Grm1 (NM_016976) Mouse Untagged Clone

CAT#: MC223910

Grm1 (untagged) - Mouse glutamate receptor, metabotropic 1 (Grm1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Grm1
• Synonyms: 4930455H15Rik; ENSMUSG00000075319; Gm10828; Gprc1a; mGluR1; nmf373; rcw; wobl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223910 representing NM_016976
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCCGGCTCCTCTTGATTTTCTTCCCAATGATCTTTTTGGAGATGTCCATTTTGCCCAGGATGCCTG
  ACAGAAAAGTACTGCTGGCAGGTGCCTCGTCCCAGCGCTCTGTGGCGAGAATGGACGGAGATGTCATCAT
  CGGAGCCCTCTTCTCAGTCCATCACCAGCCTCCAGCCGAAAAGGTACCGGAAAGGAAGTGTGGGGAGATC
  AGAGAACAGTATGGTATCCAGAGAGTGGAGGCCATGTTCCACACATTAGATAAGATTAATGCGGACCCGG
  TGCTCCTGCCCAACATCACTCTGGGCAGTGAGATCCGGGACTCCTGCTGGCACTCTTCTGTGGCTCTGGA
  ACAGAGCATTGAGTTCATCAGAGACTCCCTGATTTCCATCCGAGATGAGAAGGATGGACTGAACCGCTGC
  CTGCCTGATGGCCAGACCCTACCCCCAGGCAGGACTAAGAAGCCTATTGCTGGAGTGATCGGCCCTGGCT
  CCAGCTCTGTGGCCATTCAAGTCCAGAATCTTCTGCAGCTGTTCGACATCCCACAAATCGCCTATTCTGC
  CACGAGCATAGACCTGAGCGACAAAACTTTGTACAAATACTTCCTGAGGGTGGTCCCTTCTGACACTTTG
  CAGGCAAGGGCGATGCTTGATATCGTCAAGCGGTACAACTGGACCTATGTCTCTGCAGTCCACACAGAAG
  GGAATTACGGCGAGAGTGGAATGGATGCTTTCAAAGAGTTGGCTGCCCAGGAAGGACTCTGCATCGCACA
  CTCGGACAAAATCTACAGCAATGCTGGCGAGAAGAGCTTTGATCGGCTTCTGCGCAAGCTCCGAGAGCGG
  CTTCCCAAGGCCAGGGTTGTGGTCTGCTTCTGCGAGGGCATGACAGTGCGGGGGTTACTGAGTGCCATGC
  GCCGCCTTGGCGTCGTGGGAGAGTTCTCACTCATTGGAAGTGATGGATGGGCAGACAGAGATGAAGTCAT
  CGAAGGCTATGAGGTGGAAGCCAATGGGGGAATCACAATAAAGCTGCAGTCTCCAGAGGTCAGGTCGTTT
  GATGACTACTTTCTGAAGCTGAGGCTGGACACCAACACGAGGAATCCTTGGTTCCCTGAGTTCTGGCAAC
  ATCGCTTCCAGTGTCGCCTACCTGGACACCTCTTGGAAAACCCCAACTTTAAAAAAGTCTGCACAGGAAA
  TGAAAGCTTGGAAGAAAATTATGTCCAGGACAGCAAAATGGGATTTGTCATCAACGCCATCTATGCCATG
  GCACACGGCCTACAGAACATGCACCATGCTCTATGTCCTGGCTACGTGGGCCTTTGTGATGCCATGAAGC
  CCATTGACGGCAGGAAGCTCCTGGATTTCCTCATCAAATCCTCTTTTGTTGGAGTGTCTGGAGAGGAGGT
  CTGGTTCGATGAGAAGGGGGATGCACCTGGAAGGTATGACATTATGAATCTGCAGTACACAGAGGCTAAT
  CGCTATGACTATGTCCATGTGGGAACCTGGCATGAAGGTGTGCTGAATATCGATGATTACAAAATCCAGA
  TGAACAAAAGCGGAATGGTACGATCTGTGTGCAGCGAGCCTTGCTTAAAGGGTCAGATTAAGGTCATACG
  GAAAGGGGAAGTGAGCTGCTGCTGGATCTGCACAGCCTGCAAAGAGAATGAGTTTGTGCAAGATGAGTTT
  ACCTGCAGAGCCTGTGACCTGGGGTGGTGGCCCAATGCAGAGCTCACAGGCTGTGAGCCCATTACTATCC
  GTTACCTCGAGTGGAGTGACATAGAATCCATCATAGCCATCGCCTTTTCTTGCCTGGGCATCCTCGTGAC
