Rasgrf2 (NM_009027) Mouse Untagged Clone

CAT#: MC223885

Rasgrf2 (untagged) - Mouse RAS protein-specific guanine nucleotide-releasing factor 2 (Rasgrf2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rasgrf2
• Synonyms: 6330417G04Rik; AW048350; Grf2; Ras-GRF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223885 representing NM_009027
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGAAGAGCGTGCGCTACAACGAGGGGCACGCCCTGTACCTGGCCATGCTGGCGCGCAAGGAGGGCA
  CCAAACGCGGCTTCCTGAGCAAGAAGGCTGCAGAGGCGAGCCGCTGGCACGAGAAGTGGTTCGCTCTCTA
  CCAGAATGTGCTCTTTTACTTCGAAGGCGAGCAGAGCGGCCGGCCAGCGGGCATGTACCTTCTAGAGGGC
  TGCAGCTGTGAGCGGACGCCTGCACCACCCAGGACCAACGCAGGACCCGCAGGCGCCCGGGACGCTTTGG
  ACAAGCAGTATTACTTCACTGTCCTTTTTGGGCACGATGGTCAAAAACCACTGGAGCTGCGCTGTGAGGA
  GGAGCAGGCCGGGAAAGAGTGGATGGAGGCCATTCACCAAGCCAGTTACGCGGACATTTTGATCGAGAGG
  GAAGTGCTAATGCAGAAGTACATTCATCTAGTTCAGATTGTGGAGACCGAGAAGATCGCCACCAACCAAC
  TCCGACATCAACTCGAAGATCAAGATACCGAAATCGAAAGGCTTAAATCAGAGATTGTTGCTCTTAATAA
  GACCAAGGAACGGATGCGTCCGTACCACGTCCATCAAGAGGAGGAGGATCCAGACATCAAGAAGATTAAG
  AAGGTTCAGAGCTTCATGAGAGGGTGGCTGTGTAGGAGGAAGTGGAAGACCATCGTGCAGGACTACATTT
  GTTCTCCCCATGCAGAGAGCATGAGGAAGAGAAACCAGATTGTGTTCACGATGGTGGAGGCCGAGACGGA
  GTATGTACACCAGCTTTACATCCTGGTGAACGGTTTCCTCAGGCCCCTGCGCATGGCAGCCAGCTCCAAG
  AAGCCCCCCATTAACCACGATGACGTCAGCAGCATTTTTCTCAACAGTGAAACAATCATGTTCCTTCATG
  AAATATTCCATCAAGGACTAAAGGCAAGGCTGGCAAATTGGCCTACTTTGGTTTTAGCGGATCTATTTGA
  TATTTTGCTGCCCATGCTGAACATTTATCAAGAATTTGTACGTAATCACCAGTACAGCCTCCAAGTACTT
  GCCAACTGTAAGCAAAACAGAGATTTTGACAAGCTCTTAAAGCAGTATGAAGCCAACCCTGCCTGTGAGG
  GGAGGATGCTGGAGACGTTCTTGACCTACCCAATGTTTCAGATTCCCAGGTATATCATCACGCTGCACGA
  ACTCCTGGCCCACACCCCCCATGAACATGTGGAGAGGAAAAGTCTGGAGTTCGCTAAGTCCAAGCTGGAG
  GAACTGTCCAGAGTGATGCATGATGAAGTCAGTGACACCGAGAACATACGGAAAAATCTCGCCATTGAGA
  GGATGATCGTAGAGGGCTGTGACATTTTGCTGGACACCAGCCAAACTTTTATCCGACAAGGTTCCCTTAT
  TCAAGTGCCTTCTGTGGAGAGGGGGAAACTGAGTAAAGTCCGCCTGGGCTCGCTGTCCCTGAAAAAGGAA
  GGCGAGAGACAGTGTTTCTTATTCACGAAGCACTTTCTGATTTGTACACGAAGTTCAGGAGGAAAGCTGC
  ATTTGCTCAAGACAGGTGGGGTTCTCTCCCTCATTCAGTGTACATTGATTGAGGAGCCCGATGGAAGTGA
  CGATGACCCCAAAGGTTCTGGCCACATGTTTGGACATCTGGATTTTAAAATCGTAGTGGAGCCTCCTGAC
  GCTGCCTCTTTCACTGTGGTCTTATTGGCACCTTCACGCCAGGAGAAGGCTGCATGGATGAGCGACATCA
  GTCAGTGTGTGGACAATATACGATGTAACGGATTGATGACTATAGTCTTTGAAGAGAATTCCAAAGTTAC
  TGTGCCACATATGATTAAGTCTGATGCCCGTCTTCACAAAGACGACACTGACATCTGTTTCAGTAAAACA
