Slc12a2 (NM_009194) Mouse Untagged Clone

CAT#: MC223940

Slc12a2 (untagged) - Mouse solute carrier family 12, member 2 (Slc12a2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Slc12a2
• Synonyms: 9330166H04Rik; mBSC2; Nkcc1; sy-ns
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223940 representing NM_009194
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCGGGCCCGCGGCGCCCTCGTCTGGCGCCCCGCGGCCCGCCCGGGATGGGGACGCGCCGCTGG
  CTGCCGCCGCCGGGGTGGACCTGCCCGGCACGGCCGTGCCCTCCGGCCAAGAGGATGCTACTACGGCTGG
  GAGGCAGGCTGGCGGCGGGGTGCGCGGAGAGGGAACCCCAGCGGCGGGCGATGGGCTGGGCAGACCTCTG
  GGGCCCACCCCCAGCCAGAGCCGCTTCCAGGTGGACCCGGTTTCCGAGAACGCCGGGCGGGCCGCGGCGG
  CTGCGGCGGCGGCGGCGGCGGCTGCGGCTGCGGCCGGGGCGGCGGGCAAGGAGACCCCCGCGGCCGGGAA
  AGCCGGCGGTGAGAGCGGCGTGGCCAAGGGCAGCGAGGAAGCCAAGGGCCGCTTCCGCGTGAACTTCGTG
  GACCCGGCTGCCTCGTCGTCGGCGGACGACAGCCTGTCGGATGCGGCGGGGGTCGGTGGCGACGGGCCCA
  ACGTGAGCTTCCAGAACGGCGGGGACACGGTGCTGAGCGAGGGCAGCAGCCTGCACTCGGGGGGTGGCAG
  TGGACACCACCAGCAGTACTACTATGACACCCACACCAACACCTACTACCTGCGCACCTTCGGCCACAAC
  ACCATGGACGCGGTGCCCAGGATCGACCACTACCGGCACACGGCCGCGCAGCTGGGCGAGAAGCTGCTCC
  GGCCCAGCCTGGCAGAGCTCCACGATGAGCTGGAAAAGGAACCTTTTGAGGATGGCTTTGCGAATGGAGA
  AGAAAGTACTCCAACCAGAGATGCTGTGGTCGCATACACTGCCGAGAGTAAAGGAGTTGTAAAGTTTGGC
  TGGATCAAGGGTGTATTAGTCCGCTGCATGTTAAACATTTGGGGTGTGATGCTCTTTATTAGATTGTCAT
  GGATCGTGGGTCAAGCTGGGATAGGTCTCTCTGTCGTCGTAATAGCAATGGCCACTGTTGTGACAACTAT
  CACAGGATTGTCTACTTCAGCAATAGCTACCAATGGGTTCGTGAGAGGAGGAGGAGCGTACTATTTAATC
  TCTAGAAGTCTAGGGCCGGAATTTGGTGGTGCAATTGGCTTGATCTTTGCTTTTGCCAATGCGGTGGCCG
  TTGCTATGTATGTTGTCGGATTCGCAGAGACTGTGGTGGAGCTGCTTAAGGAACATTCCATACTTATGAT
  AGATGAAATCAATGATATCCGAATTATTGGAGCCATTACAGTCGTGATTCTTCTGGGCATCTCAGTGGCT
  GGAATGGAGTGGGAAGCAAAGGCTCAGATTGTGCTGCTGGTGATCCTTCTCCTGGCCATCGCCGACTTCG
  TCATAGGGACGTTTATCTCACTGGAGAGCAAGAAGCCCAAGGGTTTCTTTGGCTATAAATCTGAAATATT
  TAATGAGAACTTTGGGCCGGATTTTCGAGAAGAAGAGACTTTCTTCTCTGTATTTGCCATCTTTTTTCCT
  GCTGCAACTGGTATTCTAGCTGGAGCAAATATCTCAGGTGATCTTGCAGATCCTCAGTCAGCCATACCCA
  AAGGAACACTCTTAGCCATTTTGATTACTACAGTGGTTTACATAGGAATTGCAGTGTCTGTGGGTTCGTG
  TGTTGTTCGGGATGCCACTGGCAATGTTAATGATACCATTACAACAGAGCTGACAAACTGTACTTCTGCA
  GCCTGCAAACTAAACTTTGACTTCTCTTATTGTGAGAGCAATACTTGTTCTTACGGGCTAATGAACAACT
  TTCAGGTGATGAGCATGGTGTCAGGATTTGCACCCTTGATCTCTGCAGGGATCTTTTCAGCCACGCTCTC
