Grin2c (NM_010350) Mouse Untagged Clone

CAT#: MC224027

Grin2c (untagged) - Mouse glutamate receptor, ionotropic, NMDA2C (epsilon 3) (Grin2c), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Grin2c
• Synonyms: GluN2C; NMDAR2C; NR2C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224027 representing NM_010350
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGGAGCCCTGGGGCCCGCCCTGCTTCTCACTTCACTCCTTGGTGCTTGGGCAGGGCTGGGCGCAG
  GGCAGGGAGAACAGGCCGTGACCGTGGCGGTGGTGTTTGGCAGCTCTGGGCCACTGCAGGCCCAGGCCCG
  GACTCGTCTCACCCCGCAGAACTTCCTGGACTTGCCTCTGGAGATCCAGCCACTCACCATCGGGGTCAAC
  AATACCAACCCCAGCAGCATCCTCACCCAAATCTGTGGGCTCCTGGGTGCCGCCCGAGTCCACGGCATCG
  TCTTTGAGGACAACGTGGACACTGAGGCCGTGGCTCAGCTGCTGGATTTCGTCTCCTCTCAGACCCACGT
  GCCCATCCTCAGCATCAGTGGAGGTTCTGCTGTGGTCCTCACCCCCAAGGAGCCAGGCTCCGCCTTTCTA
  CAGCTGGGCGTGTCCCTGGAGCAGCAGCTGCAGGTGCTGTTCAAGGTGCTGGAGGAATACGACTGGAGCG
  CGTTCGCTGTCATCACCAGCCTGCACCCGGGCCACGCGCTCTTCCTCGAGGGCGTGCGCGCCGTCGCCGA
  CGCCAGCTACCTGAGCTGGCGGCTGCTGGACGTGCTCACGCTGGAGCTGGGCCCCGGTGGGCCGCGAGCG
  CGCACTCAGCGCTTACTGCGCCAGGTCGACGCCCCGGTGCTGGTGGCCTACTGCTCCCGTGAAGAGGCGG
  AGGTGCTCTTCGCGGAGGCTGCACAGGCTGGCTTGGTGGGACCCGGTCACGTGTGGTTAGTACCTAATCT
  GGCGCTGGGAAGCACCGACGCTCCCCCTGCAGCCTTCCCAGTGGGCCTCATCAGTGTGGTCACCGAGAGT
  TGGCGCCTTAGCCTACGCCAGAAAGTCCGCGACGGTGTAGCCATTCTGGCCCTCGGTGCCCACAGCTACC
  GACGCCAGTACGGTACCCTTCCAGCCCCGGCTGGAGACTGCCGAAGCCACCCAGGACCCGTCAGCCCTGC
  CAGGGAGGCTTTCTACAGGCATCTGCTGAATGTCACCTGGGAAGGCCGAGACTTCTCTTTTAGCCCTGGT
  GGGTACCTGGTCCAGCCCACAATGGTTGTGATCGCTCTCAACCGGCATCGCCTCTGGGAGATGGTGGGAC
  GGTGGGATCATGGGGTCCTGTACATGAAGTATCCAGTATGGCCTCGCTACAGCACTTCTCTGCAGCCTGT
  GGTGGACAGCCGGCACCTGACAGTGGCCACACTGGAAGAAAGGCCTTTTGTCATTGTGGAGAGCCCTGAC
  CCTGGCACAGGTGGCTGTGTTCCCAACACTGTGCCCTGCCGTAGACAGAGCAACCACACCTTCAGCAGCG
  GGGATATAACCCCCTACACCAAGCTCTGTTGTAAGGGCTTCTGCATCGACATCCTCAAGAAGCTGGCCAA
  GGTGGTCAAGTTCTCCTACGACTTGTACCTGGTGACCAACGGCAAGCACGGCAAGAGGGTTCGTGGTGTG
  TGGAATGGTATGATCGGTGAGGTATACTACAAGCGGGCAGACATGGCCATCGGCTCCCTCACCATCAATG
  AAGAGCGCTCAGAGATTATAGACTTCTCTGTGCCTTTTGTGGAGACCGGCATCAGTGTGATGGTGGCAAG
  GAGCAACGGCACCGTCTCCCCCTCGGCTTTTCTGGAGCCCTACAGCCCTGCCGTGTGGGTGATGATGTTT
  GTAATGTGCCTCACGGTGGTTGCCATCACTGTCTTCATGTTCGAGTATTTCAGCCCTGTCAGCTACAACC
  AGAATCTCACCAAGGGCAAGAAGTCAGGTGGACCATCCTTCACCATTGGCAAGTCCGTGTGGTTGCTGTG
  GGCACTGGTCTTCAACAACTCTGTTCCCATCGAGAACCCCCGGGGCACCACCAGCAAGATCATGGTCCTG
  GTGTGGGCCTTCTTCGCTGTCATCTTCCTCGCTAGCTACACGGCCAATCTGGCAGCCTTCATGATCCAGG
  AACAATACATCGACACTGTGTCGGGCCTTAGTGACAAGAAGTTTCAGCGGCCTCAAGACCAATACCCACC
