Ccdc136 (NM_145574) Mouse Untagged Clone

CAT#: MC223900

Ccdc136 (untagged) - Mouse coiled-coil domain containing 136 (Ccdc136), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ccdc136
• Synonyms: 4921511K06Rik; BC006583
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223900 representing NM_145574
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAAGCATCGGGGCCTGAGCCTGACAGAGACAGAGCTGGAGGAGCTGAGGGCCCAGGTGCTGCAGC
  TGGTGGCAGAGCTGGAGGAGACCCGTGAGCTTGCTGGGCAGCATGAAGATGACTCCCTGGAGCTTCAGGG
  GCTCCTGGAGGATGAGCGGCTGGCCAGTGCCCAGCAAGCAGAAGTATTCACCAAGCAGATTCAGCAGCTC
  CAAGGTGAGCTGCAACATCTACGGGAGGAGATTTCCCTGTTAGAGCATGAGAAGGAAAGCGAACTTAAAG
  AAATGGAGCAGGAGTTGCATTTGGCCCAAGCAGAGATCCAAAATCTACGGCAAGCTGCGGCAGACTCTGC
  CACTGAACATGAGAGTGACATAGCGTCCTTGCAGGATGATCTCTGCCGGTTGCAGAATGATCTCGATGAC
  ATGGAGCGCATTCGAGGGGACTATGAGATGGAGATTGCCTCGCTCCGGGCAGAAATGGAATTGAAGACTT
  CTGAACCATCTAATCTAAGCATCTCCGACTTCTCTGGGATACAAGATGAACTACACCATCTTCGGGAGCG
  CTACAACTTACTGAATGAGGAGTATCAGGCCTTGCGGGAGAGCAACAGCAGCCTCACAGGGCAGCTTGCT
  GAGCTGGAGAGTGATAGGACACGGAGAGCAACAGAGCGATGGTTGGAATCTCACCTGCTACGGAGTACGA
  TGTCCTCCGAGTCTCAGACGTCAGAACTGGATTTCCCAGAGCCTGACCCTGTGATGCAGCTTTTGCGCCA
  GCAGCTGCTGGGAGCTGAGGAACAGATGCAGGACATGCAGGACAAGTGTAAGAACTTGTATTGTGAATTG
  GAAGAGCTACAGCATCACCGCAGAACCAGTGAGGAGGAGCAGAAGCGGCTGCAGAGGGAGCTCAAGTGTG
  CCCAGAATGAGGTGCTCCGGTTTCAGACCTCCCACAGCACCCAGCATGAGGAGCTGAAGAGCAGGCTCTG
  TACCCTGCAGCAGAAGTATGACGCTAGCCAGGACGAGCACAGTGAGCTTCTGAAGGTGCAGATGCAGCTC
  GAGACTGAGCTCCAGCAGCTCAGACTCCTCAGATGCACTCCTGTAGAGAGCCAGAGTGAGAAGGAGTTGA
  TGTGCCGGCTCCAGAAGCTGCAGGCCCAGCACCAGTGCAGTGTGAATGAGAAAGAGCAGCTTCTAGAAGT
  CCAGCATCACCTGCACGACAAGCTGCGGTGCCACGAGTCAGAGGTGCATCGGCTCCGAAGCATGGTGGAC
  TGCTTGCGAGAGAAAAATGAGAAGAATTCAGGGATACACCTCCAGCTTCAGGAGATGAAGGGATTGTATC
  AGTTCAGCAGGGATGAGCTGGAGCGCCAGAAGCACATGTATGACCAGCTGGAGCAGGACTTCCTGCTCTG
  CCAGCAGGAGCTGACAGAGCTCAAGTCCAGCCAGTCCCTCTGTGAAGAGAATGGAAACTGCTCAAACAAG
  TGTGATGCTCTGCTGGCCAGACTGACAGAATTGCAGGACAAGTTCAAAGCCAGCCAGGAGGAGATTGGGC
  ACCTGCAGATGGAACAGTGTGAGCTCCTGGAGGACCAGAGGAGGCTGCAGGAGGAGCAGGGCCAGCTGCA
