Irs1 (NM_010570) Mouse Untagged Clone

CAT#: MC224006

Irs1 (untagged) - Mouse insulin receptor substrate 1 (Irs1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Irs1
• Synonyms: G972R; IRS-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224006 representing NM_010570
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAGCCCTCCGGATACCGATGGCTTCTCAGACGTGCGCAAGGTGGGCTACCTGCGCAAGCCCAAGA
  GTATGCATAAGCGCTTTTTCGTGCTGCGGGCGGCCAGCGAGGCCGGGGGCCCAGCGCGCCTGGAGTATTA
  TGAGAACGAGAAGAAGTGGCGGCACAAGTCGAGCGCCCCCAAACGCTCGATCCCCCTCGAGAGCTGTTTC
  AACATCAACAAGCGGGCTGACTCCAAGAACAAGCACCTGGTGGCTCTCTACACCCGAGACGAACACTTTG
  CCATTGCCGCGGATAGCGAGGCTGAGCAAGACAGCTGGTACCAGGCTCTTCTGCAGCTGCATAATCGGGC
  AAAGGCCCACCATGACGGGGCTGGAGGAGGCTGCGGCGGTAGCTGCAGCGGCAGCTCTGGCGTTGGAGAG
  GCAGGGGAGGACTTGAGCTATGACACGGGTCCAGGACCCGCGTTCAAGGAGGTCTGGCAGGTTATCCTGA
  AACCCAAGGGCCTGGGTCAGACAAAGAACCTGATTGGCATCTACCGCCTCTGCCTGACCAGCAAGACCAT
  CAGCTTTGTGAAGCTGAACTCCGAGGCAGCCGCTGTGGTGCTGCAGCTGATGAATATCAGACGCTGTGGC
  CACTCAGAGAACTTCTTCTTCATCGAGGTGGGGCGTTCCGCTGTGACAGGGCCTGGCGAGTTCTGGATGC
  AAGTGGATGACTCCGTGGTGGCCCAGAACATGCATGAGACCATTCTAGAGGCAATGAGGGCCATGAGCGA
  TGAGTTTCGCCCTCGCAGCAAAAGCCAGTCTTCATCCAGTTGCTCCAACCCCATCAGCGTTCCCCTGCGC
  AGGCACCATCTCAACAACCCTCCACCCAGCCAGGTGGGACTGACTCGGAGATCTCGAACTGAGAGCATCA
  CTGCCACCTCCCCTGCCAGTATGGTGGGTGGGAAACCAGGTTCCTTCCGGGTGCGTGCCTCCAGCGATGG
  CGAAGGCACCATGTCCCGTCCAGCATCAGTGGATGGCAGTCCTGTGAGCCCTAGCACCAACAGGACCCAC
  GCCCATCGGCATCGAGGCAGCTCCAGGCTGCACCCCCCACTCAACCACAGCCGCTCCATCCCCATGCCTT
  CTTCTAGATGCTCACCTTCAGCCACCAGCCCAGTGAGTCTGTCATCTAGTAGTACCAGTGGCCATGGCTC
  CACTTCAGACTGTCTCTTCCCGAGGCGCTCTAGTGCTTCCGTGTCCGGTTCGCCTAGCGATGGCGGTTTC
  ATCTCTTCTGATGAGTATGGTTCTAGTCCCTGCGATTTCCGAAGTTCCTTCCGCAGTGTCACCCCAGATT
  CCCTGGGGCACACCCCACCAGCCAGGGGTGAGGAGGAGCTGAGCAATTATATCTGCATGGGTGGCAAGGG
  AGCCTCCACCTTGGCTGCTCCCAATGGCCACTACATTTTGTCTAGGGGTGGCAACGGCCATCGCTACATC
  CCAGGTGCTAACTTGGGGACAAGCCCAGCGCTGCCTGGAGATGAAGCCGCGGGTGCAGCAGATCTGGATA
  ACCGGTTTCGAAAGAGAACTCACTCCGCAGGCACATCTCCTACCATTTCCCATCAGAAGACCCCCTCACA
  GTCTTCAGTGGCTTCTATTGAGGAATATACAGAGATGATGCCCGCTGCCTACCCACCAGGAGGTGGCAGT
  GGAGGCCGACTGCCCGGCTACCGGCATTCCGCCTTCGTGCCCACCCACTCCTATCCCGAAGAGGGTCTAG
  AGATGCACCACTTGGAACGTCGTGGAGGCCACCACCGTCCAGACACCTCCAACCTCCACACTGATGATGG
  CTATATGCCCATGTCTCCTGGGGTGGCTCCAGTGCCCAGCAACCGCAAAGGAAATGGGGACTATATGCCC
