Nlrp1a (NM_001004142) Mouse Untagged Clone

CAT#: MC223865

Nlrp1a (untagged) - Mouse NLR family, pyrin domain containing 1A (Nlrp1a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nlrp1a
• Synonyms: CARD7; DEFCAP; Gm14; Gm15; NAC; Nalp1; Nalp1a; Nlrp1; PP1044
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223865 representing NM_001004142
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAATCTCAGTCCAAGCAGGAAAGTAGCACAAAAGTGGCTCAGCATGAAGGTCAAGAGGATGTGG
  ACCCAACCTTCAAAACAAAGAAACTTATGGAGGTGGAGCTAATGAAGCACAGAGTGCAGCTAGAGAGGAA
  CTTGAAGCTAAGAACCTTTCCAGGAGCACGTACGAAGCAAGTAAAAGAGGCTCTTTACCCTCTTCTAACA
  TGGTCATCAAAAAGCAAGAATCTGTTCCAAAATTTCACAAAACTACTACTCTTTAAAAAATTGTGCCAAA
  GAGGCTCAGAAAACTTGGTCAGGGAAAGCTGGTATCCATGTGTACCAGAAGAAGAAGCACATATGATTGA
  CATCCAAGATTTATTTGGCCCAAACTTAGGTACTCAGAAAAAGCCTCAATTAGTCATAATAGAAGGAGCT
  GCTGGGATTGGGAAGTCAACACTGGCCAGACTTGTGAAGAGAGCCTGGAAGGAAGGCAAGCTTTACAGGA
  ATGACTTCCATCATGTCTTCTTCTTCAGCTGCAGAGAGCTGGCCCAGTATGAGCAGCTGAGTCTGGCTGA
  GCTCATAGTACAAGGCCAGGAAGTGCCCACAGCTCCCATTAGGCAGATCCTGTCTCACCCTGAGAAGTTG
  CTCTTCATTCTGGATGGCATAGATGAGCCAGCATGGGTCTTAGCAGACCAGAATCCTGAGTTATGTCTTC
  ACTGGAGTCAAACCCAGCCTGTGCACACTCTGCTGGGCAGTTTACTTGGGAAATCCATCCTTCCTGGGGC
  TTCCTTCTTGCTCACAACTCGCACCACAGCTCTACAGAAATTCATTCCTTCCCTGGAGCAGCCATGTCAA
  GTGGAAGTCCTAGGATTCACTTTGTTTGAACGGAAAAACTATTTCTACAAATATTTTGGGAAGAAAAAAG
  GTGGAGTTACTACTTTTACATTAGTTAAATCAAACTCAGCGCTCCTGACTCTGTGTGAAGTTCCCTGGGT
  GTGCTGGTTGGTCTGCACTTGCCTGAAAAAGCAGATGGAGCAGGGTGGAGAGCTCTCACTGACCTCACAG
  ACCACCACAGCACTCTGCCTGAAATACCTTTCCCTGACGATCCCAGGGCAGCACATGAGGACCCAGCTCA
  GAGACCTCTGCTCACTGGCTGCTGAGGGGGTCTGCCAAAGAAGGACCCTGTTCAGTGAAAGCGATCTCTG
  TAAGCAAGGGTTAGATGAGCATGCCATTGCCAGTTTCCTGAAGATTGGTGTCCTTCAAAAGCAGGCCAGC
  TCTCTGAGCTACAGCTTTGCCCACTTGTGTCTTCAGGAGTTCTTTGCAGCAATGTCCTACATCTTGGATG
  ATAGTGAGGAAAGACATGCTGATATGAAAAATGACAGAATTGTGGAAACACTGGTAGAAAGGTATGGAAG
  ACAAAACCTGTTTGAGGCACCCACTGTGCGTTTTTTATTTGGTCTTTTGAGTAAAGAGGAATTGAAAAAA
  ATAGAAAAGCTCTTTTCCTGCAGCCTTCATGGAAAGACAAAGTTGAAACTACTATGGCACATCCTAGGGA
  AATCCCAACCCCATCAACCACCTTGCCTGGGTTTGCTCCACTGTCTGTATGAAAATCAGGATATGGAGCT
  CCTGACACACGTGATGCATGATCTGCAAGGAACAATAGTGCCTGGACCAGATGATTTGGCACACACAGTG
  TTGCAGACAAACGTGAAGCACCTGGTGATACAGACAGACATGGACCTCATGGTGGTTACTTTCTGCATTA
  AGTTCTGCTGTCACGTGAGGAGTCTTCAGCTGAACAGGAAGGTACAGCAGGGACATAAGTTTACGGCCCC
  TGGGATGGTTCTGTACAGATGGACCCCAATCACTGATGCCAGCTGGAAGATTTTCTTCTCCAATCTTAAG
  TTAGCCAGAAACCTGGAGGAGTTGGACCTAAGTGGAAATCCACTGAGCTACTATGCAGTACACAGTCTTT
  GTACGACCCTGAGAAAGCGTGGCTGCCAACTAAAGACTCTGTGGCTTGTTGAATGCGGCCTCACATCCAC
  ATACTGCTCACTTCTGGCCTCAGTACTCAGTGCCCGCTCCAGCCTGACTGAGCTAGACCTGCAGCTGAAT
