Sfi1 (NM_030207) Mouse Untagged Clone

CAT#: MC223978

Sfi1 (untagged) - Mouse Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sfi1
• Synonyms: 2310047I15Rik; C330046E03; mKIAA0542
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223978 representing NM_030207
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAGAAGATTGGTTCCAGGTCTTTTAGGGATGGTGTGGTTAAGAAACCTTGTTCTCCAAAGACCC
  TGCCCCTCAAAAAGTCTTCTGCCTTCTCTGGGATCCAGAGGGAGCCATCTAGAAGCTGCCACTCTATATA
  CTATCATGCCTCACAAAACTGGACTCGGTACCGATTACAAGAACTTCGAATCAGGTGTGTGGCCAGAAAG
  TTCCTGTATTTGTGGATTCGAGTGACTTTTGGAAGAGTGACTCCCTCTAGAGCCAGGATTTTCCATGAGC
  AGAAAATATTACAGAAGGTCTTCGGAGAATGGAGGGAAGAATGGTGGGTCTCCCAGAGGGAGTGGAAGCT
  CTGTGTCCGAGCTGACTGTCACTACAGATACTACTTGTACAACCTGATTTTCCAGAACTGGAAGACCTTT
  GTGCACCAGCAACGGGAGATGAGGAAGAGGTTCCGAATAGCTGAGCATCATGATACGAAGCAGAAGATGT
  GCCAAGCCTGGAAGTCCTGGTTGATATACATGGTTTCTCGTAGGACCAAACTTCACATGAAGACTACTGC
  TCTAGAGTTTAGGAGGCAGAGTGTCTTATGTTTCTGGTGGAGCAAGTGGAGGTGGCGACTAGGACAAGCC
  CATGCAGAGCACGCTCTCCATGCAGTGGCTGTGAAGCACAGGGCCCTGAGCCTCCAGCTGCAGGGTTGGC
  TGCGATGGCAAGAACAGCTTCTAATTAGCCAGCGAGATAGGAGGAAGGAGGCCACTGCTGTGCAACACTA
  TCAGCACTGGCAGAAGCAGAGATCTCTGAAGGCTTGGCTTAAATACCTCCAAATCTGCAGGGTGAAGAGA
  TGGCAGAATGAGATGGCTGTACAATTCCACCGTGCTACTGTGCTCCAGATACATTTCTGTGACTGGCAGT
  GGGCCTGGGAGTGGAGGCAGAGCTTGTCTGCCCACCAGGCCCTGGTGGTGAAGCTGGCCGGGAGGATGGT
  CCTGCGGAGGGCCTTTACACACTGGAAGCACTACATGTTGCTACAGGCAGAAGAGGCTGCCCAGCGCGAG
  GCAGCAGCTGAACACCGCCAGCACTACCTGCTGTATTCTTGCTTTAGAGCCTTCAAAGACAACGTGACCC
  AGGCCCGACTACAGCAAACAAGAAAGAAGCTTGCTCAGCAGCTGCGTGACACTACATTGCTTCACAGGTT
  CTGGAACCTCTGGCAGTCTCGAATTGAGCAGAGGGAGGAGAGGGTACAGACCCCCTCATTGCATGCAGCC
  CTGAGCCACTACAGGGTGACAGTCCTACACAAGTGTGTCAGGGTGTGGCTACGGTATGTGCACAAGAGAC
  AATGGCAGCAGTTATTGCGGGCCAGAGCAGATGGTCATTTCCAGCAGCGAGCACTGCCTGCTGCCTTCTA
  CACGTGGTACAGAGGCTGGCTATGGCACCAGCAGAGACGGATCCTGCACACAAAAGCAGTGCGTTTCCAC
  AGAGGGACATTAGAGAAGCAAGTATTTGCTCTCTGGAGGCAGAAGATGTCTCAGCATCGAGAAAACTGCT
  TGGCAGAGAGAATGGCTATACTGCAGGCAGAGCAGCAGCTTTTGCGTAGGTTCTGGTTCGTGTGGCACCA
  GCAGGCAGCAGTGTGTCAGCTGGAGCGACAGCAGCAAGCCATGGCCATTGCCCACCACCACAGTGGACTG
  CTCAGGAGGGCTTTCTGCATCTGGAAGGAAAGCACCCAAGGATTTAGGATAGAGAGGATGGGCAGGGCAC
  AGGCTGCGCACTTCCACTCTGCACAGCTCTTGAGTCGGGCCTGGAGCATGTGGAGGGAGTGCCTGGCCCT
  GAGGCTGGAAGAGCAGCAGAAACTGAAGTGTGCGGCCCTGCACAGCCAGTGCATCCTGCTTCGCAGGGCT