  ATGAGCCCCAAGAGTGTATCTGCCCCACAGCAGATCATTAACCCCATCAGACGCCACCCACAGAGAGTGG
  ACCCCAATGGCTACATGATGATGTCACCCAGTGGTAGTTGCTCCCCTGACATTGGAGGTGGGTCCAGCAG
  CAGTAGCAGCATCAGCGCAGCCCCTTCTGGGAGCAGCTATGGGAAGCCATGGACAAATGGAGTAGGGGGG
  CACCATACTCATGCCCTTCCTCATGCCAAACCTCCTGTTGAGAGTGGTGGCGGTAAGCTCTTGCCTTGCA
  CAGGTGACTACATGAACATGTCCCCAGTGGGAGATTCCAACACCAGCAGCCCCTCAGAATGCTACTATGG
  CCCAGAAGATCCCCAGCACAAGCCGGTCCTCTCTTACTACTCATTGCCAAGGTCCTTTAAGCACACCCAG
  CGCCCTGGAGAGCCAGAGGAGGGTGCCAGGCACCAGCATCTTCGTCTCTCTTCTAGCTCTGGACGCCTTC
  GCTATACCGCAACTGCCGAAGATTCCTCCTCTTCTACCAGCAGCGACAGTCTGGGTGGGGGTTACTGTGG
  GGCTAGGCCAGAGTCAAGCCTCACACATCCCCACCACCATGTCTTGCAGCCCCACCTGCCTCGAAAGGTA
  GACACAGCTGCACAGACCAACAGCCGCCTGGCTCGACCCACAAGGCTGTCCTTGGGGGATCCCAAGGCAA
  GCACCTTACCCCGGGTTCGAGAGCAGCAACAGCAGCAGCAGTCTTCCCTGCACCCTCCCGAGCCCAAAAG
  CCCAGGAGAATATGTGAATATTGAATTCGGGAGCGGCCAGCCTGGCTATTTAGCTGGCCCTGCAACTTCC
  CGTAGCTCTCCTTCAGTTCGATGTCCACCCCAGCTCCACCCAGCTCCTAGAGAAGAGACTGGCTCGGAAG
  AGTACATGAACATGGACTTGGGGCCAGGCCGGAGGGCAACCTGGCAGGAGAGTGGTGGAGTTGAGTTGGG
  CAGAATAGGCCCTGCACCTCCGGGGTCTGCTACGGTTTGCAGGCCAACCCGTTCGGTGCCAAATAGCCGT
  GGTGACTACATGACCATGCAGATAGGTTGTCCTCGTCAAAGCTATGTGGATACCTCACCAGTGGCCCCAG
  TCAGCTATGCTGACATGCGGACAGGCATTGCTGCAGAGAAGGCGAGCCTGCCTAGACCCACAGGAGCTGC
  TCCTCCTCCATCCTCCACAGCCTCTTCTTCTGTTACACCTCAAGGAGCCACCGCTGAGCAAGCTACTCAC
  TCTTCCTTGCTGGGAGGCCCTCAGGGACCTGGGGGCATGAGTGCATTCACCAGGGTGAACCTCAGTCCCA
  ACCATAACCAGAGTGCCAAAGTGATTCGCGCAGACACTCAAGGGTGCCGGAGGAGGCATAGCTCTGAGAC
  CTTCTCAGCACCTACTCGGGCTGGCAATACGGTGCCCTTTGGAGCAGGGGCTGCAGTAGGGGGCAGCGGC
  GGTGGTGGTGGTGGCGGCAGTGAGGATGTAAAACGCCACAGCTCTGCATCCTTTGAGAATGTGTGGCTGA
  GACCTGGGGATCTAGGGGGAGTCTCCAAGGAGTCGGCTCCAGTGTGTGGGGCTGCTGGGGGTTTGGAGAA
  GAGTCTTAACTACATAGACCTGGATTTGGCCAAGGAGCGCTCTCAGGACTGCCCCTCTCAACAGCAGTCC
  CTACCACCCCCTCCCCCTCACCAGCCCTTAGGCAGCAATGAGGGCAACTCCCCAAGACGCTCCAGTGAGG
  ATTTAAGCAACTATGCCAGCATCAGCTTCCAGAAGCAGCCAGAGGATCGTCAATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010570
• Insert Size: 3696 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010570.4, NP_034700.2
• RefSeq Size: 9163 bp
• RefSeq ORF: 3696 bp
• Locus ID: 16367
• UniProt ID: P35569
• Cytogenetics: 1 42.0 cM
• Gene Summary: May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).[UniProtKB/Swiss-Prot Function]