Atp8b5 (NM_177195) Mouse Untagged Clone

CAT#: MC223871

Atp8b5 (untagged) - Mouse ATPase, class I, type 8B, member 5 (Atp8b5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Atp8b5
• Synonyms: 4930417M19Rik; Feta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223871 representing NM_177195
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAATACGTAAAAGCTTTTGTTTCAGAGATTTCATGGGATTGTAGCTGGTACTGCTCTGCAATGCAAG
  AGAGGAGGAATGAAGACAGACAGAAGGAAGAAGAGGAAAGGATTTTGCAGGCCAACAATAGAAGGTTCAA
  CTCTCTTTTTGAGTATCCGGACAATTCCATCAAGACATCAAAGTATGGTTTCTTTAACTTCCTGCCCATG
  AACCTGTTTGAACAGTTCCAGAGACTTGCAAATGCCTATTTCCTCATTTTGCTCTTCCTACAGTTGGTCC
  CGCAGATCTCCTCCCTGGCCTGGTACACGACCGTGATACCCCTGATTGTGGTGCTATCCATAACTGGAGT
  GAAAGACGCCATCGATGACGTGAAAAGACACCGAAGTGACCAGCAGATCAACAACCGCTCTGTTTCAATC
  CTGGTGAACGGCAGGGTTGAGGAGATCAAGTGGAGGAATGTCCAAGTGGGGGACATAATTAAGCTGGAGA
  ATAACCATCCTGTTACCGCAGACATGCTGTTGCTCTCTAGCAGTGAGCCGTACGGCCTGACATACATTGA
  AACAGCAGACCTGGACGGTGAAACCAACCTGAAAGTGAAGCAAGCCATCTCCGTCACCAGTGCCATGGAG
  GACAACCTGGAGCTGCTGTCCTCCTTTAATGGAGAAGTTAGGTGTGATCCTCCTAATAACAAGCTGGACA
  AGTTTTCTGGAACCCTGAGCTACCTGGGGAACACCTACTTACTGAACCATGAAAGGCTACTGCTCCGAGG
  CTGCGTCATCCGGAACACAGACTGGTGCTACGGTTTGGTCGTTTACACTGGGCAAGACACCAAATTAATG
  CAGAACAGTGGACGGTCCACCTTCAAACGAACACATATAGATCATCTCATGAATGTCCTTGTGGTCTGGA
  TTTTCATGTTTTTAGGTGGAATGTGTTTTCTGCTGTCGATTGGACATGGCATCTGGGAGAACAGCAGAGG
  CTACTATTTCCAGGCCTTTCTCCCATGGAAACATTATATTACCTCATCGGCTACGAGTTCTGCCCTCATT
  TTCTGGTCCTACTTCATAGTGCTCAACACCATGGTACCCATTTCCCTCTATGTCAGTGTGGAAATAATAA
  GACTGGGCAACAGTTACTACATCAACTGGGATCGAAAGATGTTTTACGCCCCAAAGAACATGCCAGCGCA
  GGCTCGCACCACCACTCTTAATGAGGAGCTGGGCCAAGTCCAATACGTGTTCTCTGACAAAACAGGAACC
  CTGACTGAGAATGTGATGATTTTCAACAAATGTTCTATCAACGGGAAGACCTATGGTTATTCCTACGACG
  ACAATGGAGAGTATGTGCCAAAATCTCCAAAAGACAAGGTGGACTTCTCTTACAACCACCTGGCTGACCC
  TAAGTTTTCCTTCTATGACAAGACCTTGGTGGAAGCGGTGAAGAGTGAAGACCCGCTGGTCTACCTGTTC
  TTCCTCTGTCTCTCGCTGTGCCACACGGTGATGTCAGAGGAGAAGGTGGAAGGTGAGCTGGTGTACCAGG
  CTCAGTCACCAGATGAAGGTGCCCTGGTTACGGCTACTAGGAACTTTGGCTTTGTGTTCTGCTCCCGCAC
  ACCTGAGACTATCACAGTGATCGAAATGGGGAAAATCCGAGTTTACCGCCTACTGGCTATCTTGGACTTC
  AGCAATGAGCGCAAGAGGATGTCTGTCATTGTTCGGACACCTGAAGATCGAGTCATGTTGTTCTGCAAGG
  GAGCAGACACCATCATCTATGAGCTGCTGCATCCATCTTGTGCATCCCTTAGTGAAGTGACCATGGACCA
