Cand1 (NM_027994) Mouse Untagged Clone

CAT#: MC224016

Cand1 (untagged) - Mouse cullin associated and neddylation disassociated 1 (Cand1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cand1
• Synonyms: 2310038O07Rik; 6330512O03Rik; AI195005; AI846556; D10Ertd516e; mKIAA0829; Tp120a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224016 representing NM_027994
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAGCGCTTCGTACCACATCTCCAACTTGCTGGAGAAAATGACATCCAGCGACAAGGACTTCAGGT
  TCATGGCGACCAACGACCTGATGACAGAGCTGCAGAAAGACTCTATCAAGCTGGACGATGACAGCGAGAG
  GAAAGTTGTGAAGATGATTCTCAGGTTGCTGGAGGATAAAAATGGCGAAGTGCAGAACTTAGCTGTCAAA
  TGCCTTGGCCCTTTGGTGAGTAAAGTGAAAGAGTACCAAGTGGAGACGATCGTGGACACCCTCTGCACCA
  ACATGCTTTCTGACAAGGAGCAGCTGCGAGACATTTCCAGCATCGGCCTGAAAACAGTAATTGGAGAACT
  TCCGCCAGCTTCCAGTGGCTCTGCATTAGCTGCTAATGTATGTAAAAAGATTACTGGACGCCTTACCAGT
  GCAATAGCAAAGCAGGAAGATGTTTCTGTTCAGCTCGAAGCCTTGGATATCATGGCTGACATGTTGAGCA
  GGCAAGGAGGACTGCTTGTTAATTTCCACCCTTCAATTCTGACCTGTCTGCTTCCCCAGCTGACCAGCCC
  TAGACTTGCAGTGAGGAAAAGAACAATTATTGCTCTTGGCCACCTAGTTATGAGCTGCGGAAATATAGTT
  TTTGTAGACCTTATTGAACATCTGTTGTCAGAGTTGTCCAAAAATGACTCCATGTCAACAACAAGGACCT
  ACATACAGTGTATTGCTGCTATTAGTAGGCAAGCTGGCCATAGAATAGGTGAATACCTTGAAAAGATAAT
  TCCTTTGGTGGTAAAATTTTGTAATGTAGATGATGATGAATTAAGAGAATACTGCATTCAAGCTTTTGAA
  TCATTTGTGAGAAGATGCCCTAAAGAAGTTTATCCTCATGTTTCTACCATTATAAACATTTGTCTAAAAT
  ACCTTACCTATGATCCAAATTACAATTACGATGATGAAGATGAAGATGAGAATGCTATGGATGCTGACGG
  TGGGGACGACGATGACCAAGGGAGTGACGATGAATATAGTGACGATGACGACATGAGTTGGAAAGTGAGA
  CGCGCGGCTGCTAAGTGCCTGGACGCTGTAGTTAGCACACGGCATGAGATGCTCCCGGAGTTCTACAAGA
  CTGTCTCTCCTGCGCTGATAGCCAGATTTAAAGAGCGGGAAGAAAATGTAAAGGCAGATGTTTTTCATGC
  ATACCTGTCTCTTCTGAAACAAACTCGTCCAGTGCAAAGTTGGCTGTGTGACCCTGACGCAATGGAGCAG
  GGAGACACGCCCCTAACAATGCTGCAGAGTCAGGTTCCCAACATTGTTAAAGCCCTGCACAAGCAGATGA
  AAGAGAAGAGTGTGAAGACCCGACAGTGTTGCTTTAACATGTTAACTGAGCTGGTAAATGTGCTGCCGGG
  AGCACTAACCCAGCACATTCCTGTACTGGTACCAGGAATCATTTTCTCACTGAATGATAAGTCCAGCTCA
  TCAAACCTGAAGATTGATGCGCTGTCGTGTCTGTATGTCATCCTCTGTAACCACTCTCCTCAAGTTTTCC
  ATCCTCATGTTCAGGCTCTGGTCCCTCCGGTGGTGGCCTGTGTGGGAGACCCGTTTTACAAGATCACTTC
  AGAAGCCCTTCTTGTCACTCAGCAGCTTGTCAAAGTGATCCGTCCTCTAGACCAGCCCTCTTCGTTCGAT
  GCAACGCCTTACATCAAAGATCTCTTCACCTGCACAATTAAGCGCTTAAAAGCAGCTGACATTGATCAAG
  AAGTCAAGGAAAGGGCTATTTCCTGTATGGGACAGATTATTTGCAATCTTGGAGACAATTTGGGTCCTGA
  TTTATCAAATACCCTACAGATTTTCTTGGAGAGACTCAAGAATGAGATTACCCGGCTAACGACCGTCAAA
  GCGCTGACCCTGATTGCTGGGTCACCTTTGAAGATAGATCTGAGGCCTGTGCTGGGAGAGGGAGTCCCCA
  TCCTTGCTTCATTCCTCAGGAAAAATCAGAGAGCTTTGAAACTGGGGACCCTCTCTGCCCTGGATATTCT
  CATTAAGAACTACAGTGACAGCCTGACGGCCGCCATGATTGACGCAGTTCTGGATGAGCTCCCCCCCCTC