  GGAAGAGCTGCACAGGCTCACATTCCCACAGCCCAAATGTGGCATCTTGCAGAAGAGTCAGGAGCTGCTT
  TCAAAGCTGCAAGATCTGTGTGAAATGCAGCTGCTCTACCAAAACATGCAGGAGCAACAGCGAAAGCTGA
  CACAAAACCAGGAGTGTGTACTGAAGGAGCAGCTAGAGGCACACAAGCATCTTCGAGGTTTCAAAGAGTC
  TCATTTCCAGGAAGTGTTGGCGAACCCTCAAGATGCTAGAGGGCCTAAGTCCTCCAGTTGTGAGAATAAG
  TTCAAGGTGCTTATGGACCAGCTGCAGGCTCTGCAGGTGCTATATGACACCAGTCAGAAACAGCAGGAGG
  TACTGCAGCGGGAGCATGGGCGGCTCATGGAGGAGCGGAAGAGGCTGCAGGCTGAGCTACAGCTCTGCAT
  GGAAGAAATGCAGGTGCTCCAAACTCAGTCCCCCATGATAAAAAGGAGTTTTGAGTACTGCGGGAAGAAC
  TCAGGCAGCAGGGCCCCCAGCACTGAGAACTTCCACAGGAGTTACGAGAGCTCCATTGATGAAAATGAGG
  GCTATCAGAAGAGTTATGTGAGCTCCCAGCCCAGCACTGAAACCTTCCTCAAGAGCTATGACAGTAGCAC
  CAGTGCCAACGAGGCCTTTGAGAAGAGTTACTGCTCCAGCAGCACAAGTGTGAGCTATAAGAAGAGTTAT
  GGCTCTGTCAGTAGTGGTGAGACCCTTCACAGGAGCTATGCCAGCAGCAGTACTGATGAGGACCCAGCTG
  AGCCTGAAGACTTGGAGCACTTTGAGGAAACAGTGGCCAAGGTGCTGACCAAGTTGCAGGCAGTGAAGGC
  CCTGTACCAAGTGAGCCAGGAAGAGCACTGCCAGCTGCAGCAACGGATGCACAGGCTGCTGGCCAAGCAG
  AAGGAGCTCACAGAGGAGCTGCAGTGCTGCGAGAAGGAGCTGAGGGAGTGCATGGAGAGCCTGGGGAAGC
  CCCTGCCTCCCCAGAGTGACAAGTGCGAGATCAAAGAACTGCAAACTAAGCTGCGGGAGCTGCAGCTGCA
  GTACCAGGCTAGCATGGATGAGCAGGGGCGGCTTCTGGCCGTGCAGGAGCAGCTGGAGGGGCAGCTGCAG
  TGCTGCCAGGAAGAACTCCGCCAGCTCAAAGAAAACAGGCCCTCTATTAGCTCAGAAGCCAGGGGCAAGA
  ATGTCAATAAGAATATGAACAAGAATGCCAATGGAGTTAGAAATAAGAAGCTGAGCATGGCGTGCTCAGA
  GGATTTGGAGAACGGTTTTGAGAATGAAAAGAACATGTTTGGGATGTGGAAGCCTATGGTTTTCTTGGCT
  ATTGCAGCAGTAGCTCTGTATGTGTTACCCAATATGCGACCGCAGGAATCAGAATACTACATGAAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_145574
• Insert Size: 3009 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145574.3, NP_663549.3
• RefSeq Size: 3507 bp
• RefSeq ORF: 3009 bp
• Locus ID: 232664
• UniProt ID: Q3TVA9
• Cytogenetics: 6 A3.3
• Gene Summary: May play a role in acrosome formation in spermatogenesis and in fertilization.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) differs in the 5' UTR and has multiple coding region differences, compared to variant 3. These differences cause translation initiation at a downstream AUG and result in an isoform (1) with a shorter N-terminus, compared to isoform 3.