Arid4b (NM_198122) Mouse Untagged Clone

CAT#: MC223999

Arid4b (untagged) - Mouse AT rich interactive domain 4B (RBP1-like) (Arid4b), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arid4b
• Synonyms: BCAA; BRCAA1; RBBP1L1; Rbp1l1; SAP180
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223999 representing NM_198122
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGCCCTTGATGAACCTCCTTATTTGACAGTGGGCACTGATGTGAGTGCCAAATACAGAGGAGCCT
  TCTGTGAAGCCAAGATCAAGACTGCAAAAAGACTTGTCAAAGTCAAGGTGACTTTTAGACACGATTCTTC
  AACAGTGGAAGTTCAGGATGACCACATAAAGGGCCCACTGAAGGTAGGAGCTATTGTGGAAGTGAAGAAT
  CTTGATGGTGCCTATCAGGAAGCGGTTATCAATAAACTGACAGATGCAAGTTGGTATACTGTGGTTTTTG
  ATGATGGAGATGAGAAGACACTGAGAAGATCTTCATTATGTCTGAAAGGAGAGAGACATTTTGCTGAAAG
  TGAAACACTAGACCAGCTCCCACTCACCAATCCTGAGCATTTTGGCACTCCAGTCATAGGAAAGAAAACA
  AATAGAGGAAGAAGATCTAACCATATACCAGAGGAGGAGTCTTCCTCCTCATCCAGTGATGACGATGAGG
  AGGAGAGGAAACAGACTGATGAGCTACTAGGCAAAGTTGTTTGTGTAGATTATGTTAGCTTGGAGAAAAA
  GAAAGCGATGTGGTTTCCTGCACTGGTGGTTTGTCCTGACTGTAGTGATGAGATTGCTGTGAAAAAAGAC
  AATATTCTTGTTCGGTCTTTCAAAGATGGCAAATTTACTTCAGTTCCAAGAAAAGATGTCCATGAAATTA
  CTAGTGACACTGTACCGAAGCCTGATGCTGTATTAAAACAAGCCTTTGATCAGGCACTTGAGTTTCACAA
  AAGTAGAGCTATTCCTGCTAATTGGAAGACTGAATTGAAAGAAGACAGCTCCAGTAGTGAAGCTGAGGAG
  GAGGAGGAGGAGGAAGATGATGAAAAAGAGAAAGAAGATAACAGCAGCGAAGAAGAGGAAGAAATAGAAC
  CATTTCCAGAGGAAAGGGAGAACTTTCTTCAGCAGCTGTATAAGTTCATGGAGGATAGAGGTACACCTAT
  TAACAAACGGCCTGTGCTTGGGTATCGAAATTTGAATCTTTTTAAGTTATTCAGACTTGTGCACAAACTT
  GGAGGGTTTGATAATATTGAAAGTGGAGCTGTTTGGAAGCAAGTCTATCAAGACCTTGGGATCCCTGTCT
  TAAATTCAGCTGCAGGATACAATGTTAAATGTGCTTATAAAAAGTACTTATATGGCTTTGAGGAGTACTG
  CAGGTCGGCTAATATTGACTTTCAGATGGCATTGCCAGAGAAAGTTCTTAACAAGCCATGTAAAGATTGT
  GAAAATAAAGAAGTAAAAGTTAAGGAAGAAAGTGAAACAGAGATTAAAGAAGTAAATGTGGAAGACAGTA
  AGAACGTGATGCCAAAAGAGGAGACGCCTGCTGAGGATGAAAGTGAAAGAAAAGAAAATATCAAGCCCTC
  TCTGGGAAGTAAAAAAAGCTTATTAGAATGTATACCTGCACAATCTGATGAAGAAAAAGAAGCTCACATT
  ACAAAACTCGAAGAAAATGAAAACTTGGAAGATAAAGACGGTGGCAGAGCTAGGACCGAGGAGGCCTTCA
  GCACAGAGGTAGATGGTGAAGAAGAGCAAGCACGATCTGGATATGATGAATGGATTAAAGCAGATAAAAT
  AGTAAGACCTGCTGATAAAAATGTACCAAAGATCAAACATCGGAAGAAAATAAAGAATAAACTAGACAAA
  GAAAAAGACAGAGATGAAAAATACTCTCCTAAGAACTGTAAACTTCGACGCTTGTCAAAATCACCGTTTC
  AGTCAAATCCATCTCCTGAGATGGTTTCCAAACTAGATCTTGCTGATGCCAAAAACTCTGATACAGCTCA
  TATTAAATCTATAGAAATTACTTCCATTCTTAATGGACTACAAGCTTCTGAAAGTTCGGCTGAAGACAGT
  GAGCAGGAAGATGAGAGATGTACTCAGGACGTAGATAACATTGGCAAAGACGAATCAAAGGTGGAACATT
  CAACCCACAGCAGAAATGAACTGATTTCAAAAGAGGAACAGAGTAGTCCATCTTTGCTAGAAGAAAACAA
  AGTCCATACAGATTTGGTAATAGCCAAAACAGTGTCAAAATCTCCAGAAAGATTAAGAAAAGATATGGAA
  GCAATATCGGAAGATACTGATTTTGAAGAGGAAGATGAAATCACAAAGAAGAGAAAGGATGTTAAAAAGG
  ACACAACAGACAAGGCTTTAAAGCCACAAACAAAACGTGGGAAAAGACGGTATTGCAGTGCAGATGAATG
  TTTGCAGACTGGATCCCCTGGCAAGAAGGAAGACAGAACCAAGAGTAAAGAGCCGCTCTGCACAGAAAAC
