGCCCCCTCAGGCTC
  TTGCAGGGGAGGCCGGAGAAGGCGACCTATATCTGTGATTGGAGGGGTCAGCTTCTATGGCAACACCCAG
  GTAGAGGATGTGGAGAACCTCTTGGTCCAACCAGCAGCCAGGCCTCCTGTGCCTGCACACCAAGTTCCAC
  CCTACAAGGCTGTGTCTGCCCGCCTCCGGCCCTTCACCTTCTCTCAGAGCACTCCCATCGGGCTGGACCG
  CGTGGGGCGGCGACGGCAGATGAAAACATCCAATGTCTCTTCAGATGGAGGTGCGGAGTCTTCGGCCTTA
  GTGGATGATAATGGTAGTGAGGAAGACTTCAGCTATGAGGAGCTCTGCCAGGCCAACCCTCGGTACCTGC
  AGCCAGGAGGGGAACAGCTGGCCATCAATGAGCTCATCAGCGATGGCAGCGTGGTCTGTGCAGAAGCACT
  GTGGGACCATGTGACCATGGACGACCAGGAGCTGGGCTTCAAGGCTGGGGATGTCATCCAAGTTCTGGAA
  GCTTCTAACAAGGACTGGTGGTGGGGCCGGAATGAAGACAAAGAGGCCTGGTTCCCTGCAAGCTTTGTTA
  GGCTTCGAGTCAATCAGGAGGAGCTGCCCGAGAACTGCAGCAGCTCCCATGGGGAAGAGCAGGATGAGGA
  CACCAGCAAAGCCCGCCACAAGCACCCAGAGAGCCAGCAGCAGATGCGGACCAACGTCATCCAGGAGATA
  ATGAACACCGAGCGAGTGTATATCAAGCACCTGAAGGACATCTGCGAGGGCTATATCCGACAGTGTCGTA
  AGCACACGGGAATGTTCACCGTTGCGCAGCTAGCCACTATTTTTGGAAACATTGAAGACATTTACAAATT
  CCAAAGAAAGTTTCTGAAAGACCTTGAGAAACAGTACAACAAAGAGGAACCTCACTTGAGTGAAATAGGA
  TCCTGCTTTCTTGAGCACCAAGAGGGCTTCGCCATCTACTCTGAGTACTGCAACAACCACCCAGGCGCCT
  GTGTGGAGCTGTCCAACCTCATGAAACACAGCAAGTACCGGCACTTCTTCGAGGCCTGCCGCCTGCTCCA
  GCAGATGATCGACATCGCCCTGGACGGCTTCCTCCTCACGCCTGTGCAGAAGATCTGCAAATACCCGCTG
  CAGCTGGCTGAGCTGCTCAAGTACACCACACAGGAGCACGGCGATTACAACAATATAAAGGCAGCCTATG
  AAGCCATGAAGAACGTGGCCTGCTTGATCAATGAACGCAAGCGCAAGCTGGAGAGCATCGACAAGATCGC
  CCGCTGGCAGGTGTCCATCGTAGGCTGGGAGGGCCTGGACATTCTAGACCGAAGCTCTGAGTTGATTCAT
  TCCGGAGAGCTGACCAAAATCACCAGGCAGGGCAAGAGCCAGCAACGCATCTTCTTCCTTTTCGACCACC
  AGCTGGTGTCCTGCAAGAAGGACCTGCTGCGCAGGGACATGCTATACTACAAGGGCCGCATGGACATGGA
  CGAGGTAGAGCTTGTGGACGTGGAGGATGGGCGGGACAAAGACTGGAGCCTCAGCCTGCGGAATGCCTTC
  AAGCTGGTCAGCAAAGCCACGGACGAGGTCCATCTGTTCTGCGCCAGAAAGCAAGAAGACAAGGCCAGGT
  GGCTGCAGGCCTATGCGGACGAGAGGCGGCGGGTGCAGGAGGACCAGCAGATGGGTATGGAAATCCCAGA
  AAATCAGAAGAAACTCGCCATGTTGAACGCTCAGAAGGCAGGACATGGGAAATCTAAAGGCTACAACAGC
  TGTCCCGTGGCCCCACCCCACCAGAGCCTGCCGCCCCTTCACCAGCGCCACATCACTGTGCCCACCAGCA
  TCCCGCAGCAGCAGGTCTTTGCCTTGGCTGAGCCCAAGAGGAAGCCATCGATATTCTGGCACACTTTCCA
  CAAACTCACCCCCTTCCGGAAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001033272
• Insert Size: 3735 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033272.2, NP_001028444.1
• RefSeq Size: 7557 bp
• RefSeq ORF: 3735 bp
• Locus ID: 219140
• UniProt ID: Q5DU57
• Cytogenetics: 14 31.77 cM
• Gene Summary: Acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Regulates cell migration and adhesion assembly and disassembly through a RAC1, PI3K, RHOA and AKT1-dependent mechanism. Increases both RAC1 and CDC42 activity, but decreases the amount of active RHOA (By similarity). Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.