Ercc6l (NM_146235) Mouse Untagged Clone

CAT#: MC224026

Ercc6l (untagged) - Mouse excision repair cross-complementing rodent repair deficiency complementation group 6 - like (Ercc6l), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ercc6l
• Synonyms: BC004701; D330021P09Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224026 representing NM_146235
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCTTCCCAAGGCTTAGCGGAAGTGGAGACTTTGAGCCCACAACTGGCCGAGAGTTACCTGAGAT
  ATGTGCAAGAAGCCAAAGAAGCAGCTAAGAATGGAGATCTGGAAGAATCATTGAAACTTTTCAATTTGGC
  AAAGGACATTTTTCCCACCAAAAAGGTGATGAGCAGAATCCAGAAACTCCAGGAAGCTTTGGAGCAGTTG
  GCAGAAGAAGAAGATGATGATGAATTTATAGATGTGTGCAGCTCTGGCTTGCTGCTTTACCGAGAGCTGT
  ATGAGAAACTCTTTGAGCACCAGAAAGAAGGCATCGCTTTTCTCTATAGCCTGTATAAGGATGGAAGAAA
  AGGGGGCATCTTAGCAGATGATATGGGACTGGGGAAGACTGTTCAGATCATTGCTTTCCTGTCAGGTATG
  TTTGATGCTTCACTTGTGAATCATGTGCTATTGATCATGCCAACCAATCTCATTAATACATGGGTCAACG
  AATTTGCCAAGTGGACTCCAGGAATGAGAGTGAAAACCTTTCATGGCTCCAGCAAGAGTGAACGCACTAG
  AAGCCTGACACGGATTCAACAAAGGAACGGCGTTGTGATCACTACCTACCAAATGTTACTCAATAATTGG
  CAGCAACTTGCAAGCTTTAATGGACAAGCATTTGTATGGGACTACGTCATCCTTGATGAAGCCCATAAGA
  TCAAAAGCGCCTCTACCAAGTCAGCAGTGTGTGCACGTGCTATTCCTGCAAGTAATCGCCTCCTCCTCAC
  AGGTACACCAGTCCAGAATAACCTGCAAGAACTCTGGTCCCTGTTTGATTTTGCTTGCCAAGGCTCCTTG
  TTAGGAACATTAAAAACTTTTAAAATGGAGTATGAGCATCCGATTATTAGAGCAAGAGAGAAGGATGCCA
  CCCCTGGGGAAAAAGCCTTGGGACTTAAGATATCTGAAAATTTAATGGAAATCATAAAACCCTATTTTCT
  AAGGAGGACCAAAGAAGAAGTGCAGACGAAAAAGGCAGACAATCCAGAGGCCAGACTTGGTGAAAAGAAT
  CCAGCTGGTGAAGCCATTTGTGACATGTTTTCCCTTGCCAGGAAAAATGATTTAATTGTTTGGATACGCC
  TTCTGCCTTTACAAGAGGAAATTTATAGGAAGTTTGTGTCTTTAGATCACATCAAGGAGTTGTTAATGGA
  GACACGCTCACCTCTAGCTGAGCTGGGTGTCTTAAAGAAGCTCTGTGACCACCCTAGGCTGCTGTCTGCA
  CGAGCTTGCCGTTTGCTGAATCTAGGAACTGCCACATTCTCTGCTCAGGATGAAAACGAGCAAGAAGATG
  TCTCAAATATGAACAGCATTGATCACTTACCTGACAAAACCCTGATACAAGAGTCTGGAAAAATGATATT
  CTTAATGTCCCTGCTAGAAAGACTTCAAGATGAAGGACATCAAACTCTGGTGTTTTCTCAGTCGATAAAA
  ATTCTCAACATCATCGAGCGTCTCTTAAAGAACAAGCACTTTAAGACATTGCGAATTGATGGCACAGTTA
  CTCATCTTTGGGAACGAGAAAAAAGAATTCAGTTATTCCAGCAAAATAAAGAATACTCAGTTTTTCTACT
  TACCACTCAAGTAGGTGGTGTTGGCCTAACATTAACTGCAGCGACTAGAGTTGTCATTTTTGACCCTAGC
  TGGAACCCTGCAACTGATGCTCAGGCTGTGGATAGAGTTTACCGAATTGGCCAAAAAGAGAATGTTGTGG
  TTTATCGACTAATCACTTGTGGAACTGTAGAAGAAAAGATATATAGAAGACAGGTTTTCAAGGACTCCTT
  GATCAGACAAACTACTGGTGAGAAGAAGAACCCATTCCGCTATTTCACCAAACAAGAGTTAAAAGAGCTC
  TTCACAGTTGGGGACCTGCAGAAGTCTGCGACCCAGATGCAGCTTCAGTGTCTACATGCTGCTCAGCGGA