  AGTAGTAACAGCTCCTCAGATGAAGATGAAGAAGAAAAGTCCAAAGCAAAGATGACACCAACCAAGAAAT
  ATAATGGCTTAGAAGAAAAGAGAAAATCTCTGCGGACAACTAGTTTCTATTCAGGATTTTCAGAAGTAGC
  AGAAAAAAGGATAAAGCTTTTAAATAACTCTGATGAAAGACTTCAGAACAACAGGGCTAAAGATAGAAAA
  GATGTCTGGTCAAGTATTCAGGGACAGTGGCCTAAGAAAACGCTAAAGGAGCTTTTCTCTGACTCGGACA
  CTGAGGCTGCAGCTTCTCCACCCCATCCTGCCCCAGATGAGGGGGCAGTAGAGGAGTCACTGCAGACTGT
  GGCAGAAGAGGAGAGTTGTTCACCCATTATGGAGCTAGAAAAGCCACTACCTGCCAGTGTTGACAATAAG
  CCCATTGAAGAAAAACCCCTAGAAGTTAGTGATAGGAAAACAGAATTTCCGAGCAGTGGCAGTAATTCAG
  TGCTCAATACCCCACCTACCACACCAGAGTCGCCTTCTTCAGTCACTATAACAGAAGCTAGTCAGCAGCA
  GTCTTCTGTCACAGTGTCAGTGCCCCTGCCTCCAAACCAGGAAGAGGTTCGAAGCATTAAGAGTGAAACT
  GACAGCACAATTGAGGTGGACAGTGTTGTGGGGGAGCTCCAGGACCTCCAGTCGGAGGGGAATAGCTCAC
  CAGCAGGCTTTGATGCGAGCGTGAGTTCCAGCAGTAGCAATCAGCCAGAACCAGATAACCCAGAAAAAGC
  CTGTACAGGTCAGAAAAGAGTGAAAGATACTCAGGGAGTAGGAAGTTCATCAAAAAAGCAGAAGAGAAGC
  CATAAAGCCACCGTGGTGAACAACAAAAAGAAGGGAAAAGGCACAAACAGTAGTGACAGTGAAGAACTCT
  CAGCTGGTGAAAGTGTGACTAAGACTCAGACAATCAAGTCAGTCCCCACCGGAATGAAGACCCACAACAG
  CAAGTCTCCAGCAAGGGTACAGTCTCCAGGAAAAGGTGGCAGGAATGGGGATAAAGACCCTGATCTCAAG
  GAGCCTAGTAACCGGCTTCCCAAGGTTTACAAGTGGAGTTTTCAGACGTCGGACCTGGAAAATATGACAA
  GTGCTGAACGTATCTCAATTCTTCAAGAAAAATTGCAAGAAATCAGAAAGCATTATCTGTCTTTAAAATC
  TGAAGTAGCTTCCATTGATAGGAGGAGAAAACGTTTAAAGAAGAAAGAGAGAGAAAGTGCTGCTACATCT
  TCTTCCTCCTCTTCTCCCTCGTCCAGCTCCATCACAGCTGCTGTTATGTTGACACTAGCTGAACCATCAA
  TGTCCAGTGCATCACAGAATGGAATGTCAGTTGAGTGCAGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_198122
• Insert Size: 3684 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198122.2, NP_937755.1
• RefSeq Size: 5852 bp
• RefSeq ORF: 3684 bp
• Locus ID: 94246
• UniProt ID: A2CG63
• Cytogenetics: 13 5.29 cM
• Gene Summary: Acts as a transcriptional repressor. May function in the assembly and/or enzymatic activity of the Sin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes (By similarity). Plays a role in the regulation of epigenetic modifications at the PWS/AS imprinting center near the SNRPN promoter, where it might function as part of a complex with RB1 and ARID4A (PubMed:17043311). Involved in spermatogenesis, together with ARID4A, where it functions as a transcriptional coactivator for AR (androgen receptor) and enhances expression of genes required for sperm maturation. Regulates expression of the tight junction protein CLDN3 in the testis, which is important for integrity of the blood-testis barrier (PubMed:23487765). Plays a role in myeloid homeostasis where it regulates the histone methylation state of bone marrow cells and expression of various genes involved in hematopoiesis. May function as a leukemia suppressor (PubMed:18728284).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an in-frame exon in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1.