Gnptab (NM_001004164) Mouse Untagged Clone

CAT#: MC224020

Gnptab (untagged) - Mouse N-acetylglucosamine-1-phosphate transferase, alpha and beta subunits (Gnptab), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gnptab
• Synonyms: EG432486; mKIAA1208
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224020 representing NM_001004164
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTTAAGCTCCTGCAGAGACAGACCTATACCTGCCTATCCCACAGGTATGGGCTCTACGTCTGCT
  TCGTGGGCGTCGTTGTCACCATCGTCTCGGCTTTCCAGTTCGGAGAGGTGGTTCTGGAATGGAGCCGAGA
  TCAGTACCATGTTTTGTTTGATTCCTACAGAGACAACATTGCTGGGAAATCCTTTCAGAATCGGCTCTGT
  CTGCCCATGCCAATCGACGTGGTTTACACCTGGGTGAATGGCACTGACCTTGAACTGCTAAAGGAGCTAC
  AGCAGGTCCGAGAGCACATGGAGGAAGAGCAGAGAGCCATGCGGGAAACCCTCGGGAAGAACACAACCGA
  ACCGACAAAGAAGAGTGAGAAGCAGCTGGAATGTCTGCTGACGCACTGCATTAAGGTGCCCATGCTTGTT
  CTGGACCCGCCCCTGCCAGCCAACTGCACCCTGAAGGATCTGCCAACCCTTTATCCATCTTTCCACGCTG
  CCAGCGACATGTTCAATGTTGCGAAACCAAAAAATCCGTCTACTAATGTCTCCGTTGTCGTTTTTGACAC
  TACTAAGGATGTTGAAGACGCCCATGCTGGACCGTTTAAGGGAGGCAGCAAACAGATGGTTTGGAGAGCC
  TACTTGACAACAGACAAAGAAGCCCCTGGCTTAGTGCTGATGCAAGGCTTGGCGTTCCTGAGTGGATTCC
  CACCGACCTTCAAGGAGACGAGTCAACTGAAGACAAAGCTGCCAGAAAAGCTTTCCTCTAAAATAAAGCT
  GTTGCGGCTGTACTCGGAGGCCAGTGTCGCTCTTCTGAAATTGAATAATCCCAAGGGTTTCCAAGAGCTG
  AACAAGCAGACCAAGAAGAACATGACCATCGATGGGAAGGAACTGACCATCAGCCCTGCGTATCTGCTGT
  GGGACCTGAGTGCCATCAGCCAGTCCAAGCAGGATGAGGACGTGTCTGCCAGCCGCTTTGAGGATAATGA
  AGAGCTGAGGTACTCGCTGCGATCTATCGAGAGACACGCGCCATGGGTACGGAATATTTTCATTGTCACC
  AACGGGCAGATTCCATCCTGGCTGAACCTTGACAACCCTCGAGTGACCATAGTGACCCACCAGGACATTT
  TCCAAAATCTGAGCCACTTGCCTACTTTCAGTTCCCCTGCTATTGAAAGTCACATTCACCGCATCGAAGG
  GCTGTCCCAGAAGTTTATTTATCTAAATGACGATGTCATGTTCGGTAAGGACGTCTGGCCGGACGATTTT
  TACAGCCACTCCAAAGGTCAAAAGGTTTATTTGACATGGCCTGTGCCAAACTGTGCAGAGGGCTGCCCGG
  GCTCCTGGATTAAAGACGGCTATTGTGATAAGGCCTGTAATAACTCAGCCTGTGACTGGGATGGCGGAGA
  CTGCTCTGGTAATACTGCAGGGAACCGGTTTGTTGCAGGAGGTGGGGGTACCGGGAATATTGGAGCTGGA
  CAGCACTGGCAGTTTGGTGGAGGAATAAACACCATCTCTTACTGTAACCAAGGATGTGCAAACTCCTGGC
  TGGCTGACAAGTTCTGTGACCAAGCCTGTAACGTCTTATCCTGCGGGTTTGATGCTGGTGACTGTGGACA
  AGATCATTTTCATGAATTGTATAAAGTAACACTTCTCCCAAACCAGACTCACTATGTTGTCCCCAAAGGT
  GAATACCTGTCTTATTTCAGCTTTGCAAACATAGCCAGAAGAGGAGTTGAAGGGACCTACAGCGACAACC
  CCATCATCCGCCACGCGTCCATTGCAAACAAGTGGAAAACCATACACCTGATAATGCACAGTGGGATGAA
  CGCAACCACGATCTATTTTAACCTCACTCTTCAAAACGCCAACGACGAAGAGTTCAAGATCCAGATAGCA
  GTAGAGGTGGACACGAGGGAGGCGCCCAAACTGAATTCTACAACCCAGAAGGCCTATGAAAGTTTGGTTA
