Arid5b (NM_023598) Mouse Untagged Clone

CAT#: MC223891

Arid5b (untagged) - Mouse AT rich interactive domain 5B (MRF1-like) (Arid5b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arid5b
• Synonyms: 4930580B11; 5430435G07Rik; AI467247; Desrt; Mrf2; Mrf2alpha; Mrf2beta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223891 representing NM_023598
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCAACTCACTCCAGTGGGTCGGCTCACCGTGTGGCTTGCACGGACCTTATATTTTTTATAAGG
  CTTTTCAATTCCACCTTGAAGGCAAACCAAGAATCTTGTCCCTTGGCGACTTTTTCTTTGTAAGATGTAC
  GCCAAAGGATCCGATTTGCATAGCGGAGCTCCAGCTGTTGTGGGAAGAAAGGACCAGCCGGCAACTTTTA
  TCCAGCTCGAAACTTTATTTCCTCCCCGAAGACACTCCCCAGGGCAGAAATAGCGACCATGGCGAGGATG
  AGGTCATCGCTGTCTCTGAAAAGGTGATTGTTAAATTAGAGGACCTTGTCAAATGGGCGCATTCTGATTT
  CTCCAAGTGGAGGTGTGGGCTCCGAGCCACTCCAGTGAAAACTGAGGCCTTCGGGAGAAACGGACAGAAG
  GAGGCTCTGCTCAGGTACAGGCAGTCAACCCTCAACAGTGGGCTCAACTTCAAAGACGTTCTCAAGGAAA
  AAGCTGATCTCGGGGAAGATGAGGAAGAAACCAACGTGATTGTGCTCAGTTACCCCCAGTACTGTCGGTA
  CCGGTCCATGCTTAAACGGATCCAGGATAAGCCATCGTCCATCCTCACGGACCAGTTCGCATTAGCCCTG
  GGGGGCATCGCAGTGGTCAGCAGAAACCCCCAGATCCTGTACTGCCGGGATACCTTTGATCACCCCACTC
  TGATAGAAAACGAGAGTGTGTGTGATGAGTTCGCGCCAAATCTTAAAGGCAGACCACGCAAAAAGAAAAC
  ATGTCCCCAGAGAAGAGATTCCTTCAGTGGCTCAAAGGACCCCAACAATAACTGTGACGGTAAAGTTATC
  AGCAAGGTGAAAGGAGAGGCCAGGTCAGCCTTGACCAAGCCGAAGAATAACCACAATAACTGTAAAAAAA
  CCTCAAATGAAGAAAAGCCAAAGCTGTCTATTGGCGAAGAATGCAGGGCAGATGAACAAGCCTTCTTAGT
  GGCCCTGTATAAATACATGAAAGAAAGAAAAACGCCCATCGAGCGAATTCCCTACTTGGGTTTCAAACAG
  ATTAACCTTTGGACTATGTTTCAAGCTGCTCAAAAACTGGGAGGATATGAAACAATAACAGCCCGCCGTC
  AGTGGAAACATATTTATGACGAGTTAGGTGGTAATCCTGGGAGCACCAGTGCGGCCACCTGTACTCGCAG
  ACACTACGAAAGGTTAATCCTGCCATACGAAAGATTTATTAAGGGAGAAGAAGATAAGCCTCTGCCGCCA
  ATCAAACCTCGGAAACAGGAAAACAATACACAGGAAAATGAGAATAAAACAAAAGTCTCGGGAAACAAAC
  GTATCAAACAGGAAATGGCCAAAAACAAGAAAGAAAAAGAGAACACCCCCAAACCCCAAGATACATCTGA
  GGTTTCATCAGAGCAAAGGAAGGAAGAAGAGACGTTAAACCACAAAAGCGCCCCCGAGCCTCTCCCTGCT
  CCTGAAGTAAAGGGGAAACCAGAAGGGCACAAGGATCTTGGAGCGAGGGCCCCAGTGTCCAGAGCAGACC
  CAGAAAAGGCCAATGAAACTGACCAAGGTTCCAACAGCGAGAAAGAGGCCGAGGAGATGGGAGATAAGGG
  CCTTGCCCCTCTGCTTCCAAGCCCTCCCTTGCCTCCTGAAAAAGATTCAGCTCCCACCCCTGGGGCTGGC
  AAACAGCCCCTTGCCTCTCCCAGCACCCAGATGGACTCAAAGCAAGAAGCCAAACCCTGCTGCTTTACAG
  AGAGCCCTGAGAAGGACCTCCAAGGAGCACCCTTCTCCAGCTTCTCTGCCACCAAGCCACCACTGACAAG
  CCAGAACGAGGCAGAGGAAGAGCAGCTTCCTGCCACGGCCAACTACATTGCCAACTGCACTGTGAAGGTG
  GACCAGCTGGGCAGTGACGACATCCACACTGCTCTCAAGCAGACCCCGAAGGTCCTTGTGGTCCAATCAT
  TTGACATGTTCAAAGACAAAGACTTGACCGGCCCCATGAATGAGAACCACGGACTTAACTACACGCCCCT