  CTGCAGAAGTGGCTGGTTTACCAGAATAGGGTGAGGAGTGTTCTGCGGGAGGTGGCGGCCAGGGAGAGAC
  AGCACAACAGGCAGCTGCTGTGGTGGGCGTTACATCTCTGGAGAGAAAACACCATGGCCCGTTTGGATGG
  AGCCAAAAAAACCTCTCAAGCAAGAGTTCACTACAGCAGGACTCTGTGCTCCAAGGTCCTCGTCCAGTGG
  CGAGAGGTTACTTCAGTGCAGATATATTACCGACAAAAGGAGGCAGCTGCCCTCAGGGAGGCCCGGAAAG
  CACTGGACAGGGGTCGTCTCCAGAACTGGTTTCAGCACTGGCGATTCTGCAGCCAGAGGGCAGCCCAGCA
  GAGATTCCAGCTGGGACAGGCGGCTCAGCATCACCACTGGCAGCTGCTGATGGAAGCAATGGCCAGGTGG
  AAGGCACACCATCTGGGGTGTATCAGAAAGAAGTTTCTGCAGAGGCAAGCTGCCCAACTCCTGGCACAGA
  GACTCAGTCGGGCCTGCTTCTGCCAGTGGAGGAAGCAGCTGGCAGTCCGGAAGCAAGAACAGTGGGGCAC
  AGCCCGGGCCCTGTGGCTCTGGGCCTTCTCACTACAAGCAAAGGTGTGGACTGCATGGCTGGGCTTCGTA
  CTTGAGAGGAGGAGGAAGAAGGCACGGCTGGAACGGGCTATGCAAGCCTACCAGCAGCAGCTCCTACAGG
  AGGGTGCCACACGGCTTCTGCGCTTCACAGCTGGTACGAAGGCCTTCCGGCAGCAGCTCCAGGCCCAGCA
  GCAGGTCCAGGCAGCCCACAGCCTCCACTGTGCAGTCCGCCACTGTGCTGAGCTCTGGAAAAAGAAGGTG
  CTTGGTCCAGGCAAGACTTCTCAACCACCAGCACCCACCACATTCAGCAAGAGAGTGACATTTAAGGATT
  CTTTCCTCTCTGGACATGCTGCGGAGGCTGGAGATGCTACCCAGGAGACAAAGAAGCTTCGAGCTCCTCC
  ATCTCAGGGAGTCCTGGGTAGTTTGGCAGGAGCTGCTGGGGAGCCCTGCCACCTAGACCTCAATGCAGCC
  CGCTCATCACGGAAACAGCCCCGACGTCCATCCTTCCTGCTTGAGCGTTTGGGGAGTCAGAGGTCCCCAG
  AGTGGTACAGCCTTGGGGAGCAGCAGTTAGAGAAGCCCCCAGAGGAGGAAAGCACGGCTCTGCTGGGAGG
  CTCTTCCCTGACAAGACCCTTCCTACCTGGGGTCCTGCCGAATGTTCCTGGTCCGAAGCTGCCTCCTACG
  GCCAGTCCAGGCCTGGAGCTGCTGCCTCCCTCCTCCATCATGCCCCATGCGGCAGGAGGCACTGCTAGGG
  TGTCAGCGAAGCCCAGCATCCCTGGGCCTCAGCCTTGGGGGTGCCCGTCCTTACCCAGGGACCTTGATCC
  CCAACTCCTCCCTGGAGATTCCATAAGCACCCGGACTGAGCCTGTTTATGGCTCAGAGGCTACAGGCCAC
  ACAGAGCTTGAGGCTGAGTTGGAGGGGATCCAGCAGCAGTTACAACACTACCAAACCACCAAGCAGAACC
  TTTGGTCATGCCAGCGACAAGCCAACAGCCTGCGTAGGTGGCTGGAGCTAAGCCAGGAGGAGCCGAAATC
  CGAGGACCTTCACCTAGAAGAGCAGGTGAAAACAGAACTGGAGGAGGTGGAACTACAGGTCCAGCAGCTG
  GCTAAGGAGCTTGAGGCCCAGCGTCAGCCTGTTGGTACATGCATTGCTCGTGTTCGGGCCCTGAGACGGG
  CACTGTGCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_030207
• Insert Size: 3651 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030207.2, NP_084483.2
• RefSeq Size: 4025 bp
• RefSeq ORF: 3651 bp
• Locus ID: 78887
• UniProt ID: Q3UZY0
• Cytogenetics: 11 A1
• Gene Summary: Plays a role in the dynamic structure of centrosome-associated contractile fibers via its interaction with CETN2.[UniProtKB/Swiss-Prot Function]