Trpm4 (NM_175130) Mouse Untagged Clone

CAT#: MC223963

Trpm4 (untagged) - Mouse transient receptor potential cation channel, subfamily M, member 4 (Trpm4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trpm4
• Synonyms: 1110030C19Rik; AW047689; LTrpC-4; LTRPC4; TRPM4B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223963 representing NM_175130
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGGGCCGGAGAAGGAGCAGAGCTGGATCCCTAAGATCTTCAGGAAGAAGGTGTGCACGACGTTCA
  TAGTGGACCTCTCCGATGATGCGGGAGGGACCTTGTGCCAGTGTGGGCAACCTCGGGATGCCCACCCTTC
  TGTGGCTGTGGAGGATGCCTTTGGGGCAGCCGTAGTTACCGAGTGGAACAGTGATGAGCACACCACGGAG
  AAGCCCACAGATGCCTATGGGGACCTGGACTTCACGTACTCTGGCCGAAAGCACAGCAACTTTCTCCGGC
  TGTCTGACCGCACGGATCCGGCCACTGTGTACAGTCTCGTTACCCGCTCTTGGGGCTTTCGTGCCCCAAA
  CTTGGTGGTGTCGGTGCTGGGGGGGTCGGGGGGCCCCGTGCTCCAGACCTGGCTGCAGGACCTTCTGCGT
  CGCGGGCTGGTGAGAGCCGCCCAGAGCACAGGGGCCTGGATCGTCACTGGGGGACTGCACACAGGCATTG
  GCCGGCACGTGGGTGTGGCTGTAAGGGACCACCAGACAGCCAGCACTGGGAGCAGCAAGGTGGTGGCCAT
  GGGTGTGGCCCCCTGGGGAGTGGTCCGGAACAGAGACATGCTGATCAACCCCAAGGGCTCATTCCCCGCA
  AGGTACCGGTGGCGTGGTGACCCTGAGGATGGTGTTGAGTTCCCCCTGGACTATAACTATTCTGCTTTCT
  TCTTGGTGGATGATGGCACCTATGGCCGCCTGGGTGGTGAGAACCGCTTCCGCCTTCGGTTTGAGTCCTA
  TGTGGCTCAGCAGAAGACCGGAGTGGGAGGGACTGGAATTGACATCCCAGTGCTCCTTCTTCTCATCGAT
  GGAGATGAGAAGATGTTAAAGCGGATAGAGGATGCCACCCAAGCTCAGCTCCCCTGCCTCTTGGTGGCGG
  GCTCTGGGGGTGCTGCAGACTGTCTGGTGGAGACCCTGGAAGATACTCTGGCCCCAGGGAGTGGGGGACT
  TAGGCGAGGCGAAGCCCGGGATCGGATTAGGCGATACTTCCCTAAAGGAGACCCTGAGGTTCTTCAGGCC
  CAGGTAGAGAGGATCATGACCCGAAAGGAGTTGCTGACGGTCTATTCATCAGAAGACGGCTCCGAGGAGT
  TTGAGACTATCGTTTTGAGGGCTCTTGTGAAAGCCTGTGGGAGCTCTGAGGCCTCAGCCTACCTGGATGA
  GCTGCGTTTGGCTGTGGCTTGGAACCGCGTGGACATCGCCCAAAGTGAACTTTTCCGTGGGGACATCCAA
  TGGCGGTCCTTCCACCTGGAGGCATCTCTCATGGATGCTCTGCTGAATGACCGGCCTGAATTTGTGCGCC
  TGCTCATCTCCCATGGCCTTAGCCTGGGACACTTCCTGACCCCCGTGCGTCTGGCCCAGCTGTACAGCGC
  GGTGTCCCCTAACTCACTGATCCGAAATCTTCTGGACCAGGCGTCCCATGCTAGTAGCAGCAAATCCCCT
  CCTGTAAATGGAACTGTAGAGCTCCGGCCTCCCAATGTGGGGCAAGTGCTGAGGACTCTGTTGGGAGAAA
  CGTGTGCACCACGGTACCCTGCCAGGAACACCCGAGACTCCTATCTGGGCCAGGACCACAGAGAGAATGA
  CTCTCTGCTTATGGACTGGGCCAACAAGCAACCATCAACGGATGCAAGCTTTGAACAAGCCCCCTGGAGT
  GACCTGCTTATTTGGGCTCTGTTGCTGAACCGGGCCCAGATGGCTATCTATTTCTGGGAGAAGGGCTCCA
  ACTCAGTGGCCTCTGCTCTTGGGGCTTGTCTTTTACTCCGAGTGATGGCTCGCCTAGAGTCGGAGGCTGA
  GGAGGCCGCGCGGCGGAAGGATCTTGCTGCCACGTTTGAAAGCATGAGCGTGGACCTCTTTGGAGAGTGT
  TACCACAACAGTGAAGAGCGAGCAGCCCGCCTTCTTCTGCGCCGCTGTCCCCTCTGGGGAGAGGCCACCT
  GCCTCCAGCTTGCCATGCAGGCTGATGCCCGAGCGTTCTTTGCCCAGGATGGAGTCCAGTCTCTTCTGAC
  ACAGAAGTGGTGGGGGGAGATGGACAGCACAACTCCCATCTGGGCCCTGCTTCTCGCCTTCTTCTGCCCG
  CCTCTCATCTACACCAACCTTATCGTCTTCAGGAAGTCAGAGGAGGAGCCCACACAGAAGGATCTTGACT
