Hipk3 (NM_001145824) Mouse Untagged Clone

CAT#: MC223880

Hipk3 (untagged) - Mouse homeodomain interacting protein kinase 3 (Hipk3), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hipk3
• Synonyms: DYRK6; FIST3; mir-1902; PKY
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223880 representing NM_001145824
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCACAAGTCTTGGTCTACCCACCGTATGTTTATCAAACTCAGTCAAGTGCCTTTTGTAGTGTGA
  AGAAACTCAAAGTAGAGCCAAGCGGTTGTGTTTTCCAGGAAAGAACCTATCCTCAGATCCATGTGAATGG
  TAGAAACTTTGGCAATTCTCATCCTTCCACGAAAGGTAGTGCTTTCCAGACAAAGATACCATTTACTAAA
  CCTCGAGGACACAGCTTTTCATTGCAGGCAGGTGCCATTGTTGTCAAAGACACTGCCGGTGCTACAAAGG
  TCCTAGCAGCTCAGGCGCAGCAGGCTGGAGTGGAGGCACCGCGGGCCGTGGTGTGGAGAAACAGGTTACA
  TTTCCTAGAAGGGCCCCAGCGATGCGGGTTAAAGCGCAAGAGTGAGGAGTTGGAGAATCACAGCGGCGCG
  ATGCAGATTGTTGATGAACTGTCCATACTTCCTGCAATGTTGCAAACCAACATGGGAAACCCAGTGACAG
  TTGTGACAGCGACCACAGGATCGAAGCAGAACTGCACCAGCGGGGAAGGCGACTATCAGTTAGTGCAGCA
  TGAAGTCCTTTGCTCTATGAAAAACACGTATGAAGTCCTGGATTTTCTTGGTCGTGGCACTTTTGGCCAG
  GTTGTCAAATGCTGGAAGAGAGGGACAAATGAAATTGTAGCCATTAAAATTTTGAAGAATCACCCTTCGT
  ATGCACGTCAAGGGCAGATAGAAGTGAGCATCTTGGCAAGGCTGAGTACAGAGAACGCTGATGAGTATAA
  CTTTGTGCGAGCCTATGAGTGCTTCCAGCACCGTAACCACACCTGCCTAGTCTTTGAGATGCTGGAGCAG
  AACCTGTATGACTTCCTGAAACAGAATAAATTCAGTCCCCTGCCCCTGAAGGTGATCCGGCCTGTTCTTC
  AGCAAGTGGCCACTGCACTGAAGAAATTAAAAAGTCTTGGTTTAATTCATGCTGACCTCAAACCAGAGAA
  TATTATGTTGGTGGATCCTGTTCGCCAGCCGTACAGGGTTAAAGTGATAGACTTTGGATCGGCCAGTCAT
  GTATCAAAGACTGTTTGCTCAACGTATCTTCAATCTCGCTACTACAGAGCTCCAGAGATTATATTGGGGT
  TGCCGTTTTGTGAAGCCATAGACATGTGGTCACTGGGATGCGTGATCGCAGAGTTATTCCTTGGATGGCC
  ACTCTACCCAGGAGCCTTGGAGTATGATCAGATCCGATACATTTCTCAGACTCAAGGTTTACCAGGAGAG
  CAATTGTTGAATGTGGGTACAAAATCCACAAGATTTTTTTGCAGAGAAACAGATATGTCTCATTCTGGTT
  GGAGGTTAAAGACACTGGAAGAGCATGAGGCAGAGACAGGAATGAAGTCTAAAGAGGCCAGAAAGTACAT
  ATTCAACAGTCTGGACGATATAGTGCATGTGAACACAGTGATGGACTTGGAAGGAGGTGATCTCTTGGCT
  GAGAAAGCTGATAGAAGAGAATTTGTTAATCTGTTGAAGAAAATGTTGCTGATTGATGCAGATTTAAGAA
  TTACTCCAATTGAGACTCTGAACCACCCATTCGTTAATATGAAGCATCTACTAGATTTTCCTCACAGCAA
  CCATGTAAAGTCCTGTTTTCATATCATGGATATTTGTAAGTCTCCGAGTTCATGTGAAACAAATAACCAC
  AGTAAAATGTCACTCTTACGACCAGTCGCATCGAATGGCACTGCTGCTCTAGCAGCAAACTTTACTAAAG
  TCGGGACACTAAGAAGTCAGGCGTTAACCACATCTGCTCACTCTGTTGTGCACCATGGCATACCTCTGCA
  GGCGGGGACTGCACAGTTCGGTTGTGGAGATGCTTTTCATCAGACCCTGATTATCTGTCCCCCAGCTATC
  CAAGGTATTCCTGCAGCACATGGTAAACCCACCAGTTATTCCATAAGGGTAGACAATACAGTTCCGCTTG
