Nek1 (NM_175089) Mouse Untagged Clone

CAT#: MC223912

Nek1 (untagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), (10ug)


  "NM_175089" in other vectors (4)

Reconstitution Protocol

USD 1,153.00

6 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 160.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00

Other products for "Nek1"

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Nek1
Synonyms D8Ertd790e; kat
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC223912 representing NM_175089
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAAGTATGTGAGACTGCAGAAGATTGGAGAAGGTTCATTTGGAAAAGCTGTTCTTGTTAAATCGA
CAGAGGATGGCAGACATTATGTCATCAAGGAAATTAACATCTCAAGAATGTCTGATAAAGAAAGGCAAGA
ATCAAGGAGAGAAGTTGCTGTATTGGCAAACATGAAGCATCCAAATATTGTCCAATATAAAGAATCATTT
GAAGAAAATGGCTCTCTCTACATAGTAATGGATTACTGTGAAGGAGGTGATTTGTTTAAACGAATAAATG
CTCAGAAAGGCGCTCTGTTTCAAGAAGACCAGATTTTGGACTGGTTTGTGCAGATATGTTTGGCTCTGAA
GCATGTACATGATAGAAAAATTCTTCACCGAGACATAAAGTCACAGAACATATTTCTAACCAAAGATGGG
ACAGTGCAGCTTGGAGATTTTGGAATTGCTCGAGTTCTTAATAGTACTGTAGAGCTGGCTCGAACTTGCA
TAGGCACTCCATACTACTTGTCACCTGAAATCTGTGAAAACAAGCCTTATAACAATAAAAGTGACATTTG
GGCTTTGGGCTGTGTCCTTTATGAGTTGTGTACACTTAAACATGCATTTGAAGCTGGAAACATGAAAAAC
CTGGTACTGAAGATAATCTCCGGATCCTTTCCTCCAGTGTCTCCACATTACTCCTATGATCTCCGCAGCT
TGCTGTCTCAGTTATTTAAAAGAAATCCTAGGGATAGACCATCAGTCAACTCCATATTGGAGAAAGGTTT
TATAGCTAAACGAATCGAAAAGTTTCTCTCCCCTCAGCTTATTGCAGAAGAATTTTGTCTAAAAACACTT
TCAAAGTTTGGACCACAGCCTCTCCCAGGTAAAAGACCAGCATCAGGACAAGGTGTCAGTTCTTTTGTCC
CTGCTCAGAAAATCACAAAGCCTGCTGCTAAATACGGAGTGCCTTTAACATATAAGAAGTATGGAGATAA
AAAGTTACTTGAGAAAAAACCACCCCCAAAACATAAACAGGCCCATCAAATTCCCGTGAAGAAAATGAAT
TCTGGAGAAGAAAGGAAGAAAATGTCTGAGGAAGCAGCAAAAAAAAGAAGGTTGGAATTTATTGAGAAAG
AAAAGAAGCAAAAGGATCAGATTAGGTTCCTGAAGGCTGAGCAGATGAAGCGGCAAGAGAAGCAGCGGTT
GGAGAGGATAAATAGGGCCAGGGAACAAGGATGGAGGAATGTTTTAAGGGCTGGTGGAAGCGGTGAAGTA
AAGGCTTCCTTTTTTGGCATTGGAGGGGCTGTCTCTCCATCACCGTGTTCTCCTCGAGGCCAGTATGAAC
ATTACCATGCCATTTTTGACCAAATGCAGCGGCTAAGAGCAGAAGATAATGAAGCAAGATGGAAGGGGGG
AATCTATGGTCGATGGCTCCCAGAAAGGCAAAAAGGACACTTAGCTGTAGAGAGAGCCAACCAAGTGGAA
GAATTCCTACAGCGTAAACGAGAAGCTATGCAGAATAAAGCCCGAGCCGAAGGACACGTGGTTTATTTGG
CAAGACTGAGGCAAATAAGACTACAAAATTTTAATGAGCGCCAACAGATTAAAGCCAAACTTCGTGGTGA
GAATAAAGAAGCTGATGGTACCAAAGGACAAGAAGCAACTGAAGAGACTGACATGAGGCTCAAAAAGATG
GAGTCACTTAAGGCGCAAACAAATGCACGTGCTGCTGTACTAAAAGAACAGCTGGAGCGAAAAAGAAAGG
AAGCTTATGAAAGAGAAAAGAAAGTATGGGAAGAACATTTGGTGGCGAGGGTAAAAAGCTCAGATGTTCC
TCTGCCTTTGGAACTTCTTGAAACAGGTGGTTCTCCATCAAAGCAGCAGGTGAAGCCTGTCATTTCTGTG
ACTTCAGCTTTGAAAGAAGTGGGCCTGGATGGAAGTTTAACTGATACCCAGGAAGAAGAAATGGAAAAGA
