Pcdh12 (NM_017378) Mouse Untagged Clone

CAT#: MC223860

Pcdh12 (untagged) - Mouse protocadherin 12 (Pcdh12), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pcdh12
• Synonyms: Pcdh14; VE-cad-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223860 representing NM_017378
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCTACTTCTGCCATTCCTGCTAGGGCTCTTAGGGCCAGGAAGCTACTTGTTCATTTCAGGGGATT
  GTCAGGAGGTGGCCACTGTCATGGTGAAATTCCAAGTGACAGAGGAAGTGCCGTCTGGCACGGTGATAGG
  GAAACTGTCCCAAGAACTAAGAGTGGAGGAGAGGCGTGGGAAGGCAGGAGATGCCTTCCAGATTCTGCAG
  CTGCCTCAGGCACTGCCGGTTCAGATGAACTCTGAGGACGGCCTGCTCAGCACTTCCAGCCGGCTGGATC
  GGGAGAAGCTATGTCGGCAGGAAGATCCCTGTCTGGTGTCATTTGACGTGCTTGCCACAGGGGCGTCTGC
  TCTAATTCATGTGGAGATTCAGGTGCTAGACATCAATGACCACCAGCCACAGTTTCCCAAAGACGAGCAG
  GAACTGGAAATCTCAGAGAGTGCCTCTCTGCACACACGAATCCCCTTGGACAGAGCTCTTGACCAAGACA
  CGGGTCCTAACAGCTTATATTCCTACTCCCTGTCTCCCAGTGAACACTTTGCCCTGGATGTTATTGTGGG
  CCCTGATGAGACCAAACATGCAGAGCTTGTGGTGGTGAAGGAGTTGGACAGGGAACTCCACTCATATTTT
  GATCTGGTGCTGACCGCCTATGACAATGGGAATCCCCCTAAGTCAGGAATCAGCGTGGTCAAGGTCAATG
  TCCTGGACTCCAATGACAATAGTCCAGTGTTTGCTGAGAGTTCACTAGCACTAGAAATCCCAGAAGACAC
  TGTTCCTGGTACTCTTCTCATAAACCTGACTGCTACAGATCCCGACCAAGGACCCAATGGGGAGGTAGAG
  TTCTTCTTTGGCAAGCATGTGTCCCCAGAGGTGATGAACACCTTTGGCATAGATGCCAAGACAGGCCAGA
  TCATTCTGCGCCAAGCCCTAGATTACGAGAAGAACCCTGCCTATGAGGTGGATGTCCAGGCAAGGGATTT
  GGGTCCCAATTCCATCCCAGGCCATTGCAAAGTTCTTATCAAAGTTCTGGATGTCAATGACAATGCCCCA
  AGCATCCTCATCACGTGGGCCTCCCAGACGTCGCTGGTGTCAGAAGATCTTCCCAGGGATAGCTTCATTG
  CCCTTGTCAGTGCGAATGACTTGGACTCAGGAAACAACGGTCTCGTCCACTGTTGGCTGAATCAAGAGCT
  GGGCCACTTCAGACTGAAAAGGACTAACGGCAACACGTACATGCTGCTCACCAATGCCACACTGGACAGA
  GAGCAGTGGCCCATATATACTCTCACTGTGTTTGCCCAAGACCAAGGACCCCAGCCCTTATCAGCTGAGA
  AGGAGCTCCAAATTCAGGTTAGTGATGTCAATGACAATGCCCCTGTGTTTGAGAAGAGCCGGTACGAGGT
  CTCCACTTGGGAAAATAACCCACCCTCTCTTCACCTCATCACGCTCAAAGCGCATGATGCTGACTTGGGC
  AGTAATGGAAAAGTGTCATACCGTATCAAGGACTCCCCCGTTTCTCACTTAGTCATTATTGACTTTGAAA
  CAGGAGAAGTCACTGCTCAGAGGTCACTGGACTATGAACAGATGGCAGGCTTTGAGTTCCAGGTGATAGC
  AGAGGACAGAGGGCAACCCCAGCTCGCATCCAGCATCTCGGTGTGGGTTAGCCTCTTGGATGCCAATGAT
  AATGCCCCAGAAGTGATTCAGCCTGTGCTCAGTGAAGGCAAAGCCACCCTTTCGGTGCTTGTAAATGCCT
  CCACGGGCCACCTTCTGTTGCCCATTGAGAATCCCAGTGGCATGGATCCAGCAGGTACTGGTATACCACC
  AAAGGCTACCCACAGCCCCTGGTCTTTCCTTTTGTTAACAATCGTGGCTAGGGATGCAGACTCGGGGGCC
  AATGGGGAACTCTTCTACAGCATTCAAAGTGGGAATGATGCTCATCTCTTTTTCCTCAGCCCTTCCTTGG
