Aff1 (NM_001080798) Mouse Untagged Clone

CAT#: MC223995

Aff1 (untagged) - Mouse AF4/FMR2 family, member 1 (Aff1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Aff1
• Synonyms: 9630032B01Rik; Af; Af4; AW319193; Mllt; Mllt2h; R; Rob
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223995 representing NM_001080798
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACGAGGATTCTCCCACGAGAGAGTGGTGGAGCCTGCTTGTACAATGAAGATAGAAACCTGCTTC
  GAATCAGAGAGAAGGAAAGACGCAACCAAGAAGCTCACCAGGAGAAGGAGGCATTTCCCGAGAAGGCTCC
  CCTGTTTCCAGAGCCTTACAAGACTGCAAAAGGCGATGAGCTATCAAGTCGGATCCAGACCATGCTGGGT
  GACTATGAGGAGATGAAGGAGTTCCTGAGCAGCAAGTCCCACCCCCACCGCCTGGATGGCTCTGAAGACA
  GGCCCGGGAAGCCCAGATATCCCTTAGGTCATGACAGGGGGAACGGGGCTGCATCCAGCTCCCTCCGCAC
  ACATGTCTACCACCAGCCTATCCACACTTCTGCTCCCGGATCACGTCCTGTCGGTAACATTAGCCACAGT
  CCAAAGATGGCACAGCCAAGGATGGAGCCAAGTCTCCACACCAAAATCTATGATGGCCCACGTCTGACTC
  AAGACCACCTCAGTCAGGGACATTGTTCCAGAAAGTGTGACCGAAGAGCTGAAGGAGACTCTGCTCCCGA
  GAGGAAGCTCTCGCCCTTGATCTCCTCTTTGCCATCCCCGGTGCCCCCTCTATCACCTGTACATTCCAGG
  CTGCAGGGAACCAGCAAGGCTCACAGCAGTGGCGTTAGCAGTAAAAGCTGCTGTGTGGCCAAGTCTTCCA
  AGGACTTGGTGGCGAAGGCCCAAGATAAAGAGACTCCTCATGACGGTTTGGTGGCAGTTACCAGCCTTGG
  ATCAGCCCCTCCTCAGCCACCTTGCCAGACATTTCCACCCCCTCCTCTCCCCTCAAAAAGTGCTGCAATG
  CAGCAGAAGCCCACGGCATATGTCCGTCCCATGGACGGTCAGGATCAGGCTCCCAGTGAGTCCCCCGAGC
  TGAAGCTGCCACTGGAGGACTATGGTCAGCAAAGCTTTGAGAAACCAGACCTTAAAGTGCCTGCCAAAGC
  CAAGCTCACCAGACTAAGGATGCCCTCTCAGTCGGTGGAGCAGCCGTACTCCAATGAAGTCCATTGCGTT
  GAAGAGATTCTGAAGGAAATGACCCACTCGTGGCCACCGCCTCTGACAGCCATACACACACCCAGTACAG
  CCGAGCCGTCCAGATTTCCTTTCCCCACAAAGGACCCTCTGCACGTCAGTCCTGCAACCCAGAGCCAAAA
  ACAATATGACACGCCTTCAAAAACCCACCCCAATCCCCAGCAAGGAACCTCCATGCTGGAGGACGACCTG
  CAGCTCAGCGACAGTGAAGACAGCGACACGGAGCAAGCCACAGAGAAGCCTCCATCCCCACCTGCACCTC
  CCAGCGCTCCGCAGACACTTCCCGAGCCCGTGGCCTCAGCACATTCCAGTAGTGGGGAGTCAGAGAGCAG
  CGAGTCAGACAGTTCCTCAGACTCTGAGAGTGAGAGCAGCTCCAGCGACAGCGAGGAGGAGGAAGAGAAC
  GAGCCCCTGGAGACCCGGGCTCCCGAGCCCGAGCCTCCGACGACAAACAAATGGCAGCTGGACAACTGGT
  TGACCAAAGTCAACCAACCCTCAGTGCCCCTGGATGGCCGGGGGAGCACAGAGTCTCCACAATGGCGCCA
  GGAAAGTAAGGGTGTCGCTGAGGGCAGCAGTGACCAGCAGCATCCTGATTCCAAAGACCCTCTCCCAAAG
  AGCTCCAGCAAGACTCTCCGGGGTCCCTCTGAGGGGCCCCAGCCTGGGAAGAGGGGCTGTCCGAAATCCC
  CCGCTCAACAGGAACCCCCACCTCGGCAAACCGTGGGCAGCAAACAACCCAGGAAACCCGCCAAGGGCTC
  CGGCCAGGCAGAGCCCCAGGCCAGCTCGCAGGCAGAGAGTGAAGTAGGACCCCTTCCCTACGGCTCAAAG
  GAGCAGACTTCCAAAGACAGACCCAAGGTGAAGACGAAAGGCAGGCCGCGTGCCGTGGGCAGTCGGGAGC
  CTAAGCCAGAGGTCCCTGCGCCCACGCCTCAGGCAGCCGTGCCCAGACCGAAGCCGCCTGTGCCCACCCC
  CAGTGAGAAGAGGAAGCACAAGAGCTCCACGGCCCCCTCCAAGGCGCCCTCAGCCCCCCAGCCTCCGAAG
  GACAGTGCTGGGGACAGGAACCCAGAACACTCTGCTCTTGTCTCCTTGACTCAGAGCCAGGGTCCATCCC
  ACAGCAGCAGGGGCAGCAGTGGCAGCGTCAGGACTAGTGGCTGCCGACAGGCTGTGATTGCCCAAGGGGA
  CGGCTGTAAAGACAAGCTCCTGTTGCCTTTGAGAGACACCAAATTGCTCTCGCCGCTCAGGGACTCCCCA