GTAACAGTGCTATTTCAAGTAAGCGAGAAATCCTGCGTAGGCTAAATGAAAATCTTAAAGCTCAAGAGGA
TGAAAAGGAAAAGCAGCATCACTCAGGTTCTTGTGAGACCGTTGGTCACAAAGATGAGAGAGAGTATGAG
ACAGAAAATGCCATTTCCTCTGATCGCAAGAAGTGGGAGATGGGAGGTCAGCTTGTGATTCCTCTCGATG
CAGTGACACTGGATACATCCTTCTCTGCAACCGAAAAACATACTGTGGGAGAGGTTATTAAATTAGATTC
TAATGGCTCTCCAAGAAAAGTCTGGGGGAAAAACCCTACAGATTCTGTGCTGAAGATACTTGGAGAAGCT
GAATTACAGCTACAGACAGAACTACTAGAAAACACATCTTTTAAAAGTGAGGTTTATGCTGAAGAGGAGA
ACTACAAACCCTTACTTACTGAAGAAGAGAATCTGCAGTGCATTTCAAAAGAAATAAATCCATCAGCTAC
TGTTGATTCTACTGAAACGAAAAGTCCAAAGTTTACTGAGGTGTCTCCACAAATGTCAGAAGGAAATGTG
GAAGAACCTGATGATTTGGAAACAGAAGTTCTACAAGAGCCAAGTAGCACACACACAGATGGGAGTTTGC
CACCTGTTCTTAATGATGTGTGGACTAGAGAGAAGGAAGCAGCTAAGGAAACTGAGTTGGAAGATAAGGT
TGCTGTGCAGCAGAGTGAAGTTTGTGAAGATAGAATTCCAGGGAACGTGGACCAATCCTGTAAGGATCAG
AGAGATCCTGCAGTAGACGATTCTCCGCAGTCTGGCTGTGATGTAGAGAAGTCAGTACAGCCAGAATCGA
TTTTCCAGAAAGTGGTTCATTCTAAGGACTTGAACTTAGTTCAGGCAGTTCATTGCTCACCAGAAGAACC
AATTCCAATTCGATCTCACTCTGATTCTCCACCAAAAACTAAGAGCAAGAATTCCTTACTGATTGGACTT
TCAACTGGTCTGTTTGATGCAAACAATCCAAAGATGCTGAGGACCTGCTCACTTCCAGATCTTTCCAAGC
TGTTCAGAACCCTAATGGACGTTCCCACTGTGGGGGACGTTCATCAAGACAGTCTTGAAATCGATGAGCT
GGAAGATGAACCAATTAAAGAAGGGCCTTCTGATTCCGAAGACACTGTATTTGAAGAAACTGACACAGAT
TTACAAGAGCTTCAGGCCTCAATGGAGCAGCTGCTTAGGGAGCAACCAGGTGACGAATACAGTGAGGAGG
AAGAGTCTGTTTTAAAAAGCAGCGATGTGGAGCAGACAGCAAGAGGGACAGATGCCCCAGACGAGGAGGA
CAACCCCAGCAGCGAAAGCGCCCTGAACGAGGAATGGCACTCAGATAATAGTGACGCTGAGACCACTAGT
GAATGTGAATATGACAGTGTCTTTAACCATTTAGAGGAACTAAGACTTCACTTGGAGCAAGAAATGGGCT
TTGAAAAGTTCTTTGAGGTTTATGAGAAAGTAAAGGCTATTCATGAGGATGAAGATGAAAATATTGAAAT
TTGTTCAACAATAGTTGAGAATATTTTGGGCAATGAGCACCAGCATCTCTATGCCAAGATTCTGCATTTA
GTCATGGCAGATGGAGCCTATCAGGAAGATAATGATGAATAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_175089
Insert Size 3612 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_175089.4, NP_780298.2
RefSeq Size 5419 bp
RefSeq ORF 3612 bp
Locus ID 18004
UniProt ID P51954
Cytogenetics 8 30.91 cM
Gene Summary Phosphorylates serines and threonines, but also appears to possess tyrosine kinase activity (PubMed:1382974). Involved in DNA damage checkpoint control and for proper DNA damage repair (PubMed:18843199). In response to injury that includes DNA damage, NEK1 phosphorylates VDAC1 to limit mitochondrial cell death (By similarity). May be implicated in the control of meiosis (PubMed:1382974). Involved in cilium assembly (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (3) lacks two alternate in-frame exons, compared to variant 1. The encoded protein (isoform 3) is shorter, compared to isoform 1.

Other Versions

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.