  GCCCAGTGACACCTCTTCCTCAGGCTGACGTCCCTTTTGAAGATGTCCCCAAAGAGAAACGCTTCCCCAA
  GATCAGGAGACATGATGTAAATGCAACAGGGAGATTCCAAGAGGAGGTGAAAATCCCCCGGGTAAATATT
  TCACTCCTTCCCAAAGAGGCCCAGGTGAGGCTGAGCAACTTGGATTTGCAACTAGAACGTGGAGACATCA
  CTCTGAAAGGATATAACTTGTCCAAGTCAGCCCTGCTAAGGTCTTTCCTGGGGAATTCACTAGATACTAA
  AATAAAACCTCAAGCTAGGACCGATGAAACAAAAGGCAACCTGGAGGTCCCACAGGAAAACCCTTCTCAC
  AGACGTCCACATGGCTTTGCTGGTGAACACAGATCAGAGAGATGGACTGCCCCAGCAGAGACAGTGACCG
  TGAAAGGCCGTGACCACGCTTTGAATCCACCCCCGGTGTTGGAGACCAATGCAAGATTGGCCCAGCCTAC
  ACTAGGCGTGACTGTGTCCAAAGAGAACCTTTCACCGCTGATCGTTCCCCCAGAAAGCCACTTGCCAAAA
  GAAGAGGAGAGTGACAGGGCAGAAGGCAATGCTGTACCTGTAAAGGAGTTAGTGCCTGGCAGACGGTTGC
  AGCAGAATTATCCAGGCTTTTTGCCCTGGGAGAAAAAAAAGTATTTCCAAGACCTTCTTGATGAGGAAGA
  GTCATTGAAGACCCAGTTGGCGTACTTTACAGACAGCAAACATACCGGGAGGCAACTAAAAGATACATTT
  GCAGACTCCCTCCGATACGTCAATAAAATTCTCAACAGCAAGTTTGGATTCACATCCAGGAAAGTCCCTG
  CACACATGCCGCACATGATTGACAGGATCGTTATGCAAGAACTCCAAGATATGTTCCCTGAAGAATTTGA
  CAAGACTTCATTTCACAAGGTGCGTCACTCTGAGGACATGCAGTTTGCCTTCTCCTACTTTTATTACCTC
  ATGAGTGCAGTTCAGCCCCTCAATATTTCCCAAGTCTTTCATGAAGTAGACACAGACCAATCTGGTGTCT
  TGTCTGATAGGGAAATCCGAACACTGGCCACGAGAATTCACGACCTACCTTTAAGCTTGCAGGATTTGAC
  AGGTTTGGAACACATGTTAATAAATTGCTCAAAAATGCTCCCCGCTAATATCACTCAACTCAACAACATC
  CCACCGACTCAGGAAGCATACTACGACCCCAACCTGCCTCCGGTCACTAAGAGTCTTGTCACCAACTGTA
  AGCCAGTAACTGACAAGATCCACAAAGCCTATAAAGACAAGAACAAATACAGGTTTGAAATCATGGGAGA
  GGAAGAAATCGCTTTCAAGATGATACGAACCAATGTTTCTCATGTGGTTGGCCAGTTGGATGACATCAGA
  AAAAACCCCAGGAAGTTCGTTTGTCTGAATGACAACATTGACCACAACCATAAAGATGCCCGGACAGTGA
  AGGCTGTCCTCAGGGACTTCTATGAGTCCATGTTTCCCATACCTTCCCAGTTTGAGCTGCCAAGAGAGTA
  TCGGAACCGCTTTCTGCACATGCATGAGCTCCAAGAATGGCGGGCATATCGAGACAAGCTGAAGTTTTGG
  ACCCACTGCGTACTAGCAACGTTGATTATATTTACTATATTCTCATTTTTTGCTGAACAGATAATTGCTC
  TGAAGCGAAAGATATTTCCCAGGAGGAGGATACACAAAGAAGCTAGTCCAGACCGAATCAGGGTGTAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001004164
• Insert Size: 3708 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004164.2, NP_001004164.2
• RefSeq Size: 5204 bp
• RefSeq ORF: 3708 bp
• Locus ID: 432486
• UniProt ID: Q69ZN6
• Cytogenetics: 10 C1
• Gene Summary: Catalyzes the formation of mannose 6-phosphate (M6P) markers on high mannose type oligosaccharides in the Golgi apparatus. M6P residues are required to bind to the M6P receptors (MPR), which mediate the vesicular transport of lysosomal enzymes to the endosomal/prelysosomal compartment.[UniProtKB/Swiss-Prot Function]