  CTCAATTCCTGCAAAGTGCCCCAGATCCGCTACGCCAGCGTGGAGCGCCTCTTGGAGCGGTTGACGGACT
  TGCGGTTTCTGAGCATTGATTTCCTCAACACCTTCCTGCACACCTACCGCATTTTCACCACGGCCACCGT
  AGTGCTGGCAAAGCTCTCAGACATCTACAAGAGGCCTTTCACCTCCATTCCTGTCAGGTCATTAGAGTTA
  TTTTTTGCCACAAGCCAGAACAACAGAGAACATTTGGTAGATGGAAAATCCCCACGTCTCTGTCGCAAGT
  TCTCTTCCCCACCTCCCCTGGCTGTGTCCAGAACATCCTCCCCAGTGAGGGCCAGAAAGCTGTCTTTGAC
  GTCTTCCTTGAACTCAAGGATTGGAGCCTTGGACCTGACTAATTCCTCATCTTCCAGTAGTCCCACCACC
  ACCACCCACAGCCCTGCTGCATCCCCACCACCGCACACTGCTGTCTTGGAGTCTGCACCAGCCGACAAAG
  CGGGAGATTCTGCAGACATGTCACCATGTAGATCCCCCACAACTCCCCGGCACCTCCGCTATCGGCAGCC
  TGGAGGACAGGTAGCCGACAGCGCCCACTGTTCTGTGTCACCTGCTTCTGCTTTTGCAATCGCCACAGCT
  GCAGCAGGACATGGGAGTCCACCAGGATTTAACAACGAGAGAACATGTGACAAGGAGTTCATCATACGGA
  GAACAGCCACCAATCGAGTCCTGAATGTCCTCCGCCACTGGGTCTCAAAGCATGCCCAGGATTTTGAACT
  CAATAATGAACTCAAAATGAATGTTCTAAACTTGCTAGAAGAAGTTCTACGAGACCCGGACCTTCTTCCT
  CAAGAAAGGAAAGCAACGGCGAACATCCTGAGGGCTCTTTCACAAGATGATCAAGATGATATCCACCTAA
  AATTAGAGGATATAATTCAAATGACAGACTGTCCAAAGGCCGAGTGCTTTGAGACGTTGTCAGCCATGGA
  GCTGGCCGAGCAGATCACTCTCCTGGACCACATTGTTTTCAGAAGCATCCCCTACGAAGAGTTTCTTGGG
  CAGGGCTGGATGAAGCTTGACAAGAACGAAAGGACGCCTTACATCATGAAAACCAGCCAGCACTTCAATG
  AGATGAGTAACCTGGTGGCCTCCCAGATAATGAATTATGCCGACATCAGCTCCCGTGCCAACGCCATTGA
  GAAGTGGGTGGCAGTGGCGGACATTTGCCGATGTCTCCACAACTACAATGGTGTTCTGGAGATCACCTCA
  GCCTTAAACAGAAGTGCCATCTACAGGCTGAAGAAAACCTGGGCCAAGGTGTCCAAGCAGACAAAAGCTC
  TAATGGACAAACTTCAGAAGACTGTTTCATCTGAAGGAAGATTTAAAAACCTCAGAGAGACTCTCAAAAA
  CTGTAACCCCCCAGCTGTCCCTTACCTTGGGATGTACTTGACAGATCTGGCATTCATTGAAGAAGGGACA
  CCGAACTTTACCGAAGAGGGCCTTGTCAATTTCTCCAAAATGCGAATGATATCACACATTATTCGAGAGA
  TACGCCAGTTCCAGCAGACCGCTTACCGCATAGACCAGCAGCCAAAGGTCATACAATACCTGCTTGACAA
  AGCCCTTGTCATAGACGAAGATTCACTCTATGAGCTGTCACTCAAAATTGAACCTCGACTCCCTGCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009027
• Insert Size: 3570 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009027.3, NP_033053.2
• RefSeq Size: 7595 bp
• RefSeq ORF: 3570 bp
• Locus ID: 19418
• UniProt ID: P70392
• Cytogenetics: 13 47.43 cM
• Gene Summary: Functions as a calcium-regulated nucleotide exchange factor activating both Ras and RAC1 through the exchange of bound GDP for GTP. Preferentially activates HRAS in vivo compared to RRAS based on their different types of prenylation. Functions in synaptic plasticity by contributing to the induction of long term potentiation.[UniProtKB/Swiss-Prot Function]