  GCTTTACTCCCGGGGCAACCCTGGTATCATGTCCCCATTGGCTAAGAAAAAGCTTTTGTCTCAGGTGAGT
  GGGGCCAGCCTCTCCAGCAGCTATCCCTATGGCTCCCCGCCCCCTTTGATCAGCAAAAAGAAACTCATTG
  CCAGGGAAGACCTGTGCTCGGGTTTGTCCCAGGGCCACCACAGCCAAAGCTCTGACCACACGGCCGTCAG
  CCGGCCATCCGTAATCCAACATGTTCAGAGCTTCAAAAACAAGGCCTCCGAAGACAGAAAGAGCATCAAC
  GACATCTTCAAGCACGACAAACTGAGCCGGTCCGATGCTCATCGATGTGGCTTCTCCAAGCACCAGCTCG
  GCTCCCTGGCAGACTCCTACATCCTCAAACAAGAGACACAGGAGGGCAAAGATAAACTGTTAGAGAAGAG
  AGCCGTCTCCCACGCCCATGTGCCCAGTTTCCTGGCCGACTTCTACTCATCCCCGCATCTCCATAGCCTC
  TACCGGCACACAGAGCACCATCTTCACAACGAACAGTCCTCCAAGTATGCCGCTAGGGACGCGTACCAGG
  AATCCGAAAACGGCGCCTTTCTTTCTCACAAGCACCCGGAGAAGATCCACGTCAATTATCTCGCATCTCT
  CCACCTGCAAGATAAGAAGGTGGCAGCGGCAGAAGCCTCTACCGATGATCAACCCACAGATCTGAGCCTT
  CCCAAGAACCCACATAAACTAACCAGCAAAGTCCTGGGCCTGGCCCACTCCACCTCGGGGTCCCAGGAGA
  TCAAAGGCGCCTCCCAGTTCCAGGTCGTCAGCAACCAGAGTCGAGACTGTCATCCCAAAGCCTGCCGGGT
  ATCGCCCATGACCATGTCGGGCCCTAAGAAATACCCTGAGTCACTTGCAAGGTCAGGAAAGCCTCACCAG
  GTGAGACTGGAGAACTTCAGGAAAATGGAAGGCATGGTCCATCCCATCCTGCACCGGAAGATGAGCCCGC
  AGAACATTGGAGCTGCGCGCCCCATAAAGCGCAGCCTGGAGGATTTAGATCTGGTGATCGCCGGGAAAAA
  GGCCAGGGCTGTGTCTCCCTTGGACCCAGCCAAGGAGGCTTCTGGGAAAGAAAAGGCCTCCGAACAGGAG
  AGCGAGGGCAACAAAGGCGCCTACGGTGGGCATTCTGGGGCTGCCTCAGAAGGGCACAAGCTCCCTCTCT
  CCACCCCTATCTTCCCGGGTTTGTATTCTGGGAGCCTGTGTAATTCAGGCCTCAACTCCAGGCTCCCGGC
  CGGGTACTCCCATTCTCTGCAGTACTTGAAGAACCAGACGGTGCTTTCTCCTCTCATGCAACCCCTGGCT
  TTCCACTCGCTCGTGATGCAGAGAGGAATTTTTACATCGCCGACAAATTCTCAGCAGCTGTACAGACACT
  TGGCCGCAGCCACCCCTGTAGGAAGTTCCTACGGGGACCTGTTGCACAACAGCATCTACCCATTAGCTGG
  TATCAACCCTCAGGCTGCCTTCCCATCTTCTCAGCTGTCATCCGTACACCCCAGCACAAAACTGTAA
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-NotI
• ACCN: NM_023598
• Insert Size: 3567 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023598.2, NP_076087.2
• RefSeq Size: 4494 bp
• RefSeq ORF: 3567 bp
• Locus ID: 71371
• UniProt ID: Q8BM75
• Cytogenetics: 10 B5.1- B5.2
• Gene Summary: Transcription coactivator that binds to the 5'-AATA[CT]-3' core sequence and plays a key role in adipogenesis and liver development. Acts by forming a complex with phosphorylated PHF2, which mediates demethylation at Lys-337, leading to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver (By similarity). Required for adipogenesis: regulates triglyceride metabolism in adipocytes by regulating expression of adipogenic genes. Overexpression leads to induction of smooth muscle marker genes, suggesting that it may also act as a regulator of smooth muscle cell differentiation and proliferation.[UniProtKB/Swiss-Prot Function]