  GATCTGATGAGAAACTAGATGAGCATATTGCCTACCTCCACTTGCTGGGCATAGCAGGAATCTCAGACCA
  TGATTTGATGTTCACCCGTGATCTGTCTGTTAAAGAAGAGCTTGACATGCTAGAAGACTCTCAGTACATT
  CATCAGAGAGTTCAAAAAGCTCAATTCCTTGTTGAATCTGAGTCTCAAAATACAGTGCAAAGACAAACGA
  CTGGAATTGAGGAGACCTGGCTCAAAGCACAGGAATTTCCTTCTCAACAGAAGAAGAAAGGTACTGAATT
  CAACAAACCTCAGCCCCAGCCTTCACGCCTTCTGACTAAACCTACCCAGGTAGAGGCTATCAGTTCTCAA
  ATGGCCAGTATAACCATTTGCGATCAGTCTGCAGAGAGTGAGCCCCAAGAGCACTCTGAGGTACATGATG
  TCACTTCACTGCAAGGTAGTCACCACTTCAACAGTACATCTGATGCTGGCACTATAGCTTCTTTACCCCA
  GGGGGCTGAAAGTATAGGCGAAGTTTCTACTGACTCTTTATTGAGCCCTGCAAAAGGTTTTGCAGCTGAA
  AATGATGCTATGCAGAAGAAGGGATTACAAGCATCACCTGGGCAAGAGGCCCCATCAGAGAACCTGGGAA
  GTTTTCATTATTTACCTAGAGAATCCAGCAAGGCCAGTCTTGGGCCAAATTTAGATCTACAGGATAGTGT
  GGTTTTATATCACCGCAGTCCCACAGCAAATGAAAACCAAAACCTAGAATCAGATGTACCCATGATTGAA
  ATATCTGATGACTTGTCTGAACCTCCTAGCGCACTGCAGGGTGCTCAAGCAATCGAGGCCCAGTTGGAGT
  TGAAAGAGGATGACCCTTTAAAATCACCACCACAATATGCATGTGATTTCAATCTTTTCCTGGAAGACTC
  TGCAGACACTAGACAAAACCTCTCCAGCAAGTTTTTGGAACATGTTGAGAAAGAAAAAAGCTTGCAAAGC
  CCTGCAGCTAACTCCAGGGCAAAGTCAGCACTTACTCTCAGTTTGGATTCTTCTCCTAAGAGTGATGAAG
  AATCAGAAGTGATTAGTGTGAAAACCAAAAGCAAAACTAGAAGGATCCTCTCAGATGATGAAGATGAAGA
  TGAAGAAGATGCTTTTAAAGGTAGTCATACAAATTCAATTAACATCTCTCCCTTTCCATTCTCATCTGTG
  AAACAATTTGATGCTTCAACTCCCCAAAGTGGCAGCAATCCATCTCGAAGGTTCTTTTCACCTAAAACAC
  CAGGTGAAGTGAATACGTCCTTACATTCTAGGAGGTCCCTGGCTTCTAGGAGGTCTCTGATCAATGTGGT
  TTTAGATGATGTGGAAGATATGGAGGAAAGGCTTGATAACAGCAGCGAGGAGGAGAGTGAGCCAGGGCTT
  TCAGAAGAGAACAATGAAGAAGAAGCCTTAGCCTGCACAGAAGAGCAGCCTTCTGGGGCAACACTGGCTT
  CAGGAAACAAGTCCAGCAACTTGACCATGTCTGAGCCTACTTCTCCAGCCCCACAAAGCTCTCCCTGTGC
  TCCTGAACCTTCGTCAAGTGACCCTATGCCTGATCCTCCCCAGGATCTGGCAGTGGAGGCTGGTAATGAC
  TATGAGAGTCTTGTAGCTCGTGGGAAAGAACTGAAAGAGTGCGGGAAGATACAGGAGGCTCTCAACTGCT
  TAGTTAAAGCACTTGATATAAAAAGTGCAGATCCTGAGGTCATGCTCATGACTTTAAGTTTGTATAAGCA
  GCTGAACATTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_146235
• Insert Size: 3723 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146235.3, NP_666347.2
• RefSeq Size: 4198 bp
• RefSeq ORF: 3723 bp
• Locus ID: 236930
• UniProt ID: Q8BHK9
• Cytogenetics: X D
• Gene Summary: DNA helicase that acts as an essential component of the spindle assembly checkpoint. Contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores and monitoring tension on centromeric chromatin. Acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase. Functions as ATP-dependent DNA translocase. Can promote Holliday junction branch migration (in vitro).[UniProtKB/Swiss-Prot Function]