  ATCAGCGAGAGCGATATGCACGTGTCGCAGATGGCTATCAGCTTCCTCACCACCCTGGCCAAGGTCTATC
  CCTCCTCCCTGTCCAAGATAAGCGGATCTATCCTCAATGAACTGATTGGACTTGTGAGATCGCCTCTACT
  GCAGGGAGGAGCGCTCAGTGCCATGCTAGACTTTTTCCAAGCTCTGGTTGTCACTGGAACAAACAATCTA
  GGGTACATGGACTTGCTGCGCATGCTAACGGGTCCAGTTTATTCTCAGAGCACTGCTCTCACTCATAAGC
  AGTCTTATTATTCCATTGCCAAATGTGTAGCTGCCCTTACTCGAGCTTGCCCTAAAGAGGGACCCGCCGT
  AGTAGGTCAGTTTATTCAAGATGTCAAGAACTCAAGGTCTACAGATTCCATTCGTCTCTTAGCACTCCTT
  TCTCTTGGAGAAGTTGGACACCATATTGACTTAAGTGGGCAGTTGGAATTAAAATCTGTAATATTAGAGG
  CTTTCTCATCCCCTAGTGAAGAAGTCAAATCAGCTGCGTCCTATGCATTAGGCAGCATTAGTGTAGGCAA
  CCTTCCTGAGTATCTGCCCTTTGTACTGCAAGAAATAACCAGCCAGCCCAAAAGGCAGTATCTTCTGCTT
  CACTCTTTGAAGGAGATCATTAGCTCTGCATCGGTGGCAGGCCTTAAGCCATATGTTGAGAACATCTGGG
  CCTTGCTGCTAAAGCACTGTGAGTGTGCGGAGGAAGGCACCAGGAATGTCGTGGCTGAATGTCTAGGGAA
  GCTCACTCTTATTGATCCTGAAACTCTCCTCCCACGGCTTAAAGGGTATTTGATATCAGGGTCATCATAT
  GCCAGGAGCTCGGTGGTTACAGCTGTGAAGTTCACGATTTCTGATCATCCTCAGCCTATTGATCCACTGT
  TGAAGAACTGCATAGGTGACTTTCTAAAAACTTTGGAAGACCCAGATTTGAACGTAAGAAGAGTGGCCTT
  GGTCACATTCAATTCTGCGGCCCATAACAAGCCATCACTGATACGGGACCTTCTGGACTCAGTTCTTCCA
  CATCTTTACAATGAGACAAAAGTTAGGAAGGAACTTATAAGAGAGGTAGAAATGGGTCCATTTAAACACA
  CGGTTGATGACGGTCTAGACATTAGAAAGGCAGCTTTTGAGTGTATGTATACACTTCTAGACAGCTGTCT
  TGATAGACTGGATATCTTTGAATTTCTAAATCATGTTGAAGATGGTTTGAAGGACCATTATGATATTAAG
  ATGCTAACATTTTTAATGTTGGTACGACTGTCTACCCTCTGTCCAAGTGCAGTACTACAAAGGCTGGACC
  GACTTGTTGAGCCACTGCGTGCTACGTGTACAACTAAGGTAAAGGCAAACTCTGTAAAGCAGGAGTTTGA
  AAAGCAAGATGAGCTAAAGCGGTCTGCCATGAGGGCAGTGGCGGCTTTGTTAACCATTCCAGAGGCAGAG
  AAGAGCCCTCTTATGAGTGAATTCCAGTCACAGATCAGCTCCAACCCGGAGCTGGCAGCCATCTTTGAAA
  GTATCCAGAAAGATTCTTCGTCCACCAACTTGGAATCAATGGACACGAGTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027994
• Insert Size: 3693 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027994.1, NP_082270.1
• RefSeq Size: 8209 bp
• RefSeq ORF: 3693 bp
• Locus ID: 71902
• UniProt ID: Q6ZQ38
• Cytogenetics: 10 67.08 cM
• Gene Summary: Key assembly factor of SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes that promotes the exchange of the substrate-recognition F-box subunit in SCF complexes, thereby playing a key role in the cellular repertoire of SCF complexes. Acts as a F-box protein exchange factor. The exchange activity of CAND1 is coupled with cycles of neddylation conjugation: in the deneddylated state, cullin-binding CAND1 binds CUL1-RBX1, increasing dissociation of the SCF complex and promoting exchange of the F-box protein. Probably plays a similar role in other cullin-RING E3 ubiquitin ligase complexes (By similarity).[UniProtKB/Swiss-Prot Function]