Smc3 (NM_007790) Mouse Untagged Clone

CAT#: MC223961

Smc3 (untagged) - Mouse structural maintenace of chromosomes 3 (Smc3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Smc3
• Synonyms: Bamacan; Cspg6; HCAP; Mmip1; SMC-3; SmcD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223961 representing NM_007790
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACATCAAGCAGGTGATCATCCAGGGCTTCCGAAGTTACCGAGACCAAACAATTGTAGATCCCTTCA
  GCTCCAAACATAATGTCATCGTGGGCAGAAATGGCTCTGGAAAAAGCAACTTCTTTTATGCAATCCAGTT
  TGTTCTCAGTGATGAATTCAGCCATCTTCGTCCAGAGCAGCGATTGGCTTTGTTGCATGAGGGTACAGGT
  CCTCGTGTTATTTCTGCTTTTGTGGAAATCATTTTTGACAATTCGGACAACCGGTTACCAATTGATAAAG
  AGGAAGTTTCACTTCGAAGAGTTATTGGTGCCAAAAAGGATCAGTATTTCCTAGATAAGAAGATGGTCAC
  GAAAAATGATGTGATGAATCTCCTTGAAAGTGCTGGGTTTTCCAGAAGTAATCCTTATTACATTGTTAAA
  CAAGGAAAGATCAACCAAATGGCAACGGCACCAGATTCTCAGAGATTAAAGCTGTTGAGAGAAGTAGCTG
  GTACTAGAGTGTATGATGAACGGAAAGAAGAAAGCATCTCCCTGATGAAAGAAACAGAGGGCAAACGGGA
  AAAGATCAATGAGTTGTTGAAGTACATTGAAGAGCGCCTGCACACTCTAGAGGAGGAGAAGGAGGAGCTG
  GCCCAGTATCAGAAGTGGGATAAGATGAGGCGCGCCCTGGAGTACACCATCTACAACCAGGAGCTCAACG
  AGACGCGCGCTAAGCTCGACGAGCTTTCTGCTAAGCGAGAAACGAGTGGAGAGAAATCCAGACAACTAAG
  GGATGCCCAGCAGGATGCAAGAGACAAAATGGAGGATATTGAGCGCCAGGTTAGAGAACTGAAAACAAAA
  ATTTCAGCCATGAAAGAAGAAAAAGAACAGCTTAGTGCTGAAAGACAAGAGCAGATTAAGCAAAGGACCA
  AGTTGGAGCTTAAAGCCAAGGATTTACAAGATGAGCTGGCGGGCAACAGTGAACAGCGGAAACGTTTATT
  AAAAGAGAGGCAGAAGCTGCTTGAAAAAATAGAAGAAAAGCAGAAAGAACTGGCAGAAACAGAACCTAAA
  TTCAACAGCGTAAAAGAAAAAGAAGAGCGAGGAATTGCGAGGTTGGCTCAAGCTACACAGGAAAGAACTG
  ATCTTTATGCAAAGCAGGGTCGAGGAAGCCAGTTTACGTCAAAAGAAGAAAGGGATAAATGGATTAAAAA
  GGAACTGAAGTCTTTAGATCAGGCAATTAATGATAAGAAAAGACAGATTGCTGCTATTCACAAGGATTTG
  GAGGATACCGAGGCAAATAAAGAGAAAAATCTGGAGCAATATAATAAATTGGATCAGGATCTCAATGAAG
  TCAAAGCTCGAGTTGAAGAACTGGACAGAAAATATTATGAAGTAAAAAATAAGAAAGATGAACTACAAAG
  TGAAAGAAATTACTTGTGGAGAGAGGAGAATGCAGAACAACAAGCACTTGCTGCTAAAAGAGAAGACCTT
  GAGAAGAAGCAGCAACTTCTTAGAGCGGCAACAGGAAAGGCCATTTTAAATGGAATAGATAGCATTAACA
  AAGTGCTAGAACATTTTCGGCGAAAAGGTATAAACCAGCATGTTCAAAATGGCTACCATGGCATCGTAAT
  GAATAACTTTGAGTGTGAACCAGCTTTCTATACTTGTGTGGAAGTCACTGCTGGTAACAGGTTATTTTAT
  CACATTGTTGATTCAGATGAAGTCAGCACGAAAATTTTAATGGAGTTCAATAAAATGAATCTTCCTGGAG
  AGGTGACTTTCCTGCCTCTTAACAAGTTAGATGTGAGAGATACTGCCTATCCTGAAACTAACGATGCTAT