  TTGATATGGACAGCAGCATCAATGGAGCAGGTCCTCCTGGGACTGTAGAACCCTCTGCAAAGGTGGCCCT
  TGAGAGGCGGCAGCGGCGACGGCCAGGACGTGCCCTCTGCTGTGGCAAGTTCTCCAAGCGCTGGTCGGAC
  TTCTGGGGTGCCCCAGTGACTGCCTTCCTGGGTAATGTGGTCAGTTACCTGCTGTTCCTGCTGCTGTTTG
  CCCACGTGTTGCTGGTGGATTTCCAGCCCACAAAGCCAAGTGTCTCCGAGCTGCTGCTGTACTTCTGGGC
  CTTCACGTTGCTGTGCGAGGAGCTGCGCCAGGGCCTGGGTGGTGGCTGGGGTAGCCTGGCCAGTGGGGGA
  CGTGGTCCGGACAGAGCTCCCCTGCGCCATCGGCTGCACCTCTACCTCTCAGATACCTGGAACCAATGTG
  ACCTGCTGGCACTCACCTGCTTCCTGCTGGGCGTAGGCTGCCGGCTGACCCCTGGGCTCTTTGACTTGGG
  ACGCACCGTCCTCTGCCTTGACTTCATGATCTTCACACTGCGCCTGCTGCACATCTTCACGGTGAACAAG
  CAGCTGGGGCCCAAGATTGTCATAGTGAGCAAAATGATGAAGGATGTATTCTTCTTCCTCTTCTTCCTCT
  GCGTGTGGCTTGTAGCTTATGGGGTGGCCACAGAGGGGATCCTGAGGCCCCAGGACCGCAGTCTACCGAG
  TATCCTGCGCAGGGTCTTCTACCGACCGTATCTGCAGATCTTCGGACAAATTCCCCAGGAGGAAATGGAC
  GTGGCCCTCATGATTCCAGGTAACTGCTCAATGGAGCGGGGCTCCTGGGCTCATCCGGAGGGGCCCGTCG
  CAGGCTCCTGTGTGTCCCAGTATGCCAACTGGCTGGTGGTGTTGCTCCTTATCGTCTTCTTGCTGGTGGC
  CAATATCCTGTTGCTCAACCTGCTCATCGCCATGTTCAGCTACACCTTCAGCAAAGTGCACGGCAACAGC
  GACCTCTACTGGAAGGCACAGCGCTACAGCCTCATCCGGGAATTCCATTCGAGGCCTGCCCTGGCCCCAC
  CCCTCATCATCATCTCTCATGTGCGCCTCCTCATCAAGTGGCTGCGCAGGTGTCGCAGGTGTCGCAGGGC
  TAACTTGCCCGCCTCCCCAGTCTTCGAGCACTTCCGTGTCTGTCTCTCTAAGGAAGCAGAGCGGAAGCTG
  CTGACTTGGGAGTCTGTGCACAAAGAGAACTTCCTGTTGGCACAAGCTCGGGACAAGCGAGACAGTGACT
  CGGAGCGTTTGAAACGCACGTCTCAGAAGGTGGACACTGCACTGAAGCAGCTGGGACAGATCAGAGAGTA
  CGATCGGCGTCTGAGGGGGCTGGAGAGAGAGGTCCAACACTGTTCTCGAGTCCTGACTTGGATGGCTGAG
  GCCCTTAGCCACTCTGCCTTGCTGCCTCCAGGGGCCCCACCCCCTCCGAGCCCCACTGGTTCCAAAGACT
  GA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175130
• Insert Size: 3642 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175130.4, NP_780339.2
• RefSeq Size: 4245 bp
• RefSeq ORF: 3642 bp
• Locus ID: 68667
• UniProt ID: Q7TN37
• Cytogenetics: 7 B3
• Gene Summary: Calcium-activated non selective (CAN) cation channel that mediates membrane depolarization. While it is activated by increase in intracellular Ca(2+), it is impermeable to it (PubMed:17188667, PubMed:29211714). Mediates transport of monovalent cations (Na(+) > K(+) > Cs(+) > Li(+)), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca(2+) oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca(2+) overload. Affects T-helper 1 (Th1) and T-helper 2 (Th2) cell motility and cytokine production through differential regulation of calcium signaling and NFATC1 localization. Enhances cell proliferation through up-regulation of the beta-catenin signaling pathway (By similarity). Essential for the migration but not the maturation of dendritic cells (PubMed:18758465).[UniProtKB/Swiss-Prot Function]