  GGCAGCTATTCATTAATGTCACCAATGCCAGCAGCCTCATCGGGAGTCAGTGGGACCTGGGGATAGTGGT
  AGAGGACCAGGGCAGCCCCTCCTTGCAGACCCAAGTTTCATTGAAGGTCGTGTTTGTCACCAGTGTGGAC
  CACCTAAGGGATTCTGCTCATGAGCCCGGAGTTCTGAGCACACCAGCACTGGCTTTGATCTGCCTGGCTG
  TACTGCTGGCCATCTTTGGATTGCTCTTAGCCCTGTTCGTGTCCATCTGCAGGACAGAGAGAAAGGATAA
  TAGGGCCTACAACTGTCGAGAAGCTGAGTCGTCATACCGCCACCAGCCCAAGAGGCCCCAGAAACACATT
  CAGAAGGCAGATATCCACCTGGTGCCTGTGCTTAGGGCCCACGAGAATGAGACTGATGAAGTCAGGCCAT
  CTCACAAGGATACCAGCAAGGAGACACTGATGGAGGCAGGCTGGGACTCTTGCCTGCAGGCCCCCTTCCA
  CCTCACACCAACCCTATACAGGACCCTGCGTAACCAAGGCAACCAGGGAGAACTGGCAGAGAGCCAGGAG
  GTACTGCAGGACACCTTCAACTTTCTCTTTAACCATCCCAGGCAGAGGAATGCCTCCCGGGAGAACCTAA
  ACCTTCCTGAGTCCCCACCTGCTGTACGCCAACCACTCTTAAGGCCTCTGAAGGTGCCTGGTAGCCCCAT
  AGCGAGGGCGACTGGAGACCAAGACAAGGAGGAGGCCCCACAGAGCCCACCAGCGTCCTCTGCAACCCTA
  AGACGACAGCGGAATTTCAATGGCAAAGTGTCTCCTAGAGGAGAGTCCGGTCCTCATCAGATTCTGAGGA
  GCCTGGTTAGGCTCTCTGTGGCTGCTTTTGCGGAACGGAACCCGGTGGAGGAGCCTGCTGGGGACTCTCC
  TCCTGTCCAGCAAATCTCCCAGCTGCTGTCCTTGCTGCACCAGGGCCAATTCCAGCCCAAACCAAACCAC
  CGAGGAAATAAATACTTGGCCAAGCCCGGCGGCAGCAGCAGGGGTACCATCCCAGACACAGAGGGCCTTG
  TAGGCCTCAAGCCTAGTGGTCAAGCAGAACCTGACCTGGAAGAAGGGCCCCCGAGCCCGGAGGAGGACCT
  TTCTGTAAAGCGACTTCTAGAAGAAGAGCTGTCGAGCCTGTTGGACCCTAATACAGGTCTAGCCCTGGAC
  AAGCTGAGTCCGCCTGACCCAGCCTGGATGGCGAGATTGTCATTGCCCCTCACCACCAATTACCGAGACA
  ACTTGTCTTCCCCCGATGCTACAACGTCAGAGGAACCGAGAACCTTCCAGACATTCGGCAAGACAGTTGG
  ACCGGGACCCGAGCTGAGCCCAACAGGCACGCGCCTGGCCAGCACTTTCGTCTCGGAGATGAGCTCTCTG
  CTGGAAATGTTGTTGGGGCAGCACACGGTACCAGTGGAAGCTGCGTCCGCGGCTTTGCGGAGGCTCTCGG
  TGTGCGGGAGGACCCTCAGTCTAGACCTAGCCACCAGTGGGGCCTCAGCTTCAGAAGCACAGGGTAGAAA
  GAAGGCAGCTGAGAGCAGACTTGGCTGTGGCAGGAATCTATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_017378
• Insert Size: 3543 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017378.2, NP_059074.2
• RefSeq Size: 5587 bp
• RefSeq ORF: 3543 bp
• Locus ID: 53601
• UniProt ID: O55134
• Cytogenetics: 18 B3
• Gene Summary: Cellular adhesion molecule that may play an important role in cell-cell interactions at interendothelial junctions (PubMed:9651350). Acts as a regulator of cell migration, probably via increasing cell-cell adhesion (By similarity). Promotes homotypic calcium-dependent aggregation and adhesion and clusters at intercellular junctions (PubMed:9651350). Unable to bind to catenins, weakly associates with the cytoskeleton (PubMed:9651350).[UniProtKB/Swiss-Prot Function]