  TCCTATGATCAGTAAGCTGAGGTACAATCCCAGATTTGACAAAGCTTTCAAACATGTATTTGGAAAGACA
  CTCATCTGTCGGAGCATGGAAGTTTCAACTCAGCTGGCCCGGGCCTTCACTATGGACTGTATTACTTTGG
  AAGGTGATCAAGTCAGCCATCGAGGTGCTCTGACTGGAGGTTATTACGACACAAGAAAGTCTCGACTTGA
  GTTACAGAAAGACGTTAGGAAGGCAGAGGAGGAGCTGGGTGAGCTGGAGGCTAAGCTCAATGAAAACCTA
  CGCAGGAACATTGAAAGGATTAATAATGAAATTGACCAGTTGATGAACCAAATGCAGCAGATAGAGACCC
  AACAAAGAAAATTTAAAGCATCCAGAGATAGCATATTATCAGAGATGAAGATGCTAAAAGAGAAGAGACA
  GCAATCAGAAAAGACCTTCATGCCAAAGCAACGTAGCTTACAAAGCTTGGAGGCAAGTCTGCATGCTATG
  GAGTCCACCAGAGAGTCACTGAAAGCGGAGCTAGGAACGGATTTGCTTTCTCAACTCAGTCTGGAAGATC
  AGAAAAGAGTCGACGCTCTGAATGATGAAATCCGTCAGCTGCAGCAGGAAAACAGACAGCTGCTAAATGA
  AAGAATTAAACTAGAAGGCATTATTACTCGAGTAGAGACTTACCTGAATGAGAATCTGAGGAAACGCTTG
  GACCAAGTAGAACAGGAACTTAATGAACTGAGAGAGACAGAAGGTGGTACTGTTCTTACTGCCACAACAT
  CAGAACTTGAAGCTATTAATAAAAGAGTAAAAGATACTATGGCAAGATCAGAAGATTTGGACAATTCCAT
  TGACAAAACAGAAGCTGGAATTAAAGAGCTCCAGAAAAGTATGGAGCGCTGGAAAAATATGGAGAAAGAA
  CACATGGATGCCATAAATCATGATACTAAAGAGCTGGAGAAGATGACCAACCGGCAAGGCATGCTGTTGA
  AGAAGAAGGAGGAGTGTATGAAGAAGATCCGGGAGCTGGGGTCCCTCCCCCAGGAAGCGTTTGAGAAGTA
  CCAGACACTGAGCCTGAAGCAGTTGTTTCGAAAACTGGAGCAATGCAACACAGAGTTAAAGAAGTACAGC
  CACGTGAACAAAAAGGCTCTAGATCAGTTTGTGAATTTCTCTGAGCAGAAGGAAAAGCTGATAAAGCGGC
  AAGAGGAATTGGATAGAGGCTACAAATCAATCATGGAATTGATGAATGTACTTGAACTTAGAAAATATGA
  AGCTATTCAGTTAACTTTCAAACAGGTATCTAAGAACTTCAGTGAAGTTTTCCAGAAGTTGGTACCTGGT
  GGCAAAGCGACTTTGGTGATGAAGAAAGGAGATGTGGAGGGCAGCCAGTCTCAGGACGAAGGAGAAGGGA
  GTGGCGAGAGTGAGCGGGGCTCTGGGTCACAGAGCAGTGTTCCATCAGTTGACCAGTTCACAGGAGTTGG
  GATCAGGGTGTCATTTACAGGGAAGCAAGGGGAGATGAGAGAAATGCAGCAGCTCTCAGGAGGACAGAAA
  TCTCTGGTAGCCCTTGCTCTCATCTTTGCCATTCAGAAATGCGACCCTGCTCCTTTTTACCTGTTTGATG
  AGATCGACCAAGCTTTGGATGCTCAGCACAGAAAAGCCGTGTCAGATATGATTATGGAGCTAGCCGTGCA
  TGCCCAGTTTATTACCACTACTTTCAGGCCTGAACTGCTTGAGTCAGCTGACAAATTCTATGGTGTAAAG
  TTCAGAAATAAGGTTAGTCACATTGATGTGATCACAGCAGAGATGGCCAAAGATTTTGTAGAAGACGATA
  CCACGCATGGTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_007790
• Insert Size: 3654 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007790.3, NP_031816.2
• RefSeq Size: 4851 bp
• RefSeq ORF: 3654 bp
• Locus ID: 13006
• UniProt ID: Q9CW03
• Cytogenetics: 19 D2
• Gene Summary: Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.[UniProtKB/Swiss-Prot Function]