  CCACCGACAAGCTTGGTGGTGAAGATAACCCTAGACCTCCTCACGAGGATTCCCCAGCCTCTGGGGAAGG
  GGAGCCGCCCCAGGAAAGCAGAAGACAAGCAGCTGTCAGCAGGGAAGAAGCAGGACTCGGAGACCAAGAG
  CTGTGACAGCTCCAGCAGAGTGACCAAGAAGAGAAAGGGTGACCCAGAAAAAGAGCACAGTAACAAGAGA
  CACAAACTGGATAAGTCGCAGACTGCGTCATCTTCATCTTCCCATACAGAATCTTCCAGAACAAAAGCGC
  CCAGGTCCTCCTCAGAGAACTCAAGAAAGGAAATGCTGCCTCCAGCATCGGCGTCCTCAGTCTCCTCTTC
  CTCATCCTCCCAGAAGCCAAGCAGACCTGCACAGAAGAGGCCAAGGCCAGACGAAGACACGTGTAGCCAG
  GAACCCCCCAGAAGTGCCAGCAGCACAAAGAGCAGCTCTACAGACCCTCCCGCTCCCAAGCACAGAAAAG
  TACAGGCCAGGGGCTCTGAACACAAGGGATCTTCTGGGGATGCTGCGAATGCTGCAAATCCTTTTCCAGT
  GCCTTCTTTGCCGAATGGTAACGCTAAACCAGGGAAGCCACAGGTGAAGTCTGACAGACAACAAGCTGAC
  TTTCACATGAAGGAGGCTAAAAAGCTGAAGTGCAAAGCAGAGACAATGGTAGACAAGGCTGGAAAAGCCT
  TCAAGTATTTGGAAGCCGTCCTCTCTTTTATCGAGTGTGGAATGGCCTCAGAGTCAGAAAGCTCAGCCAA
  GTCGGCGTACGCTGTGTACTCAGAAACCATAGACCTCATTAGGTATGTGATGTCACTAAAATGCTTCTCA
  GATAACACGATGCCAGCACAAGAGAAGATATTTGCTGTTTTATGCTTGCGTTGCCAGTCCCTTTTGAACA
  TGGCGATGTTTCGATGTAAGAAAGACACAGTGATGAAGTATTCTCGTACTCTCAGTGAACACTTCAAGAG
  TACTTCCAAAGTGGCCCAGGCACCTTCTCCATGCACGGCAAGAAGCACAGGCGTTCCGTCCCCCCTCTCC
  CCAATGCCTTCTCCTGCCAGCTCCGTGGGGTCCCAATCAAGTGCTGGCAGCAGTATGGGGAGCGTCGGGG
  TGACGGCCACCGTCAGTACTCCAGTCTCCATCCAGAACATGACCTCCTCCTATGTCACCATCACATCCCA
  TGTCCTTACGGCCTTTAGCCTTTGGGAACAGGCCGAGGCCCTCACAAGGAAGAACAAAGAATTCTTCGCT
  CAGCTCAGCACAAAAGTGCGTGTGTTGGCCCTCAACAGCAGCCTGGTGGACCTGGTGCACTACACAAGAC
  AGGGCCTGCAGCGGCTAAAACAGTCACCCAAAGGCCCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001080798
• Insert Size: 3681 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080798.2, NP_001074267.1
• RefSeq Size: 8328 bp
• RefSeq ORF: 3681 bp
• Locus ID: 17355
• Cytogenetics: 5 50.45 cM
• Gene Summary: This gene encodes a member of the AF4/ lymphoid nuclear protein related to the Fragile X E syndrome (FRAXE) family of proteins, which have been implicated in human childhood lymphoblastic leukemia, fragile chromosome X intellectual disability, and ataxia. It is the prevalent mixed-lineage leukemia fusion gene associated with spontaneous acute lymphoblastic leukemia. Members of this family have three conserved domains: an N-terminal homology domain, an AF4/ lymphoid nuclear protein domain, and a C-terminal homology domain. Knockout of the mouse gene by homologous recombination severely affects early events in lymphopoiesis, including precursor proliferation or recruitment, but is dispensable for terminal differentiation. In addition, an autosomal dominant missense mutation results in several phenotypes including ataxia and adult-onset Purkinje cell loss in the cerebellum, indicating a role in Purkinje cell maintenance and function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2017]
  Transcript Variant: This variant (1) encodes the longer isoform (1).