Itsn1 (NM_001110275) Mouse Untagged Clone

CAT#: MC223966

Itsn1 (untagged) - Mouse intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Itsn1
• Synonyms: AA517634; AA545208; AI316805; AI839402; AI848451; Ehsh1; Ese1; Itsn; Sh3p17
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223966 representing NM_001110275
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCAGTTTCCCACACCTTTCGGTGGTAGCCTGGATGTCTGGGCCATAACTGTGGAGGAAAGGGCCA
  AGCATGACCAGCAGTTCCTTAGCCTGAAGCCGATAGCGGGATTTATTACTGGTGATCAAGCGAGGAACTT
  TTTTTTCCAATCTGGGTTACCTCAGCCTGTCTTAGCACAAATATGGGCGCTAGCGGACATGAATAACGAT
  GGAAGGATGGATCAAGTGGAATTTTCCATAGCCATGAAGCTTATCAAACTGAAGCTACAAGGATATCAGC
  TCCCCTCCACACTTCCCCCTGTCATGAAACAGCAACCAGTGGCTATTTCCAGTGCACCAGCATTTGGTAT
  AGGAGGGATTGCTAGCATGCCACCACTCACAGCTGTTGCTCCTGTGCCAATGGGCTCCATTCCAGTTGTT
  GGAATGTCTCCACCCTTAGTATCTTCTGTCCCTCCAGCAGCAGTGCCTCCCCTGGCTAACGGGGCTCCTC
  CCGTCATACAGCCTCTGCCTGCGTTTGCGCATCCTGCAGCCACATTGCCAAAGAGTTCTTCCTTCAGCAG
  ATCTGGTCCAGGGTCACAATTAAACACTAAGTTACAGAAGGCACAATCATTCGATGTCGCCAGCGCCCCT
  CCAGCAGCAGAATGGGCTGTGCCTCAGTCATCAAGGCTGAAATACAGGCAGTTATTCAACAGCCACGACA
  AAACTATGAGTGGACACTTAACAGGTCCCCAGGCAAGAACTATTCTCATGCAATCAAGTTTACCCCAGGC
  TCAGCTGGCTTCAATATGGAATCTTTCTGACATTGATCAAGATGGAAAACTCACTGCAGAAGAATTTATC
  CTAGCTATGCACCTAATTGATGTTGCCATGTCTGGTCAGCCACTGCCGCCCGTCCTGCCTCCAGAATACA
  TCCCTCCTTCCTTCAGAAGAGTTCGCTCCGGCAGTGGGATGTCCGTCATAAGCTCTTCTTCTGTGGATCA
  GAGGCTGCCTGAGGAGCCGTCGTCAGAGGATGAGCAGCAGCCAGAGAAGAAACTGCCTGTGACATTTGAA
  GATAAGAAGCGGGAGAACTTCGAGCGAGGCAGTGTGGAGCTGGAGAAGCGCCGCCAAGCGCTCTTGGAGC
  AGCAGCGCAAAGAGCAGGAGCGGTTGGCTCAGCTGGAGCGCGCCGAGCAGGAGAGGAAAGAGCGGGAGCG
  CCAGGAGCAGGAGCGCAAGCGGCAGCTGGAGCTGGAGAAGCAGCTGGAGAAGCAGCGGGAGCTGGAGCGG
  CAGCGAGAGGAGGAGAGGAGGAAGGAGATCGAGAGGCGCGAGGCCGCAAAACGGGAACTGGAAAGGCAGC
  GACAACTTGAATGGGAACGGAACCGGAGACAGGAACTCCTGAATCAGAGGAACAAGGAGCAGGAGGGCAC
  CGTGGTCCTGAAGGCAAGGAGGAAGACTCTGGAGTTTGAGTTAGAAGCTCTGAATGACAAAAAGCATCAG
  CTAGAAGGAAAACTTCAGGATATCAGGTGTCGACTGGCAACCCAGAGGCAAGAAATTGAGAGCACGAACA
  AGTCTAGAGAGCTAAGAATTGCTGAAATCACCCACTTACAGCAGCAGTTGCAGGAATCTCAGCAAATGCT
  TGGAAGACTTATTCCAGAGAAACAGATACTCAGTGACCAGTTAAAACAAGTCCAGCAGAACAGTTTGCAT
  AGAGACTCGCTTCTTACCCTCAAAAGAGCCTTGGAAGCAAAGGAGCTGGCCCGGCAGCAGCTCCGGGAGC
  AGCTGGACGAGGTGGAGAGAGAGACCAGGTCAAAGCTGCAGGAGATTGATGTTTTCAACAACCAGCTGAA
  GGAACTGAGAGAGATACATAGCAAACAGCAACTCCAGAAGCAGAGGTCCCTGGAGGCAGCGCGACTGAAG
  CAGAAAGAGCAGGAGAGGAAGAGCCTGGAGTTAGAGAAGCAAAAGGAAGACGCTCAGAGACGAGTTCAGG
  AAAGGGACAAGCAATGGCTGGAGCATGTGCAGCAGGAGGAGCAGCCACGCCCCCGGAAACCCCACGAGGA
  GGACAGACTGAAGAGGGAAGACAGTGTCAGGAAGAAGGAGGCGGAAGAGAGAGCCAAGCCGGAAATGCAA
  GACAAGCAGAGTCGGCTTTTCCATCCGCATCAGGAGCCAGCTAAGCTGGCCACCCAGGCACCCTGGTCTA
  CCACAGAGAAAGGCCCGCTTACCATTTCTGCACAGGAGAGTGTAAAAGTGGTATATTACCGAGCGCTGTA
  CCCCTTTGAATCCAGAAGTCACGATGAGATCACCATCCAGCCAGGAGATATAGTCATGGTGGATGAAAGC
  CAGACTGGAGAGCCAGGATGGCTTGGAGGAGAGCTGAAAGGGAAGACGGGATGGTTCCCTGCAAACTATG
  CAGAAAAGATTCCAGAAAATGAGGTTCCCACTCCAGCCAAACCAGTGACCGATCTGACATCTGCCCCTGC