  GACCTGGGTGATGGTGGTGTGAAGATGTTGTGTGAGGGGCTCAGGAATCCTGCCTGCAATCTCAGCATCC
  TGTGGCTGGACCAGGCATCTCTCAGTGACCAAGTGATTGCAGAGCTCAGAACTCTGGAGGCAAAGAATCC
  AAAGCTGCTCATCTCCAGCACATGGAAGCCACATGTGATGGTCCCTACGATGAACATGGATAAAGAAGAA
  GTGGGTGATAGCCAGGCCCTACTGAAGCAGCAGAGACAGCAGTCAGGAGACAAACACATGGAACCTCTGG
  GGACTGAAGATGAGTTCTGGGGCCCTACAGGACCTGTGACTACTGAGGTGGTTGACAGAGAGAGGAACCT
  GTACCGAGTTCAGTTACCCATGGCTGGTTCCTACCACTGTCCCAGTACAGGACTCCACTTTGTGGTGACA
  AGGGCAGTGACAATCGAGATTGAATTCTGTGCCTGGAGCCAATATCTCGACAAGACTCCCTTGCAGCAGA
  GTCACATGGTGGTTGGACCTCTGTTTGACATCAAGGCTGAGCAAGGAGCTGTGACTGCAGTGTACCTCCC
  TCATTTTGTGGCCCTCCAAGAGGGAATCGTGGACAGCTCCTTGTTCCATGTTGCCCACTTTCAAGAACAT
  GGGATGGTCCTAGAAACACCAGCAAGGGTGGAACAGCATTACGCAGTACTGGAAAACCCAAGCTTCTCCC
  CAATGGGGATTCTACTGAGAATGATTCCGGCTGTCGGACACTTCATCCCCATCACTTCCACCACACTGAT
  CTACTATCACCTCTATCTTGAAGACGTCACCTTTCACCTTTACCTGGTCCCCAATGACTGCAGTATTCGA
  AAGGCCATTGATGATGAAGAAATGAAGTTTCAGTTTGTGCGCATAAACAAGCCACCCCCAGTAGACGCTC
  TTTATCTTGGTTCCCGCTATATCGTGTCTTCCTCCAAATTGGTGGAAATTATCCCAAAGGAACTAGAGCT
  GTGCTACCGAAGCCCTGGGGAATCCCAGCTCTTCTCTGAGATCGACATTGGGCACATGGATTCAGAGATT
  AAGCTGCAAATCAAAGACAAGAGGCACATGAATCTCAAATGGGAGGCCTTGCTGAAACCAGGGGATCTCA
  GACCTGCACTGCCCAAGATTGCTACAGCCCCCAAAGATGCTCCTTCCTTGCTGCACTTCATGGACCAGCA
  TCGGGAGCAGCTGGTGGCCCGAGTGACATCTGTAGATCCTCTCTTGGACAAGCTGCATGGTCTGGTGCTG
  AGTGAAGATTCCTATGAAGTAGTGCGGTCTGAGACCACAAACCAAGACAAGATGAGGAAGCTCTTCAGCC
  TCAGCCGGTCCTGGAGCTGGGATTGCAAAGACCAATTCTACCAAGCTCTGAAGGAGACCCATCCTCACCT
  GGTCATGGATATACTTGAGAAGTTGGGTGGGGTCTCTGTGAAATCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001004142
• Insert Size: 3549 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004142.2, NP_001004142.2
• RefSeq Size: 4806 bp
• RefSeq ORF: 3549 bp
• Locus ID: 195046
• UniProt ID: Q2LKU9
• Cytogenetics: 11 43.21 cM
• Gene Summary: As a potential sensor component of the NLRP1 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens and other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of Nlrp1b, CASP1, and possibly PYCARD. Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu. Activation of NLRP1 inflammasome is also required for HMGB1 secretion. The active cytokines and HMGB1 stimulate inflammatory responses. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death (By similarity) (PubMed:23219391). When activated in the bone marrow, induces the pyroptosis of hematopoietic stem cells and progenitor cells of both myeloid and lymphoid lineages, hence allowing the removal of damaged cells, and the release of IL1B, which induces granulopoiesis (PubMed:23219391). Binds ATP (By similarity).[UniProtKB/Swiss-Prot Function]