  CTTCCGTTTTGGCACGGTACCTAATGGCAGCACAGAGAGGAACATTCGTAGCAACTATCGTGACATGCAC
  ACTCACATGGTCAAGTTCAACCAGCGCTCGGTGGAGGATGCTCTCACAAGCCTGAAGATGGGGAAGCTGG
  ACGCCTTCATCTATGATGCCGCCGTCCTCAACTACATGGCGGGCAAGGACGAAGGCTGCAAGCTGGTCAC
  CATTGGGTCTGGCAAAGTCTTTGCCACCACTGGCTATGGCATTGCCATGCAGAAAGACTCCCACTGGAAG
  CGGGCCATAGACCTGGCGCTCCTGCAGTTCCTGGGGGATGGGGAGACACAGAAGTTGGAGACAGTGTGGC
  TCTCAGGGATCTGCCATAACGAGAAGAACGAGGTGATGAGCAGCAAGCTGGACATTGACAACATGGCGGG
  CGTCTTCTACATGCTGTTGGTGGCCATGGGGCTGGCCCTTCTGGTCTTTGCCTGGGAGCACCTGGTCTAC
  TGGAAACTTCGACACTCAGTGCCCAGCTCATCCCAGCTGGACTTCCTGCTGGCTTTCAGCAGGGGCATCT
  ACAGCTGCTTCAACGGGGTACAGAGCCTTCCGAGCCCTGCGCGGCCGCCCAGCCCGGACCTTACAGCAGG
  CTCAGCCCAGGCCAATGTGCTGAAGATGCTGCAGGCGGCTCGAGACATGGTGAGCACAGCGGACGTGAGC
  GGCTCTTTGGACCGCGCCACTCGTACCATCGAGAACTGGGGCAACAATCGCCGCGCGCCTGCTCCCACCA
  CCTCCGGCCCGCGGTCATGCACCCCGGGTCCTCCGGGACAACCGAGTCCCAGCGGCTGGCGGCCTCCCGG
  TGGGGGCCGCACCCCGCTAGCGCGCCGGGCCCCGCAGCCTCCCGCTCGCCCCGCGACCTGCGCAGGGTCG
  CCTCAGCCCGACGTGTCCCGAGCATCCTGCAGGCACGCTTGGGATGCGCGGTGGCCAGTGCGAGTCGGGC
  ATCAGGGATCGCACCTCTCGGCCTCCGAGCGGCGCGCGCTCCCGGAGCGCTCCCTGTTGCACGCGCACTG
  CCACTACAGTTCCTTCCCTCGAGCAGAGAGGTCAGGGCGCCCATTTCTCCCGCTATTCCCGGAGCCCCCG
  GAGCCCGACGACCTGCCGCTGCTCGGGCCGGAACAGCTGGCTCGGCGGGAGGCTCTGCTGCGCGCGGCCT
  GGGCCAGGGGCCCGCGCCCTCGGCACGCTTCCCTGCCCAGCTCCGTGGCAGAAGCCTTCACTCGATCCAA
  CCCTCTGCCTGCCAGGTGTACCGGTCACGCCTGCGCTTGCCCATGTCCCCAAAGCCGGCCATCCTGCCGG
  CACGTGGCTCAAACACAGTCGTTGCGGCTGCCATCCTACCGGGAGGCCTGTGTGGAGGGCGTGCCAGCAG
  GGGTGGCCGCCACCTGGCAGCCCAGACAGCATGTCTGCCTGCACACCCATACCCACCTGCCGTTCTGCTG
  GGGGACTGTCTGCCGTCACCCTCCACCCTGTTCCAGCCACAGTCCCTGGCTCATTGGAACTTGGGAGCCT
  CCATCACACAGAGGCAGGACCCTGGGGCTAGGTACAGGCTACAGGGACAGTGGGGTGCTAGAAGAGGTCA
  GCAGGGAAGCTTGTGGGACACAAGGGTTTCCAAGGTCCTGCACCTGGAGGCGGATCTCCAGCCTGGAATC
  AGAAGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010350
• Insert Size: 3720 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010350.2, NP_034480.2
• RefSeq Size: 4895 bp
• RefSeq ORF: 3720 bp
• Locus ID: 14813
• UniProt ID: Q01098
• Cytogenetics: 11 80.8 cM
• Gene Summary: Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed:1377365). Sensitivity to glutamate and channel kinetics depend on the subunit composition (PubMed:1377365). Plays a role in regulating the balance between excitatory and inhibitory activity of pyramidal neurons in the prefrontal cortex (PubMed:27922130). Contributes to the slow phase of excitatory postsynaptic current, long-term synaptic potentiation, and learning (PubMed:8987814).[UniProtKB/Swiss-Prot Function]