  GCTATTTGTCACCCTCATCTTTGTGCTGTACCGGGACACACCTGTGGTCAAATCCTCCAGTAGAGAGCTC
  TGCTATATCATTCTGGCTGGTATTTTCCTCGGCTACGTATGCCCTTTCACCCTCATCGCCAAACCTACTA
  CCACATCCTGCTACCTCCAGCGCCTCCTAGTTGGCCTCTCTTCTGCCATGTGCTACTCTGCTCTTGTGAC
  CAAAACCAATCGTATTGCACGCATCCTGGCTGGCAGCAAGAAGAAGATCTGTACCCGGAAGCCCAGGTTC
  ATGAGCGCTTGGGCCCAAGTGATCATAGCCTCCATTCTGATTAGTGTACAGCTGACACTAGTGGTGACCT
  TGATCATCATGGAGCCTCCCATGCCCATTTTGTCCTACCCGAGCATCAAGGAAGTCTATCTTATCTGCAA
  TACCAGCAACCTGGGTGTAGTGGCACCTGTGGGTTACAACGGACTTCTCATCATGAGCTGTACCTACTAT
  GCCTTCAAGACCCGCAACGTGCCGGCCAATTTCAATGAGGCTAAATACATCGCCTTCACTATGTACACCA
  CCTGCATCATCTGGCTGGCTTTTGTTCCCATTTACTTTGGGAGCAACTACAAGATTATCACTACCTGCTT
  CGCAGTGAGCCTCAGTGTGACGGTGGCCCTGGGCTGCATGTTCACTCCCAAGATGTACATCATTATTGCC
  AAACCCGAGAGGAATGTCCGCAGTGCCTTCACCACCTCTGATGTAGTGCGCATGCACGTCGGTGACGGCA
  AGCTGCCATGCCGCTCCAACACCTTCCTCAACATTTTCCGGAGAAAGAAGCCTGGGGCAGGGAACGCCAA
  TTCTAACGGCAAGTCTGTGTCATGGTCTGAACCAGGTGGAAGACAGGCGCCCAAGGGACAGCATGTGTGG
  CAGCGCCTCTCTGTGCACGTGAAGACCAATGAGACGGCCTGTAACCAAACAGCCGTAATTAAACCCCTCA
  CTAAAAGTTACCAAGGCTCTGGCAAGAGTCTGACCTTTTCAGACGCCAGCACCAAGACCCTTTACAACGT
  GGAGGAAGAGGACAATACCCCTTCAACTCACTTCAGCCCTCCCAGCAGCCCTTCCATGGTGGTGCACCGA
  CGCGGGCCACCCGTGGCCACCACGCCACCTCTGCCACCCCACCTTAGCGCAGAAGAGACGCCCTTGTTCC
  TGGCTGATTCTGTCATCCCCAAGGGCTTGCCTCCTCCTCTCCCGCAGCAGCAACAGCAGCCGCCCCCTCA
  GCCGCCTCCGCAGCAGCCCAAATCCCTGATGGACCAGCTGCAAGGCGTGGTCACCAACTTTGGCTCTGGG
  ATTCCAGACTTCCACGCGGTGCTGGCAGGCCCGGGGACACCAGGGAACGGGCTGCGCTCCCTGTACCCGC
  CCCCGCCTCCACCGCAACACCTGCAGATGTTGCCCCTGCAGCTGAGCACCTTCCGAGAGGAGCCCATCTC
  CCCTCCGGGGGAGGACGACGATGATGACAGCAGCGAGAGGTTCAAGCTCCTGCAGGAGTTCGTGTATGAG
  CGTGAAGGGAACACGGAGGAAGACGATCTGGAAGAGGAGGAGGACCTGCCTGCAGCCAGCAAGCTGACGC
  CTGAGGATTCGCCTGCCCTGACGCCCCCTTCTCCTTTCCGAGATTCCGTGGCCTCTGGCAGCTCAGTGCC
  CAGTTCCCCAGTGTCTGAGTCAGTGCTCTGCACCCCTCCAAATGTGACCTATGCCTCTGTCATTCTGAGG
  GACTACAAGCAAAGCTCTTCCACTCTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_016976
• Insert Size: 3600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016976.2, NP_058672.1
• RefSeq Size: 4211 bp
• RefSeq ORF: 3600 bp
• Locus ID: 14816
• UniProt ID: P97772
• Cytogenetics: 10 A1
• Gene Summary: G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum (By. similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (alpha). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.