  CCCCAAACTGGCTCTGCGTGAGACCCCTGCTCCTTTGCCAGTGACCTCTTCTGAGCCCTCCACAACCCCC
  AACAACTGGGCAGACTTCAGTTCCACGTGGCCCAGCAGCTCAAACGAGAAGCCAGAAACGGACAACTGGG
  ATACATGGGCGGCTCAGCCTTCTCTGACCGTACCTAGTGCTGGCCAGTTACGGCAGAGATCAGCCTTTAC
  CCCAGCCACAGCCACTGGCTCCTCCCCATCTCCCGTCCTGGGCCAGGGTGAAAAGGTGGAAGGGCTACAA
  GCGCAAGCCCTGTATCCCTGGAGAGCCAAAAAAGACAACCACTTAAATTTTAACAAAAGTGACGTCATCA
  CCGTTCTGGAACAGCAAGACATGTGGTGGTTTGGAGAAGTTCAAGGTCAGAAGGGTTGGTTCCCCAAGTC
  TTACGTGAAACTCATTTCAGGGCCCGTAAGGAAATCCACAAGCATCGATACTGGCCCTACTGAAAGTCCT
  GCTAGTCTAAAGAGAGTGGCTTCCCCGGCCGCCAAGCCAGCCATTCCCGGAGAAGAGTTTATTGCCATGT
  ACACATACGAGAGTTCTGAGCAAGGAGATTTAACCTTTCAGCAAGGGGATGTGATTGTGGTTACCAAGAA
  AGATGGTGACTGGTGGACGGGAACGGTGGGCGACAAGTCCGGAGTCTTCCCTTCTAACTATGTGAGGCTT
  AAAGATTCAGAGGGCTCTGGAACTGCTGGGAAAACAGGGAGTTTAGGAAAAAAACCTGAAATTGCCCAGG
  TTATTGCTTCCTACGCTGCTACTGGTCCCGAACAACTCACCCTGGCTCCTGGGCAGCTGATTCTGATCCG
  GAAAAAGAACCCAGGTGGATGGTGGGAAGGAGAACTGCAAGCTCGAGGGAAAAAGCGCCAGATAGGGTGG
  TTTCCAGCAAATTATGTCAAACTTCTAAGCCCCGGAACAAGCAAAATCACCCCAACTGAGCTACCCAAGA
  CCGCAGTGCAGCCAGCAGTGTGCCAGGTGATCGGGATGTACGATTACACCGCCCAGAACGATGACGAACT
  AGCCTTCAGCAAAGGCCAGATCATCAACGTCCTCAACAAGGAGGACCCGGACTGGTGGAAAGGAGAAGTC
  AGTGGGCAAGTTGGGCTCTTCCCATCCAATTATGTAAAGCTGACCACAGACATGGACCCCAGCCAGCAAT
  GA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001110275
• Insert Size: 3642 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001110275.1, NP_001103745.1
• RefSeq Size: 5386 bp
• RefSeq ORF: 3642 bp
• Locus ID: 16443
• UniProt ID: Q9Z0R4
• Cytogenetics: 16 C4
• Gene Summary: Adapter protein that provides a link between the endocytic membrane traffic and the actin assembly machinery (PubMed:10064583). Acts as guanine nucleotide exchange factor (GEF) for CDC42, and thereby stimulates actin nucleation mediated by WASL and the ARP2/3 complex (By similarity). Plays a role in the assembly and maturation of clathrin-coated vesicles (By similarity). Recruits FCHSD2 to clathrin-coated pits (By similarity). Involved in endocytosis of activated EGFR, and probably also other growth factor receptors (PubMed:16914641). Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR may involve association with DAB2 (By similarity). Promotes ubiquitination and subsequent degradation of EGFR, and thereby contributes to the down-regulation of EGFR-dependent signaling pathways (PubMed:16914641). In chromaffin cells, required for normal exocytosis of catecholamines (PubMed:18676989). Required for rapid replenishment of release-ready synaptic vesicles at presynaptic active zones (PubMed:23633571). Inhibits ARHGAP31 activity toward RAC1 (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) has multiple differences in the presence and absence of exons at its 3' end, compared to variant 1. These differences produce a unique 3' UTR and result in a protein (isoform 2) that is shorter at the C-terminus, compared to isoform 1.