  TAACTCAGGCCCCAGCTGTGCAGCCTCTGCAGATCCGACCTGGAGTCCTTTCACAGACATGGTCTGGTCG
  AACACAGCAGATGCTAATACCTGCCTGGCAGCAGGTAACACCCATGGCTCCTGCTGCCGCAACACTAACT
  TCTGAAGGCATGGCTGGTTCTCAGAGGCTTGGAGACTGGGGGAAAATGATTCCACACAGCAATCATTACA
  ACTCGGTGATGCCACCGCCTCTTCTAACCAACCAGATCACATTATCAGCCCCTCAGCCTATCAGCGTGGG
  CATTGCACATGTTGTCTGGCCTCAGCCTGCCACTACCAAGAAAAATAAGTTGTGCCAGAACAGGAGTAAT
  TCATTGCAGAACACCAATATCCCACATTCAGCATTTATTTCTCCAAAGATAATCAGTGGGAAAGAGGTTG
  AGGAAGTAAGTTGTGTAGACACACAGGACAATCATACCTCAGAAGGAGAGGCCGGAACTTGCCGTGAAGC
  GTCTGTCAGACAGGATTCTTCAGTCTCAGACAAACAGCGGCAAACCATCATCATTGCCGACTCCCCGAGT
  CCTGCCGTGAGTGTGATCACCATTAGCAGTGACAGCGATGATGAAGAGACCTCACCCAGACCTTCACTCC
  GAGAGTGTAAAGGTAGTCTAGATTGTGAAGCTTGCCAAAGCACTTTGAATATTGATCGGATGTGTTCACT
  CAGCAGTCCTGATAGCACTCTGAGCACCAGCTCCTCAGGGCAGTCCAGCCCGTCCCCTTGCAAGAGACCG
  AACAGCATGTCAGATGATGAACAAGAAAGCGGGTGTGAGACTGTGGATGGCTCTCCAACATCAGACTCCT
  CGGGGCACGACAGTCCATTTGCAGAGAACAGTTTTGTGGAGGATGCTCATCAGAACACAGAGCTGGGGAC
  CTGTGCTGGCCCAGAAGCCAAGCCAGCTGTTGGCACTGCTGTGGAGCCACCGGTGGGGAGAGAAAGTGGA
  CTGAGTGTTGACGAGCACATGGCAAACACGGATTCTACATGCCAGCCATTGAGAAAAGGCCAGCCTGCCC
  CCGGAAAGCTGCACCAGCCTCCTGCCCTGGGCGCTCGTCAGCAGAAGCCCGCAGCAGCGTTCCCTCAGCA
  GCATTTGAACCTGAGCCAGGTTCAGCATTTCGGAACTGGGCATCAAGAATGGAATGGAAACTTTGGGCAT
  CGAAGACAGCAAGCATATATTCCTACTAGTGTTACCAGTAATCCATTCACGCTTTCTCATGGAAGTCCTA
  ACCACACAGCAGTACATGCCCATCTGGCTGGAAGTACACACCTCGGAGGACAGCCTACTCTGCTTCCATA
  CCCATCATCAGCCTCCCTCAGTAGTGCTGCACCCGTGGCCCACCTCCTAGCCTCTCCGTGTACCTCAAGA
  CCTATGTTACAGCATCCAACTTACAATATCTCCCATCCCAGTGGCATAGTTCACCAAGTCCCAGTGGGCA
  TAAACCCCCGCCTGTTACCATCCCCAACCATTCATCAGACTCAGTACAAACCAATCTTCCCTCCACATTC
  TTACATTGCAGCATCACCTGCGTATACTGGATTTCCATTGAGTCCAACAAAACTCAGCCAGTATCCATAT
  ATGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001145824
• Insert Size: 3576 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145824.1, NP_001139296.1
• RefSeq Size: 7494 bp
• RefSeq ORF: 3576 bp
• Locus ID: 15259
• Cytogenetics: 2 E2
• Gene Summary: Serine/threonine-protein kinase involved in transcription regulation, apoptosis and steroidogenic gene expression. Phosphorylates JUN and RUNX2. Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors. The phosphorylation of NR5A1 activates SF1 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. In osteoblasts, supports transcription activation: phosphorylates RUNX2 that synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is one aa shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.