  CTTAGATGATTTTGCCAGCGAAGGCCTTCGCACCCTCATGGTAGCTTACCGAGAGCTGGATAAGGCATAT
  TTCCAGACATGGATTAAAAAGCATGGTGAAGCCTGGCTGACTTTAGAGAATCGAGAAAGGAAACTAGCTC
  TGGTCTATGAAGAAATCGAAAGAGACTTGATGTTACTAGGGGCCACAGCCATAGAAGACAAGCTTCAGAG
  AGGAGTACCTGAGACCATTGTCACCCTGAGCAAAGCCAAAATTAAAATATGGGTTCTGACTGGCGATAAG
  CAAGAGACTGCTGTGAACATCGCCTACTCCTGTAGGATATTCAAGGATGAAATGGATGGGGTGTTCATGG
  TGGAAGGCACAGATAGGGAAACTGTTCTGGAAGAACTCAGAACAGCTAGGAAGAAGATGAAACCCGAGTC
  TCTGCTGGAATCAGATCCCATAAACATGTACCTCGCCAGGAAGCCCAAAATGCCTTTCAAATCACTTGAC
  GAGGTGGCCAACGGCAACTATGGCTTGGTCATCAGTGGCTACAGTCTGGCCTATGCTCTAGAAGGGAGCC
  TGGAGTTCGAGCTGCTGCGGACCGCATGCATGTGCAAGGGCGTGGTCTGCTGCCGGATGACACCCCTCCA
  GAAGGCCCAAGTGGTAGACCTGGTGAAGAGGTACAAGAAGGTGGTGACACTGGCCATCGGGGATGGGGCC
  AATGACATCAGCATGATCAAAGCTGCCCATATCGGGGTTGGCATCAGCAACCAGGAAGGGATGCAGGCCA
  CGCTCAGCAGTGACTTCTCCTTCTGCCAGTTCCACTTTCTTCAGCGCCTGCTCTTGGTGCACGGCCGCTT
  GTCTTACAATCGCATGTGCAAGTTTCTCAGCTACTTCTTTTACAAAAACTTTGCCTTTACCTTGGTGCAC
  TTCTGGTATGCCTTCTTCAACGGCTTCTCTGCGCAGACAGTTTACGACATCTGGTTCATCACCTTCTACA
  ATCTCATCTACACTTCCCTCCCGGTCCTGGGCTTGAGTCTGTTTGAAAAGGATGTGAATGAAACTTGGAG
  TCTGTGTTATCCAGAGCTGTATGAACCAGGACAGCACAACCTGTACTTCAACAAGAAAGAATTTGTGAAG
  TGCTTACTGCATGGCATCTACAATTCCTTCGTGCTGTTCTTTGTCCCCATGGGGACCGTCTTCAACTCAG
  AGCGCAATGATGGGAAGGACATCTCCGACTTCCAGTCCTTCTCCCTGCTAGTGCAGACGACTCTGATTGG
  CGTCATGACAATGCAGATTGCCCTGAGGACAACCTCCTGGACGATGATAAACCACACCTTCACGTGGGGC
  AGCCTGGGGCTCTACTTCTGCATCCTAATCTTACTGTGTAGTGATGGCCTGTGTCTCAGGTACCCCAGCA
  TCTTCAATTTCCTAGGTGTGGCCAGGAACAGCCTTAGCCAGCCACAGATCTGGCTGTGTCTCATCCTCAG
  CACCATCCTCTGCATGATTCCTCTGATTGGATACAACTTTCTCAGACCACTCCTCTGGCCCATCAACGCT
  GACAAGGTTCTGAACAGGATCCATTTTTGCTTGAAACATCCAATACCAACCCAAGTCCAGACCAAAATAA
  AACACCCAAGCCTTCGACGTTCTGCCTACGCGTTCTCCCACAAACAGGGCTTTGGGGCCCTCATCACGTC
  TGGCAAGACGCTGAAATCCAGTGCCTTGGCAAAAAGCAAGAGGTTCTTGTAA
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-NotI
• ACCN: NM_177195
• Insert Size: 3552 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177195.3, NP_796169.2
• RefSeq Size: 4474 bp
• RefSeq ORF: 3552 bp
• Locus ID: 320571
• UniProt ID: A3FIN4
• Cytogenetics: 4 A5
• Gene Summary: P4-ATPase flippase which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. May play a role in phospholid transport across membranes and in acrosome formation.[UniProtKB/Swiss-Prot Function]