  GTCAGCACTAGCATCTCTTGTGAGTGCTCCCAAAATATTTCAGGCTCTATGTAAGGACAATATCTACCCA
  GCTTTCCAGATGTTTGCTAAAGGTTATGGGAAAAATAATGAACCGCTTCGTGGTTACATCTTAACATTTC
  TGATTGCACTGGGATTCATCCTGATTGCTGAATTGAACGTTATTGCTCCAATTATCTCTAATTTCTTCCT
  TGCATCGTATGCCTTAATCAACTTTTCAGTATTCCATGCATCACTTGCAAAATCTCCAGGATGGCGTCCT
  GCATTCAAATACTACAACATGTGGATCTCCCTTATTGGAGCAATTCTTTGTTGCATAGTGATGTTTGTCA
  TTAACTGGTGGGCTGCGTTGCTCACATATGTGATAGTCCTTGGGCTGTACATTTATGTTACTTACAAAAA
  ACCAGATGTGAACTGGGGATCCTCCACACAAGCGCTGACTTACCTCAGTGCACTGCAGCATTCAATCCGT
  CTTTCTGGGGTGGAGGACCATGTGAAAAACTTCAGACCACAGTGCCTTGTAATGACAGGTTCTCCAAACT
  CACGGCCTGCTTTACTTCATCTTGTTCATGACTTCACGAAAAATGTTGGTTTAATGATCTGTGGCCATGT
  GCATATGGGCCCCAGAAGACAAGCTATGAAAGAGATGTCCATTGATCAAGCCAGATATCAGCGATGGCTC
  ATTAAAAACAAAATGAAGGCTTTCTATGCTCCGGTTCACGCAGATGACTTGCGAGAAGGTGCACAATACT
  TGATGCAGGCTGCTGGACTTGGTCGTATGAAGCCAAACACACTTGTCCTGGGATTTAAGAAAGATTGGTT
  ACAAGCCGATATGAGGGATGTGGATATGTATATAAACTTATTTCATGATGCTTTTGACATACAATTTGGA
  GTTGTGGTTATCCGCCTGAAGGAAGGACTGGATATATCACACCTCCAAGGTCAGGAAGAATTATTATCTT
  CACAAGAAAAATCACCTGGTACCAAGGATGTGGTAGTAAATGTGGATTACAGTAAAAAGTCAGATCAAGA
  TACTTGCAAATCATCTGGTGAAAAATCCATTACACAGAAAGATGAGGAAGAGGATGGCAAGACTCCAACT
  CAGCCACTGTTGAAAAAAGAATCCAAAGGCCCTATTGTGCCTTTAAATGTAGCTGACCAAAAGCTTCTTG
  AAGCTAGCACACAGTTTCAGAAAAAACAAGGGAAGAATACTATAGATGTCTGGTGGCTTTTTGATGATGG
  AGGTTTGACATTGTTGATACCCTATCTTCTGACTACCAAGAAAAAGTGGAAAGATTGTAAGATCCGAGTA
  TTCATTGGTGGAAAAATAAACAGAATAGACCATGACCGGAGAGCGATGGCTACTTTATTGAGTAAATTCC
  GGATAGACTTCTCTGATATCATGGTCCTTGGAGATATCAACACCAAGCCAAAAAAAGAAAATATTATTGC
  TTTTGATGACATGATCGAGCCGTACAGACTTCACGAAGATGATAAAGAACAGGACATTGCAGATAAGATG
  AAAGAAGATGAGCCTTGGCGAATCACAGATAATGAGCTTGAGCTTTATAAGACTAAGACATATCGACAGA
  TCAGGTTAAATGAATTATTAAAGGAGCATTCAAGCACAGCTAACATTATTGTCATGAGTCTTCCAGTCGC
  ACGGAAAGGCGCTGTGTCCAGTGCTCTGTATATGGCTTGGTTAGAAGCTCTCTCGAAGGACCTGCCACCA
  GTCCTCCTGGTTCGTGGGAATCATCAGAGCGTCCTTACCTTCTACTCATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009194
• Insert Size: 3621 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009194.3, NP_033220.2
• RefSeq Size: 6520 bp
• RefSeq ORF: 3621 bp
• Locus ID: 20496
• UniProt ID: P55012
• Cytogenetics: 18 32.15 cM
• Gene Summary: Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume.[UniProtKB/Swiss-Prot Function]